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Citations for Thermo Fisher :
9601 - 9650 of 10000+ citations for Hydrogen Peroxide Fluorescent Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Quantitative real-time PCR (qPCR) was performed using a Step One Plus detection system (Applied Biosystems, Life technologies, the Netherlands) using Taqman assays (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... The arrays were washed again with distilled water (G biosciences) and incubated with a detection buffer with Cy5-streptavidin (ThermoFisher) at 1:1000 for 20 minutes at RT protected from light ...
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bioRxiv - Immunology 2021Quote: ... Murine IL-2 ELISA capture (catalog no. 14-7022-85) and detection (catalog no. 13-7021-85) antibody reagents were purchased from Invitrogen/eBioscience ...
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bioRxiv - Molecular Biology 2020Quote: All quantitative PCR (qPCR) experiments were conducted using an ABI 7900HT sequence detection system (Perkin-Elmer Applied Biosystems, Warrington, UK). Reactions were performed in 6 μL volumes on 384-well plates in reaction buffer containing 3 μL of 2 x Kapa Probe Fast qPCR Master Mix (Sigma-Aldrich ...
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Activation of endoplasmic reticulum stress via clustering of the inner nuclear membrane protein SUN2bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies used for immunofluorescence detection in confocal and SIM microscopy were Alexa Fluor Rabbit-anti-Mouse 568 (Invitrogen, A11004) and Donkey-anti-Mouse 647 (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and incubated overnight in a shaker protected from light at 4°C with respective detection reagents (DyLight 488-conjugated streptavidin at 1:400 dilution, Invitrogen #21832 ...
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Ferroportin Depletes Iron Needed for Cell Cycle Progression in Head and Neck Squamous Cell CarcinomabioRxiv - Cancer Biology 2022Quote: ... Real Time qRT-PCR was performed using SYBR-green based detection on a Quant Studio 3 real time PCR system using PowerUP Syber green MasterMix (ThermoFisher). Primers for GAPDH and beta-actin were used as endogenous controls to which RNA levels of target genes were normalized to ...
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bioRxiv - Evolutionary Biology 2022Quote: HPLC with mass spectrometry detection was done on a quadrupole LC-MS (Ultimate 3000 UPLC/ISQ EC, Thermo Fisher Scientific) in Single Ion Monitoring (SIM ...
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bioRxiv - Cancer Biology 2022Quote: ... Secondary antibody detection was performed with Alexa Flour 488 goat anti-mouse and Alexa Fluor 594 goat anti-rabbit (Invitrogen). Cells were counterstained with DAPI (1:10,000 ...
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bioRxiv - Genetics 2019Quote: ... and mouse-anti-Prospero (DSHB) followed by secondary detection with anti-rabbit or anti-chicken Alexa 488 and anti-mouse Alexa 555 (Invitrogen).
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bioRxiv - Pathology 2019Quote: ... Sections for the detection of autoantibodies accumulation were blocked and permeabilized with 0.5% Triton X-100 (85111, Thermo Fisher Scientific) and blocked with 2.5% bovine serum albumin solution (BSA ...
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bioRxiv - Biochemistry 2019Quote: ... Real-time PCR was performed on a Step-One Plus Real-Time PCR Detection System (Applied Biosystems, Waltham, MA, USA) using 2X Power SYBR Green Mastermix (ABI ...
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bioRxiv - Microbiology 2019Quote: ... Primary antibodies were detected with peroxidase conjugated goat secondary antibody and enhanced chemiluminescence (ECL) western blotting detection reagent (Thermo Scientific).
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bioRxiv - Genomics 2020Quote: ... The amplification and detection of specific DNA was achieved using the AB 7500 FAST Real-Time PCR System (Applied Biosystems) and the following programme ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Proteins were visualized by chemiluminescent detection (Image Quant) of peroxidase substrate activity (SuperSignal West Femto Maximum Sensitivity Substrate, Thermo Scientific).
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bioRxiv - Microbiology 2019Quote: ... Detection was accomplished with a secondary stain of Alexa Fluor 488 and Alexa Fluor 594 anti-mouse IgG antibodies (Invitrogen). Nuclei were counterstained with 4,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cell Biology 2019Quote: ... Reactions were run in duplicate for each sample and quantified using the ABI Prism 7900 sequence detection system (Applied Biosystems). Duplicates were checked for reproducibility ...
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bioRxiv - Biochemistry 2020Quote: ... The quantitative RT-PCR reactions were performed on an Applied Biosystems 7500 Sequence Detection system (Applied Biosystems, Foster, CA, USA) using ChamQ SYBR Color qPCR Master Mix (Nanjing Vazyme BioTech Co.,Ltd.) ...
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bioRxiv - Molecular Biology 2021Quote: ... Visual detection was accomplished by imaging the tubes through E-Gel™ Safe Imager™ Real-Time Transilluminator (Thermo Fisher).
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bioRxiv - Physiology 2021Quote: ... We performed the assay using a modified 1-plex protocol for Fast Blue Detection using Type 1 Mouse AGER probe and Type 1 Mouse GAPDH probe (Affymetrix). The sections were counterstained with Nuclear Fast Red ...
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bioRxiv - Synthetic Biology 2021Quote: ... Real‐time PCR amplification was performed on 12.5 ng cDNA with the ABI Prism 7300 sequence detection system (Applied Biosystems), using the PowerUp™ SYBR® Green Master Mix (Applied Biosystems) ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were washed a further 3 times for 10 min in TBS with 0.2% Tween 20 and prepared for chemiluminescent signal detection using SuperSignal West Pico chemiluminescent substrate (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... ND4 and APP was performed simultaneously within the same well using a 7500 fast sequence detection system (Thermo Fisher Scientific). Efficiency of the triplex reaction was measured prior sample analysis ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were transferred onto PVDF membrane (Cytiva) and detected using either enhanced chemiluminescence detection (SuperSignal West Pico Chemiluminescent Substrate; ThermoFisher) or fluorescent secondary antibody (ThermoFisher) ...
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bioRxiv - Pathology 2021Quote: ... 35 mins in MES buffer for aSyn detection) and transferred to nitrocellulose membranes (0.2 μm) via Iblot (7 mins, 20 mV; Fisher Scientific). Initial blots were tested for optimisations of aSyn retention as labelled by MJFR1 either by pre-blocking heat treatment (microwaved ...
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bioRxiv - Genomics 2020Quote: ... before membrane washing and incubation with appropriate HRP-conjugated secondary antibodies (Amersham) and detection with SuperSignal ECL West Pico PLUS chemiluminescence (ThermoFisher).
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bioRxiv - Microbiology 2020Quote: ... Quantification of these genes was performed using SYBR Green on a ViiA™ 7 sequence detection system (Applied Biosystems, USA) with two replications for each sample ...
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bioRxiv - Immunology 2020Quote: ... Quantitative real-time polymerase chain reaction was performed using the ABI Prism 7000 Sequence Detection System (Life Technologies, Carlsbad, CA). Run method ...
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bioRxiv - Developmental Biology 2022Quote: ... Secondary antibodies were utilized at a concentration of 1:20,000 and chemiluminescence was performed with ECL Primer Western Blotting detection reagent (Fisher Scientific). Imaging of membranes was performed using an iBright chemiblot system.
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bioRxiv - Cell Biology 2022Quote: ... The quantification of PCR products was analyzed with SYBR green using ABI PRISM 7900 Sequence Detection system software (Applied Biosystems). The primer sequences were MajSAT 5’-GGCGAGAAAACTGAAAATCACG-3’,5’-CTTGCCATATTCCACGTCCT-3’ ...
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bioRxiv - Molecular Biology 2022Quote: ... Detection probes for phage DNA in Southern blots were generated by PCR using a non-proofreading polymerase (DreamTaq polymerase, ThermoFisher) using oligonucleotides specified in Table S4 ...
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bioRxiv - Cancer Biology 2022Quote: ... EdU detection was performed per the manufacturer’s instructions with 500 μL of Click-iT® reaction cocktail ((Invitrogen, Cat# C10337)) per coverslip ...
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bioRxiv - Genetics 2022Quote: ... and analyzed using Sequence Detection System Software v.2.1 or QuantStudio Design and Analysis Software v.1.2 (Applied Biosystems, MA). Expression was measured in technical triplicates and the averages of the threshold cycle (Ct ...
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bioRxiv - Genetics 2022Quote: ... secondary antibodies and visualized using ECL Prime Reagent for chemiluminescent detection on Hyperfilm ECL (Amersham) or iBright FL1500 Imaging system (ThermoFisher). Western band intensities were quantified using Fiji.
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bioRxiv - Plant Biology 2022Quote: ... The detection of the isotopologues was made by using the software Xcalibur® 2.1 (Thermo Fisher Scientific, Waltham, MA, USA). The fractional 13C-enrichment was then performed as described earlier (Lima et al. ...
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bioRxiv - Microbiology 2022Quote: ... and stained with 1 μg/ml of mAb 2A10 in 1% BSA in PBS (pH 7.4) followed by detection with Alexa fluor 594 goat anti-mouse secondary antibody (Invitrogen, A11032). Samples were mounted in gold antifade mountant (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... CellEvent™ Caspase-3/7 Green Detection Reagent (C10723) and PrestoBlue™ Cell Viability Reagent (A13261) were purchased from ThermoFisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... PCR reactions were performed on 10 ng of cDNA in the ABI PRISM 7900HT Sequence Detection System (Thermo Fisher Scientific) using isoform specific primer pairs giving 90100% efficacy and a single amplicon at the appropriate melting temperature and size (Table Supplement 2) ...
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bioRxiv - Neuroscience 2022Quote: ... WAKO) overnight at 4 °C followed by detection with Alexa 488 conjugated anti-rabbit secondary antibody (1:1000, Life Technologies), and Alexa 594 conjugated anti-rat secondary antibody (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... Detection of the eYFP-tag was performed by using primary rabbit antibodies against GFP (1:5000) (Invitrogen A6455, lot #1853896) and secondary anti-rabbit antibodies conjugated with horseradish peroxidase (1:10,000 ...
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bioRxiv - Neuroscience 2024Quote: ... The secondary antibodies that were used for detection were the following: Alexa Fluor™ 488 donkey-antirrabit (Invitrogen, 1:500) against glial fibrillary acidic protein (GFAP ...
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bioRxiv - Immunology 2024Quote: ... The detection of protein bands was then carried out using enhanced chemiluminescence (ECL) (SuperSignal West Pico Chemiluminescent Substrate, ThermoFisher, 34577). The chemiluminescent signal was acquired by placing the membrane in a film cassette and exposing it to X-ray film (Kodak ...
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bioRxiv - Plant Biology 2023Quote: ... Transfer to a nylon membrane and detection of biotin-labeled DNA were performed according to the manufacturer’s instructions (Thermo Scientific 20148 LightShift Chemiluminescent EMSA Kit).
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bioRxiv - Microbiology 2023Quote: ... The hybridization signals in the blotted membranes were detected with the substrates of enhanced chemiluminescence detection reagents (Thermo Fisher Scientific), visualized using an ImageQuant LAS 4000 mini-biomolecular imager (GE Healthcare) ...
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bioRxiv - Cell Biology 2024Quote: ... and protein detection was carried out using enhanced chemiluminescence (ECL) reagent (SuperSignal West Femto Maximum Sensitivity Substrate, Thermo Fisher Scientific). Primary antibodies used were ...
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bioRxiv - Molecular Biology 2024Quote: ... was performed on a Bio-Rad CFX384 Real-Time PCR Detection System using PowerUPTM SYBRTM Green Master Mix from ThermoFisher Scientific (catalog #A25742) ...
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bioRxiv - Cancer Biology 2024Quote: ... the media was replaced with DMEM + 10% FBS + CellEvent Caspase-3/7 Detection Reagent (Green) according to the manufacturers recommendations (Invitrogen). The plate was then imaged every 15 minutes using phase and GFP channels in the ZEISS Celldiscoverer 7 Automated Live Cell Imager set to 42°C at 5% CO2 and 20% O2 ...
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bioRxiv - Cell Biology 2024Quote: ... The following secondary antibodies were used for detection: goat anti-rabbit DyLight 800 (1:10000; Thermo Fisher Scientific; SA5- 35571), goat anti-mouse DyLight 800 (1:10000 ...
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bioRxiv - Cell Biology 2024Quote: The specific detection and measurement of mitochondria ROS in embryonic epithelium was conducted by using MitoSOX Red mitochondrial superoxide indicator (Invitrogen). Embryonic lungs at E13.5 were harvested and disassociated in the lysis buffer (PRMI 1640 (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time PCR was performed using the StepOnePlus Real-Time PCR detection system (Applied Biosystems, Foster City, CA, USA) and Platinum SYBR Green qPCR super Mix (Invitrogen) ...