Labshake search
Citations for Thermo Fisher :
901 - 950 of 10000+ citations for Zika Virus NS1 Protein Uganda strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... Viral plaques were passaged at an MOI of ∼0.001 in MDCK-II cells in virus growth medium comprised of Opti-MEM (Gibco), 2.5 mg/ml bovine serum albumin (Sigma-Aldrich) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells infected with pLENTI-Luciferase-expressing virus (generous gift of Charlotte Kuperwasser, Tufts University) were selected with neomycin (G418, 500μg/mL, Gibco) for 2-3 weeks ...
-
bioRxiv - Biophysics 2023Quote: ... Negatively stained virus particles were observed on a 30 kV scanning electron microscope (Thermo Fisher Scientific, Quanta FEG 650) in scanning transmission electron microscopy (STEM ...
-
bioRxiv - Cell Biology 2023Quote: ... HT914 and HT915 iPSC lines) using the integration-free CytoTune 2.0 Sendai virus reprogramming kit (A16517, Thermo Fisher Scientific) as previously reported [43–45] ...
-
The direction of local adaptation on pathogen exploitation rates reverses with environmental contextbioRxiv - Evolutionary Biology 2023Quote: ... we extract virus from the pooled samples from each assay grid using a sterile pellet pestle (Fisher Scientific, U.S.A.) to manually burst larvae and release viral occlusion bodies ...
-
bioRxiv - Microbiology 2023Quote: ... The RT-PCR was performed using the Taqman Fast Virus 1-step master mix (Applied Biosystems, Foster City, CA) on a QuantStudio3 (ThermoFisher) ...
-
bioRxiv - Neuroscience 2024Quote: ... Virus was removed after 24 h and cells were cultured in fibroblast medium with 10% FBS (Gibco, 16000-044) in Dulbecco’s modified Eagle medium (DMEM ...
-
bioRxiv - Molecular Biology 2023Quote: ... MED1-IDR containing plasmid was obtained from Addgene #145276 and inserted into pcDNATM6.2-C-EmGFP-DEST supplemented with the Simian Virus 40 NLS sequence by Gateway strategy (Invitrogen) using primers described in Suppl ...
-
bioRxiv - Microbiology 2023Quote: RNA was extracted from the supernatant of MDCK cells infected with the PB2 TRAP V5 virus using Trizol (Invitrogen). RNA was reverse-transcribed with Superscript III (Invitrogen ...
-
bioRxiv - Microbiology 2023Quote: ... MRC-University of Glasgow Centre for Virus Research) cells were grown in Dulbecco’s Modified Eagle Medium (DMEM; ThermoFisher, 10566016) supplemented with 10 % fetal bovine serum (FBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... RNA was reverse-transcribed using Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase (RT) according to the manufacturer’s instructions (Invitrogen, Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2023Quote: ... were infected with HSV-1 at MOI 0.01 by replacing cell media with virus diluted in minimal media Opti-MEM (ThermoFisher). The virus was allowed to adhere to cells at 37 °C in 5% CO2 for 1 hour with minimal agitation every 15 minutes ...
-
bioRxiv - Bioengineering 2024Quote: The virus riboswitch (vRibo) RNAs were synthesized using a TranscriptAid T7 High Yield Transcription Kit (Thermo Scientific Cat# K0441) from the DNA templates generated by PCR ...
-
bioRxiv - Microbiology 2024Quote: ... The first strand cDNA was obtained using oligo(dT) primers and Moloney murine leukaemia virus-reverse transcriptase (Life Technologies), using 100 ng of purified RNA ...
-
bioRxiv - Cell Biology 2024Quote: ... Virus was removed 16-20 hours following transduction with fresh neural media [Neurobasal medium (NBM; Life Technologies, #21103-049) supplemented with 1x N-2 supplement 1x B-27 supplement ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Batches of ten 8-10 weeks old female BALB/c mice were inoculated intraperitoneally (i.p) with 105 PFU of Addavax-adjuvant recombinant virus in 200 μL of phosphate-buffered saline (PBS; Gibco). At twenty-one days post immunization ...
-
bioRxiv - Biochemistry 2024Quote: ... were infected with the virus at a cell density of 4.0 × 106 cells per milliliter in Sf900 II medium (Gibco), and grown for 48 h at 27 °C ...
-
bioRxiv - Microbiology 2024Quote: ... A virus growth medium consisting of infection media supplemented with an additional 1 µg/mL trypsin-TPCK (ThermoFisher, USA) was added to the cells ...
-
bioRxiv - Cell Biology 2024Quote: ... The supernatant was discarded and virus pellet was resuspended in the same volume of RIPA buffer (Cat #89901, ThermoFisher, 20 mM Tris-HCl ...
-
bioRxiv - Genomics 2020Quote: ... and Protein A or Protein G Dynabeads (Invitrogen). After incubation ...
-
bioRxiv - Neuroscience 2019Quote: ... Protein reagent (Thermo Scientific PierceTM BCA Protein assay) was added to the supernatant and the standards and incubated for 30 min at 37°C ...
-
bioRxiv - Immunology 2022Quote: ... or with Dynabeads protein A/protein G (Thermofisher) combined with Abatacept ...
-
bioRxiv - Biochemistry 2021Quote: ... protein marker (NativeMark Unstained protein standard, LC0725, ThermoFisher) was diluted 50x in the working buffer (40 mM HEPES pH 7.5) ...
-
bioRxiv - Immunology 2020Quote: ... protein A and protein G magnetic beads (Invitrogen) were added to capture the antibody-chromatin complexes ...
-
bioRxiv - Immunology 2021Quote: ... For protein purification protein G dynabeads (Thermo Fisher), coupled with 2.5 µg of antibody (Table S8 ...
-
bioRxiv - Neuroscience 2022Quote: ... Magentic dynabeads Protein G or Protein A (Invitrogen) were washed with 0.05% Triton X-100 in PBS ...
-
bioRxiv - Developmental Biology 2022Quote: ... Protein A and Protein G Dynabeads (Thermofisher Scientific) were washed twice with IP dilution buffer (150 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Plant Biology 2022Quote: ... Magnetic Protein A and Protein G Dynabeads (Invitrogen) were added and incubated at 4°C for two hours ...
-
bioRxiv - Plant Biology 2022Quote: ... Magnetic Protein A and Protein G Dynabeads (Invitrogen) were added and incubated at 4 °C for 2 hours ...
-
bioRxiv - Microbiology 2023Quote: ... protein concentrations were determined by Bradford Protein Assay protein using Coomassie protein assay reagent (Thermo Fisher Scientific-Invitrogen, Waltham, MA, USA). 5 µg of each protein fraction was added to 1x loading buffer (250 mM Tris pH 6.8 ...
-
bioRxiv - Neuroscience 2020Quote: Total protein concentrations of isolated protein lysates were quantified by BCA protein assay kit (ThermoFisher) and equal protein amounts were boiled at 95°C for 10 minutes ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Total protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific). Filter-aided sample preparation (FASP ...
-
bioRxiv - Biophysics 2021Quote: ... The GFP-tagged and the knockout strains come from the commercial Yeast GFP clone collection (ThermoFisher Scientific). All cultures were grown in YPD medium (1% Yeast Extract ...
-
bioRxiv - Microbiology 2021Quote: ... perRB (M1474) and double perRAperRB mutant strains were harvested and resuspended in 1 ml TRIzol (ThermoFisher Scientific) and stored at -80°C ...
-
bioRxiv - Molecular Biology 2021Quote: Cells from each strain and condition were first resuspended in 0.5 ml TRI reagent each (ThermoFisher, AM9738). One aliquot of frozen S ...
-
bioRxiv - Microbiology 2019Quote: ... The strain was also incubated under anaerobic conditions in a Forma Anaerobic System Glovebox (Thermo Scientific, 1025). The growth pattern of the isolate was also determined on Salmonella-Shigella Agar plate (Himedia ...
-
bioRxiv - Biophysics 2021Quote: ... The plasmid construct was transformed into an electrocompetent strain of Escherichia coli DH10β cells (ThermoFisher Scientific, USA) via electroporation ...
-
bioRxiv - Biochemistry 2021Quote: The DNA sequence encoding the TBEV NS2B-NS3pro enzyme (strain Hypr; GeneBank: KP716978.1) was commercially synthesized (Invitrogen) and encoded NS2B (residues 45-96) ...
-
bioRxiv - Microbiology 2022Quote: ... pneumoniae serotype 2 strain D39 [17] was plated on brain heart infusion (BHI, Thermo Scientific, MA, USA) agar plates supplemented with 3% v/v defibrinated horse blood (Thermo Scientific ...
-
bioRxiv - Cell Biology 2022Quote: ... All HTM cell strains were validated with dexamethasone-induced (DEX; Fisher Scientific, Waltham, MA, USA; 100 nM) myocilin expression in more than 50% of cells by immunocytochemistry and immunoblot analyses (Suppl ...
-
bioRxiv - Genetics 2022Quote: ... both strains were harvested at an OD600 of 0.3 pelleted and immediately suspended in RNAlater (Thermo Fisher) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: The H99S strain [21] of Cn serotype A was grown in yeast peptone dextrose (YPD; ThermoFisher Scientific) broth ...
-
bioRxiv - Biochemistry 2019Quote: ... smegmatis wild-type or Rv1626D65E over-expression strain was used for DNase I (AM2238, Thermo Fisher Scientific) treatment ...
-
bioRxiv - Synthetic Biology 2020Quote: Promastigote-form of Leishmania donovani (Ag83 strain) was cultured at 26 *C in M199 media (GIBCO, India) pH 7.4 supplemented with 10% (v/v ...
-
bioRxiv - Biochemistry 2020Quote: ... were expressed in the bacterial Escherichia coli (E. coli) BL21(DE3) pLysS strain (Invitrogen, Thermo Fisher Scientific) as described in 38 ...
-
bioRxiv - Microbiology 2020Quote: Approximately 2.8 × 107 cells/ml of Escherichia coli strain DH10B harboring a pBAD vector (Thermofisher Scientific, Canada), suspended in a total of 50 ml 1× PBS ...
-
bioRxiv - Biochemistry 2020Quote: ... were expressed in the bacterial Escherichia coli (E. coli) BL21(DE3) pLysS strain (Invitrogen, Thermo Fisher Scientific) as described in 38 ...
-
bioRxiv - Immunology 2021Quote: ... aeruginosa (strain PA14) and clinical isolates were grown in lysogeny broth (LB, Thermo Fisher Scientific, Waltham, MA) liquid cultures at 37°C with shaking at 225 rpm ...
-
bioRxiv - Molecular Biology 2022Quote: ... Yeast strains used in Tables S1-S2-S3 were taken from the heterozygote yeast deletion collection (ThermoFisher). For nicotinamide (NAM ...
-
bioRxiv - Microbiology 2022Quote: Spn serotype 4 strain TIGR4 was grown overnight on 1% agar- Todd Hewitt Broth (Thermo Fisher Scientific) with yeast (THY ...