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Citations for Thermo Fisher :
9401 - 9450 of 10000+ citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... and quantitated via BCA assay (Thermo Scientific PI23227). 30 µg of each well was used for western blot as above ...
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bioRxiv - Molecular Biology 2024Quote: ... using the RNA Broad Range Assay (ThermoFisher, Q10210).
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bioRxiv - Developmental Biology 2021Quote: ... oocytes or embryos were cultured with O-propargyl-puromycin (OP-puro) (obtained as part of a Click-iT® Plus OPP Protein Synthesis Assay Kit; Molecular Probes/Thermo Fisher Scientific) at a concentration of 20 µM for the indicated time periods ...
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bioRxiv - Microbiology 2020Quote: Extracellular proteins were collected from late-log cells grown in NBAF medium as previously described (14) and quantified using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, MA, USA). The amount of non-reducing loading buffer was used to equal the amount of protein ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The subset of individuals used in the 3RAD library prep (see below) were also quantified using the fluorescence-based Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen - Molecular Probes, Eugene, OR, USA). Extracted DNA were stored at −20°C until use.
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bioRxiv - Genomics 2020Quote: ... RNA quality and concentration was then analysed on a Qubit® 2.0 Fluorometer using Qubit RNA BR assay kit (Invitrogen, Life Technologies, Carlsbad, CA, USA). Between 49 and 107 million sequence reads were generated for each sample on an Illumina HiSeq 4000 platform with a single-end sequencing primer over 150 cycles (Additional file 5 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and the protein concentration was determined using the Pierce™ BCA Protein Assay Kit following the manufacturer’s instructions and a Varioskan Lux plate reader (Thermo Fisher Scientific, Waltham, MA, USA). Samples were separated using the Mini-PROTEAN® TGX™ Precast Gel 4-15% (Bio–Rad Laboratories ...
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bioRxiv - Microbiology 2020Quote: ... Germany) and the precise concentration was determined using the Qubit® dsDNA HS Assay Kit as recommended by the manufacturer (Life Technologies GmbH, Darmstadt, Germany). Illumina shotgun libraries were prepared using the Nextera XT DNA Sample Preparation Kit and subsequently sequenced on a MiSeq system with the reagent kit v3 with 600 cycles (Illumina ...
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bioRxiv - Developmental Biology 2019Quote: BeWO cell culture was harvested 48-hours post transfection media was subjected to lactate dehydrogenase (LDH) detection using the Pierce™ LDH Cytotoxicity Assay Kit (Thermo Fisher, Cat No. 88953), according to manufacturer instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... and cell cycle analysis was performed with the Click-iT® EdU Alexa Fluor® 488 Flow Cytometry Assay Kit (Thermo Fisher, Cat No. C10420), in conjunction with 7-Amino-Actinomycin D (Thermo Fisher ...
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bioRxiv - Molecular Biology 2019Quote: ... Cleaved Tn5 was eluted in ~1 mL fractions and Tn5 elution efficiency was monitored by the Pierce™ Coomassie (Bradford) Protein Assay Kit (Thermo Fisher Scientific cat# 23200). Fractions with the strongest absorbance were pooled and dialyzed versus two changes of 2 L of 2X Tn5 dialysis buffer (100 mM HEPES-KOH at pH 7.2 ...
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bioRxiv - Bioengineering 2021Quote: ... RNA purity and concentration were checked using a NanoPhotometer® spectrophotometer (IMPLEN, CA, USA) and Qubit® RNA Assay Kit in Qubit® 2.0 Flurometer (Life Technologies, CA, USA). RNA integrity was assessed using an RNA Nano 6000 Assay Kit from the Bioanalyzer 2100 system (Agilent Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... centrifuged at 15000 x g for 10 minutes and supernatants used to measure total protein concentration with BCA protein assay kit (Thermo Scientific-Pierce, Waltham, MA, USA). Equal amounts of total protein were loaded onto 10% or 14% SDS-PAGE for Western transfer to PVDF membranes and immunoblotting.
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bioRxiv - Systems Biology 2020Quote: ... The quantitative RT-PCR reactions were done using Applied Biosystems™ TaqMan™ RNA-to-CT™ 1-Step Kit and Custom TaqMan Gene Expression Assays (Thermo Fisher Scientific). The standard curve method was used to determine absolute mRNA concentrations for the samples ...
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bioRxiv - Neuroscience 2020Quote: Total protein levels were quantified in tissue homogenates using the Pierce Microplate BCA Protein Assay Kit in triplicates following the manufacturer’s protocol (Thermo Scientific Pierce Protein Biology, Thermo Fisher Scientific, Waltham, MA, USA). All cytokine levels were normalized by total protein levels and reported as pg cytokine/mg of protein.
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bioRxiv - Genomics 2022Quote: RNA quality and quantity were evaluated with NanoDrop™ 2000 (260/280 nm and 260/230 nm ratios) and Qubit™ 3.0 Fluorometer using Qubit™ RNA HS Assay Kit (Thermo Fisher Scientific Inc., USA). The samples were stored at −80 °C until transported to the Experimental Medicine Research and Application Center of the University of Health Sciences in Istanbul for library preparation and sequencing.
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bioRxiv - Genomics 2022Quote: The concentration of the final libraries was measured using Qubit™ dsDNA HS Assay Kit on a Qubit™ 4.0 Fluorometer (Thermo Fisher Scientific Inc., USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: We diluted the RNA samples to 1 ng/μL for library preparation and confirmed the concentration using a Qubit RNA High Sensitivity Assay Kit (Invitrogen™, Thermo Fisher Scientific, Waltham, MA) and Qubit 4 Fluorometer (Invitrogen™ ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein synthesis rate were determined by O-propargyl-puromycine incorporation with the Click-iT™ Plus OPP Alexa Fluor™ 488 Protein Synthesis Assay Kit from Invitrogen (Waltham, MA, # C10456) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... at final concentration of 10 µM for 4 hours and then harvest to perform Click-iT reaction using Click-iT EdU flow cytometry Alexa Fluor 488 assay kit (Life technologies, Carlsbad, CA, USA, C10420). Fluorescence measurements were taken using LSRfortessa X-20 (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: In situ-hybridization on lungs was performed as previously described (71) using the ViewRNA™ ISH Tissue Assay Kit (Invitrogen by Thermo Fisher Scientific, Darmstadt, Germany) according to the manufacturer’s instructions with minor adjustments ...
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bioRxiv - Cancer Biology 2022Quote: ... 10μM EdU was added to the culture media for 2h prior to fixation and detection using the Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific, C10424), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the bacterial community activity was assessed by measuring the protein concentration using Thermo Scientific™ Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, USA) and the ammonia concentration using Sigma-Aldrich® Ammonia Assay Kit (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... The identity and concentration of the purified protein was examined and quantified using the sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and the Pierce Bradford Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Systems Biology 2022Quote: ... The concentration of the glucose produced from the glycogen was determined using the Amplex Red Glucose/Glucose Oxidase Assay kit (Thermo Fisher Scientific, Waltham, MA, USA), according to the manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: The DNA content of the libraries were quantified using a Qubit™ ssDNA Assay Kit on a Qubit™ 3 Fluorometer (Thermo Fisher Scientific, 2017), then pooled in equal DNA proportions ...
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bioRxiv - Cell Biology 2020Quote: ... or specific Taqman microRNA assay primers with a TaqMan® MicroRNA Reverse Transcription Kit for the assessment of microRNAs (4366596, Applied Biosystems™, Thermo Fisher). Downstream qPCR amplifications of first-strand cDNA were performed using TaqMan® Universal PCR Master Mix ...
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bioRxiv - Immunology 2019Quote: The concentration of sPD-1 and sTim-3 was measured in thawed plasma samples by enzyme linked immunosorbent assay (ELISA) using Human PD-1 (PDCD1) ELISA kit [EHPDCD1] (Thermo Fisher Scientific, Waltham, MA USA) and Quantikine ELISA Human TIM-3 Immunoassay kit [DTIM30] (R&D Systems ...
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bioRxiv - Pathology 2020Quote: ... approximately 50 ng of RNA was used in a previously published qRT-PCR duplex assay (Price et al. 2010) with the TaqMan® RNA-to-Ct™ 1-step kit (Applied Biosystems™, ThermoFisher Scientific) on a QuantStudio™ 3 Real-Time PCR system (Applied Biosystems™ ...
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bioRxiv - Plant Biology 2020Quote: Young seedlings were incubated in 20 μM 5-ethynyl-2’-deoxyuridine (EdU) (Click-iT™ EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit, ThermoFisher Scientific/Invitrogen, Waltham, Massachusetts, USA) made in ddH2O for 30 min at 24 °C ...
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bioRxiv - Microbiology 2021Quote: ... The extracted DNA yield (ng DNA g-1 dry weight soil) was quantified with the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). We recognize that this calculation does not take into account inflation in DNA yield due to the growth of fungi and other organisms not accounted for in our study ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was first quality-checked by gel electrophoresis and then quantified with a PicoGreen fluorometer quantitator and Quant-iT™ PicoGreen™ dsDNA Assay Kit (Invitrogen™, ThermoFisher Scientific). The Genomic Sequencing and Analysis Facility (GSAF ...
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bioRxiv - Immunology 2020Quote: ISH was performed as reported previously (Erickson et al., 2020; Osterrieder et al., 2020) using the ViewRNA™ ISH Tissue Assay Kit (Invitrogen by Thermo Fisher Scientific) following the manufacturer’s instructions with minor adjustments ...
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bioRxiv - Genomics 2021Quote: ... Total RNA concentration was measured using Qubit® RNA HS Assay Kit on a Qubit® 2.0 Fluorometer (Thermo Fisher Scientific Inc., Waltham, MA, USA). Integrity was assessed using Agilent RNA 6000 Nano Kit on a 2100 Bioanalyzer instrument (Agilent Technologies ...
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bioRxiv - Immunology 2022Quote: We isolated T cells from PBMC of patients and healthy controls for autologous mixed lymphocyte reaction (MLR) assay using Dynabeads Untouched Human CD4+ T Cells kit (Invitrogen Dynal cat. No.113.52D, US). For MLR ...
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bioRxiv - Microbiology 2022Quote: ... Extracts were clarified by centrifugation (13,000 × g for 10 minutes) and quantified by Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). 500 μg of cell lysate were combined with 5μg of IP antibody and incubated overnight at 4°C with mixing to form the immune complex ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was transferred to a new tube and protein concentrations were assessed using a bicinchoninic acid (BCA) assay kit (Thermo Fisher Scientific; Waltham, MA, USA) and microplate spectrophotometer (Synergy H1 ...
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bioRxiv - Plant Biology 2023Quote: ... A 100 μL plant extract was used in each reaction and the H2O2 concentration was measured with the Amplex Red hydrogen peroxide/peroxidase assay kit (Thermo Fisher Scientific, Cat. No. A22188).
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bioRxiv - Immunology 2024Quote: ... The total protein concentrations in amniotic fluid (supernatant) and tissue lysate samples were tested by the Pierce BCA Protein Assay Kit (Cat# 23225; Pierce Biotechnology, Thermo-Fisher Scientific, Inc., Rockford, IL, USA) prior to immunoblotting ...
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bioRxiv - Microbiology 2024Quote: ... The concentration and purity of extracted DNA from all samples and the controls were evaluated using Invitrogen™ Quant-iT™ dsDNA Assay Kit (Thermo Fisher Scientific, USA) and a Nanodrop 2000 (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 g of each microbial mat sample and checked the concentration and quality of the DNA extracts using the Qubit dsDNA BR Assay kit (Thermo Fisher Scientific, Waltham, MA, USA). We used the Nextera XT kit (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... All the OCR and ECAR values were normalised by DNA content measured for each well using the Quant-iT™ PicoGreen™ dsDNA Assay Kit (Invitrogen™, Dublin, Ireland) as previously described by [106].
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bioRxiv - Molecular Biology 2023Quote: ... the cDNA was used for real-time PCR with three multiplex wells once with TaqMan gene assay kit (Thermo Fisher Scientific, Applied Biosystems, Carlsbad, CA, USA). The results were normalized to GAPDH ...
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bioRxiv - Immunology 2023Quote: The viral load in different tissue samples was determined as described previously (72) TaqMan™ 2019nCoV Assay Kit v1 (Thermo Fisher Scientific, Waltham, MA, USA).
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ANGPTL8 R59W variant influences inflammation through modulating NF-κB pathway under TNFα stimulationbioRxiv - Biochemistry 2023Quote: Candidate SNP genotyping was performed using TaqMan® Genotyping Assay kit on ABI 7500 Real-Time PCR System from Applied Biosystems (Foster City, CA, USA). Each polymerase chain reaction sample was composed of 10 ng of DNA ...
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bioRxiv - Biophysics 2023Quote: ... we determined the total protein concentration of each sample using a Pierce™ Microplate BCA Protein Assay Kit (Thermo Fisher Scientific, 23252, Waltham, MA, USA) to calculate the normalized collagen content.
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bioRxiv - Molecular Biology 2023Quote: ... The resultant sediment was dissolved in 40 µl of PBS and its protein concentration was estimated by a bicinchoninic acid (BCA) assay kit (23228, 1859078, Thermo Fisher Scientific, Waltham, MA, USA), using bovine serum albumin (23209 ...
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bioRxiv - Molecular Biology 2023Quote: RNA-protein pull-down assay (RNAP) was conducted in triplicates following a modified protocol adapted from Pierce™ Magnetic RNA-Protein Pull-Down Kit (Thermo Scientific™, No.: 20164). Briefly ...
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bioRxiv - Physiology 2023Quote: OPP fluorometry was performed adapting the regular instructions for the Click-iT™ Plus OPP Alexa Fluor™ 488 Protein Synthesis Assay Kit (cat #C10456; Thermo Scientific, Waltham, MA) to live myofibers ...
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bioRxiv - Biophysics 2023Quote: ... Total protein concentrations of tissue and cancer cell samples were determined using the Pierce BCA Protein Assay Kit (#RG235622, Thermo Fisher Scientific, Waltham, MA, USA). Further steps were performed as described for protein isolation from mouse organ tissues.