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Citations for Thermo Fisher :
9651 - 9700 of 10000+ citations for Hydrogen Peroxide Fluorescent Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... The thermal cycling and signal detection were performed in duplicate by use of StepOne Real-Time PCR System (Applied Biosystems). The relative abundance of all transcripts amplified was normalized to the means of constitutive expression level of ACTIN8 and UBIQUITIN11 according to Pfaffl (2001).
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bioRxiv - Immunology 2023Quote: ... the signal was evaluated after 48 hours by detection of GFP expression in the HEK-293T-hACE2 cells using Attune NxT Acoustic Focusing Cytometer (ThermoFisher) or BD Symphony Flow Cytometry.
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bioRxiv - Zoology 2023Quote: ... Quantitative real-time PCR was performed on a CFX Connect Real-Time PCR Detection System (Thermo Fisher Scientific., MA, USA) using an SYBR Green PCR kit (TransGen Biotech ...
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bioRxiv - Immunology 2023Quote: ... gene-specific PCR products were quantified by an Applied Biosystems 7500 Sequence Detection System (Applied Biosystems, Foster City, CA, USA). The list of primers and their sequences used are summarized in Table S2 ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed in the ABI sequence Detector System (ABI Prism 7000 Sequence Detection System and software: Applied Biosystems) using a final volume of 25 μl containing 2 μl of cDNA from reverse-transcription reaction ...
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bioRxiv - Cell Biology 2022Quote: ... apoptosis was assessed by the detection of fragmented DNA using the Click-iT-Plus TUNEL Assay (Invitrogen, Waltham, Massachusetts, USA) according to the manufactures protocol ...
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bioRxiv - Biophysics 2023Quote: ... cells were incubated with a dye master mix containing CellEventTM Caspase 3/7 Green Detection Reagent (ThermoFisher Scientific, 1:1000) and Zombie NIR fixable viability stain (BioLegend ...
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bioRxiv - Genomics 2022Quote: ... The extracted DNA was not quantified as DNA amounts were below the detection limit of Qubit 2.0 High Sensitivity reagents (Q32581, Thermo Fisher). All extractions were prepared in a PCR workstation with designated equipment for the workstation ...
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bioRxiv - Genetics 2023Quote: ... Quantitative real time PCR (n = 7–8 per genotype) was performed on an ABI 7300 Sequence Detection system (Applied Biosystems) using the PowerUp SYBR Green Master Mix Kit (Applied Biosystems).
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bioRxiv - Biochemistry 2023Quote: ... for chemiluminescence detection after incubating with an appropriate ECL substrate (either Pierce ECL or SuperSignal West Pico Plus, Thermo Scientific).
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bioRxiv - Immunology 2024Quote: ... The detection of protein bands was then carried out using enhanced chemiluminescence (ECL) (SuperSignal West Pico Chemiluminescent Substrate, ThermoFisher, 34577). The chemiluminescent signal was acquired by placing the membrane in a film cassette and exposing it to X-ray film (Kodak ...
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bioRxiv - Plant Biology 2023Quote: ... Transfer to a nylon membrane and detection of biotin-labeled DNA were performed according to the manufacturer’s instructions (Thermo Scientific 20148 LightShift Chemiluminescent EMSA Kit).
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bioRxiv - Bioengineering 2023Quote: ... MSC-EV preparation vesicular structure and canonical surface marker expression were determined using EV bead-based flow cytometry with human CD81 detection beads (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The hybridization signals in the blotted membranes were detected with the substrates of enhanced chemiluminescence detection reagents (Thermo Fisher Scientific), visualized using an ImageQuant LAS 4000 mini-biomolecular imager (GE Healthcare) ...
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bioRxiv - Cell Biology 2024Quote: ... Positive immunostaining bands on the membranes were visualized using ECL Select Western Blotting Detection Reagent (Cytiva) and scanned by iBright 1500 imaging system (Invitrogen).
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bioRxiv - Cancer Biology 2024Quote: ... in a final volume of 10□µl using an ABI Prism 7000 Sequence Detection System (Thermo Scientific, Rockford, IL, USA). The PCR reactions were initiated with a 10-min incubation at 95□°C followed by 40 cycles of 95□°C for 15□s and 60□°C for 60□s ...
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bioRxiv - Neuroscience 2024Quote: ... Immunoreactive bands in cell extracts were visualized using ECL Western Blotting Detection Reagent (Amersham) on iBright CL1500 Imaging System (Invitrogen). For murine synaptosomal extracts ...
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bioRxiv - Biochemistry 2024Quote: ... Relative expression levels were determined by real-time PCR (qPCR) in a 7900 Sequence Detection System (Applied Biosystems, Carlsbad, CA) using the primer sets listed below/in Table 1.
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bioRxiv - Molecular Biology 2024Quote: ... Hybridization experiments were performed using the ULTRAhyb™ Ultrasensitive Hybridization Buffer (InvitrogenTM) and Chemiluminescent Nucleic Acid Detection Module (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The membranes were washed extensively with TBST before chemiluminescent detection using SuperSignal West Pico substrate or femto substate (Thermo Scientific) and a ChemiDoc imaging system (Bio-Rad).
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were then washed with PBT for 2 h and stored in 2 % DABCO (Acros Organics, AC112471000).
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bioRxiv - Neuroscience 2021Quote: The cultured cells were incubated with Fura-2-acetoxymethyl ester (Fura-2-AM; Molecular Probes; 2.5 µM) in MEM (0.7 mL ...
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted from the SARS-CoV-2 infected Caco-2 cells using Trizol (Thermo Fisher). The extracted RNA was then reverse transcribed with the reverse primers specific to Nsp12 ...
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bioRxiv - Microbiology 2021Quote: ... LNPs were stained with 2% uranyl acetate for 2 mins and imaged by Talos F200X (Thermo Fisher) at an accelerating voltage of 200 kV ...
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bioRxiv - Bioengineering 2022Quote: RPTEC organoids were imaged every 2-3 days with a EVOS FL 2 Auto microscope (Thermo Fisher) with a 4x objective ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL malt extract (2% w/w) in 4 mL screw neck vials (Fisher Scientific, Loughborough, UK) were inoculated with an agar piece of the G ...
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bioRxiv - Developmental Biology 2022Quote: ... 2 µl cDNA was combined with 25 µl 2× TaqMan Gene Expression Master Mix (Thermo Fisher Scientific), 2.5 µl of 20× TaqMan Gene Expression Assay (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... Lipofections were performed using 2 μg gRNA plasmid and 2 μg donor construct in OptiMEM medium (GIBCO) and Lipofectamin Stem reagent (Invitrogen ...
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bioRxiv - Genomics 2019Quote: ... Purified PCR products were sequenced and analyzed with the Sequence Scanner Software 2 Version 2 (Applied Biosystems).
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bioRxiv - Immunology 2020Quote: ACE-2 protein (hsACE-2, residues S19 to D615) was transiently transfected into HEK293E cells (Thermo Fisher) with PEI MAX (Polysciences ...
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bioRxiv - Biochemistry 2022Quote: ... The peptide sample was loaded onto the precolumn (75 μm×2 cm, C18, 2 μm, Thermo Fisher) at a flow rate of 400 nL/min and then separated using a reversed-phase analysis column (50 μm×15 cm ...
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bioRxiv - Cell Biology 2022Quote: ... in HBSS (Hanks Balance Salt Solution) without Ca+2 and Mg+2 (Thermo Fisher Scientific, Waltham, MA.) for 30 min ...
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bioRxiv - Immunology 2022Quote: ... Transferences were done in an iBlot 2 gel transfer device using iBlot 2 transfer nitrocellulose stacks (Invitrogen). Membranes were blotted against phospho-AKT (Ser473 ...
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bioRxiv - Cancer Biology 2024Quote: ... packaging plasmid psPAX2 and pMD2G in a ratio of 2:2:1 using lipofectamine 3000 (ThermoFisher, L3000001) and transduced into NES cells and ONS76 cells ...
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bioRxiv - Genomics 2024Quote: ... each with 2 µg PCR input and 2 µL FastDigest® LguI (Thermo Fisher Scientific, Waltham, MA). These reactions were then incubated at 37°C for two hours ...
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bioRxiv - Cell Biology 2024Quote: ... adipose tissue (2∼4 cm3) was washed twice with PBS solution (AQ) containing 2% penicillin-streptomycin (Invitrogen), and then cut to 1∼2 mm3 with scissors ...
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bioRxiv - Cell Biology 2023Quote: ... 2×106 of the remaining cells were stimulated with 2×106 anti-CD3/CD28 beads (Gibco, 11453D) in 5 ml of IMDM medium supplemented with 10 % FBS ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclear stain: 4′,6′-diamidino-2-phenylindole dihydrochloride (DAPI, blue; 2 ng ml−1; Molecular Probes, USA). Sections were cover-slipped using ProLong Gold anti-fade reagent (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... 2′–5′ OA and dephosphorylated OH-2′–5′ OA were transfected into cells using Lipofectamine 2000 (Invitrogen) according to the protocol provided by the manufacturer.
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bioRxiv - Biophysics 2023Quote: ... Cell nuclei were stained with 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 2 ng/ml; Molecular Probes). Stained sections were imaged using epifluorescence and deconvolution epifluorescence microscopy on an Olympus IX83 ...
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bioRxiv - Cancer Biology 2024Quote: ... OPM-2 cells were labelled with 2 μg/mL of Vybrant DiD cell-labeling solution (Life Technologies). The labeled cells were suspended in RPMI containing 2 mM EDTA ...
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bioRxiv - Cell Biology 2022Quote: ... Assay plates were created by spotting 4 μL/well (1.5 pmol/well) from the intermediate plate onto 384-Well Optical Bottom Plates (Nunc, Cat#152041, same lot in the screening) with the Biomek FXP ...
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bioRxiv - Biophysics 2023Quote: 50 µl of AP205 VLP solution (5 µg/ml) was pipetted into an appropriate number of wells of ELISA plates (4 Nunc MaxiSorp flat bottom 96-well plates) and the VLP allowed to adsorb overnight at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... then individual seeds were grown in separate wells of a 96-micro well plate (Nunc 96F microwell white polystyrene plate, Thermo Fisher Scientific, Waltham, MA, United States) containing 150 μl of Murashige and Skoog (MS ...
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bioRxiv - Biochemistry 2023Quote: ... 10µM of each design were diluted in PBS in triplicates and placed in a 96-well plate (20 MicroAmp Fast Optical 96W Reaction Plate, Thermo Fisher, and MicroAmp Optical Adhesive Film). A ViiA7 real-time PCR instrument (Applied Biosystems ...
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bioRxiv - Biophysics 2021Quote: ... Plates coated with 150 µl of 1% ultra-pure agarose (Invitrogen) in each well were cooled for 30 min prior to adding 1 ml of cell suspension containing 2000-2500 cells ...
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bioRxiv - Biophysics 2020Quote: ... The plate was covered with clear polyolefin sealing tape (Thermo Scientific) to prevent evaporation and placed in a Biotek Synergy HTX multi-mode plate reader ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary cultures were grown on collagen-coated plates in MCDB105 (Gibco) plus supplements ...
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bioRxiv - Cancer Biology 2021Quote: A mouse-specific ProcartalPlex™ Multiplex Immunoassay (26) plate from Invitrogen Thermo Fisher Scientific was used to detect serum cytokine levels ...
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bioRxiv - Molecular Biology 2020Quote: ... the cells were incubated in 384 white plates (Thermo Fisher Scientific), and the measurement of ROS was performed according to the manufacturer’s protocol (Promega).