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Citations for Thermo Fisher :
9701 - 9750 of 10000+ citations for Mouse Anti Human Ig kappa chain Alexa Fluor 647 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The cells were washed twice with FACS buffer and then incubated with 50 μL of 4 μg/ml goat anti-mouse IgG-Alexa Fluor 488 (Thermo Fisher Scientific) in FACS buffer for 30 min on ice ...
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bioRxiv - Microbiology 2020Quote: ... Cells infected with rSARS-CoV-2-Venus or -mCherry were washed with PBS and stained with either Alexa Fluor 594 goat anti-mouse IgG (Invitrogen; 1:1000) or fluorescein isothiocynate (FITC)-conjugated goat anti-mouse IgG (Dako ...
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bioRxiv - Microbiology 2021Quote: ... the cells were washed two times and resuspended in FACS buffer containing the Alexa Fluor-488-labeled secondary antibodies at a 1:200 dilution: goat anti-mouse (Invitrogen cat. A11001), goat anti-rabbit (Invitrogen cat ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed with PBS and incubated for 2 hours at RT with Alexa Fluor 555 donkey anti-mouse (1:1000, Life Technologies-Invitrogen). Cells were finally stained with DAPI (1:5000 in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated for 1 hr at room temperature with fluorophore-conjugated secondary antibodies: anti-mouse Alexa Fluor 594 (1:200, A-11005, Invitrogen, Thermo Fisher Scientific), anti-rabbit Alexa Fluor 488 (1:200 ...
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bioRxiv - Microbiology 2019Quote: ... The coverslips were then washed three times with PBS and incubated with saponin buffer containing respective fluorophore-conjugated secondary antibodies: goat anti-mouse Alexa Fluor 488 (Thermo Fisher A11029) and anti-rabbit Alexa Fluor 647 (Cell Signaling #4414 ...
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bioRxiv - Cell Biology 2019Quote: ... Binding of the primary antibody was detected by incubation with Alexa Fluor 488-conjugated goat anti-mouse or secondary antibody (Invitrogen Molecular Probes). The sexual stages were highlighted by double-labelling with rabbit antibodies directed against Pfs230 ...
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bioRxiv - Cell Biology 2019Quote: ... Secondary antibodies were 1:500 dilutions of Alexa Fluor 488 and 568 anti-mouse IgG (Molecular Probes, A-11001 and A-11004) and Alexa Fluor 488 and 568 anti-rabbit IgG (Molecular Probes ...
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bioRxiv - Biophysics 2020Quote: ... The membrane was then incubated for 2 h at room temperature in PBS supplemented with 0.05% tween and an anti-mouse-Alexa Fluor 594 secondary antibody conjugate (ThermoFisher Scientific, Waltham, USA) at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... To visualize antibody binding the following secondary antibodies were used: goat anti-mouse Alexa Fluor 488 (2 μg/ml, catalog number A11029, Thermo Fisher Scientific) and goat anti-rabbit Alexa Fluor 546 (2 μg/ml catalog number A11035 ...
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bioRxiv - Biochemistry 2021Quote: ... cells were incubated for 1 hour at room temperature with the following secondary antibodies: Goat anti-mouse Alexa Fluor 488 (Invitrogen A A11001), donkey anti-rabbit Rhodamine Red (Jackson Immuno Research 711-295-152) ...
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bioRxiv - Cell Biology 2021Quote: ... but the immunolabeling was carried out with mouse monoclonal anti-LAMP-1 antibody conjugated to Alexa Fluor 488 (Thermo Fisher Scientific; RRID:AB_2016657) or with corresponding isotype control (RRID:AB_470230).
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then spun down and resuspended in 140 µg/mL goat-anti mouse Alexa Fluor 488 (# A-11008, Thermo Fisher Scientific) in 0.5% Tween 20 in PBS / 5% BSA for 30 mins ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed twice with PBS and stained with 1:250 dilution of Alexa fluor 488-conjugated goat anti-mouse IgG (Thermo Fisher, UK), incubated for 20 minutes at 4°C in the dark ...
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bioRxiv - Molecular Biology 2021Quote: ... The signals were visualized using the following fluorochrome-coupled secondary antibodies: Alexa fluor 568 goat anti-mouse IgG1a (Invitrogen, cat# A-21124), Alexa fluor 488 goat anti-rabbit IgG (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... cells were washed thrice with 1X PBS-T and incubated with anti-mouse Alexa Fluor 594 conjugated secondary antibody (Invitrogen, Carlsbad, CA) for 1 h at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... and with Alexa Fluor 488- (in green) or 633- (in red) conjugated goat anti-mouse or - rabbit IgG as secondary Abs (Invitrogen, Carlsbad, CA), and counterstained with 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2022Quote: ... For KV2.2 channels, NDS was substituted for normal goat serum (NGS, 3%) and Alexa Fluor® 555 goat anti-mouse IgG1 (Thermo Fisher Scientific Cat# A-21127 ...
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bioRxiv - Molecular Biology 2023Quote: ... The primary antibody was then detected by an Alexa Fluor™ 488-conjugated goat anti-mouse secondary antibody (Invitrogen, Cat# A-11001), which was diluted 1:500 in blocking solution (PBS containing 1% FCS [Bioconcept ...
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bioRxiv - Physiology 2022Quote: ... 2,7-Bis(2-carboxyethyl)-5-carboxyfluorescein acetoxymethyl ester (BCECF-AM) and Alexa Fluor 488 and 568–conjugated goat anti-mouse and antirabbit secondary antibodies were from Invitrogen (Carlsbad, CA). Mouse monoclonal antibodies to the HA epitope were from Covance Research Products (Princeton ...
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bioRxiv - Molecular Biology 2022Quote: ... and once with PBS followed by incubation with monoclonal Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (dilution 1:1000; Molecular Probes, USA) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... The binding of PfPHB1 and PfPHB2 primary antibodies was detected by incubating the samples with monoclonal Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (dilution 1:1000; Molecular Probes, USA) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Drexel University) at 1:500 followed by goat anti-mouse IgG H+L Alexa Fluor™Plus 488 (Invitrogen, A32723, Lot # SG251135) at 1:500 and goat anti-rabbit IgG H+L AlexaFluor™ 568 (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... The primary antibody was then revealed by incubation for 30 min at 37 °C in the dark with donkey anti-mouse Alexa Fluor-488 (1:250; Thermo Fisher Scientific) diluted in Triton X-100 (0.3% ...
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bioRxiv - Microbiology 2024Quote: ... cells were washed with DMEM and subsequently incubated with either Alexa Fluor 594-conjugated goat anti-mouse IgG (Thermo Fisher Scientific; A32742) or a combination of Alexa Fluor 488-conjugated goat anti-human IgG (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... samples were incubated in a goat anti-mouse secondary antibody conjugated to Alexa Fluor Plus 488 (Invitrogen, Cat# A-11029, 1:1000) at room temperature for 1 h ...
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bioRxiv - Immunology 2024Quote: ... The conjugated antibody used in this study was mouse anti-GFAP Alexa Fluor 488 (GA5) (Fisher Scientific, 1:100, #53-9892-82). For FluoroMyelin
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bioRxiv - Immunology 2024Quote: ... The conjugated antibody used in this study was mouse anti-GFAP Alexa Fluor 488 (GA5) (Fisher Scientific, 1:100, #53-9892-82). Human brain tissue was obtained from patients diagnosed with clinical and neuropathological MS diagnosis according to the revised 2017 McDonald’s criteria60 ...
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bioRxiv - Cell Biology 2024Quote: ... The following secondary antibodies were used for immunofluorescence or U-ExM: Alexa Fluor conjugated donkey anti-mouse A488 (#A21202; Thermo Fisher Scientific), donkey anti-rat A488 (#A21208 ...
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bioRxiv - Microbiology 2024Quote: ... The cells were washed 3 times with 1X PBS and incubated with goat anti-mouse IgG (H&L)-Alexa Fluor 488 (1:1000 dilution, Thermo Fisher Scientific) at 37°C for 2h ...
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bioRxiv - Cancer Biology 2024Quote: ... and neurons were incubated at RT for 45 min (PMSNs) or 48h (DRGs) in secondary goat antibodies: anti-mouse Alexa Fluor 405 (1:400, Invitrogen, A-31553), anti-chicken Alexa Fluor 488 (1:400 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then washed before the addition of Alexa Fluor 488-conjugated goat anti-mouse IgG (1 µg/ml dilution; 30 min; Invitrogen, Eugene, OR). Fixed and labeled cells were mounted onto slides before visualization with a Leica sp8 confocal laser scanning microscope and analysis with LASX software ...
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bioRxiv - Cancer Biology 2022Quote: ... coverslips were inverted onto 100 μl of antibody block with secondary antibodies (Alexa Fluor 488 anti-mouse, 1:200, A11029; Thermo Fisher Scientific) and DAPI (DNA ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibodies used were Alexa Fluor 633-conjugated goat anti-mouse antibody (Cat No. A-21050, 1:200, Thermo Fisher Scientific) and Alexa Fluor 488-conjugated goat anti-rabbit antibody (Cat No ...
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bioRxiv - Molecular Biology 2022Quote: ... the slides wash washed three times with 1×PBS and incubated for 30 min with secondary antibodies: Alexa Fluor 568 goat anti-mouse IgG (1: 100, Invitrogen/Molecular probes) and Alexa Fluor 488 goat anti-rat IgG (1 ...
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bioRxiv - Microbiology 2023Quote: ... The plates were washed with 2% FBS in PBS and stained with Goat anti-mouse IgG/IgM Alexa Fluor 488 conjugated secondary antibody (Invitrogen A-10680) diluted in 2% FBS in PBS (1:300 ...
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bioRxiv - Microbiology 2023Quote: ... The following secondary antibodies were used at a 1:2,000 dilution: donkey anti-mouse IgG Alexa Fluor Plus 555 conjugate (Thermo Fisher Scientific, A32773), donkey anti-rabbit IgG Alexa Fluor Plus 647 conjugate (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were washed five times in 0.1% Triton and incubated for 1 hour with fluorescent secondary antibody (Alexa Fluor 568 anti-Mouse (Invitrogen, #A11005, 1:500) diluted in the same buffer ...
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bioRxiv - Microbiology 2023Quote: ... All bacteria (both intact and disrupted vacuoles) were identified by goat anti-mouse IgG Alexa Fluor 488 (Invitrogen, Cat# A11001, 1:500). Images were taken using Zeiss observer Z1at 63X and individual BMDMs were scored for disruption based on staining with goat anti-mouse Alexa Fluor 594 (antibody accessible in the absence of methanol ...
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bioRxiv - Cell Biology 2023Quote: ... The following secondary antibodies were used: goat anti-mouse conjugated to Alexa Fluor 488 and 568 (1:400, Invitrogen A11001 and A11004) and goat anti-human conjugated to Alexa Fluor 647 (1:400 ...
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bioRxiv - Genomics 2023Quote: ... the samples were incubated for 3 h at room temperature in the presence of Alexa Fluor 546-conjugated goat anti-mouse IgG (1:1,000; Life Technologies, Carlsbad, CA). Nuclei were counterstained with 10 μg/mL Hoechst 33342 in PBST for 20 min ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed (three times in 1x PBS for 10 min at RT) and incubated with Alexa Fluor 488 goat anti-mouse secondary antibody (1:1000, Thermo Fisher Scientific), Alexa Fluor 647 goat anti-rabbit (1:500 ...
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bioRxiv - Biochemistry 2022Quote: ... secondary antibodies were diluted in blocking buffer as follows: Alexa Fluor 488-Goat anti-Mouse IgG (H+L) secondary antibody (1:1000; Life Technologies; A11029) an Alexa Fluor 568-Goat anti-Rabbit IgG (H+L ...
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bioRxiv - Cancer Biology 2022Quote: ... are proportional to the amount of tmCLIC1 and were normalized to values of samples incubated only with secondary antibody (1:400, Donkey anti-mouse conjugated to Alexa Fluor 555, Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed twice and incubated with an anti-mouse secondary antibody conjugated to Alexa Fluor 488 (Thermo Fisher Scientific, # A11001) at a 1:1000 dilution in PBS-T ...
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bioRxiv - Zoology 2023Quote: ... stained with 4′,6-diamidino-2-phenylindole (DAPI) (1:2000 dilution) and Alexa Fluor-conjugated donkey-anti-mouse (Molecular Probes, 1:1000), in PBST at room temperature for 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated at room temperature for 1 hour with goat anti-mouse IgG conjugated to Alexa Fluor 568 (1:250, Invitrogen, cat # A11011). The antibody solution was removed ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were washed in PBS and were incubated with 4ug/ml of secondary goat anti-mouse IgG (H+L) antibody conjugated with Alexa Fluor 633 (Invitrogen, A-21052) and 2ug/ml of secondary goat anti-rabbit IgG (H+L ...
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bioRxiv - Bioengineering 2023Quote: ... sections were washed in 1x PBS three times for 10 min each and incubated with goat anti-mouse IgG Alexa Fluor 488 (Invitrogen; 1:500) and anti-human IgG Alexa Fluor 647 (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... then washed three times for 10 min each in TBST and incubated with secondary antibodies (anti-mouse Alexa Fluor 488, cat. no. A11001; Invitrogen; 1:250)/0.1% Tween20/3% BSA/PBS for 1 h at room temperature in the dark ...