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Citations for Thermo Fisher :
8951 - 9000 of 10000+ citations for West Nile Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: GNM muscles of mice euthanized in the morning in fed state were ground in liquid nitrogen and proteins were extracted from the powder obtained using Mammalian protein extraction reagent (MPER, #78501, Thermo Fisher Scientific) supplemented with halt protease and halt phosphatase inhibitors (#78429 and #78426 ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations were estimated using the PierceTM BCA Protein Assay Kit (Cat# 23225, Pierce Biotechnology, Thermo Fisher Scientific Inc., Waltham, MA, USA). Proteins ...
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bioRxiv - Microbiology 2022Quote: ... Unlysed cells were removed by centrifugation at 15 700 rcf for 30 minutes at 4oC and the concentration of proteins within the clarified lysate was then measured using the Qubit Protein Assay (Thermofisher Scientific, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and a 1:10 dilution of 2 μl of the samples was used to measure protein concentration by Qubit Protein Assay (Invitrogen, Cat# Q33211). The remaining 10 μl were reduced ...
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bioRxiv - Plant Biology 2022Quote: ... the induction media was removed by centrifugation and proteins were extracted by the resuspension of bacteria in B-PER™ Bacterial Protein Extraction Reagent (ThermoFisher Scientific) with 0.8 U/mL DNase I (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... The homogenates were centrifuged at 4 °C for 30 min at 9300 × g and total protein concentration in the supernatants was determined using the Pierce™ BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Physiology 2023Quote: ... Total IL-1β protein level was measured by mouse IL-1β/IL-1F2 DuoSet ELISA kit and first normalized to total protein level quantified by Pierce™ BCA Protein Assay Kit (Thermo Scientific), and then normalized to the sham group.
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bioRxiv - Neuroscience 2022Quote: ... Peptide sequences (and hence protein identity) were determined by matching protein databases with the acquired fragmentation pattern by the SEQUEST software program (Thermo Fisher Scientific). The data were filtered to establish a false discovery rate between one and two percent by using a database of mouse protein sequences made up of half real protein sequences and half reversed sequences ...
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bioRxiv - Neuroscience 2022Quote: The proteomics was performed by the serum after removing common high-abundance protein through using a Thermo Fisher’s serum High Abundance protein removal reag ent (High Select™ Top14 Abundant Protein Depletion Mini Spin Columns, A36370, Thermo fisher, US). The mass spectrometer Thermo Scientific Q Exactive was perfor med for Label-free quantification detection and data was analyzed by Proteome Disco verer 2.2 ...
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bioRxiv - Molecular Biology 2022Quote: ... SDS at 100 volts for 70 min until the 14 kD protein marker in SeeBlue Plus2 pre-stained protein standard (Life Technologies, USA) reached the bottom end of the gel ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were cleared by centrifugation at 4°C and 23,000×g for 30 min and protein concentration determined using Pierce(tm) BCA Protein Assay (Thermo Fisher Scientific, USA). 0.2 μg/μl α-casein were added to a protein amount of 1.2 mg ...
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bioRxiv - Cell Biology 2022Quote: ... pDEST174HATMBP (for HAT [KDHLIHNVHKEEHAHNK]-MBP fusion proteins),52 or pDEST172HGST (for His6-GST fusion proteins) using Gateway LR Clonase II Enzyme mix (#11791020, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2022Quote: ... Plates were read using an MSD SECTOR Imager 2400 and resulting peptide concentrations were normalized to total protein in the cell lysate per well measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Data for each shRNA knockdown were additionally normalized to the average of control conditions for each parameter measured.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The pellet was resuspended in PBS/EDTA buffer and the protein concentration of the homogenate measured using the Pierce BCA protein assay kit (Thermo Scientific, USA). AChE activity was measured using the Ellman assay using acetylthiocholine as substrate and the indicator 5,5′-dithiobis-2-nitrobenzoic acid (DTNB ...
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bioRxiv - Neuroscience 2023Quote: ... was recombinantly expressed as an N-terminal Fc-fusion protein (Fc-hTfR1) in Expi293 suspension cells and purified by gravity flow with PierceTM Protein A Agarose (Thermo Fisher Scientific). HFCII probes were loaded with Fc-hTfR1 by thresholding to a 1 nm shift ...
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bioRxiv - Plant Biology 2024Quote: ... protein concentrations of fractions containing FtsZ1-2 and FtsZ2-1 were determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: ... Protein content of the crude mitochondrial fraction was measured by BCA assay (Pierce BCA Protein Assay Kit, Thermo Scientific catalog number 23225). Thirty µg of mitochondria protein was incubated at 25 °C in 850 µL reaction buffer (100 mM Tris-HCl ...
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bioRxiv - Neuroscience 2024Quote: ... The protein concentrations in the cytoplasmic and nuclear fractions were determined using a Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Cat. 23228). The isolated cytoplasmic and nuclear proteins were aliquoted and stored at -80°C for subsequent analysis.
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bioRxiv - Genomics 2024Quote: rox2RNA probes at 100 nM and hrp38 RNA probes at 50nM were incubated with MBP-tagged FL CLAMPWT protein or MBP-tagged FL CLAMPdelPrLD protein in REMSA binding buffer provided with the LightShift Chemiluminescent RNA EMSA kit (Thermo Scientific, USA) at room temperature for 30 min according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... a mass calibration was recorded to allow conversion between contrast to mass using a protein standard (Invitrogen NativeMark™ Unstained Protein Standard) with masses of 1236 ...
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bioRxiv - Microbiology 2024Quote: ... The concentration of the purified proteins was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, United States) as described previously (Choi et al. ...
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bioRxiv - Microbiology 2024Quote: ... Final protein concentration of the pooled elution fraction was determined with the Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA) before being used for in vitro acetylation.
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bioRxiv - Cell Biology 2023Quote: ... was added as a supplement to the lysis buffer and the protein concentration was measured using a Pierce BCA Protein Assay Kit (#23225, Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2024Quote: Protein synthesis was assessed using the Click-iT® L-homopropargylglycine (HPG) Alexa Fluor 488 Protein Synthesis Assay Kit (Molecular Probes, USA), which measures newly synthesized proteins through quantification of the fluorescent HPG signal ...
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bioRxiv - Immunology 2024Quote: ... To measure the protein content of the lysate a Qubit 4 fluorometer was used using the Qubit protein assay kit (Thermo Fisher Scientific). Equal amounts of pre-cleared lysate samples were separated in 8 – 16% Mini protean-TGX stain free protein gel (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... and subcellular protein fractionation was performed on 1.0 x 107 cells using the Subcellular Protein Fractionation Kit for Cultured Cells (ThermoFisher Scientific, Waltham, MA). Protein concentrations were determined using bicinchoninic acid (BCA ...
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... and cytosolic and membrane proteins were extracted with the use of a Mem-PER Plus Membrane Protein Extraction Kit (Thermo Fisher, 89842).
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bioRxiv - Cell Biology 2024Quote: ... Following treatment cells were then transduced with Premo™ Autophagy tandem sensor Red Fluorescent Protein-Green Fluorescent Protein (RFP-GFP)-LC3B (Life Technologies, Invitrogen) and chloroquine was used as control ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration of each lysate was measured using the Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher Scientific, CA, USA). The 2′-3′-cGAMP content were measured by 2′-3′-cGAMP ELISA kit ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration of each sample was determined by the Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher Scientific, CA, USA). Equal amounts of proteins (up to 20 μg ...
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bioRxiv - Plant Biology 2023Quote: ... The lysate was cleared by centrifugation (18,000 x g for 10 min at 25°C) and the protein content of the supernatant was determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific). Reduction ...
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bioRxiv - Microbiology 2023Quote: ... calcitrans salivary proteins by size with proteins visualised by Coomassie brilliant blue dye or silver staining (Pierce™ Silver Stain Kit, ThermoFisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... Whole cell lysates were centrifuged for 15 min at maximum speed at 4°C and protein concentrations measured from the supernatant with a Pierce BCA Protein Assay Kit (Thermo Scientific, 23227). 15 µg protein per lane were resolved on 8% SDS-PAGE ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 °C for 20 min and supernatants were transferred into french tubes before protein quantification using the Pierce BCA protein assay (Thermo Fisher Scientific). Lysate equivalent to 10-50 µg of proteins was mixed to 1× Laemmli buffer (50 mM Tris ...
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bioRxiv - Synthetic Biology 2023Quote: ... Protein content of all samples analyzed by SDS-PAGE was estimated using a Pierce bicinchoninic acid (BCA) protein assay (Thermo Scientific, Germany) using the 96-well plate protocol as recommended by the manufacturer.
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bioRxiv - Physiology 2023Quote: ... BAL cells were counted using a LUNA-II automated cell counter (Logos Biosystems) and BAL supernatant total protein was measured using a Pierce total protein assay (Thermo Scientific, #23225).
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bioRxiv - Cancer Biology 2023Quote: ... Peptide sequences (and hence protein identity) were determined by matching protein databases with the acquired fragmentation pattern by Sequest (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were removed and the protein concentration was quantified using the colorimetric assay by PierceTM BCA protein assay kit (ThermoFisher Scientific, 23225). Protein lysates were resolved using 10% SDS-PAGE gels and transferred to 0.45 µm PVDF membranes (Millipore ...
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bioRxiv - Immunology 2023Quote: ... they are displayed as area ratio and were normalized to protein content measured using Pierce TM BCA protein assay kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: Samples (input (eluted in 2× SDS buffer and thawed 15 minutes at 75°C) and IP proteins) were run in NuPAGE 4%–12% Bis-Tris Mini Protein Gel (10 wells) (ThermoFisher Scientific, NP0321BOX) at the voltage of 100V for 2.5 hours in a NuPAGE MOPS SDS Running buffer (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... Twenty-five microliter of the slurry was centrifuged and the supernatant was subjected to protein concentration determination using Pierce TM BCA protein assay Kit (Thermo Fisher Scientific). The protein slurry was diluted to 1 ng/µl with 100 mM Tris pH 8 ...
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bioRxiv - Physiology 2023Quote: At day 45 (± 2 days) protein was extracted from beating iPSC-CMs in protein lysis buffer (1 X Pierce™ RIPA buffer (Thermo Fisher); 1 X PhosSTOP cocktail (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifuging at 12.000 rpm for 5 min at 4°C and protein concentration determined using the Pierce™ BCA Protein Assay (Thermo Scientific 23225). Samples were prepared for SDS-Page in 1× NuPAGE™ LDS Sample Buffer containing 50mM DTT ...
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bioRxiv - Bioengineering 2023Quote: ... The amount of affibody conjugated to the polymer was quantified by dissolving 2-3 mg of HA-Nor-Ox-Affibody in PBS at 2% w/v and determining the protein concentration of the solution using a Pierce 660 Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... The membrane pellet was resuspended in the same buffer and the protein concentration was determined spectroscopically with the BCA protein assay kit (Thermo Fisher Scientific). The membrane fraction was supplemented with n-dodecyl-β-D-maltoside (DDM ...
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bioRxiv - Physiology 2023Quote: ... Tau protein was also labeled separately with Alexa Fluor 555 using a kit (Microscale protein labeling kit; ThermoFisher Scientific; Waltham, MA, USA) and by following the manufacturer instructions ...
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bioRxiv - Biochemistry 2023Quote: ... HM and IF pooled EV fractions were assayed for total protein according to manufacturer’s instructions (Micro BCA protein assay kit, Thermofisher Scientific, Brisbane, Australia) and a volume equivalent to 20 µg total protein was aliquoted for western blot (WB ...
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bioRxiv - Cell Biology 2022Quote: ... For LI-COR westerns: The proteins were electro-transferred to nitrocellulose membranes and then the total protein was stained using Ponceau (Thermo Fisher Scientific). The membrane was then blocked with LI- COR blocking buffer at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... MSC-EVs were not lysed in RIPA buffer and protein content was determined via the Micro BCA™ Protein Assay Kit (ThermoFIsher, 23235).
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bioRxiv - Genetics 2022Quote: ... A protein concentration of the tissue lysates was determined with a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) and 3-10 µg of the total protein lysate/homogenate used per assay well in duplicates and repeated in three independent runs ...