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Citations for Thermo Fisher :
9451 - 9500 of 10000+ citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were prepared from 10 ng gDNA using the Ion Torrent™ Oncomine™ Comprehensive Assay v3C (OCAv3C) kit (Thermo Fisher Scientific, cat# A35806) and the Ion Torrent™ Ion Chef™ instrument model 4247 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: Nascent protein synthesis was analyzed in HPMs by using the Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (Invitrogen™, cat #C10428). Click-iT® HPG (L-homopropargylglycine ...
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Identification of ABHD6 as a regulator of lysophosphatidylserines in the mammalian liver and kidneysbioRxiv - Biochemistry 2024Quote: ... The protein concentrations of the membrane and soluble proteomic fractions were measured using the Pierce BCA Protein Assay Kits (Thermo Fisher Scientific, catalog no: 23225).
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bioRxiv - Microbiology 2024Quote: ... according to the manufacturer’s instructions and DNA concentration in extracts was measured with Qubit™ dsDNA High Sensitivity (HS) assay kits and Qubit 2.0 Fluorometer (Thermo Fisher Scientific, Waltham Massachusetts, US). RNA was extracted using the RNeasy kit (Qiagen ...
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Identification of ABHD6 as a regulator of lysophosphatidylserines in the mammalian liver and kidneysbioRxiv - Biochemistry 2024Quote: ... The protein concentrations of the membrane and soluble proteomic fractions were measured using the Pierce BCA Protein Assay Kits (Thermo Fisher Scientific, catalog no: 23225).
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bioRxiv - Molecular Biology 2020Quote: Global translation assay was performed using Click-iT® AHA Alexa Fluor® 488 Protein Synthesis HCS Assay (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Genetics 2020Quote: ... 12.5 μL of TaqMan Gene Expression Master Mix and 1.25 μL of primers and probes (TaqMan® gene expression assay, assay ID: Rasa3, MM00436272.M1; Applied Biosystems). Samples were prepared in triplicate ...
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bioRxiv - Bioengineering 2021Quote: ... qRT-PCR assays were performed as TaqMan single-plex assays on the StepOne Plus Real-Time PCR System (Thermo Fisher Scientific), using the manufacturer recommended settings for quantitative and relative expression ...
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bioRxiv - Biochemistry 2020Quote: ... the purified LC/A variants were subjected to BCA assay to quantify protein concentration through comparison to BSA standards (Pierce™ BCA Assay, ThermoFisher). The proteins were stored at 4 °C before assays.
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bioRxiv - Pharmacology and Toxicology 2020Quote: The copy number variations (CNVs) in the CYP2D6 gene were determined with the TaqMan® Copy Number Assay (Assay ID: Hs00010001_cn; Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble supernatant was collected and protein concentration was quantified via bicinchoninic assay (BCA) assay (Pierce Thermo Scientific, Rockford, IL, USA). Normalized soluble supernatant or 20 μL of conditioned media was run on a 4-20% Tris-Gly SDS-PAGE gel (Life Technologies ...
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bioRxiv - Physiology 2021Quote: ... The supernatant was collected and protein concentration was quantified via a bicinchoninic assay (BCA) assay (Pierce Thermo Scientific, Rockford, IL, USA). Approximately 30 μg of soluble supernatant was run on a 4-20% Tris-Gly SDS PAGE gel (Life Technologies ...
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bioRxiv - Systems Biology 2019Quote: ... and protein concentration in each sample was determined by Bradford assay using Pierce™ Coomassie Plus Assay Reagent (Thermo Fisher Scientific). Subsequently ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The assay procedure is based on the manufacturer’s protocol for LanthaScreen™ TR-FRET Thyroid Receptor beta Coactivator Assay (Invitrogen, PV4686) with slight ...
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bioRxiv - Genetics 2021Quote: ... and DNA pulled down using an anti-AHR antibody from melanocytes heterozygous for rs117132860 (c87, c197 and c262) was used as template DNA for a Taqman genotyping assay (Assay ID: ANU699F, Thermo Scientific). All experiments were performed for at least three biological replicates.
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bioRxiv - Molecular Biology 2024Quote: ... proteins were quantified with the Bradford Assay following a standard protocol (Coomassie Plus—The Better Bradford Assay™ Reagent, Thermo Scientific), and equal amounts of solubilized proteins were heated in Laemmli sample buffer (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... gene for the internal normalization primers-probe (VIC) set (VIC-MGB: assay ID Hs03405707_g1; TaqMan Gene Expression Assay, Thermo Fisher Scientific). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... s3077 and s3078) and IKKγ (Cat# 4390824, assay ID: s16186 and Cat # 4392420, assay ID: s533388) were obtained from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... protein concentrations were determined by Bradford Protein Assay protein using Coomassie protein assay reagent (Thermo Fisher Scientific-Invitrogen, Waltham, MA, USA). 5 µg of each protein fraction was added to 1x loading buffer (250 mM Tris pH 6.8 ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR was performed using Applied Biosystems custom TaqMan Gene Expression Assays designed using the Taqman Assay Search Tool (Life Technologies) for the ubiquitous CDC42 isoform ...
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bioRxiv - Immunology 2023Quote: ... coli BioParticles™ Conjugate and DQ™-BSA Red (used in 96 well plate assay) and DQ™-BSA -Green (used for live cell assay) was purchased from Invitrogen.
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bioRxiv - Cancer Biology 2023Quote: ... IL-1β and Gapdh gene expression in mouse bone marrow populations were quantified by TaqMan gene expression assay (Assay ID: and; ThermoFisher Scientific). Each sample was run in triplicates using 2-5 ng cDNA in a 384 well plate and the qPCR was performed using VIIA 7 real time PCR instrument from Applied Biosystems.
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bioRxiv - Biophysics 2024Quote: ... or ∼5 mg/mL (NFL, NFH, NFH Δ36-72, NFH Δ462-638) by BCA assay (Pierce BCA Protein Assay, Thermo Scientific). Protein aliquots were stored at -80 C.
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bioRxiv - Molecular Biology 2020Quote: ... Supernatants were then transferred into fresh Eppendorfs and frozen at −80°C (for radiolabeled glucose to protein) or their protein concentrations calculated (Western blot) using a Pierce BCA Protein Assay kit (Thermo Scientific, Cat#23225, Waltham, MA, USA).
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bioRxiv - Molecular Biology 2019Quote: ... the concentration of the in vitro transcribed antisense ssRNA control was determined by using the Qubit™ RNA BR Assay Kit (catalog-no. Q10210, Invitrogen™, Thermo Fisher Scientific Waltham, Massachusetts) and Qubit® Fluorometer 2.0 as ∼218 ng/μl.
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bioRxiv - Cancer Biology 2020Quote: The autophagic flux assay was carried out using the Premo™ Autophagy Tandem Sensor RFP-GFP-LC3 Kit (P36239, Molecular Probes, Thermo Fisher Scientific, Bremen, Germany), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: Due to the lack of aromatic amino acids within the small protein His6-sP26 the Pierce™ BCA protein assay kit (Thermo Fisher Scientific™, Waltham, USA) was used to determine the concentration of all proteins and was performed according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... The purity of recombinant proteins was analyzed by 10% Tris/Tricine SDS-PAGE and silver staining techniques and the protein concentrations were determined by employing the Pierce™ BCA protein assay kit (Thermo Fisher Scientific, Rockford, IL, USA).
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bioRxiv - Biophysics 2019Quote: Samples that were collected just after lysis were measured for the total protein concentration using a BCA assay kit (Thermo Fisher Scientific, Pierce, Rockford, IL USA). Both the biotinylated and intracellular fractions were normalized to total protein and loaded on a 4-12% Bis-Tris pre-cast gel (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2020Quote: ... approximately 50 ng of RNA was used in a previously published qRT-PCR duplex assay (Price et al. 2010) with the TaqMan® RNA-to-Ct™ 1-step kit (Applied Biosystems™, ThermoFisher Scientific) on a QuantStudio™ 3 Real-Time PCR system (Applied Biosystems™ ...
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bioRxiv - Microbiology 2021Quote: ... DNA was isolated by overnight precipitation in ethanol and DNA concentration was determined with the Qubit™ dsDNA HS Assay Kit (Thermo Fisher Science, Waltham, MA, USA). Sequencing libraries were generated using the TruSeq ChIP Library Preparation Kit (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... the DNA extracted from the seed samples and the positive and negative controls were quantified using the Qubit™dsDNA BR Assay Kit (ThermoFisher Scientific, Waltham, MA, United States). Then ...
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Horizontal gene transfer facilitates the spread of extracellular antibiotic resistance genes in soilbioRxiv - Microbiology 2021Quote: ... The final concentrations of eDNA ranged from 25-50 ng and were appropriately diluted for each experiment (determined using Qubit fluorometric quantification and Invitrogen Quant-iT PicoGreen dsDNA Assay Kit). P ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were then cultured with the indicated compound for a total of 6-days before cell lysis and quantification of proliferation using the Quant-iT™ PicoGreen dsDNA Assay Kit (ThermoFisher Scientific, Grand Island, NY, USA). For long-term proliferation (12-days) ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were passaged and an equal number of cells per treatment were seeded into a 96-well plate and were cultured with the indicated compounds for 3 more days (total 12-days of treatment) before cell lysis and quantification of proliferation using the Quant-iT™ PicoGreen dsDNA Assay Kit (ThermoFisher Scientific, Grand Island, NY, USA).
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bioRxiv - Genomics 2024Quote: ... the concentration and size of the digested sample was examined by the Qubit® Fluorometer and Qubit™ dsDNA HS and BR Assay Kits (Invitrogen™ Cat. No. Q32851), and the TapeStation D5000 HS ScreenTape ...
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bioRxiv - Genomics 2024Quote: ... The sample was then proceeded to quality control by quantifying with the Qubit® Fluorometer and Qubit™ dsDNA HS and BR Assay Kits (Invitrogen™ Cat. No. Q32851). An overnight pulse-field gel electrophoresis was performed to estimate the size of the isolated DNA ...
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bioRxiv - Genomics 2024Quote: ... The size and concentration of the library were assessed with the pulse-field gel electrophoresis and the Qubit® Fluorometer and Qubit™ dsDNA HS and BR Assay Kits (Invitrogen™ Cat. No. Q32851), respectively ...
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bioRxiv - Genomics 2024Quote: ... The concentration and size of the library were examined with the Qubit® Fluorometer and Qubit™ dsDNA HS and BR Assay Kits (Invitrogen™ Cat. No. Q32851), and an overnight pulse-field gel electrophoresis ...
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bioRxiv - Genomics 2024Quote: ... the concentration and fragment size of the digested sample was validated by the Qubit® Fluorometer and Qubit™ dsDNA HS and BR Assay Kits (Invitrogen™ Cat. No. Q32851), and the TapeStation D5000 HS ScreenTape ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were stored at −80 °C or directly used for the quantification of total protein concentrations with the Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, United States). Thereafter ...
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bioRxiv - Plant Biology 2023Quote: The RNA was measured with Qubit® RNA HS Assay Kits again and libraries were prepared with NEBNext® Ultra™ II RNA Library Prep with Sample Purification Beads (Invitrogen Life Technologies (Ambion Applied Biosystem), E7775S ...
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bioRxiv - Neuroscience 2023Quote: Total protein levels in cortex homogenates were quantified using a Pierce Microplate BCA Protein Assay Kit (Thermo Scientific Pierce Protein Biology, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... the obtained genomic libraries were purified with Agencourt AMPure XP and subjected to quantity and quality determination using a Qubit 3 Fluorometer with a Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and an Agilent 2100 BioAnalyzer with a high-sensitivity DNA chip (Agilent Technologies ...
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bioRxiv - Genomics 2024Quote: ... The concentration and size of the library were examined using the Qubit® Fluorometer and Qubit™ dsDNA HS and BR Assay Kits (Invitrogen™ Cat. No. Q32851), and the pulse-field gel electrophoresis ...
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bioRxiv - Genomics 2024Quote: ... the concentration and fragment size of the digested sample was checked by the Qubit® Fluorometer and Qubit™ dsDNA HS and BR Assay Kits (Invitrogen™ Cat. No. Q32851), and the TapeStation D5000 HS ScreenTape ...
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bioRxiv - Neuroscience 2024Quote: ... 10 ng of miRNA underwent reverse transcription to cDNA using the Taqman™□ MicroRNA Reverse Transcription Kit and the Taqman™□ MicroRNA Assay (miR-200a-3p: 000502, and U6 snRNA: 001973; Thermo Fisher Scientific Inc., USA). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: Total protein levels were quantified in tissue homogenates in triplicates using the Pierce Microplate BCA Protein Assay Kit (Thermo Scientific Pierce Protein Biology, Thermo Fisher Scientific, Waltham, MA, USA). Individual cytokine levels are reported as pg/mg of protein in each sample.
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations in the supernatants were measured using the bicinchoninic acid assay (Pierce BCA Protein Assay Reagent, Thermo Scientific, Waltham, MA, USA). Equal amount of protein lysates (40 mg ...
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bioRxiv - Genetics 2020Quote: ... C_11904666_10] and rs5443 [assay ID: C_2184734_10]) were genotyped using TaqMan® Genotyping Assays following manufacturer protocols (Thermo Fisher Scientific Inc, Waltham, MA). Genotypes for all SNPs were determined to be in Hardy-Weinberg equilibrium through testing using the Hardy-Weinberg equilibrium calculator tool [47].