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Citations for Thermo Fisher :
9551 - 9600 of 10000+ citations for Hydrogen Peroxide Fluorescent Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Sections in the vicinity of electrodes were mounted onto 0.1% gelatin-subbed slides and stained with NeuroTrace 530/615 Red Fluorescent Nissl Stain (N21482, ThermoFisher Scientific, Waltham, MA, USA) to confirm proper placement (Fig ...
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bioRxiv - Neuroscience 2024Quote: ... Sections contaiing the electrode sites were then mounted on histology slides and labeled with a green fluorescent Nissl stain (Invitrogen NeuroTrace, catalog# N21480). Slides were then imaged at 2x using an automated fluorescent microscope (Keyence BZ-X800) ...
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bioRxiv - Neuroscience 2024Quote: ... nuclei and LDs were counterstained with DAPI and the following fluorescent dyes: BODIPY 493/503 4,4-DIFLUORO-1,3,5,7,8-PE (Fisher Scientific 11540326, dilution 1:1000 in 1X PBS); LipidSpot488 (VWR 70065-T ...
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bioRxiv - Neuroscience 2024Quote: ... The electrode array was stained with a fluorescent dye (cell tracker CM-Dil dye, red excitation/emission spectra: 553/570 nm; ThermoFisher Scientific, MA, USA) just before it was inserted into the brain ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were spun down at 1000 rpm for 4 min and resuspended in PBS plus LIVE/DEAD™ Fixable Dead Cell Stain Near-IR fluorescent reactive dye (Thermo Fisher; L34976) and incubated for 30 min protected from light ...
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bioRxiv - Synthetic Biology 2024Quote: ... Green fluorescent protein (GFP) standard curve was created from dilutions of Aequorea Victoria GFP His-tag recombinant protein (ThermoFisher Scientific, Cat. No. A42613) and measured on the BioTek Cytation5 Imaging Reader ...
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bioRxiv - Genetics 2021Quote: ... cDNA was synthesized by incubating RNA for 2 h at 37 C using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). Afterwards ...
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bioRxiv - Microbiology 2019Quote: ... and monoclonal mouse IgG α-PAN-pLDH (PA-2, AccessBio, Somerset, NJ) were biotinylated using the EZ-Link Sulfo-NHS-Biotin Kit (21435, Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions with minor modifications (See Additional file 1 ...
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bioRxiv - Cancer Biology 2019Quote: ... and 1-2 μg of total RNA was used for synthesizing cDNA with High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, Waltham, MA, USA). cDNA was then used for Q-PCR in a StepOnePlusTM (Applied Biosystems ...
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bioRxiv - Synthetic Biology 2021Quote: ... the amplicons were gel-verified (2-µl aliquots were run on a 1% agarose gel) and column-purified (GeneJET PCR purification Kit, Thermo Fisher Scientific, catalog no. K0701). For cloning into ArEc-TDH3 ...
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bioRxiv - Plant Biology 2020Quote: cDNA was synthesized from 2 μg of RNA using random primers in 20 μL reaction with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA).
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bioRxiv - Plant Biology 2021Quote: ... Total RNA was treated with 2 U of DNase per 10 μg of total RNA using the TURBO DNA-free™ Kit (Thermo Fisher Scientific, former Ambion). Integrity of DNA-free RNA was verified in Bleach Gel (Aranda et al. ...
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bioRxiv - Microbiology 2020Quote: ... all end-point rpoB and 16S gene amplicons were pooled at equimolar concentrations (∼1-2 ng/µl) using a SequalPrep normalization kit (Thermo Fisher Scientific, Waltham, MA, USA) and purified by gel extraction using the Wizard SV gel and PCR clean-up system (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... First-strand cDNA was synthesized from 2 µg of RNAusing High-Capacity cDNA Reverse Transcription Kits (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, United States). RT-qPCR was done using PowerUp™SYBR™Green Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... RNA concentrations in the purified samples were determined using a Qubit RNA HS kit with a Qubit 2 Fluorometer (Thermo Fisher Scientific, Scoresby, VIC, Australia). First-strand DNA synthesis was performed using 200ng purified RNA and SuperScript IV VILO Master Mix with ezDNase Enzyme (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2019Quote: ... they were further incubated with a secondary HRP-conjugated goat anti-rabbit IgG antibody for 2 hours at room temperature and imaged using a supersignal west pico enhanced chemiluminescence kit (Thermo Fisher Scientific, Suwanee, GA,USA). The signals were visualized with UVP software.
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bioRxiv - Plant Biology 2020Quote: Young seedlings were incubated in 20 μM 5-ethynyl-2’-deoxyuridine (EdU) (Click-iT™ EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit, ThermoFisher Scientific/Invitrogen, Waltham, Massachusetts, USA) made in ddH2O for 30 min at 24 °C ...
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bioRxiv - Immunology 2021Quote: ... In order to assess proliferation T cells were resuspended at 1 × 106 cells/ml using a 2 μM solution of CellTrace™ Violet Cell Proliferation Kit (Thermo Fisher Scientific, Massachusetts, USA) in PBS ...
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bioRxiv - Microbiology 2024Quote: ... DNA Fragments were subsequently cloned into a PCR-linearized pZE21 expression vector (primers 1 and 2) through blunt-ended ligation (Thermo Fisher Rapid DNA Ligation kit). Ligated products were run on a 0.5% agarose gel to isolate fragments between 5 and 10 kb ...
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bioRxiv - Evolutionary Biology 2023Quote: ... with each sample being prepared from 5 individuals (3 karyotypes x 2 temperatures x 9 isochromosomal lines = 54 samples) using the MagMAX™-96 Total RNA Isolation Kit (ThermoFisher Scientific, Waltham, MA, USA) on a MagMAX™ Express Magnetic Particle Processor (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... The secondary antibodies used for detection were horseradish peroxidase (HRP)–conjugated goat anti-mouse and goat anti-rabbit IgG (1:5,000; Thermo Scientific). The blots were incubated with Luminata Western HRP substrate (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... was used as secondary antibody at 1:10,000 dilution and the detection was achieved with the ECL Femto reagent (Thermo Fisher). The V5 tag was detected with an anti-V5 antibody coupled with HRP (SantaCruz ...
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bioRxiv - Cell Biology 2020Quote: ... primary antibody at a dilution of 1:100 and secondary detection was performed using donkey anti-rabbit Alexa 488 antibodies (Invitrogen) at a dilution of 1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... primary antibody at a dilution of 1:100 and secondary detection was performed using donkey anti-mouse Alexa 647 antibodies (Invitrogen). pRB staining was performed following EdU staining by first permeabilizing the cells and then blocking in donkey serum for 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... qPCR was performed using SYBR green Supermix (OZYME) in an ABI7500 Prism SDS Real-Time PCR Detection System (Applied Biosystems). The mRNA content was normalized to β-actin mRNA and quantified using the 2-ΔΔCt method ...
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bioRxiv - Neuroscience 2021Quote: ... and GFAP using the Applied Biosystems™ ABI PRISM™ 7000 Sequence Detection System (Applied Biosystems, ThermoFisher Scientific, Waltham, MA) and Rotor-Gene Q (Qiagen ...
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Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: ... Protein detection was done using a custom antibody against MIPS (1:10,000) and anti-actin (1:10,000; Invitrogen MA1-744). The custom MIPS antibodies were produced by YenZym Antibodies ...
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bioRxiv - Genomics 2020Quote: ... The qPCRs were run of both technical and biological replicates (n=3) using an ABI7500 sequence detection system (Applied Biosystems by Life Technologies ...
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bioRxiv - Cancer Biology 2019Quote: All of the PCR reactions were performed using an ABI Prism 7900HT Sequence Detection system (Thermo Fisher Scientific, Waltham, Massachusetts). PCR was performed using the Power SYBR™ Green PCR Master Mix (Life Technologies ...
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bioRxiv - Immunology 2019Quote: ... Cycling was performed in duplicate using a Step One Plus™ Real Time detection system (Applied Biosystems, Foster City, CA). Gene-specific amplification was normalized to GAPDH as an internal reference gene and gene expression was normalized relative to uninfected MDSC controls using the formula 2-ΔΔCt.
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bioRxiv - Neuroscience 2021Quote: ... Quantitative RT-PCR reaction in real time was done on an ABI Prism ® 7500 Sequence Detection System (Applied Biosystems), using System SYBR-green fluorescence (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... and GFAP using the Applied Biosystems™ ABI PRISM™ 7000 Sequence Detection System (Applied Biosystems, ThermoFisher Scientific, Waltham, MA) and Rotor-Gene Q (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: Feature detection and peak alignment from .Raw files were performed using Compound Discoverer 3.2 (Thermo Fisher Scientific, Waltham, MA, USA). Files were also converted to .mgf format using MSConvert software (ProteoWizard ...
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bioRxiv - Neuroscience 2020Quote: Glutamate concentration in microdialysis samples was quantified using isocratic high-performance liquid chromatography (HPLC) with electrochemical detection (Thermo Fisher Scientific). O-pthalaldehyde (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... Both membranes were subsequently incubated for 1 hour at RT with the Clean-Blot IP Detection Reagent (HRP, Thermo Scientific). After washing the membrane three times with 1x TBST ...
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bioRxiv - Neuroscience 2021Quote: ... was used for the PCR reaction was used according to the manufacturer’s protocol in a QuantStudio3 Detection System (Applied Biosystems). Each sample was run in duplicate and glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... followed by internalization into target cells and detection by incubation with secondary anti-human-Alexa 555 fluorophore conjugates (Thermo Scientific). Cells were examined by immunofluorescence analysis performed on an Axio Observer Z1 widefield epifluorescence microscope (Zeiss Inc. ...
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bioRxiv - Cell Biology 2020Quote: To detect proliferation/cell cycle entry we utilized the EdU/click-it detection platform to visualize cells that contained newly synthesized DNA (Cat nb. C10337 or C10340, Invitrogen, Thermofisher). For experiments performed immediately post FACS ...
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bioRxiv - Cell Biology 2021Quote: ... MS-HRM-PCR analysis was done in 7500 FAST Real Time ABI Prism Sequence Detection System (Applied Biosystems, Boston, EUA) using temperature ramp and acquisition of fluorescence in accordance with the manufacturers’ recommendation ...
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bioRxiv - Cancer Biology 2020Quote: ... Gene expression was assessed from 10 ng cDNA at the ABI 7900 HT system using SYBR green detection and the SDS (v2.4) software (Applied Biosystems). For normalization the two housekeeping genes HPRT1 and GNB2L1 were used ...
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bioRxiv - Bioengineering 2022Quote: ... conjugated to horseradish peroxidase (HRP) were used for detection of proteins using ECL Western Blot Substrate Reagent (Thermo Fisher Scientific). Images were acquired using a Chemidoc Imaging System and analyzed with Image-Lab software (Bio-Rad).
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bioRxiv - Cell Biology 2022Quote: ... Samples were washed 3 more times in 1x PBS before DNA detection with 1 µg/ml Hoechst 33342 (Invitrogen, H3570) for 15 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time RT-PCR measurements were performed using the Step One Plus Real-Time Detection System (Thermo Scientific, Waltham, USA). Reactions were performed in triplicate in a total volume of 20 μl using the maxima SyBR green qPCR master mix according to manufacturer’s instructions (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: ... or mouse antibody to Myc (1:2000, Sta. Cruz) and Clean-Blot IP detection reagent (HRP) (1:5000, Thermo Scientific).
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bioRxiv - Developmental Biology 2022Quote: ... All QPCR reactions were performed in duplicate on the 7500 ABI Prism Sequence detection System (Applied Biosystems, Foster City, CA). The cycling conditions included a denaturation step of 95°C for 10 mins followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min ...
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bioRxiv - Microbiology 2022Quote: ... then washed three times with PBS and incubated with secondary antibodies (Table 4) and phalloidin (F-actin detection) (1:100) (Invitrogen) for 1 hr at room temperature ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Quantitative real-time PCR (qRT-PCR) was performed in an ABI StepOnePlus instrument with the SYBR green detection system (Invitrogen). PCR thermal profiles were 40 cycles with 10 s at 95 °C and 30 s at 60 °C each cycle ...
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bioRxiv - Cancer Biology 2022Quote: Detection of eluting peptides is performed using both UV at 214 nm (3nl UV cell, Thermo Fisher Scientific, Germering, Germany) and mass spectrometry (Q-Exactive Plus ...
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bioRxiv - Microbiology 2020Quote: ... The thermal cycle and fluorescence detection were performed with the QuantStudio 12K Flex Real-Time PCR System (Thermo Fisher Scientific) following manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... Quantitative real-time PCR (qPCR) was performed using a Step One Plus detection system (Applied Biosystems, Life technologies, the Netherlands) using Taqman assays (Applied Biosystems ...