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Citations for Thermo Fisher :
8901 - 8950 of 10000+ citations for Recombinant Rabbit CXCL12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The collected supernatant was used to measure protein concentration using the BCA assay (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was used to determine the protein concentration by BCA assay according to the manufacturer’s instructions (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific).
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bioRxiv - Genetics 2023Quote: Cultured human iPSC-derived NGN2 neurons were maintained in 6-well plates and were lysed on day 21-24 for membrane protein extraction using the Mem-PER Plus Membrane Protein Extraction kit (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... Protein concentrations in the supernatant were determined using the Pierce Micro BCA Protein Assay kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: ... Protein levels of the brain tissue were measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Cell Biology 2023Quote: ... Debris was removed by centrifugation (4°C, 30 min, 20’000 g) and total protein concentrations measured using the Bradford protein assay (Thermo Fisher Scientific). Typically ...
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bioRxiv - Plant Biology 2023Quote: ... The homogenate was centrifuged and the protein concentration of the supernatant was measured using a Pierce 660 nm Protein Assay Reagent (Thermo Scientific). Thirty-five microgram of protein was loaded in each lane ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was collected after centrifuging at 13,000 RPM (10 mins, 4°C) and total protein was estimated by BCA (Pierce BCA Protein Assay Kit, ThermoFisher Scientific, 23225). The supernatant was mixed with 1X Laemmli buffer and boiled at 95°C for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... The same amount of protein was used for the enrichment of biotinylated proteins using100 μl Pierce™ streptavidin agarose beads (Cat. # 20353, ThermoFisher) overnight at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... Supernatants were collected and protein concentration was determined using a Pierce™ BCA Protein Assay Kit (Cat#: 23225, Thermo Fisher scientific) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Total proteins from intestinal epithelial cells or intestinal organoids were extracted with M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific) with proteinase inhibitor (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... For normalization total protein concentration was measured on an aliquot of the cell lysate with the Pierce BCA protein kit (Thermo Fisher) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 μg of antibodies against Piwi and Aubergine were each coupled to 50:50 of protein-A and protein-G Dynabeads (Life Technologies). Lysates were incubated with bead-coupled antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... Antibodies were captured by 20 µ l of a 50:50 mix of Protein-A and Protein-G Dynabeads (Life Technologies). The beads were washed several times sequentially in the buffers containing low salt (150 mM NaCl) ...
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bioRxiv - Cell Biology 2023Quote: ... samples were collected by centrifugation (11,000 ×g, 20 min) and their protein content was determined (Thermo Pierce BCA Protein Assay kit, ThermoFisher, Cat# 23225). Proteins (∼30 µg/lane ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein lysate from each sample was collected and the concentration of the protein was estimated using Pierce™ BCA Protein Assay Kit (ThermoFisher). For each sample ...
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bioRxiv - Immunology 2023Quote: ... Bound immune complexes were captured with an equal mix of protein A and protein G Dynabead magnetic beads (Thermo Fisher Scientific), which recognize all mouse IgG subclasses ...
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bioRxiv - Genetics 2023Quote: ... The concentration of the liberated soluble protein was assessed with the Pierce BCA protein assay kit (Thermo Fisher, Waltham, MA, USA), and equal amounts of protein (180-250 µg ...
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bioRxiv - Immunology 2023Quote: ... Protein concentration of the supernatant was determined by following manufacturers instruction for the Pierce BCA Protein Assay Kit (ThermoFisher-Scientific #23227). Once total protein concentration was determined ...
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bioRxiv - Biochemistry 2023Quote: Purity of all proteins was verified by SDS-PAGE and concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Biochemistry 2023Quote: Purity of all proteins was verified by SDS-PAGE and concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Molecular Biology 2023Quote: The accumulation of DNA damage response proteins to chromatin following IR-induced DNA damage was examined using the Subcellular Protein Fractionation Kit (Thermo Fisher) as previously described (23) ...
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bioRxiv - Molecular Biology 2024Quote: ... Total protein concentration in the supernatant was determined using the Micro BCA protein assay kit (Thermo Scientific, Waltham, MA; Cat.: 23235). In the same supernatant ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were lysed at 4°C with RIPA buffer and protein concentration was determined with the Micro kit BCA Protein Assay (Thermo Scientific, Courtaboeuf ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentration was measured to be 0.1 mg/ml by the BCA protein assay kit (Thermo Fisher Scientific Inc., Waltham, MA). To make cryo-specimen of Fab fragment ...
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bioRxiv - Molecular Biology 2023Quote: ... The cell lysate was then sonicated and centrifuged at 16,000g for 15min and the supernatants were extracted for protein concentration measurement by Pierce BCA protein assay (ThermoFisher, Cat# 23227). For the samples of differentiation time course ...
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bioRxiv - Bioengineering 2023Quote: Protein contents of the SEC fractions were measured using a BCA protein assay kit (23225, Pierce, Thermo Fisher Scientific, MA, USA). A standard curve (range 0–1000 μg/ml ...
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Volumetric Compression Shifts Rho GTPase Balance and Induces Mechanobiological Cell State TransitionbioRxiv - Biophysics 2023Quote: ... A small aliquot of cell lysate was later thawed and used for total protein quantification following the “BCA Protein Assay Kit” manual (Thermo Scientific). The same quantity (60 μL ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were harvested and lysed prior to splitting each sample into two aliquots for D-2-HG quantification and total protein quantification using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... Chromatin was immunoprecipitated by adding 10µl of both protein A and protein G-conjugated paramagnetic beads (Invitrogen #1001D and #1003D, respectively) and incubated at 4°C for 1h ...
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bioRxiv - Genomics 2023Quote: ... Then the supernatant was collected and the protein concentration was measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Rabbit polyclonal anti-HMGB2 (1:1,000 ...
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bioRxiv - Neuroscience 2023Quote: ... We obtained 40-80 μg of histone proteins per extraction and loaded 20 μg of protein onto a 4-12% Bis-Tris Plus mini gel (Invitrogen# NW04120BOX). Gels were run for ∼20 minutes at 200V using the Invitrogen mini gel tank with Blot MES SDS Running buffer ...
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bioRxiv - Microbiology 2023Quote: ... and the supernatants were subsequently clarified with 50 µL of magnetic beads coupled to protein G (Dynabeads Protein G-LS10003D, Thermo Scientific) at 4°C for 1 h with agitation ...
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bioRxiv - Microbiology 2023Quote: ... on ice for 30 min and the protein concentration was determined using the Pierce™ BCA protein assay kits (Thermo Fisher) to ensure equal loading ...
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bioRxiv - Cancer Biology 2023Quote: ... 5-20 μg of total protein was separated by NuPAGE 4-12% Bis-Tris Mini Protein Gels (NP0336BOX; Thermo Fisher Scientific) and transferred to Immobilon-P PVDF Membrane (IPVH00010 ...
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bioRxiv - Biochemistry 2023Quote: ... The protein in the final sample was quantified using the Qubit Protein Assay Kit with the Qubit 2.0 Fluorometer (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations of the lysed sEV preparations were quantified using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, USA), following the manufacturer’s protocols (Leung et al. ...
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bioRxiv - Immunology 2023Quote: ... Equivalent amounts of total protein lysate were subjected to electrophoresis on NuPAG 4–12% Bis-Tris Protein Gels (Thermo Fisher Scientific) and proteins were transferred to polyvinylidene difluoride membranes (Millipore) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Protein concentrations of the soluble cellular protein and 100x concentrated secreted protein samples were quantified against Bovine Serum Albumin (BSA) standards using the Pierce Coomassie Protein Assay kit (#23236, ThermoFisher Scientific). The assays were performed in 96-well transparent plates containing 150 μl of diluted protein sample or 0 ...
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bioRxiv - Synthetic Biology 2024Quote: ... We purchased B-PER™ Complete Bacterial Protein Extraction Reagent and Pierce™ 660 nm Protein Assay Reagent from Thermo Fisher Scientific (USA) ...
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bioRxiv - Cell Biology 2024Quote: ... The obtained protein lysate was subsequently used for Co-IP of the Prm2-Septin12 protein complex by Dynabeads Protein G Immunoprecipitation kit (10007D, Invitrogen, USA). Magnetic beads were conjugated with anti-protamine 2 antibody (Mab-Hup2B ...
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bioRxiv - Genetics 2024Quote: ... protein levels were normalized by total protein amount in the same lane as determined by Novex Reversible Membrane Protein Stain (Thermo Scientific) performed prior to blocking.
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bioRxiv - Cell Biology 2024Quote: ... The instrument was calibrated by addition of 15 µl of protein standards from Native Mark Unstained Protein Standard (Thermo Fisher LC0725) in buffer A (10 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples of the resuspended protein and the streptavidin bead-bound protein were prepared in NuPAGE LDS Sample Buffer (Thermo Fisher Scientific) supplemented with ꞵ-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Sample protein content was determined with the Pierce detection assay and 200-800 µg of proteins were loaded on Dynabeads Protein G for Immunoprecipitation (ThermoFisher Scientific); the beads were previously incubated for 10 min on a wheel at room temperature with PBS-Tween-20 0.01% supplemented or not with 2.5-5 µg of MIC60 ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was transferred to a new tube before determining protein concentration by means of BCA protein assay (Pierce, Thermo Fisher). Protein aliquots were stored at −30 °C until use.
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bioRxiv - Molecular Biology 2024Quote: ... The tissue lysate was centrifuged at 16,000 x g for 20 min and supernatant was extracted for protein concentration measurement by Pierce BCA protein assay (ThermoFisher, # 23227). Each amount of protein was loaded into 8-16% Mini-PROTEIN TGX Precast Protein Gels (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... The EPR signal intensities were normalized to the protein concentrations of the samples as determined by BCA protein assay kit (Thermofisher ScientificTM).
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bioRxiv - Cell Biology 2024Quote: ... Lysates were spun down at 10,000 RPM and supernatant collected for protein quantification using a Pierce™ BCA Protein Assay Kit (ThermoFisher-23225). NuPAGE™ 4% to 12% ...
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bioRxiv - Neuroscience 2024Quote: ... before membranes were dried overnight 2 h at room temperature and proteins stained with Sypro Ruby Protein Blot Stain (ThermoFisher Scientific), according to the manufacturer’s instructions ...