Labshake search
Citations for Thermo Fisher :
9751 - 9800 of 10000+ citations for Mouse Anti Human Ig kappa chain Alexa Fluor 647 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... then washed with PBST and incubated with goat anti-mouse IgG (H+L) Alexa Fluor 594 (1:500 dilution by the blocking solution, A-11032, Thermo Fisher Scientific) at room temperature for at least 2 hours ...
-
bioRxiv - Genetics 2023Quote: ... The following secondary antibodies and dilutions were used: goat anti-mouse IgG(H+L) (Alexa Fluor 594, ThermoFisher, A-11005, 1:1000), and goat anti-rabbit IgG(H+L ...
-
bioRxiv - Cell Biology 2023Quote: ... The cover slips were then incubated with 150 µL 1:500 secondary antibody (Alexa Fluor 488-conjugated Goat anti-mouse IgG, Thermo Fisher Scientific) diluted in blocking solution in the dark at RT for 120 min ...
-
bioRxiv - Cell Biology 2023Quote: ... The samples were again washed three times in PBST and then incubated in either Alexa Fluor 555 anti-mouse (A-31570, Thermo Fisher Scientific) or Alexa Fluor 488 anti-rabbit (711-545-152 ...
-
bioRxiv - Molecular Biology 2023Quote: ... brain slices were washed 3X in 1X TBS and then incubated for 2 hours at RT with a fluorescent-tagged Alexa Fluor 568 anti-mouse secondary antibody (1:500; Life Technologies A11004). Brain sections were then washed 3X in 1X TBS ...
-
bioRxiv - Neuroscience 2023Quote: ... Their respective secondary antibodies were Dylight 405 donkey anti-rabbit igG H+L (Jackson Immuno Research #711-475-152, Alexa Fluor 488 donkey anti-mouse IgG H+L (Life Technologies #A21202), Alexa Fluor donkey anti-guinea-pig IgG H+L (Jackson ImmunoResearch #706-605-148 ...
-
bioRxiv - Bioengineering 2024Quote: ... the cells washed three times in ice-cold PBSA were incubated with goat anti-mouse Alexa Fluor 488 antibody (Invitrogen, 2mg/mL) and anti-His6x monoclonal antibody conjugated with Alexa Fluor 647 (Invitrogen ...
-
bioRxiv - Microbiology 2024Quote: ... incubated 1 h at room temperature with DAPI and conjugated secondary antibodies: anti-mouse IgG Alexa Fluor 488 (Life Technologies, 1:1000); anti-mouse IgG Alexa Fluor 555 (Life Technologies ...
-
bioRxiv - Immunology 2024Quote: ... The plates were washed with 2% FBS in PBS and stained with Goat anti-mouse IgG/IgM Alexa Fluor 488 conjugated secondary antibody (Invitrogen A-10680) diluted in 2% FBS in PBS (1:300 ...
-
bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies used were: (1) Alexa Fluor Plus 488 Goat anti-Mouse IgG (H+L) (for PV stain; catalog # A32723, Invitrogen; RRID: AB_2633275), (2 ...
-
bioRxiv - Genomics 2024Quote: ... Membranes were washed 3 times in TBS-Tween before incubation with a secondary antibody anti-mouse IgG Alexa Fluor 790 nm (Invitrogen Cat #A11375), anti-goat IgG Alexa Fluor 800 nm (Invitrogen Cat# A32930 ...
-
bioRxiv - Cell Biology 2024Quote: ... devices were washed twice with PBS and then incubated for 2 h with Alexa Fluor Goat anti-mouse 488 (1:250, Invitrogen, A-11029) secondary antibody and Hoechst (5 μg/mL ...
-
bioRxiv - Immunology 2024Quote: ... wells were washed three times with SIFA buffer and secondary antibody Alexa Fluor® 488 Goat Anti-Mouse IgG (H+L) (Invitrogen#A11001) diluted 1/200 added for a 1-hour incubation step on ice ...
-
bioRxiv - Biophysics 2021Quote: ... solution (pH 8) and incubated in 0.1M sodium bicarbonate solution (pH 8) with 2 uM Alexa Fluor™ 647 NHS Ester (Invitrogen, A37573) for 30 minutes ...
-
bioRxiv - Cell Biology 2020Quote: ... The cells were gently washed three times in PBS and the plasma membrane labelled with Wheat Germ Agglutinin-Alexa Fluor 647 (Molecular Probes) at 5µg/ml for 10mins in PBS ...
-
bioRxiv - Cell Biology 2020Quote: ... EdU-pulsed cells were processed according to the manual of the Click-iT™ EdU Alexa Fluor 647 imaging kit (Invitrogen). Blocking was performed using 10% goat serum (Dako ...
-
bioRxiv - Immunology 2021Quote: ... the samples were rinsed three times and stained for PD-1/PD-L1 proteins using an Alexa Fluor 647 Tyramide SuperBoost kit (#B40926, ThermoFisher Scientific). Firstly ...
-
bioRxiv - Biophysics 2020Quote: ... at a 1.5 fold molar surplus and then incubated for 2 hours followed by an incubation with 1.5-fold excess Alexa Fluor 647 succinimidyl ester (Thermo Fisher Scientific) in the presence of 330 mM NaHCO3 for 2 hours at room temperature ...
-
bioRxiv - Developmental Biology 2020Quote: ... The animals were then dissected and germlines processed using the Click-iT Plus EdU Alexa Fluor 647 Imaging Kit (ThermoFisher Scientific) per the manufacturer’s recommendations ...
-
bioRxiv - Cell Biology 2022Quote: ... TUNEL assay was carried out using the Click-iT Plus TUNEL Assay for in situ apoptosis detection with Alexa Fluor 647 dyes (ThermoFisher Scientific). Pictures were taken using a Leica DM6 microscope.
-
bioRxiv - Cancer Biology 2019Quote: ... Cells were incubated for 30 min at room temperature in Click-IT reaction (100mM Tris pH 8.5, 10 nM Alexa Fluor 647-azide (Cat# A10277, Thermo Fisher Scientific), 1 mM CuSO4 ...
-
bioRxiv - Biophysics 2020Quote: Water soluble dye and conjugated proteins were prepared according to the manufactures instructions and added to tGPMV and GPMV solution after isolation from the cell culture in the following final concentrations: Alexa Fluor 647 NHS-Ester (Thermo Fisher) at 400 μM ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cell cycle analysis was performed using the Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (ThermoFisher Scientific) according to manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2020Quote: ... The homogenized samples were then labeled with Alexa Fluor 555-NHS as previously described.26 Reference sample was prepared by mixing equal amounts (by total protein) of all samples and labeling with Alexa Fluor 647-NHS (Thermo Fisher). Supplementary Table 1 summarizes the print list for each experiment ...
-
bioRxiv - Bioengineering 2020Quote: Beads are 3 µm radius biotin-polystyrene microspheres (Kisker Biotech,PC-B-3.0) on which we attached Alexa Fluor (AF) 647 functionalized with streptavidin (Life Technologies, S21374). We prepared a solution containing 500 µL of water ...
-
bioRxiv - Cell Biology 2021Quote: ... and the resulting EdU signals were detected with Alexa Fluor 647 using a Click-iT EdU Imaging Kit (Thermo Fisher Scientific) (29) ...
-
bioRxiv - Cancer Biology 2020Quote: A549/KZFP cells and NC cells were stained with Annexin V conjugated to Alexa Fluor™ 647 (Invitrogen Carlsbad, CA; #S32357). After staining ...
-
bioRxiv - Physiology 2021Quote: ... Slides were washed with blocking solution and incubated with appropriate secondary antisera (donkey or goat Alexa Fluor® 488, 546, 555, 633 or 647; Invitrogen) diluted 1:400 and Hoechst diluted 1:1500 for 1 hr ...
-
bioRxiv - Biochemistry 2021Quote: ... Secondary fluorophore-coupled antibodies were all from the Alexa Fluor range (568 ref. A10042 and 647 ref. A21469, IFA 1:1000, Life Technologies).
-
bioRxiv - Microbiology 2020Quote: ... or rabbit anti-IFITM3 (Cat. nr. AP1153a, Abcepta, 1:500) and secondary antibodies conjugated to Alexa Fluor® 488 and 647 (1:1000, Invitrogen) for one hour at room temperature ...
-
bioRxiv - Systems Biology 2020Quote: ... The 5’ amine-modified DNA oligonucleotides (Integrated DNA Technologies) with the readout probe sequences were conjugated to Alexa Fluor 647-NHS ester (Invitrogen A20006) or Cy3B-NHS ester (GE Healthcare PA63101 ...
-
bioRxiv - Neuroscience 2020Quote: ... Small craniotomies were made at these sites and cholera toxin subunit B (CTB) conjugated with Alexa Fluor (CTB-488, −555, −647, Thermo Fisher) was injected using glass micropipettes ...
-
bioRxiv - Systems Biology 2021Quote: ... Integrated DNA Technologies) of 12-15-nt in length were designed and conjugated to Alexa Fluor 647-NHS ester (Invitrogen A20006) or Cy3B-NHS ester (GE Healthcare PA63101 ...
-
bioRxiv - Biophysics 2020Quote: ... Protein A IgG binding buffer (21001) and Alexa Fluor(tm) 647 NHS Ester (Succinimidyl Ester; A20006) were purchased from Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... ParBF protein was buffer exchanged into gel filtration buffer without reducing agent and incubated with a two-fold molar excess of Alexa Fluor 647 C2 Maleimide (Thermo Fisher) for 30 min at room temperature ...
-
bioRxiv - Bioengineering 2022Quote: ... sections were processed according to the manufacturer’s instructions (Click-iT™ EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 647, Invitrogen). Histological images were taken with an Axioskop 2 Plus upright microscope (Zeiss ...
-
bioRxiv - Neuroscience 2020Quote: ... was added to 1 mg (1.25 μmol, MW ~ 800) of the Alexa-Fluor®-647 carboxylic acid succinimidyl ester (Invitrogen/ThermoFisher Scientific). The reaction mixture was stirred for 5 h at room temperature in the dark ...
-
bioRxiv - Biochemistry 2020Quote: ... 1*10^8 yeast were pelleted and resuspended in 500 µl PBM containing 100 nM Streptavidin-Alexa Fluor 647 conjugate (Thermo Fisher) and 400 nM PLpro S1*-mutant and incubated for 40 min at 4 °C ...
-
bioRxiv - Biophysics 2021Quote: ... cells were washed 3x with PBS buffer containing 1% BSA and incubated with the secondary antibody (Alexa Fluor 647, ThermoFisher Scientific) diluted 1/1000 in DPBS buffer containing 1% BSA for 45 min at 37°C ...
-
bioRxiv - Cancer Biology 2021Quote: Cell cycle was assessed using Click-iT™ Plus EdU Alexa Fluor™ 647 Flow Cytometry Assay kit (Invitrogen, ThermoFisher Scientific). Briefly ...
-
bioRxiv - Cancer Biology 2021Quote: Cell cycle was assessed using Click-iT™ Plus EdU Alexa Fluor™ 647 Flow Cytometry Assay kit (Invitrogen, ThermoFisher Scientific). Briefly ...
-
bioRxiv - Developmental Biology 2022Quote: ... permeabilized with 0.1% Triton for 20 minutes at room temperature and labeled with Alexa Fluor 647 using the Click-iT reaction (Thermo Fisher) for 30 minutes according to manufacturer instructions ...
-
bioRxiv - Genetics 2022Quote: ... and EdU labeled cells were stained using a Click-IT reaction with Alexa Fluor 647 nm-azide (Click-iT EdU Imaging Kit, Invitrogen, CA).
-
bioRxiv - Cell Biology 2022Quote: ... the cells were washed with HBSS and exposed to 0.5 μg/ml Transferrin-Alexa Fluor 647 (A647-Tf, Thermo Fisher Scientific) dissolved in complete DMEM for 10 minutes at 37°C ...
-
bioRxiv - Biophysics 2022Quote: Biotinylated hLRP6E1E2 was tetramerized with Alexa 647 labeled streptavidin (SA647, streptavidin with a cysteine residue produced in house, and labeled with Alexa Fluor 647 C2 Maleimide, Thermo Fisher) and cells were stained with 30-200 nM hLRP6E1E2-SA647 tetramer (120-800 nM hLRP6E1E2 as monomer) ...
-
bioRxiv - Cancer Biology 2022Quote: ... permeabilized and stained for EdU (Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit, cat#C10635 from ThermoFisher). After the staining ...
-
bioRxiv - Developmental Biology 2019Quote: ... 5-ethynyl-2’-deoxyuridine (EdU) labeling was performed using the Click-iT EdU Alexa Fluor 647 Imaging Kit (Invitrogen Cat#C10340) following manufacturer instructions ...
-
bioRxiv - Cell Biology 2019Quote: ... The subsequent steps for EdU staining carried out as indicated in the protocol provided with the Click-iT EdU Alexa Fluor 647 Imaging Kit (ThermoFisher Scientific).
-
bioRxiv - Immunology 2019Quote: ... purified Sema3A protein was labelled with AF647 using Alexa Fluor 647 Antibody Labeling Kit according to protocol (Invitrogen, cat. no A20186) at a F/P rate at 1-2.
-
bioRxiv - Cell Biology 2019Quote: ... was incubated with cells for 30 min at room temperature prior to staining with the appropriate Alexa Fluor 647 secondary antibody (Life Technologies) for 30 min on ice ...