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Citations for Thermo Fisher :
9051 - 9100 of 10000+ citations for Recombinant Rabbit CXCL12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... and the contrast value of each protein molecule was converted to mass with calibration obtained from an unstained protein ladder (LC0725, Thermofisher, Wattham, MA).
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration of each lysate was measured using the BCA protein assay kit according to manufacturer’s directions (Thermo-Fisher Scientific, Waltham, MA). Proteins were separated on a 10% SDS-PAGE gel with equivalent amounts of protein loaded in each lane (Thermo-Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... 10-20 μg of purified proteins were separated by SDS-PAGE and stained with mass spectrometry compatible Imperial protein stain (Thermo Fisher Scientific). For the identification of phosphorylation site(s ...
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bioRxiv - Microbiology 2022Quote: ... China).The purity of the protein was assessed by SDS-PAGE gel and its concentration was determined using the BCA Protein Assay Reagent (Thermo Fisher Scientific). AdpA cysteine mutant proteins (C62S ...
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bioRxiv - Genetics 2022Quote: Total protein was isolated from kidneys or cells using lysis buffer made from mixing T-PER tissue protein extraction reagent (Invitrogen, catalog# 78510) with a protease phosphatase inhibitor tablet (Fisher ...
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bioRxiv - Microbiology 2022Quote: ... Cleavage of the spike protein was detected using anti-SARS-CoV Spike Protein (SDelta3) monoclonal antibody (7G12, Thermo Fisher Scientific, CA, USA)) raised against immunogen aa168-461 of the S1 domain.
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bioRxiv - Biochemistry 2022Quote: ... Protein concentrations were determined using the Thermo Scientific™ Pierce™ BCA (bicinchoninic acid) Protein Assay Kit (Thermo Scientific™ Cat# 23225). Following concentration determination ...
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bioRxiv - Neuroscience 2021Quote: ... Antibody-bound NLGNs were incubated for 1 hour with 20 µL of protein G beads (Dyna-beads Protein G, Thermo Fisher Scientific) precipitated and washed 4 times with lysis buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Lysates were passed through a 0.22 μm Spin-X filter column after which total protein amount was determine using the Pierce BCA Protein Assay Kit (#23225, Thermo Fisher Scientific). Equal protein amounts were denatured in SB and subjected to SDS-PAGE ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations in the tissue lysates were measured with the aid of a bicinchoninic acid protein assay kit (Thermo Scientific, Rockford, IL). TNF-α ...
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bioRxiv - Microbiology 2020Quote: ... The supernatants were transferred to new ice-cold Eppendorf tubes and protein concentration was measured by the BCA assay using Pierce BCA Protein Assay kit (ThermoFisher Scientific; #23225). Equal amounts of protein were loaded on 4-12% SDS-PAGE gel and transferred onto nitrocellulose membrane ...
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bioRxiv - Microbiology 2021Quote: ... microsphere mixtures were successively incubated with serum samples (1:400) biotinylated protein A and biotinylated protein G (4 μg/ml each) (Thermo Fisher Scientific), and Streptavidin-R-Phycoerythrin (4 μg/ml ...
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bioRxiv - Bioengineering 2021Quote: ... Total protein quantification was performed in samples using Pierce™ BCA (Bicinchoninic Acid) Protein Assay Kit (Thermo Fisher Scientific Inc. Waltham, MA) following manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: ... The purity and quality of purified protein samples were checked using SDS-PAGE and concentration was measured using bicinchoninic acid (BCA) protein assay (Thermo Scientific, USA).
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bioRxiv - Immunology 2020Quote: ... sonicated 3 three times for 10 sec each and analysed for protein content using the BCA protein assay kit (Thermo Fisher scientific). Samples containing 30 µg/µl of cell lysate proteins per lane were resolved on SDS-PAGE (10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis ...
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bioRxiv - Neuroscience 2021Quote: ... the cleared supernatant was collected and the protein content in the soluble fraction was determined using the Pierce® BCA Protein Assay Kit (Thermo Scientific) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were denatured by boiling at 95°C for 10 mins and subjected to a PierceTM BCA protein assay (Life Technologies, 23227) for determination of the protein concentration ...
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bioRxiv - Immunology 2022Quote: ... The final Spike protein concentration was determined by measuring the absorbance at 562 nm using Pierce™ BCA Protein Assay Kit (Thermo Scientific). Final proteins were dispensed in aliquots of 0.5 ml each and stored at -80°C prior to use.
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bioRxiv - Cancer Biology 2022Quote: ... The total protein lysates and the membrane and cytoplasmic protein fractions were mixed with NuPage™ LDS sample buffer (Thermo Fisher Scientific) and analyzed by Western blotting ...
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bioRxiv - Cancer Biology 2022Quote: ... The same protein samples were loaded onto a 15% SDS polyacrylamide gel and then stained with SYPRO Ruby Protein Gel Stain (Thermo Fisher Scientific) to confirm an equivalent sample loading in each lane.
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bioRxiv - Cell Biology 2022Quote: Each extracellular protein fraction (#1-15) collected by UF-SEC was collected and protein concentration determined by Micro BCA assay (Thermo Fisher Scientific) as according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Fractions of pure proteins from the gels were pooled together and the protein stock concentrations were measured using the BCA assay (Thermo Fisher Scientific).
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bioRxiv - Systems Biology 2019Quote: ... Protein pellets were dissolved in 200 uL 5% SDS solution and protein concentrations were determined using Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific).
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bioRxiv - Physiology 2019Quote: ... Cells were scraped in PBS and a small aliquot was used to measure total protein using a Coomassie protein assay (Thermo Scientific, #1856209). Cells were lysed in cell extract buffer (100 mM Tris ...
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bioRxiv - Microbiology 2019Quote: ... The protein purity was assessed on SDS-PAGE with colloidal coomassie staining45 and protein yield was quantified by BCA-assay (ThermoFisher Scientific, USA). Imidazole and salts were removed by three washing steps with 21 ml dialysis buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Plant Biology 2019Quote: ... The protein concentration of the clear supernatant was determined using the bicinchoninic acid assay (BCA Protein Assay Kit by Thermo Scientific Pierce).
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bioRxiv - Cell Biology 2019Quote: ... Protein concentrations of the tissue homogenates or whole-cell extracts were determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... The 3 eluted fractions were pooled and the protein concentrations measured with the Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, 23225) using the Pierce™ Bovine Gamma Globulin Standard (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... CLICK-IT enrichment of AHA-labelled secreted proteins was performed with the Click-iT™ protein enrichment kit (Thermo Fisher Scientific, C10416) as previously described67,68 ...
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bioRxiv - Microbiology 2020Quote: ... Pre-cleared lysates were further incubated at 4°C overnight with the indicated antibodies (1 to 3 μl) and protein A agarose or protein G Dynabeads (Thermo Fisher Scientific). Immunoprecipitates were washed three times with RIPA buffer (LSB ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The protein content of supernatants was measured using a BiCinchoninic acid Assay (BCA) protein assay kit (Thremo Fisher Scientific, Mississauga, ON, Canada). Equal amounts of protein in lysis buffer (10 µg ...
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bioRxiv - Neuroscience 2021Quote: ... An input sample of 75 μl was removed from the lysate and protein concentration was assessed using a Pierce Bicinchoninic acid (BCA) protein assay kit (Thermo Scientific, 10678484). Concomitantly ...
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bioRxiv - Developmental Biology 2019Quote: ... Total protein was extracted from the homogenized whole kidney lysates using the M-PER mammalian protein extraction reagent (Thermo Fisher Scientific, 78501) supplemented with protease (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... 3.75% w/v stacking gel) prepared as described elsewhere [72] and run alongside a protein standard (NativeMark Unstained Protein Standard, Thermo Fisher Scientific) at 25 mA for 3 hr ...
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bioRxiv - Molecular Biology 2019Quote: ... The supernatant was placed in a new tube and the protein concentration was determined using Pierce BCA Protein Assay (Thermo Scientific 23227). Each reaction contained 15μg protein in a final volume of 230μl Proteasome buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were diluted to 2% (w/v) SDS and protein concentrations were determined using the Pierce BCA Protein Assay (Thermo Scientific, #23225). 20 μg of each lysate were subjected to an in-solution tryptic digest using a modified version of the Single-Pot Solid-Phase-enhanced Sample Preparation (SP3 ...
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bioRxiv - Physiology 2020Quote: ... followed by a 4-h incubation in Protein A magnetic beads (Pierce™ Protein A Magnetic Beads, #88845, ThermoFisher Scientific, Waltham, USA) and isolated using a magnetic rack ...
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bioRxiv - Physiology 2021Quote: ... The total concentration of proteins was measured with the Pierce Rapid Gold BCA Protein assay kit (Thermo Fisher Scientific, Rockford, IL, USA). The E- ...
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bioRxiv - Cell Biology 2021Quote: ... and an equal protein mass of 30ug in 10μL was subjected to NuPAGE™ 4-12 % Bis-Tris Midi Protein Gels (Invitrogen™, NP0335BOX) in Xcell4 Surelock tank (Life Technology SAS ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mM MgCl2) for cell membrane binding proteins (LPL, Caveolin 1) and quantified with Pierce™ BCA Protein Assay Kit (Buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were lysed in ddH2O by freeze/thaw cycles and the protein concentration was determined using the Pierce™ BCA protein assay kit (ThermoFisher Scientific). Polyamines were extracted by Bligh and Dyer extraction (Noga et al. ...
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bioRxiv - Plant Biology 2020Quote: ... Samples were resuspended in 2x Laemmli buffer and quantified for protein concentration using the Pierce 660nm Protein Assay Reagent (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2020Quote: A total of 85 μg of protein per sample was subjected to one-dimensional SDS-PAGE using an 8-16% precise protein gradient gel (Thermo Fisher Scientific). Proteins were separated for 1 cm in the resolving gel and the entire lane was excised and cut into equal pieces ...
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bioRxiv - Immunology 2020Quote: ... or with two administrations two weeks apart of 2.5μg Spike protein (2019-nCoV Spike Protein-S1+S2 ECD, His tag, SinoBiological) formulated in final 20mg/ml Imject™ Alum Adjuvant (Thermo Scientific) via bilateral intramuscular injections in the quadriceps (50μl per side) ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were clarified by centrifugation (12,000 g for 30 min at 4 °C) and protein concentrations were determined using a BCA protein assay kit (Thermo Fisher Scientific). SOD activity in whole cell extracts was measured using the EnzyChrome superoxide dismutase assay kit (BioAssay Systems ...
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bioRxiv - Neuroscience 2020Quote: ... ATP quantities were finally referred to protein amount using the Bradford assay (Coomassie Plus™ Protein Assay Reagent, Thermo Scientific, Schwerte, Germany).
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bioRxiv - Cell Biology 2020Quote: ... Cell debris were removed by centrifugation at 1000 × g for 5 min at 4°C and the protein concentration of the resulting supernatant was estimated using the BCA protein assay kit (Thermo Fisher Scientific) with bovine serum albumin as the standard ...
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bioRxiv - Cell Biology 2021Quote: ... Guanidine lysis buffer (above) was added to frozen pellets for sonication and protein concentration was assessed using BCA Protein Assay Reagent (Thermo Scientific, v23225). Lysates were equalized for protein concentration and incubated with NiNTA agarose beads (Qiagen ...
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bioRxiv - Genetics 2020Quote: ... incubated for 5 min at 95 °C and 80 μg of protein was separated on a 10% SDS-PAGE gel (alongside PageRuller™ Plus Prestained protein Ladder, Thermo Scientific). Samples were transferred onto a nitrocellulose membrane (Amersham Protan 0.45 μm ...
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bioRxiv - Molecular Biology 2021Quote: ... the membranes were cut in around the 55 kDa marker band of the protein marker (PageRuler Prestained Protein Ladder, Thermo Fisher Scientific). The upper portion of the membrane was probed with anti-HA tag antibody (mouse ...