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Citations for Thermo Fisher :
9101 - 9150 of 10000+ citations for Recombinant Rabbit CXCL12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: For analysis of the soluble protein fraction 50 µg protein were reduced with 2.5 mM TCEP (tris-(2-carboxyethyl) phosphine hydrochloride) (Invitrogen, Thermo Fisher Scientific) at 65 °C for 45 min before thiols were alkylated in 5 mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... A loading control was performed by total protein staining using Pierce Reversible Protein Stain Kit for Nitrocellulose Membranes (Thermo Fisher Scientific, 24580). For blocking ...
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bioRxiv - Cell Biology 2022Quote: ... Mitochondria enriched fractions were processed similar to whole cell samples except they were normalized via protein concentration as determined by the Pierce™ BCA Protein Assay kit (Thermo Scientific). In Brief ...
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bioRxiv - Cancer Biology 2022Quote: ... Li-COR chameleon duo pre-stained protein ladder (Li-COR, 928-60000) along with 100 μg of protein combined with loading buffer (Thermo Scientific, NP0007) were loaded onto a 4%-12% Bis-Tris gel (Thermo Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... The total protein levels in the samples were assayed using the Pierce™ BCA Protein Assay kit (Thermo Fisher Scientific; Cat. # 23227) and diluted with 500 mM TEAB so that the total protein level of each sample would be approximately the same ...
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bioRxiv - Developmental Biology 2022Quote: ... the protein concentration in the supernatant was measured using the Pierce BCA Protein Assay Kit (23225, Thermo Fisher Scientific, Waltham, MA, USA). After diluting with loading buffer and boiling for 10 min at 95°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Insoluble material was discarded and soluble proteins were recovered and quantified by the Bio-Rad Protein Assay (Hercules, CA) and the Nanodrop Drop One Spectrophotometer (Thermo Fisher Scientific). Cytoplasmic and nuclear extracts were obtained using the NE-PER Nuclear and Cytoplasmic Extraction reagent kit (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... 4 or 5 ug of appropriate antibodies or IgG control proteins were bound to protein G Dynabeads™ as per manufacturer instructions (Life Technologies). Equal portions of cleared lysates were added to antibody or IgG bound Dynabeads™ and incubated for 3 hours or overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... The total protein concentration for each culture was measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). Using the same amount of protein from each sample ...
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bioRxiv - Immunology 2022Quote: ... His-tagged proteins were purified from yeast supernatants by Ni-NTA resin affinity chromatography and protein concentration was determined using Coomassie (Bradford) Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Genomics 2022Quote: ... 10 min at 4°C and supernatants quantified for protein content using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23227) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2022Quote: ... Expression levels of proteins of interest were normalised by total protein amount (Ghosh et al, 2014) (Moritz, 2017) using a Pierce reversible protein stain kit (#24580, Thermo Fisher Scientific). Protein carbonylation was assessed with an Oxyblot protein oxidation detection kit (#S7150 ...
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bioRxiv - Physiology 2022Quote: GNM muscles of mice euthanized in the morning in fed state were ground in liquid nitrogen and proteins were extracted from the powder obtained using Mammalian protein extraction reagent (MPER, #78501, Thermo Fisher Scientific) supplemented with halt protease and halt phosphatase inhibitors (#78429 and #78426 ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations were estimated using the PierceTM BCA Protein Assay Kit (Cat# 23225, Pierce Biotechnology, Thermo Fisher Scientific Inc., Waltham, MA, USA). Proteins ...
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bioRxiv - Microbiology 2022Quote: ... Unlysed cells were removed by centrifugation at 15 700 rcf for 30 minutes at 4oC and the concentration of proteins within the clarified lysate was then measured using the Qubit Protein Assay (Thermofisher Scientific, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and a 1:10 dilution of 2 μl of the samples was used to measure protein concentration by Qubit Protein Assay (Invitrogen, Cat# Q33211). The remaining 10 μl were reduced ...
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bioRxiv - Plant Biology 2022Quote: ... the induction media was removed by centrifugation and proteins were extracted by the resuspension of bacteria in B-PER™ Bacterial Protein Extraction Reagent (ThermoFisher Scientific) with 0.8 U/mL DNase I (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... The homogenates were centrifuged at 4 °C for 30 min at 9300 × g and total protein concentration in the supernatants was determined using the Pierce™ BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Physiology 2023Quote: ... Total IL-1β protein level was measured by mouse IL-1β/IL-1F2 DuoSet ELISA kit and first normalized to total protein level quantified by Pierce™ BCA Protein Assay Kit (Thermo Scientific), and then normalized to the sham group.
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bioRxiv - Neuroscience 2022Quote: ... Peptide sequences (and hence protein identity) were determined by matching protein databases with the acquired fragmentation pattern by the SEQUEST software program (Thermo Fisher Scientific). The data were filtered to establish a false discovery rate between one and two percent by using a database of mouse protein sequences made up of half real protein sequences and half reversed sequences ...
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bioRxiv - Neuroscience 2022Quote: The proteomics was performed by the serum after removing common high-abundance protein through using a Thermo Fisher’s serum High Abundance protein removal reag ent (High Select™ Top14 Abundant Protein Depletion Mini Spin Columns, A36370, Thermo fisher, US). The mass spectrometer Thermo Scientific Q Exactive was perfor med for Label-free quantification detection and data was analyzed by Proteome Disco verer 2.2 ...
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bioRxiv - Molecular Biology 2022Quote: ... SDS at 100 volts for 70 min until the 14 kD protein marker in SeeBlue Plus2 pre-stained protein standard (Life Technologies, USA) reached the bottom end of the gel ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were cleared by centrifugation at 4°C and 23,000×g for 30 min and protein concentration determined using Pierce(tm) BCA Protein Assay (Thermo Fisher Scientific, USA). 0.2 μg/μl α-casein were added to a protein amount of 1.2 mg ...
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bioRxiv - Cell Biology 2022Quote: ... pDEST174HATMBP (for HAT [KDHLIHNVHKEEHAHNK]-MBP fusion proteins),52 or pDEST172HGST (for His6-GST fusion proteins) using Gateway LR Clonase II Enzyme mix (#11791020, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2022Quote: ... Plates were read using an MSD SECTOR Imager 2400 and resulting peptide concentrations were normalized to total protein in the cell lysate per well measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Data for each shRNA knockdown were additionally normalized to the average of control conditions for each parameter measured.
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... and cytosolic and membrane proteins were extracted with the use of a Mem-PER Plus Membrane Protein Extraction Kit (Thermo Fisher, 89842).
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bioRxiv - Biochemistry 2024Quote: ... and subcellular protein fractionation was performed on 1.0 x 107 cells using the Subcellular Protein Fractionation Kit for Cultured Cells (ThermoFisher Scientific, Waltham, MA). Protein concentrations were determined using bicinchoninic acid (BCA ...
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bioRxiv - Cell Biology 2024Quote: ... Following treatment cells were then transduced with Premo™ Autophagy tandem sensor Red Fluorescent Protein-Green Fluorescent Protein (RFP-GFP)-LC3B (Life Technologies, Invitrogen) and chloroquine was used as control ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration of each lysate was measured using the Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher Scientific, CA, USA). The 2′-3′-cGAMP content were measured by 2′-3′-cGAMP ELISA kit ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration of each sample was determined by the Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher Scientific, CA, USA). Equal amounts of proteins (up to 20 μg ...
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bioRxiv - Genomics 2024Quote: rox2RNA probes at 100 nM and hrp38 RNA probes at 50nM were incubated with MBP-tagged FL CLAMPWT protein or MBP-tagged FL CLAMPdelPrLD protein in REMSA binding buffer provided with the LightShift Chemiluminescent RNA EMSA kit (Thermo Scientific, USA) at room temperature for 30 min according to manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The pellet was resuspended in PBS/EDTA buffer and the protein concentration of the homogenate measured using the Pierce BCA protein assay kit (Thermo Scientific, USA). AChE activity was measured using the Ellman assay using acetylthiocholine as substrate and the indicator 5,5′-dithiobis-2-nitrobenzoic acid (DTNB ...
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bioRxiv - Molecular Biology 2023Quote: ... a mass calibration was recorded to allow conversion between contrast to mass using a protein standard (Invitrogen NativeMark™ Unstained Protein Standard) with masses of 1236 ...
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bioRxiv - Developmental Biology 2024Quote: Protein synthesis was assessed using the Click-iT® L-homopropargylglycine (HPG) Alexa Fluor 488 Protein Synthesis Assay Kit (Molecular Probes, USA), which measures newly synthesized proteins through quantification of the fluorescent HPG signal ...
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bioRxiv - Immunology 2024Quote: ... To measure the protein content of the lysate a Qubit 4 fluorometer was used using the Qubit protein assay kit (Thermo Fisher Scientific). Equal amounts of pre-cleared lysate samples were separated in 8 – 16% Mini protean-TGX stain free protein gel (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... Final protein concentration of the pooled elution fraction was determined with the Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA) before being used for in vitro acetylation.
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bioRxiv - Microbiology 2024Quote: ... The concentration of the purified proteins was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, United States) as described previously (Choi et al. ...
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bioRxiv - Cell Biology 2023Quote: ... was added as a supplement to the lysis buffer and the protein concentration was measured using a Pierce BCA Protein Assay Kit (#23225, Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... protein concentrations were measured with the Pierce BCA protein assay kit coupled with the bovine serum albumin standards (23225, Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: Cells were lysed in 1x RIPA Buffer pre-warmed to 95°C and the total protein concentration was normalised using the Pierce BCA Protein Assay (Cat. # 23227; Thermo Fisher Scientific). Proteins (20 µg ...
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bioRxiv - Cell Biology 2023Quote: ... Volumes of lysate containing equal amounts of total protein were used to immunoprecipitate RACK1 by incubating with mouse monoclonal antibody against HA and protein G agarose beads (Thermo Fisher, 15920010) at 4°C with gentle mixing ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected after centrifugation (12,000 x g, 20 min) and total protein levels were measured using Pierce™ BCA Protein Assay Kits (Thermo Scientific #23227). Proteins were denatured by boiling with Laemmli buffer (Bio-Rad #1610747 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Protein content of all samples analyzed by SDS-PAGE was estimated using a Pierce bicinchoninic acid (BCA) protein assay (Thermo Scientific, Germany) using the 96-well plate protocol as recommended by the manufacturer.
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bioRxiv - Microbiology 2023Quote: ... calcitrans salivary proteins by size with proteins visualised by Coomassie brilliant blue dye or silver staining (Pierce™ Silver Stain Kit, ThermoFisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... Whole cell lysates were centrifuged for 15 min at maximum speed at 4°C and protein concentrations measured from the supernatant with a Pierce BCA Protein Assay Kit (Thermo Scientific, 23227). 15 µg protein per lane were resolved on 8% SDS-PAGE ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 °C for 20 min and supernatants were transferred into french tubes before protein quantification using the Pierce BCA protein assay (Thermo Fisher Scientific). Lysate equivalent to 10-50 µg of proteins was mixed to 1× Laemmli buffer (50 mM Tris ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected after centrifugation for 10 min at 15 x 1000 g at room temperature and were used for protein quantification with BCA protein assay (Thermo Fisher, 23225).
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bioRxiv - Neuroscience 2023Quote: ... the protein concentrations of synaptosome samples resuspended in HEPES buffer were tested by the Pierce BCA Protein Assay Kit (Thermo Fisher #23225) following the manufacturer’s instructions and then diluted to 1 µg/µL ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were removed and the protein concentration was quantified using the colorimetric assay by PierceTM BCA protein assay kit (ThermoFisher Scientific, 23225). Protein lysates were resolved using 10% SDS-PAGE gels and transferred to 0.45 µm PVDF membranes (Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifuging at 12.000 rpm for 5 min at 4°C and protein concentration determined using the Pierce™ BCA Protein Assay (Thermo Scientific 23225). Samples were prepared for SDS-Page in 1× NuPAGE™ LDS Sample Buffer containing 50mM DTT ...