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Citations for Thermo Fisher :
51 - 100 of 10000+ citations for CKLF Like MARVEL Transmembrane Domain Containing 7 CMTM7 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The gB Domain I and Domain I+II were purified using Nickel-NTA resin (Thermo Fisher Scientific), and the gB Domain II was purified with lectin resin (VWR.)
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bioRxiv - Neuroscience 2020Quote: ... the pellet was resuspended in 1ml HBSS containing DiO (7 μM, Thermofisher Scientific). Cells were then incubated at 37°C for 15 min ...
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bioRxiv - Immunology 2022Quote: ... at a density of 7×105 Caco2 cells/well containing EMEM (Gibco BRL) medium supplemented with 20% FBS ...
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bioRxiv - Genetics 2023Quote: ... and (7) mounted in an anti-fade medium already containing Hoechst (Invitrogen #P36981). All of the following primary and secondary antibodies were diluted in 1XPBS containing 5% donkey serum ...
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bioRxiv - Genetics 2022Quote: ... and cloned into the pDEST32 vector containing the GAL4 DNA binding domain (DBD) through Gateway cloning (ThermoFisher, 11789020 and 12538120) and used as the Y2H bait construct ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were immunostained by primary antibodies like neuronal nuclear protein (Neu-N) (Invitrogen, California, USA, 1:500 dilution) (Gusel’Nikova et al. ...
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bioRxiv - Physiology 2022Quote: ... while mitochondrial transmembrane potential levels were monitored by staining with MitoTrackerRed CMXRos (50 nM; Invitrogen, Molecular Probes). Incubations were performed in the dark for 20 minutes in PBS + 2% FBS at RT ...
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bioRxiv - Physiology 2022Quote: ... while mitochondrial transmembrane potential levels were monitored by staining with MitoTrackerRed CMXRos (50 nM; Invitrogen, Molecular Probes). Incubations were performed in the dark for 20 minutes in PBS + 2% FBS at RT ...
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bioRxiv - Immunology 2021Quote: DNA constructs encoding anti-SCARB2 antibody heavy and light chain variable domains (clone JL278) were synthesized (ThermoFisher) and cloned into mammalian expression vectors containing a mouse IGHV signal peptide and IgG1 constant regions ...
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bioRxiv - Genetics 2022Quote: ... elegans genes was cloned into the pDEST22 vector containing the GAL4 activating domain (AD) using the CloneMinerTM cDNA library construction kit (ThermoFisher, A11180). A forward Y2H library screen was performed using the ProQuest Two-Hybrid System (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: ... This oligo was annealed and amplified to a fixed 91nt oligo containing the scaffold domain using Taq polymerase (Invitrogen, Cat. #10342046). Transcription was performed using Hi-SCribe T7 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 500µl cold FACS buffer containing 1µl 7-AAD (Thermo Scientific) and filtered through a 40µm cap filter into FACS tubes ...
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bioRxiv - Biochemistry 2022Quote: ... Proliferation assays using Human Plasma-Like Medium (HPLM) (ThermoFisher, A4899101) were supplemented with 10% dialyzed FBS ...
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bioRxiv - Microbiology 2023Quote: ... ISG MX dynamin-like GTPase 1 (Mx1, Thermo Fisher, Mm00487796_m1) expression was assessed in select samples ...
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bioRxiv - Microbiology 2022Quote: ... Human Plasma-Like Media (HPLM, Thermo Fisher Scientific, Waltham, MA) was used without added HEPES or exogenous hypoxanthine ...
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bioRxiv - Microbiology 2021Quote: ... NPC1 C domain and NPC1 I domain) and the viral protein constructs were co-transfected using Lipofectamine 2000 (Invitrogen) following the manufacture instructions.
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bioRxiv - Biophysics 2020Quote: Wild-type PSD-95 PDZ3-SH3-GK (PSG), PDZ3 domain and mutants (all pseudo, engineered F337W in PDZ3 domains) are encoded in modified pRSET vector (Invitrogen) and transformed in Escherihia coli BL21(DE3 ...
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bioRxiv - Immunology 2021Quote: The COVID-19 receptor-binding domain (RBD) and the N-terminal peptidase domain of human ACE2 were expressed using HEK293F cells (Invitrogen). The COVID-19 RBD (residues Arg319-Phe541 ...
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bioRxiv - Developmental Biology 2020Quote: ... A pMA-T vector encoding for a DNA in which the DSG domain of Arpp19 sequence was exchanged by the cassette domain (D2-D1 mutant) was synthesized by Geneart (Thermofisher) and sub-cloned into Pet-15 6his vector.
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bioRxiv - Immunology 2022Quote: ... AD-2 domain site 1 (Life Technologies Corporation), Domain I ...
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bioRxiv - Systems Biology 2019Quote: BT-549 and MCF-7 cells were cultured in full media containing RPMI-1640 (Gibco), 10% heat-inactivated fetal bovine serum (FBS) ...
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bioRxiv - Genomics 2021Quote: ... exchanged daily for 7 days and then substituted by STEP2 medium containing Neurobasal medium (Gibco) with 2 % MACS NeuroBrew-21 w/o Vitamin A (Miltenyi Biotec) ...
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bioRxiv - Genetics 2020Quote: ... 25 μL of media containing STS and CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher) at final treatment concentrations of 1 μM and 2.5 μM ...
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bioRxiv - Molecular Biology 2023Quote: A synthetic sequence containing the ORF of KOFP-7 was obtained by GeneArt (ThermoFisher Scientific). This sequence contained several attributes ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... samples were incubated with 5 µg/mL of the DyLight488-conjugated anti-FLAG M1 antibody and the viability dye 7-Aminoactinomycin D (7-AAD, Invitrogen) for 45 min on ice and were then analyzed on a Guava easyCyte cytometer (Millipore) ...
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bioRxiv - Immunology 2023Quote: ... 7-Aminoactinomycin D (7-AAD) (Invitrogen) at 1:400 was used as a marker for dead cells ...
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bioRxiv - Neuroscience 2022Quote: BMEC-like cells were lysed with RIPA buffer (Thermo Fisher Scientific) supplemented with protease inhibitor (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: Neutrophil-like HL60 cells were maintained in RPMI media (Gibco 22400), supplemented with 10% heat-inactivated fetal bovine serum (hiFBS ...
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bioRxiv - Neuroscience 2023Quote: ... and Taqman probes for Dally-like protein (dlp) (Thermofisher Scientific Dm01798597_m1) and Gpdh1 (Thermofisher Scientific Dm01841185_m1) ...
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bioRxiv - Immunology 2023Quote: ... Foxp3+ Treg-like cells and iTregs were by TRIzol reagent (Invitrogen). The RNA library was generated using the NEB Ultra II Directional RNA Library Prep Kit and quantified using Qubit Bioanalyzer ...
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bioRxiv - Immunology 2023Quote: RAW264.7 macrophage-like cell line was cultured in RPMI medium (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2020Quote: ... Meso Scale Discovery (MSD)) were coated with mouse anti-human IgG antibody (CH2 domain, cat# MA5-16929, ThermoFisher Scientific) at 2 μg/mL in 1X PBS (50μL/well) ...
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bioRxiv - Neuroscience 2022Quote: ... using a commercial human fetal brain cDNA library containing cDNAs fused to the gal4 activation domain of pEXP-AD502 (ProQuest™, ThermoFisher Scientific, USA), as prey ...
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bioRxiv - Microbiology 2020Quote: ... The ectodomain of SADS-CoV spike protein without the transmembrane anchor and intracellular tail (residues 18-1068) was expressed by the Bac-to-Bac insect cell system (Invitrogen). To get the trimer ectodomain protein ...
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bioRxiv - Neuroscience 2023Quote: ... the hiPSCs were maintained for 7 days in a medium containing Neurobasal (Thermo Fisher Scientific, 12348017) and 1% neural induction supplement (NIS – Life Technologies ...
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bioRxiv - Biophysics 2023Quote: ... 150 mM NaCl) containing N-[4-(7-diethylamino-4-methyl-3-coumarinyl)phenyl]maleimide (CPM; Invitrogen) at a final concentration of 10 μM and incubated for 30 min in the dark on ice ...
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bioRxiv - Neuroscience 2021Quote: ... 7) anti-GFAP antibody (#14-9892-82, Fisher Scientific, Hampton, NH, USA) for an astrocyte biomarker ...
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bioRxiv - Biochemistry 2020Quote: ... different domains or subunits of the Spike protein and the extracellular domain of ACE2 were produced in Expi293F cells (Thermo Fisher Scientific). Expi293F cells were cultured at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... and coiled-coil domains through an LR reaction (ThermoFisher) into the moss expression construct ...
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bioRxiv - Cancer Biology 2022Quote: ... All primary glioblastoma stem cells-like were grown Neurobasal medium (Life Technologies) containing B27 supplement (Life Technologies) ...
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bioRxiv - Cell Biology 2019Quote: ... Human insulin-like growth factor 1 (IGF-1) was from Thermo Fisher and used as IGF-1 receptor activator.
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bioRxiv - Neuroscience 2020Quote: ... Neuron like differentiation was induced by switching the medium to Neurobasal (Gibco) supplemented with 2% L-Glutamine (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: For experiments using Human Plasma-Like Medium (HPLM – Gibco, ThermoFisher Scientific, Inc.), HPLM was supplemented with 10% dialysed FBS (dFBS ...
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bioRxiv - Cancer Biology 2022Quote: For experiments using Human Plasma-Like Medium (HPLM – Gibco, ThermoFisher Scientific, Inc.), HPLM was supplemented with 10% dialysed FBS (dFBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Alternatively cells were cultured in Human Plasma-like Medium (HPLM, Gibco, #A4899101) supplemented with H14 media additives.52 Cells were regularly screened for absence of mycoplasma contamination with the ATCC Universal Mycoplasma Detection Kit (ATCC ...
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bioRxiv - Cell Biology 2023Quote: ... Qi33,65–67 and was used since it can recognize SEL1L without its FN2 domain) was applied at 1:100 dilution followed by secondary antibody conjugated to Alexa Fluor 488 or 594 (Invitrogen) at 1:100 ...
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bioRxiv - Cancer Biology 2019Quote: Total mitochondrial mass and mitochondrial transmembrane potential (ΔΨm) were analyzed by incubating cells with 50 nM MitoTracker Green-FM or 200 nM TMRM (Thermo-Fisher Scientific), respectively ...
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bioRxiv - Immunology 2020Quote: ... An expression plasmid expressing the same pre-fusion stabilized full length transmembrane protein used the pcDNA3.1 backbone and was directly synthesized and cloned into the vector by GeneArt (Thermo Fisher Scientific). A plasmid that expressed a soluble pre-fusion version was directly synthesized and cloned into the pcDNA3.1 backbone vector by GeneArt (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... and the retinal explants with the transmembrane were embedded in a 1:1 mixture of 40% sucrose in PBS: OCT compound (Fisher Scientific) in liquid nitrogen for cryosection ...
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bioRxiv - Cell Biology 2022Quote: ... and 7-AAD (7-Aminoactinomycin D) (Thermofisher, # A1310) was used for DNA staining ...