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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for Zika Virus NS1 Protein Uganda strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The genomic titer of each virus was determined by quantitative PCR using the ABI 7700 (Applied Biosystems) and primers specific to the WPRE ...
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bioRxiv - Immunology 2020Quote: ... Virus detection was performed using One-Step RT-PCR SuperScript(tm) III Platinum(tm) Kit (Life Technologies). Thermal cycling was achieved at 55°C for 10 minutes for reverse transcription ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293T cells (ATCC) were cultured for virus production in Dulbecco’s Modified Eagle Medium: Nutrient Mixture (DMEM) (GIBCO) supplemented with 10% FBS and 1% P/S ...
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bioRxiv - Microbiology 2022Quote: ... and plasmids encoding two vaccinia virus capping enzyme subunits (pCAG-D1R and pCAG-D12L −0.8μg each) using 16μL Lipofectamine 2000 (Invitrogen) per transfection reaction in a total volume of 200μL of Opti-MEM (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... Real-time RT-PCR analysis was performed using Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... iPSC lines were generated using the Sendai virus (CytoTuneTM-iPS 2.0 Sendai Reprogramming Kit, Thermo Fisher Scientific) carrying the Yamanaka reprogramming factors OCT3/4 ...
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bioRxiv - Neuroscience 2023Quote: ... medium containing virus was processed by centrifugation (Sorvall WX-90 Ultracentrifuge and SureSpin 630 rotor; ThermoFisher Scientific), using a sorbitol cushion (20% sorbitol ...
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bioRxiv - Neuroscience 2023Quote: Expanded PBMCs were transduced Sendai virus reprogramming vector – according to CytoTune® 2.0 Sendai (Thermo Fisher Scientific). Before cells infection ...
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bioRxiv - Molecular Biology 2023Quote: ... purified gRNA from benzonase-treated virus material was treated with DNaseI (Thermo Fisher Scientific, Waltham, MA, USA), and analyzed using an Agilent 2100 bioanalyzer (Agilent Technologies ...
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bioRxiv - Genetics 2023Quote: ... The PBMCs were reprogrammed by using Sendai virus (CytoTuneTM-iPS 2.0 Sendai Reprogramming kit, Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The following primary antibodies were used: influenza A virus nucleoprotein (NP, PA5-32242, 1:3000, ThermoFisher Scientific), XPO1 (46249S ...
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bioRxiv - Molecular Biology 2023Quote: ... Isolated ZIKV RNA was titrated by qRT-PCR using TaqMan Fast virus 1-step master mix (ThermoFisher) and a LightCycler 480 or LC96 instrument (Roche) ...
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bioRxiv - Bioengineering 2023Quote: ... reverse transcription was performed with random hexamers and Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Invitrogen). Synthesized RNA was used as a standard (BEI) ...
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bioRxiv - Microbiology 2023Quote: ... Infectious virus was reconstituted by transfecting ARPE-19 cells with BAC DNA and Lipofectamine 3000 (ThermoFisher L3000001); high titer stocks generated after three passages in ARPE-19 cells were used for this project ...
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bioRxiv - Microbiology 2023Quote: ... infected cells were stained with primary antibody (Vaccinia Virus Polyclonal FITC Antibody ThermoFisher PA1-73191, 1:1000) and Hoechst 33342 (1:10,000) ...
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bioRxiv - Biochemistry 2023Quote: ... virus was harvested by collecting supernatant and filtering it through 0.45µm PES sterile filter (Thermo Fisher, 2954545). Virus was concentrated by ultracentrifugation with a 20% sucrose cushion ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed twice with PBS and then incubated with virus suspended in infectious medium (DMEM; Invitrogen) supplemented with 35% bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2023Quote: ... This virus mixture was used to infect 1.8–2.4 liters of High Five cells (Thermo Fisher Scientific) at a cell density of 3 × 106 per ml for 60 h at 27°C ...
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bioRxiv - Cell Biology 2023Quote: ... from a cDNA library of HCT116 which was prepared using Moloney murine leukemia virus reverse transcriptase (Invitrogen), as described previously (Ninagawa et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293T cells used for the production of virus were cultured in DMEM supplemented with 2mM glutamine (Gibco), 10% Tetracycline-free FBS (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... The BA.1 and BA.2 supernatants were additionally concentrated using the Intact Virus Precipitation Reagent (Invitrogen). All viral stocks were stored at -80°C until use ...
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Resolution of SARS-CoV-2 infection in human lung tissues is driven by extravascular CD163+ monocytesbioRxiv - Immunology 2024Quote: ... cells were infected with virus diluted in 10 mL of Opti-MEM (ThermoFisher Scientific, Waltham, MA, USA) and incubated for 1 h at 37°C to allow for virus adsorption ...
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bioRxiv - Microbiology 2024Quote: ... and the virus was incubated with 25 μg of Alexa Fluor 488 NHS Ester (succinimidyl ester; Invitrogen) for 1 hour at 25°C ...
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bioRxiv - Microbiology 2024Quote: ... half of the virus supernatant after the concentration step was digested with amplification grade DNAse I (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was extracted from 1010 virus particles using the PureLink TM Genomic DNA mini kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... protein concentrations were determined by Bradford Protein Assay protein using Coomassie protein assay reagent (Thermo Fisher Scientific-Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Cell proteins and EVs proteins were quantified by Qubit Protein Assay (Invitrogen) using a Qubit 3.0 Fluorometer (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... Vibrio parahaemolyticus strains were grown either in lysogeny broth (LB) (Fisher Scientific, Fair Lawn, NJ) with 3% (wt/vol ...
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bioRxiv - Microbiology 2020Quote: ... pylori were constructed and amplified using Escherichia coli one-shot top10 or DH5α strains (Thermofisher). H ...
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bioRxiv - Physiology 2019Quote: ... coli bioparticles at 250 µg/ml (strain K-12; Vybrant phagocytosis assay kit; ThermoFisher Scientific) in phenol red-free ...
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bioRxiv - Microbiology 2019Quote: ... DNA was prepared from each strain using a Purelink genomic DNA mini kit (Invitrogen, #K1820). DNA concentrations were quantified using Qubit and normalized to 0.5 ng/uL ...
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bioRxiv - Biochemistry 2019Quote: ... The strains were then grown at 30°C in a shaking incubator (Thermo scientific, USA), with 200 rpm ...
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bioRxiv - Genetics 2020Quote: ... All strains were maintained on Nematode Growth Media (NGM) plates with 50μg/mL streptomycin (Gibco) and a lawn of OP50-1 bacteria (a gift from M.-W ...
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bioRxiv - Evolutionary Biology 2020Quote: The Escherichia coli strains DH5α (Bethesda Research Laboratories) and TOP10 (Life Technologies, Carlsbad, CA, USA) were used for the cloning and amplification of plasmids ...
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bioRxiv - Systems Biology 2020Quote: ... aureus strain NCTC 8325 (DSM 4910) grown in Tryptone Soy Broth (TSB; Thermo Fisher Scientific) at 37°C were diluted to an OD600 of 0.05 ...
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bioRxiv - Molecular Biology 2020Quote: ... phaffii KM71 strain (referred to as ΔAOX1 in this study) was purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Vectors for DiCre strain generation were generated as previously described45 using Gateway Assembly (Thermo Fisher). PCR amplicons were resolved in 1% agarose (Melford ...
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bioRxiv - Microbiology 2021Quote: ... RKL9 and PfKelch13R539T strains were maintained at 2% hematocrit in RPMI 1640 medium (Invitrogen, USA) supplemented with 27.2 mgL-1 hypoxanthine and 0.5% Albumax I ...
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bioRxiv - Systems Biology 2022Quote: ... the JM109 strain was transformed with 1 μg of the pBlueScript II SK+ plasmid (Invitrogen), which harbors an ampicillin resistance gene as a selection marker ...
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bioRxiv - Plant Biology 2022Quote: ... pastoris strain X33 using the EasySelect Pichia expression kit (Invitrogen Life Technologies, cat K1740-01) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... pastoris strain X33 using the EasySelect Pichia expression kit (Invitrogen Life Technologies, cat K1740-01) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Transformed strains cells were stained with 100 nM Mitotracker (CMTMRos MitoTracker Orange, #M7510 Thermo Fisher) to visualize mitochondrial compartment ...
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bioRxiv - Microbiology 2022Quote: ... The isolated STEC 150229 strain was routinely grown in LB broth (Affymetrix USB, CA, USA) at 37°C for 48 h ...
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bioRxiv - Synthetic Biology 2020Quote: ... Yeast strains were grown in the yeast media “BD Difco™ YPD Broth (Fisher Scientific) plus 60 mg of adenine per liter,” “c7112 ...
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bioRxiv - Genomics 2019Quote: ... RNA was isolated in triplicate for each mutant strain by the TRIzol reagent protocol (Invitrogen) followed by miRNeasy mini column (Qiagen) ...
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bioRxiv - Microbiology 2019Quote: Neuraminidase (NA) and haemagglutinin (HA) genes of the following strains were synthesised by GeneArt (Invitrogen) and cloned into a pHW2000 vector [68] ...
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bioRxiv - Cell Biology 2019Quote: ... Commercially-available control fibroblast strains derived from healthy adults or children were obtained from ThermoFisher Scientific or the Coriell Institute ...
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bioRxiv - Cell Biology 2020Quote: ... Ligations were transformed into the STBL3 Escherichia coli strain (Thermo Fisher, Cat No. C7373-03) and positive clones were identified by Sanger sequencing (Genewiz) ...
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bioRxiv - Microbiology 2019Quote: ... rpvA mutant and the complemented strain were washed twice and resuspended in RPMI1640 (Life Technologies) and the bacteria diluted 1:1000 in 1 mL RPMI1640-10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... discoideum strains (bsr) were grown in HL5 media supplemented with 4 µg/ml Blasticidin (ThermoFisher). The creation of the cadA-mutant strain was described previously (16).