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Citations for Thermo Fisher :
9801 - 9850 of 10000+ citations for Mouse Anti Human Ig kappa chain Alexa Fluor 647 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: TE proteins were fluorescently labeled with the amine-reactive dye Alexa Fluor 647 NHS-Ester (Thermo Fisher Scientific, Rockford, IL, USA) as follows ...
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bioRxiv - Physiology 2020Quote: ... samples were washed in PBS for 5 minutes and then stained with α-Bungarotoxin-Alexa Fluor™ 647 conjugate (Invitrogen, UK) diluted 1:500 in PBS for at least 30 minutes in a dark environment at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... DTT was removed and the sybody was re-buffered to degassed PBS using a PD10 desalting column and immediately mixing the sybody with Alexa Fluor 647 C2 maleimide (ThermoFisher Scientific) at a molar ratio of 1:3.6 ...
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bioRxiv - Immunology 2021Quote: ... neurons were incubated for one hour in Alexa Fluor® 488- and 647-conjugated secondary antibodies for multi-color imaging (Invitrogen). Nuclei were stained with Hoechst 33258 (Life Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... for 1 hour or immediately processed for EdU staining performed according to directions provided with the Click-iT Edu Alexa Fluor 647 Imaging Kit (Invitrogen C10340). For alkaline phosphatase staining ...
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bioRxiv - Molecular Biology 2019Quote: ... The tissues were counterstained with wheat germ agglutinin (WGA) tagged with Alexa Fluor™ 647 (#A-31573, ThermoFisher Scientific, Waltham, MA) and DAPI (#H-1500 ...
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bioRxiv - Genetics 2020Quote: ... The larvae were sequentially washed in PBSTx and incubated with Alexa Fluor 647 Phalloidin (1:100) (Thermo Fisher Scientific, CA, USA) in PBSTw (1% PBS ...
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bioRxiv - Biochemistry 2021Quote: S9C:WT (1:2) αSyn amyloid fibres were incubated with Alexa Fluor 647 maleimide dye (10-fold molar excess, Thermo Fisher Scientific) and Biotin-X ...
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bioRxiv - Developmental Biology 2021Quote: ... The Click-iT Plus OP-Puro Alexa Fluor 647 assay was done according to the manufacturer’s protocol (Click-iT Cell Reaction Buffer Kit, ThermoFisher Scientific, C10269). Cells were resuspended in 200 μL PBS supplemented with 3% FBS and 0.1% saponin and analyzed by flow cytometry ...
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bioRxiv - Neuroscience 2020Quote: ... Cell proliferation was detected through use of the Click-iTTM EdU Cell Proliferation Kit for Imaging (Alexa Fluor 647 dye, ThermoFisher Scientific). Methoxy-X04 was used to counterstain amyloid deposits (1 mg/ml MeO-X04 (5 mg/ml in 10% DMSO ...
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bioRxiv - Immunology 2020Quote: ... EDU incorporation was determined using the Click-ITTM Plus EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific), according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... the antibody produced in-house from HPC-4 hybridoma, ATCC HB-9892, and labeled with Alexa Fluor 647 NHS Ester, Thermo Fisher), showing superior Wnt display on the hFzd5CRD-1TM cells ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then fixed in 4% PFA for 10 min at room temperature and stained with the Click-iT EdU Alexa Fluor 647 assay kit (Life Technologies) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... newly synthesized DNA was labeled using the Click-iT Plus 5-ethynyl-2’-deoxyuridine (EdU) Alexa Fluor 647 flow cytometry assay kit (ThermoFisher Scientific) according to manufacturer ‘s instructions ...
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bioRxiv - Immunology 2022Quote: ... The array was immersed in phenol red-free cell-culture media containing a dilution of 1:60 Annexin V Alexa Fluor 647 (AF647) (Life Technologies) to detect cell apoptosis ...
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bioRxiv - Immunology 2022Quote: ... Fluorescently-labeled mMOGtag directly conjugated to Alexa Fluor 647 was generated in house using the NHS Ester fluorophore labeling kit (Life Technologies) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... the retrograde tracer Cholera Toxin Subunit B conjugated with Alexa Fluor 555 or 674 (CTB 555 or CTB 647, 1 mg/ml, Invitrogen, USA) was bilaterally injected with a micropipette connected to Nanoject III microinjector (Drummond Scientific ...
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bioRxiv - Molecular Biology 2022Quote: Cell pellets were re-suspended in 240 µl PBS and 9 μl 10% triton X-100 containing 0.3 µl of 1 mg/ml Streptavidin conjugated with Alexa Fluor® 647 (Life technologies) and 0.6 μl of 1 mg/ml Wheat Germ Agglutinin (WGA ...
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bioRxiv - Cell Biology 2022Quote: ... secondary antibodies raised in donkeys or goats labelled with Alexa Fluor® 647 were used (1:500, Jackson ImmunoResearch and Invitrogen). Following acquisition of the antibody signal ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were then washed three times with PBS and treated with the secondary antibody conjugated with Alexa Fluor 647 (A-21245, Thermo Fisher), which was 1,000 times diluted in the blocking buffer for 1 h at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Afterwards slices were repeatedly washed and incubated overnight at RT in PBS containing a mixture of Alexa Fluor 647-conjugated streptavidin (1:500, S21374, Invitrogen, UK) and secondary antibodies (donkey anti-goat Alexa Fluor Plus 488 ...
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bioRxiv - Cell Biology 2024Quote: ... Detection of EdU was accomplished using the Click-iT Plus EdU Alexa Fluor 488 or 647 Imaging Kit (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... according to the manufacturer’s protocol from Alexa Fluor®647 Labeling Kit (Microscale Protein Labeling Kit, cat. no. A30009; ThermoFisher Scientific) and stored at −20°C ...
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bioRxiv - Neuroscience 2023Quote: ... Three 5 min washes with PBS preceded a 2-hour incubation at room temperature with 4 μg/mL Alexa Fluor 647-conjugated secondary antibody (ThermoFisher A21244) prepared in blocking buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... The EdU incorporation was determined with the Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay kit (C10424; Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Fluorescent labelling of proteins of LEVs and SEVs was conducted as follows: pellets were resuspended in 50 μl PBS containing 10 µM Alexa Fluor 647 NHS Ester (Life Technologies) and incubated for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... and the resulting EdU signals were detected with Alexa Fluor 647 using a Click-iT EdU Imaging Kit (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2022Quote: ... The Alexa-647 labelling for detection used in SMC and in ELISA assays was performed using the Alexa Fluor-647 Monoclonal Antibody Labeling Kit from Thermo Fisher Scientific (catalog #A20186 ...
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bioRxiv - Immunology 2023Quote: ... The 14.4.4 mAb and the I-Ak-reactive 11-5.2 mAb were randomly labeled via available lysine residues with Alexa Fluor 647 carboxylic acid succinimidyl ester (Thermo Fisher Scientific) according to the manufacturer’s instructions and purified via gel filtration (Superdex-200 10/300 GL ...
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bioRxiv - Molecular Biology 2023Quote: ... then washed 4× 5min in PBS with subsequent incubation with secondary antibodies (Alexa-Fluor 488, 555, 568 or 647 (Molecular Probes or Invitrogen)) ...
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bioRxiv - Genomics 2023Quote: ... that can bind to the readout sequences on the primary probes or primary antibodies were conjugated in-house to Alexa Fluor 647–NHS ester (Invitrogen A20006), Cy3B–NHS ester (GE Healthcare PA63101) ...
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bioRxiv - Neuroscience 2023Quote: A 10µL Hamilton syringe (QSI Stereotax Injector) containing 0.25 µL of the retrograde tracer Cholera Toxin Subunit B – Alexa Fluor 647 (CTB) (Thermofisher Scientific, #C34778) was fitted with a fine tipped glass capillary tube and lowered into the right adBNST (+0.25 –+ 0.35 mm AP ...
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bioRxiv - Cancer Biology 2023Quote: ... F-actin was stained by incubating blocked slides for 2 hours at RT with Alexa Fluor 647 Phalloidin (1:400, Invitrogen #A22287).
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bioRxiv - Neuroscience 2023Quote: ... injecting twice on opposite points with 0.5µl of CTB-subunit conjugated with Alexa Fluor 488 or 647 (Invitrogen, C34775/C22843/C34778) as described in (Rebsam et al. ...
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bioRxiv - Cell Biology 2023Quote: ... the glasses were incubated for 2 h at room temperature with secondary antibodies (1:800 Alexa Fluor 594 and 647 antibodies from Molecular Probes) and mounted in polyvinyl alcohol mounting medium with DAB-CO [52] ...
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bioRxiv - Molecular Biology 2023Quote: Cell pellets were re-suspended in 240 µl PBS and 9 μl 10% triton X-100 containing 0.3 µl of 1 mg/ml Streptavidin conjugated with Alexa Fluor® 647 (Life technologies) and 0.6 μl of 1 mg/ml Wheat Germ Agglutinin (WGA ...
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bioRxiv - Cell Biology 2023Quote: ... A reference sample was prepared by pooling equal amounts (by total protein) of all samples and labeling with Alexa Fluor 647-NHS (Thermo Fisher). Table S1 summarizes the print list for the experiment Printing ...
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bioRxiv - Physiology 2023Quote: ... and MHC IIb sections were incubated for 1 hr with the following secondary antibodies: Alexa Fluor 647 (1:250; Invitrogen, A21242), Alexa Fluor 488 (1:500 ...
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bioRxiv - Immunology 2023Quote: ... was added to the culture media for 24 h and visualization was performed according to the manufacturer’s instruction with Click-iT EdU Cell Proliferation Kit Alexa Fluor 647 (ThermoFisher, Waltham, MA). Organoids were imaged on a Leica SP8 STED 3X confocal microscope with a 40X objective ...
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bioRxiv - Genetics 2023Quote: To measure proliferating phNPCs we measured incorporation of 5-ethynyl-2’-deoxyuridine (EdU) into newly synthesized DNA using a Click-iT EdU Imaging Kit with Alexa Fluor 647 (Invitrogen C10340). At 8 days post transduction ...
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bioRxiv - Developmental Biology 2022Quote: ... The incorporation OPP was revealed using a Click-iT™ OPP Alexa Fluor™ 647 imaging kit (cat. no. C10458, Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... the Click reaction was performed using the Click-iT™ Plus OPP Alexa Fluor 647 Protein Synthesis Assay Kit (ThermoFisher, C10458) at RT for 30 mins ...
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bioRxiv - Cell Biology 2023Quote: The endocytic uptake was monitored in mPTCs cells following incubation for 60 min at 4 °C with 50μg × ml−1bovine serum albumin (BSA)–Alexa−Fluor−647 (A34785, Thermo Fisher Scientific) in complete HEPES-buffered Dulbecco’s modified Eagle’s medium ...
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bioRxiv - Bioengineering 2023Quote: ... The beads were then washed with PBSB and incubated with a 1:1000 dilution of streptavidin Alexa Fluor 647 (Invitrogen, S32357) and 1:1000 dilution of goat anti-human Fc F(‘ab)2 Alexa Fluor 488 (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2023Quote: ... PDMS stamps were incubated with solution containing 30 μg/ml fibronectin and 2 μg/ml fibrinogen Alexa Fluor 647 conjugate solution (F35200, Invitrogen, USA) in Dulbecco’s phosphate-buffered saline (Sigma-Aldrich ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... Coverslips were washed three times in PBS and incubated for 1hr at room temperature and in the dark with Alexa Fluor (488, 568 or 647)-conjugated secondary antibodies (Life Technologies) diluted in PBS/5% BSA ...
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bioRxiv - Immunology 2023Quote: ... EdU was detected using the Click-iT reaction coupled to an Alexa Fluor 647 azide following the instructions of the manufacturer (Invitrogen, #C10086). Intracellular flow analyses were performed on BD FACSymphony A3 Cell Analyzer (BD Biosciences).
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bioRxiv - Cancer Biology 2023Quote: ... The tops of the micropillars were coated with a mixture of unlabeled and Alexa Fluor 647-labeled fibronectin (1:5, Life Technologies) using micro-contact printing ...
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bioRxiv - Developmental Biology 2023Quote: EdU detection was conducted with (Click-iT™ EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 647 dye, Invitrogen, C10340) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 100-300 µg protein were used for copper-catalyzed cycloaddition reactions using the Click-iT Plus Alexa Fluor 647 Picolyl Azide Toolkit (Fisher Scientific). Proteins were separated using NuPAGE 10% Bis-Tris gels and gels were fixed with 10% acetic acid ...