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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for Human Procollagen II C Terminal Propeptide PIICP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... TaqMan MicroRNA RT kit and TaqMan Universal PCR master Mix II no UNG (all from Applied Biosystems). miRNA levels were quantified by using standard curves generated from serially diluted synthetic miRNAs ...
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bioRxiv - Molecular Biology 2020Quote: cDNA was isolated from 250,000 cells per condition using the Cells-to-cDNA II Kit (Ambion AM1722) following the manufacturer’s two-step RT-PCR protocol ...
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bioRxiv - Neuroscience 2022Quote: ... miRNA plasmids were prepared using the BLOCK-iT Pol II miR RNAi Expression Vector Kit (ThermoFisher Scientific) with top and bottom sequences targeting mouse Rxra cDNA (Top ...
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bioRxiv - Synthetic Biology 2020Quote: Two- to six-part DNA assemblies were performed using GeneArt Type IIs (BsaI) assembly kit (Invitrogen, CA). Specifically ...
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bioRxiv - Microbiology 2021Quote: ... Probes were labeled using [α-32P]-dCTP and the DECAprime II DNA labeling kit (Thermo Fisher Scientific). Both hybridization and detection of the membranes were carried out as described in the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... Purified PCR products were included into the PCR-II TOPO vector using TOPO-TA cloning kit (InVitrogen), and clones containing CXCL12 sequence with BstbI and PmlI restriction sites respectively in 5’ and 3’ ends of the coding sequence were selected ...
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bioRxiv - Plant Biology 2022Quote: ... The total RNA was amplified twice with the MessageAmp II aRNA Amplification kit (Thermo Fisher Scientific, USA).
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bioRxiv - Microbiology 2021Quote: ... All toxins and chimeras were expressed and purified with cleavable N-terminal 6His-SUMO fusions (ThermoFisher), grown to an OD600 of 0.8 in LB media ...
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bioRxiv - Cell Biology 2020Quote: ... the N-terminal His6-tag was removed by the addition of His6-rTEV protease (Life Technologies) and dialyzed in H Buffer for 36 h at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Then multiple deoxycytidines were added to the 3’ end of cDNA by terminal deoxynucleotidyl transferase (ThermoFisher). The gene specific primers and universal 5’ end anchor primer (5’ AP ...
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bioRxiv - Molecular Biology 2023Quote: ... 8 μm thickness coronal sections were putted in 1x terminal deoxynucleotidyl transferase buffer (Invitrogen, Carlsbad, CA) for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... 3’ terminal of the cDNA was enriched using Dynabeads® MyOne Streptavidin C1 beads (Invitrogen, 65001). The RNAseq libraries were constructed using the Kapa Hyper Prep Kit (Kapa Biosystem ...
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bioRxiv - Biochemistry 2024Quote: ... and inserted into a pET-SUMO expression vector containing an N-terminal hexahistidine-SUMO tag (Invitrogen). PARP1 truncations (PARP1-ZnF ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 10 μL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Neuroscience 2024Quote: ... and c- MYC using the CTSTM CytoTuneTM-iPS 2.1 Sendai Reprogramming Kit (Thermo Fisher). The manufacturer’s instructions were strictly followed (CTSTM CytoTuneTM-iPS 2.1 Sendai Reprogramming Kit User Guide).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cDNA plasmids in pcDNA™4/TO Mammalian Expression Vector were transiently transfected into Expi293F™ cells stably expressing human FHF2b and human SCN1B subunit (polyclonal) background using ExpiFectamine™ 293 Transfection Kits (Gibco,Thermo Fisher Scientific CAT #: A14524). Induction was achieved using Tetracycline (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... Hs05441121_cn (human) (ThermoFisher). This line has been submitted to the European Mouse Mutant Archive (EMMA ...
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bioRxiv - Neuroscience 2021Quote: ... Hs06560655 (human) (ThermoFisher).
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bioRxiv - Cell Biology 2024Quote: ... Human fibronectin (Invitrogen) was added to stamps at a concentration of 40 µg/ml for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... [72] at 4 °C overnight followed by staining with 2 µg/ml of secondary AlexaFluor647-conjugated Donkey anti-human IgG antibody (ThermoFisher Scientific, OR, USA) at room temperature for 45 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells stably expressing human recombinant LOXL1 were maintained in culture (37°C, 5% CO2) at log-phase growth in advanced DMEM (Thermo Fisher Scientific, 12491015) with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2023Quote: Full-length human MRS2 (Uniprot Q9HD23) conjugated with a FLAG tag at the C-terminus was expressed in Expi293F cells (Thermo Fisher Scientific, A14527). Cells at a density of 2.0-3.0 × 106 cells/ml were transfected with the MRS2-encoding pCMV plasmid using polyethylenimine (PEI ...
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bioRxiv - Cell Biology 2023Quote: ... Descemet’s membrane with adherent endothelial cells were stripped from the corneoscleral buttons and incubated overnight at 37°C in minimal medium (Human Endothelial SFM, Gibco, cat# 11111-044); 2% charcoal stripped fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2022Quote: ... Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (#C10428) and 1-Step Human Coupled IVT Kit – DNA (#88882) were obtained from Thermo Fisher Scientific Inc. ...
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bioRxiv - Cell Biology 2023Quote: Luminex assays were performed using the following kits according to the manufacturer’s instructions: hCG Human ProcartaPlex Simplex Kit (Invitrogen, EPX010-12388-901), Bio-Plex Pro Human Inflammation Panel 1 IL-28A / IFN-λ2 (Bio-rad ...
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bioRxiv - Microbiology 2020Quote: ... Lung or liver total RNA was extracted with the Nucleospin RNA II kit (Macherey Nagel, Düren, Germany) and reverse-transcribed with the High-Capacity cDNA Archive Kit (Applied Biosystems, Foster City, CA, USA). The cDNA was amplified using SYBR-Green-based real-time PCR on a Quantstudio™ 12K Real-Time PCR System Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... S97A mutation was inserted using QuickChange II site directed mutagenesis II from Thermo Fisher.
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bioRxiv - Cell Biology 2023Quote: ... NEFL cDNAs were made using SuperScript II reverse transcriptase II (Thermo Fisher Scientific, 18064014) and quantified by qPCR ...
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bioRxiv - Genetics 2021Quote: ... and then the quantification was conducted using the Quantifiler Human DNA Quantification Kit (Thermo Fisher Scientific) on an Applied Biosystems 7500 Real-time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: Human genomic DNA was extracted using the PureLink Genomic DNA Mini Kit (Thermo Fisher Scientific, USA) or QIAamp DNA Blood Mini Kit (QIAGEN ...
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bioRxiv - Microbiology 2020Quote: ... supernatants were collected and cytokine levels assed with Human IFNγ ELISA Kit (ThermoFisher Scientific, Vienna, Austria) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The subclass of each IgG antibody was determined using a human IgG subclass ELISA kit (Invitrogen).
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bioRxiv - Bioengineering 2022Quote: ... Anti-human IL6 Ab concentrations were quantified using the Quant-it protein assay kit (Life Technologies). Ab conjugates were validated on 10% TBE urea denaturing gels and validated on an agarose gel (Supplementary Figure S8) ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were isolated using a commercial kit (MagniSort Human CD4+ T Cell Enrichment; Affymetrix) and infected by incubation for 4 h at 37°C with 156,250 or 2,500 TCID50 (50% tissue culture infectious dose ...
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bioRxiv - Bioengineering 2022Quote: ... CD8+ T cells were positively enriched by Magnisort Human CD8+T cell positive selection Kit (Invitrogen). Nuclear fraction was isolated using NE-PER Nuclear and Cytoplasmic extraction kit (ThermoFisher) ...
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bioRxiv - Bioengineering 2019Quote: ... RNA from human cells was cleaned and collected utilizing an RNAqueous-Micro Kit (Ambion, cat. #AM1931) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... the human IL-2 ELISA kit was used in accordance with manufacturer’s instructions (Thermo Fisher Scientific). Murine IL-2 ELISA’s were performed using the mouse IL-2 ELISA kit (Thermo Fisher Scientific).
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bioRxiv - Immunology 2021Quote: Allogenic CD8+ were isolated by negative selection using Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen) and labeled with carboxyfluorescein succinimidyl ester (CFSE ...
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bioRxiv - Neuroscience 2023Quote: ELISA was performed on total and SEC fractions using Tau (Total) Human ELISA Kit (KHB0041, Invitrogen) by following the directions provided by the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was collected on day 3 and analyzed by human IL-2 ELISA Kit (ThermoFisher) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... and VIC labelled human β actin endogenous control probe (Human - 4326315E) or RNA28S5 (Human - Hs03654441_s1) (Thermo Fisher Scientific) so that amplified mRNA can be normalised to β actin or RNA28S5 ...
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bioRxiv - Neuroscience 2022Quote: ... and subsequently digested for 25 min at 37°C in a table shaker at 850 rpm in collagenase solution containing 1 mg/ml collagenase (type II, Gibco®, Grand Island, NY, USA) and 1 mg/ml pronase (Roche ...
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bioRxiv - Immunology 2021Quote: ... 0.03μL SuperScript II taq (Invitrogen), 0.03 DTT 0.1M (Invitrogen) ...
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bioRxiv - Plant Biology 2022Quote: ... via BP clonase II (Invitrogen) reaction following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Superscript II reverse transcriptase (Invitrogen) were used to reverse-transcribe mRNAs to cDNAs ...
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bioRxiv - Genetics 2019Quote: ... using LR Clonase II (Invitrogen). The resulting expression clone consisted of ...
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bioRxiv - Cell Biology 2021Quote: ... Phusion Hotstart II polymerase (Thermofisher) was used to generate all the inserts ...
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bioRxiv - Physiology 2021Quote: ... Countess II (Thermo Fisher Scientific). Cells were fed every other day with DMEM ...
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bioRxiv - Systems Biology 2021Quote: ... LR Clonase II (Thermofisher #11791020) and the lentiviral expression vector pLenti CMV Hygro DEST (Addgene #17454 ...
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bioRxiv - Microbiology 2020Quote: HotStart II polymerase (Thermo Scientific), and 16.5 μl molecular-grade water ...