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Citations for Thermo Fisher :
8751 - 8800 of 10000+ citations for West Nile Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The protein concentration of each lysate was determined by the Pierce BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA). Cell lysates (20 μg ...
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bioRxiv - Physiology 2023Quote: Cell surface proteins from transfected HEK293 cells were biotinylated using the Pierce Cell Surface Protein Isolation Kit (ThermoFisher Scientific., cat# 89881). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein concentrations in the supernatants were measured using the EZQ® Protein Quantitation Kit (Molecular Probes, Inc. Eugene, Oregon, USA) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were centrifuged at 20,000 x g for 10 min at 4 °C and protein concentrations were determined using Pierce BCA Protein Assay Kit (ThermoFisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at 13,000 rpm for 10 min at 4°C and the protein concentrations were quantified using Pierce BCA Protein Assay Kit (Thermo Fisher). Equal amounts of protein were separated on 8% SDS-PAGE and then transferred onto nitrocellulose membranes (Thermo Fisher) ...
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bioRxiv - Bioengineering 2023Quote: ... Values were normalized for total protein content in each well using a Pierce BCA Protein Assay Kit (Thermo Fisher, cat# 23225) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2022Quote: ... Twenty μL of the crude extract was removed for protein quantification by a Bradford protein assay according to manufacturer’s instructions (Thermo Scientific Inc.). Next ...
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bioRxiv - Biochemistry 2022Quote: ... and protein concentration was measured by the bicinchoninic acid (BCA) assay using the Pierce BCA protein assay kit (ThermoFisher Scientific: 23225). Equal amounts of protein were loaded on 4 to 12% SDS-PAGE gel and transferred onto nitrocellulose membrane ...
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bioRxiv - Biochemistry 2022Quote: ... and 150 µg of purified PPSA from SG and SEC fractions at 1 mg/mL total protein concentration (measured by a Pierce BCA protein assay, Thermo Scientific, according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein contents of total cell lysates were determined using a BCA protein assay kit (Thermo Scientific Pierce, Rockford, IL, USA). The equal amounts of protein were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Molecular Biology 2022Quote: Antibodies targeting proteins of interest or IgG isotype control (5ug) were incubated with 20ul of Dynabeads Protein G (Thermo Fisher Scientific) in buffer WB150 (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... Equal protein loads were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and proteins were transferred to nitrocellulose membranes (Thermo Scientific). For protein detection the following primary antibodies were used at the indicated dilutions ...
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bioRxiv - Microbiology 2023Quote: ... Protein concentrations were measured using a Qubit 2.0 Fluorometer with a Qubit Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol.
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bioRxiv - Plant Biology 2022Quote: ... The protein concentration of the supernatant was determined by bicinchoninic acid assay (Pierce™ BCA Protein Assay Kit, Thermo Fisher Scientific). 100 μg of protein were loaded into FASP filters (Amicon Ultra 30K ...
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bioRxiv - Microbiology 2022Quote: ... Samples were filtered through nitrocellulose membranes to remove the beads and protein was quantified using a Qubit 2.0 fluorometer and a Qubit™ protein assay kit (Life Technologies). 50 μg of protein sample was suspended in 50 μl of 8 M urea buffer ...
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bioRxiv - Plant Biology 2022Quote: ... The molecular weight of protein bands was estimated by comparison with the BenchMark™ Pre-stained Protein Ladder (Thermo Fisher Scientific). The abundance of the 3xFLAG-ZF proteins were quantified from the band intensities also using Image Lab Software ...
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bioRxiv - Molecular Biology 2023Quote: ... protein in the supernatants was quantified using Pierce™ Bovine Serum Albumin (BCA) Protein Assay Kit (Thermo Fisher Scientific, Cat. 23225).
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bioRxiv - Microbiology 2023Quote: ... a volume equivalent to 250 µg protein (Quantification: 660 nm Protein Assay with ionic detergent compatibility reagent; Thermo Scientific, 22660 & 22663) was transferred to 15 mL centrifugation tubes and filled up with 1% SDS in 50 mM Na2HPO4 to a total volume of 920 µL ...
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bioRxiv - Genomics 2023Quote: ... Eluted proteins and 50 μg of protein from whole cell lysate were resolved in 4-15% NuPAGE gradient gel (ThermoFisher, NP0335) using MOPS running buffer (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: Cell Lysis Buffer (CST) was used to extract protein lysates and the Subcellular Protein Fractionation Kit for Cultured Cells (ThermoFisher Scientific) was used for fractionation according to manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... An aliquot (25□l) of this lysate was used for protein quantitation using Pierce Coomassie protein assay kit (cat#23200, ThermoFisher scientific). The remaining lysate was centrifuged at 5000xg at 4C to pellet debris ...
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bioRxiv - Cell Biology 2023Quote: ... The collected supernatant was used to measure protein concentration using the BCA assay (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was used to determine the protein concentration by BCA assay according to the manufacturer’s instructions (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific).
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bioRxiv - Genetics 2023Quote: Cultured human iPSC-derived NGN2 neurons were maintained in 6-well plates and were lysed on day 21-24 for membrane protein extraction using the Mem-PER Plus Membrane Protein Extraction kit (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... Protein concentrations in the supernatant were determined using the Pierce Micro BCA Protein Assay kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: ... Protein levels of the brain tissue were measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Cell Biology 2023Quote: ... Debris was removed by centrifugation (4°C, 30 min, 20’000 g) and total protein concentrations measured using the Bradford protein assay (Thermo Fisher Scientific). Typically ...
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bioRxiv - Plant Biology 2023Quote: ... The homogenate was centrifuged and the protein concentration of the supernatant was measured using a Pierce 660 nm Protein Assay Reagent (Thermo Scientific). Thirty-five microgram of protein was loaded in each lane ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was collected after centrifuging at 13,000 RPM (10 mins, 4°C) and total protein was estimated by BCA (Pierce BCA Protein Assay Kit, ThermoFisher Scientific, 23225). The supernatant was mixed with 1X Laemmli buffer and boiled at 95°C for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... The same amount of protein was used for the enrichment of biotinylated proteins using100 μl Pierce™ streptavidin agarose beads (Cat. # 20353, ThermoFisher) overnight at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... Supernatants were collected and protein concentration was determined using a Pierce™ BCA Protein Assay Kit (Cat#: 23225, Thermo Fisher scientific) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Total proteins from intestinal epithelial cells or intestinal organoids were extracted with M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific) with proteinase inhibitor (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... For normalization total protein concentration was measured on an aliquot of the cell lysate with the Pierce BCA protein kit (Thermo Fisher) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 μg of antibodies against Piwi and Aubergine were each coupled to 50:50 of protein-A and protein-G Dynabeads (Life Technologies). Lysates were incubated with bead-coupled antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... Antibodies were captured by 20 µ l of a 50:50 mix of Protein-A and Protein-G Dynabeads (Life Technologies). The beads were washed several times sequentially in the buffers containing low salt (150 mM NaCl) ...
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bioRxiv - Cell Biology 2023Quote: ... samples were collected by centrifugation (11,000 ×g, 20 min) and their protein content was determined (Thermo Pierce BCA Protein Assay kit, ThermoFisher, Cat# 23225). Proteins (∼30 µg/lane ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein lysate from each sample was collected and the concentration of the protein was estimated using Pierce™ BCA Protein Assay Kit (ThermoFisher). For each sample ...
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bioRxiv - Immunology 2023Quote: ... Bound immune complexes were captured with an equal mix of protein A and protein G Dynabead magnetic beads (Thermo Fisher Scientific), which recognize all mouse IgG subclasses ...
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bioRxiv - Genetics 2023Quote: ... The concentration of the liberated soluble protein was assessed with the Pierce BCA protein assay kit (Thermo Fisher, Waltham, MA, USA), and equal amounts of protein (180-250 µg ...
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bioRxiv - Immunology 2023Quote: ... Protein concentration of the supernatant was determined by following manufacturers instruction for the Pierce BCA Protein Assay Kit (ThermoFisher-Scientific #23227). Once total protein concentration was determined ...
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bioRxiv - Biochemistry 2023Quote: Purity of all proteins was verified by SDS-PAGE and concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Biochemistry 2023Quote: Purity of all proteins was verified by SDS-PAGE and concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Molecular Biology 2023Quote: The accumulation of DNA damage response proteins to chromatin following IR-induced DNA damage was examined using the Subcellular Protein Fractionation Kit (Thermo Fisher) as previously described (23) ...
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bioRxiv - Molecular Biology 2024Quote: ... Total protein concentration in the supernatant was determined using the Micro BCA protein assay kit (Thermo Scientific, Waltham, MA; Cat.: 23235). In the same supernatant ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were lysed at 4°C with RIPA buffer and protein concentration was determined with the Micro kit BCA Protein Assay (Thermo Scientific, Courtaboeuf ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentration was measured to be 0.1 mg/ml by the BCA protein assay kit (Thermo Fisher Scientific Inc., Waltham, MA). To make cryo-specimen of Fab fragment ...
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bioRxiv - Molecular Biology 2023Quote: ... The cell lysate was then sonicated and centrifuged at 16,000g for 15min and the supernatants were extracted for protein concentration measurement by Pierce BCA protein assay (ThermoFisher, Cat# 23227). For the samples of differentiation time course ...
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bioRxiv - Bioengineering 2023Quote: Protein contents of the SEC fractions were measured using a BCA protein assay kit (23225, Pierce, Thermo Fisher Scientific, MA, USA). A standard curve (range 0–1000 μg/ml ...
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Volumetric Compression Shifts Rho GTPase Balance and Induces Mechanobiological Cell State TransitionbioRxiv - Biophysics 2023Quote: ... A small aliquot of cell lysate was later thawed and used for total protein quantification following the “BCA Protein Assay Kit” manual (Thermo Scientific). The same quantity (60 μL ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were harvested and lysed prior to splitting each sample into two aliquots for D-2-HG quantification and total protein quantification using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...