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Citations for Thermo Fisher :
9501 - 9550 of 10000+ citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The concentration of nucleic acids was assessed by fluorimetric measurement through QuBit 3.0 and the amount of amplifiable DNA (sequencing-grade quality) was determined using a quantitative assay (TaqMan RNAseP detection assay) on a StepOnePlus instrument (both: Thermo Fisher Scientific). Samples were amplified using a custom-designed gene panel for breast cancer32 covering the most recurrent mutations33 ...
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bioRxiv - Bioengineering 2021Quote: ... To compare protein quantity between the medulla and cortex a standard method described in the Taqman Protein Assays Sample Prep and Assay (Thermo Fisher, 4453745) was used ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were centrifuged at 13000 rpm for 15 min and the supernatant was used to perform the protein assay using the bicinchoninic acid assay (Thermo Fisher Scientific). Inflammatory biomarkers (LCN-2 ...
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bioRxiv - Neuroscience 2020Quote: ... TdT-mediated dUTP Nick-End Labeling (TUNEL) assay was performed using Click-iT Plus TUNEL Assay with Alexa Fluor 488 (Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... 10 ng of total RNA was reverse-transcribed and amplified using TaqMan miRNA gene expression assay (TaqMan® MicroRNA assay #002284, Applied Biosystems) following manufacturer’s protocols ...
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bioRxiv - Microbiology 2021Quote: ... Libraries were validated for length and adapter dimer removal using the Agilent TapeStation 4200 D1000 high-sensitivity assay and then quantified and normalized using the double-stranded DNA (dsDNA) high-sensitivity assay for Qubit 3.0 (Life Technologies, Carlsbad, CA). Libraries were run on an Illumina High Output 75-cycle v2.5 NextSeq 500 flow cell ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... was performed using Taqman reagents and specific primer and probe sets for human SLC22A24 (Assay ID: Hs00543210_m) and GAPDH (Assay ID: Hs99999905_m1) (Applied Biosystems, Foster City, CA). Reactions were performed in a 96-well plate with a 10 µL reaction volume using an ABI 7900HT Fast Real-time PCR system (Applied Biosystems ...
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bioRxiv - Biochemistry 2021Quote: ... All the measurements were done in triplicates (from three independent assays) at RT in 96-well microplates for fluorescence-based assays (Invitrogen, REF M33089).
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bioRxiv - Neuroscience 2020Quote: ... ATP quantities were finally referred to protein amount using the Bradford assay (Coomassie Plus™ Protein Assay Reagent, Thermo Scientific, Schwerte, Germany).
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bioRxiv - Genomics 2021Quote: The cell proliferation assay was performed in sterile 96-well round bottom microwell plates using CyQUANT direct cell proliferation assay (Thermo Fisher Scientific). The assay was performed on ~ 5,000 of L1236 or L428 cells in 100 μl of growth medium per well ...
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bioRxiv - Neuroscience 2022Quote: ... Then we evaluated the gene expression levels using TaqMan Gene Expression Assays for Casp3 (TaqMan® Gene Expression Assays cat#00563962; Applied Biosystems), employing 18S ribosomal RNA (TaqMan® Gene Expression Assays cat#4333760 ...
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bioRxiv - Neuroscience 2022Quote: ... 10 ng of total RNA was reverse-transcribed and amplified using TaqMan microRNA gene expression assay (TaqMan® MicroRNA assay cat#002284, Applied Biosystems) following the manufacturer’s protocols ...
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bioRxiv - Immunology 2024Quote: The following reagents were added either during motility assay or in cell culture for the indicated period of time before the assay: 11mM glucose (Thermo Fisher A2494001), 2mM or 11mM glutamine ...
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bioRxiv - Molecular Biology 2023Quote: HEK293 cells transfected with WT or mutant GPR40 plasmids for IP1 assay and or Binding Assay were harvested with TrypLE (Thermo Fisher Scientific), resuspended in optiMEM (Life Technologies ...
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bioRxiv - Cell Biology 2024Quote: Cryopreserved primary human hepatocytes were genotyped for PNPLA3 rs738409(G) using TaqMan® SNP Genotyping Assays (Life Technologies, Assay ID C_7241_10, 4351379) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... using dsDNA and ssRNA high sensitivity assays (ThermoFisher Scientific). Synthesis of the first strand of cDNA was carried out using the SuperScript™ II reverse transcriptase system and random primers (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... as measured by Qubit RNA HS assay (ThermoFisher Scientific), was reverse transcribed using Superscript IV reverse transcriptase (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2021Quote: ... Pool concentration was determined by Qubit assay (Thermo Fisher) and size verified by Tapestation (Agilent) ...
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bioRxiv - Cancer Biology 2021Quote: ... organoid were stained with CyQUANT Direct assay (Thermofisher, #C35011) according to the manufacturer’s instructions to detect the alive cells and Hoechst (#H1399 ...
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bioRxiv - Systems Biology 2020Quote: ... Protein concentrations were analyzed with BCA assay (Thermo Scientific Pierce BCA Protein Assay Kit ...
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bioRxiv - Molecular Biology 2020Quote: ... protein concentration was measured by BCA assay (Thermo Scientific), and protein concentrations were equalized prior to boiling in the recommended sample buffers supplemented with 2.5% beta-mercaptoethanol (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... Purified proteins were quantified by Bradford assay (Thermo Scientific).
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bioRxiv - Cell Biology 2020Quote: ... and then quantified using Pierce BCA assay (Thermo Fisher). Identical amounts of proteins (20-40μg ...
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bioRxiv - Microbiology 2020Quote: ... DNA was then quantified by PicoGreen assay (Life Technologies). Fractions were pooled to nine sets ...
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bioRxiv - Immunology 2021Quote: ... a TaqMan custom gene expression assay (Thermo Fisher Scientific) was designed (Wolfel et al. ...
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bioRxiv - Genetics 2021Quote: ... we used the respective TaqMan gene expression assays (ThermoFisher) containing a FAM-labeled probe ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA was quantified using Quant-IT RNA assay (Invitrogen). Stranded RNA-seq libraries were constructed using the Illumina TruSeq Stranded RNA protocol after treatment with Ribozero ...
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bioRxiv - Evolutionary Biology 2021Quote: ... quantified using the Qubit DNA BR assay (Thermofisher Scientific) and diluted to 0.5 ng/µL for genotyping ...
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bioRxiv - Immunology 2022Quote: ... Protein concentrations were determined using BCA assay (ThermoFisher Scientific). 20 µg of total denatured bacterial protein was loaded into each well in SDS gel ...
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bioRxiv - Molecular Biology 2021Quote: ... and the associated Qubit RNA assay (Q32852, ThermoFisher Scientific). RNA integrity was confirmed using an Agilent 2100 Bioanalyzer and the associated RNA 6000 pico kit (Agilent Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... determined by BCA protein assay (Thermo Scientific, Rockford, IL), were loaded onto SDS-PAGE gel and immunoblotted using antibodies against clathrin heavy chain (1:1,000 ...
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bioRxiv - Immunology 2021Quote: ... Protein was quantified by standard BCA assay (Thermo Scientific) as per manufacturer instructions ...
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bioRxiv - Immunology 2021Quote: ... in the TaqMan Gene Expression Assay system (Life Technologies). We use 10 ng of cDNA ...
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bioRxiv - Immunology 2019Quote: ... Gapdh TaqMan Gene Expression Assay (#Mm99999915_g1; Thermo Fisher Scientific) was applied as an internal amplification control ...
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bioRxiv - Cancer Biology 2019Quote: ... using TaqMan™ gene expression assays (Applied Biosystems, USA) containing primers and probes specific for lncRNA and GAPDH ...
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bioRxiv - Physiology 2019Quote: ... Protein concentration was determined by BCA assay (Thermo Scientific) and absence of endotoxin (<0.25Eu/ml ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentration was measured using Bicinchoninic assay (BCA – Thermofisher). Protein extracts were then loaded on 10% SDS-page acrylamide gels and transferred on PVDF membrane (Millipore #IPVH00010 ...
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bioRxiv - Physiology 2020Quote: ... The following SYBER Green Gene Expression Assays (Applied Biosystems) were used in this study Table 1 Each PCR reaction was performed in triplicate ...
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bioRxiv - Pathology 2020Quote: ... Protein concentration was quantified by BCA assay (Thermo Fisher). Ten post-mortem human globes were collected from five non-diabetic controls lacking ocular disease ...
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bioRxiv - Neuroscience 2019Quote: ... and Qubit dsDNA HS Assay (Life Technologies, Carlsbad, CA). Paired end 50 bp reads were obtained for pooled libraries using Illumina HiSeq 2500 at the JHU GRCF High Throughput Sequencing Center.
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bioRxiv - Genomics 2020Quote: ... as measured with the Qubit High Sensitivity assay (Invitrogen), and used 11.5 μl as input to a 25 μl reaction with 12.5 μl of Nextera TD buffer and 1.0 μl of Nextera Tn5 enzyme ...
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bioRxiv - Genetics 2020Quote: ... Taqman assay probes were all purchased from Applied Biosystems corresponding to each individual gene ...
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bioRxiv - Immunology 2021Quote: ... and CXCL10 via enzyme linked immunosorbent assay (ELISA) (ThermoFisher). ELISA data was acquired on a SpectraMax M5 Multi-Mode Microplate Reader (Molecular Devices ...
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bioRxiv - Systems Biology 2020Quote: ... quantified using the Qubit dsDNA High Sensitivity assay (ThermoFisher) then sequenced on a NextSeq 500 using 150 cycles for read 1 and 8 cycles for each index ...
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bioRxiv - Immunology 2021Quote: ... A Taqman custom gene expression assay (Thermo Fisher Scientific) was designed using the sequences targeting the E gene sgRNA ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was quantified by BCA assay (Thermo Fisher). 5-20 μg of protein from total heart lysates were loaded on polyacrylamide gels and processed as described previously on PVDF membranes[21] ...
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bioRxiv - Immunology 2021Quote: RNA was quantified using Qubit RNA HS assay (ThermoFisher). The quality was measured on the Agilent 2100 Bioanalyzer using the Agilent RNA 600 Pico Kit ...
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bioRxiv - Cancer Biology 2020Quote: ... Identification numbers of commercial assays (Life Technologies, Carlsbad, CA) and sequences of the NTNG1 primers (FW and RV ...
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bioRxiv - Biochemistry 2021Quote: ... Commercially designed primers (Taqman gene expression assay, Thermo Fisher) were purchased for FLI1 (Hs04334143_m1) ...
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bioRxiv - Biochemistry 2020Quote: ... using the TaqMan gene expression assay system (Life Technologies).