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Citations for Thermo Fisher :
8851 - 8900 of 10000+ citations for West Nile Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Samples were centrifuged at 10,000 x g for 30 min at 4 °C before supernatants were collected and protein content was determined using the BCA Protein Assay Kit (Pierce, Thermo Fisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2019Quote: ... to remove RNA and DNA and then incubated overnight with the appropriate antibody and then incubated overnight with a 50:50 mixture of protein A and protein G Dynabeads (Thermo Fisher Scientific). Beads were washed three times with nondenaturing lysis buffer (20 mM Tris-HCl ...
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bioRxiv - Genetics 2019Quote: ... The molecular migration position of transferred proteins was compared to the PageRuler Prestain Protein Ladder 10 to 170 kDa (Thermo Fisher Scientific). Signals were detected using the Chemidoc XRS+ imaging system (Biorad ...
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bioRxiv - Cancer Biology 2019Quote: ... we transferred the supernatant to a clean tube and determine the protein concentration using the EZQ® Protein Quantitation Kit (Cat. no. R33200,Thermo Scientific) or another method ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: We quantified the protein content in the clarified lysates of the damselflies using Pierce™ BCA protein assay kit (Thermo Fisher Scientific). We used the Bovine Serum Albumin (BSA ...
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bioRxiv - Physiology 2020Quote: ... and MOTS-c in each group was normalized to the total tissue protein concentration as determined by the Bradford protein assay (Thermo Fisher Scientific).
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bioRxiv - Physiology 2020Quote: ... Protein pellets from each aliquot per sample were pooled and the total protein content were measured using BCA protein Assay kit (ThermoFisher Scientific, Australia). The protein disulphide bonds were reduced with 10 mM dithiothreitol for 30 min at 60 °C and the cysteine residues were alkylated with 20 mM iodoacetamide for 30 min at 37 °C in the dark ...
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bioRxiv - Cell Biology 2019Quote: ... The lysate was centrifuged at (21000 g) for 15 min at 4°C and protein concentration determined (Pierce™ 660nm Protein Assay Reagent, Thermo Scientific). 8.5 μg lysate was kept for western blot analysis and 1.6-1.8 mg total protein was added to 250 μl washed high capacity neutravidin beads (Life Technologies ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Protein content in each sample was measured with NanoOrange Protein Quantification Kits according to the manufacturer’s instructions (Thermo Fisher Scientific, Waltham, MA), and was used for ATP synthesis rate normalization ...
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bioRxiv - Biophysics 2019Quote: ... the final dye to protein ratio was typically 5-6 dye molecules per protein molecule as measured with a Nanodrop spectrophotometer (Thermo Fisher Scientific). Cells in suspension culture dishes were labeled with 0.5 nM fluorescent IgE for typically 12-20 hours before imaging.
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bioRxiv - Bioengineering 2019Quote: Proteins were collected 48 hours post-transfection and their concentrations were measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Western blots were performed as described previously (Lee et al ...
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bioRxiv - Pathology 2019Quote: ... Protein concentration of the supernatant was determined using the BCA assay (Pierce™ BCA Protein Assay Kit, ThermoFisher Scientific, cat. number 23225). Samples were mixed with SDS-loading buffer without heating and electrophoretically separated on a 4–20% Criterion™ TGX™ Precast Midi Protein Gel (Bio-rad ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein extracts were diluted in PBS and quantified in duplicate using a Micro BCA Protein Assay Kit (ThermoFisher Scientific, cat no: 23235). Bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were washed once with ice-cold PBS and protein lysates were extracted using M-PER mammalian protein extraction reagent (Thermo Fisher Scientific) with protease and phosphatase inhibitors (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... and searched against the UniProt Human protein database and RefSeq SARS-CoV-2 protein database using Sequest HT through Proteome Discoverer (Version 2.2) (Thermo Scientific, Bremen, Germany). Precursor and fragment mass tolerance were set to 10 ppm and 0.02 Da ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatants containing soluble protein were obtained and the concentration of protein determined using A280 readings (Thermo Scientific NanoDrop 2000/2000c spectrophotometer). Poly-G17U and CR1 labeled with a 3’ fluorescein (Dharmacon ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatants containing soluble protein were obtained and the concentration of protein determined using A280 readings (Thermo Scientific NanoDrop 2000/2000c spectrophotometer). Fluorescein-labeled Poly-G17U ...
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bioRxiv - Bioengineering 2020Quote: ... Intensity quantification was performed using ImageJ software and the obtained values were corrected by culture media background (without having been in contact with cells) and total protein content measured using the Pierce™ BCA Protein Assay Kit (Thermo Scientific). Values were further normalized by internal negative and positive controls from the antibody assay ...
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bioRxiv - Neuroscience 2020Quote: ... 10μg protein was loaded per well on 8% acrylamide gels with prestained standard molecular weight markers (PageRuler prestained protein ladder; Thermo Fisher Scientific) and run at 60mV for 3 hours using the mini Protean II gel electrophoresis apparatus (Bio-Rad Laboratories) ...
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bioRxiv - Neuroscience 2021Quote: ... We used 30 μL of the preparation for HPLC characterization and another 30 μL for protein dosage by the BCA method (Pierce BCA protein assay – Thermo Fisher Scientific). Finally ...
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bioRxiv - Cancer Biology 2021Quote: ... A total amount of 30 µg protein was loaded per lane of NuPAGE Tris-Acetate Protein Gel 3-8% (Life Technologies, EA0375BOX) and separated according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were centrifuged at 14000 rpm for 15 minutes at 4°C and protein concentration determined with a Pierce™ BCA Protein Assay Kit (Life Technologies). High capacity neutravidin beads (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... centrifuged at 12,000 x g at 4°C for 15 min and the supernatant containing the nuclear proteins was transferred into fresh tubes and quantified using the BCA protein assay (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2020Quote: ... Nuclear membrane and debris were pelleted by centrifugation and protein concentration of the cleared lysate was determined with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). 2μg of the anti-BAF155 antibody (ab172638 ...
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bioRxiv - Biochemistry 2021Quote: ... MS1 and MS2 masses were recalibrated using an external calibrant mixture of intact proteins (PiercePierce™ Intact Protein Standard Mix, Thermo Scientific) measured before and after each HTT sample ...
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bioRxiv - Biochemistry 2020Quote: ... The cell debris was removed by centrifugation and the protein concentration of the supernatants were determined using Pierce™ bicinchoninic acid (BCA) protein assay kit (Thermo Scientific) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Challenged A549 cells were lysed with modified RIPA buffer (Supplementary Data 3) and protein concentration was determined using a bicinchoninic acid (BCA) protein assay (Thermo Fisher Scientific). Phospho-p38 (p-p38) ...
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bioRxiv - Microbiology 2021Quote: ... followed by collection of supernatants for quantification of total protein concentration using Microplate BCA Protein Assay Kit (Pierce™, Thermo Fisher Scientific). Equal amounts of protein were separated by SDS-PAGE using 4-15% SDS polyacrylamide gels (Bio-Rad) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and the supernatant was transferred to fresh tubes and total protein concentration was equalized using Pierce 660-nm protein assay reagent (22660, Thermo Fisher Scientific). 5% of equalized lysates were taken out before pull-down to analyze the biotinylation of inputs by western blot analyses ...
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bioRxiv - Bioengineering 2019Quote: ... The tissues were dissolved in RIPA buffer and the total protein concentration was measured with a Pierce BCA protein assay kit (Thermo Fisher Scientific). Proteins (15 μg each ...
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bioRxiv - Systems Biology 2020Quote: ... Lysates were clarified by centrifugation at 10,000 rpm for 2 minutes at 4°C and snap frozen in liquid nitrogen with protein concentrations measured using the BCA protein assay (Pierce/Thermo Fisher Scientific). For RAS activation assays ...
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bioRxiv - Cell Biology 2020Quote: ... was solubilized with 0.1 N NaOH and protein concentration was determined using Pierce™ BCA Protein Assay Kit (Thermo Fisher, Waltham, MA). Cellular cholesterol content data was expressed as mg cholesterol/mg of protein ...
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bioRxiv - Biophysics 2020Quote: ... For more accurate characterization protein concentration was determined by the bicinchoninic acid method31 with the Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific). A method based on pyridine hemochrome spectra32 was used to determine the amount of heme ...
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bioRxiv - Biophysics 2020Quote: ... proteins were labeled using Alexa-fluor 488™(green) or Alexa-fluor 594™(red) microscale protein labeling kits (ThermoFisher Scientific, Invitrogen). Small amounts (∼0.3 µl ...
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bioRxiv - Biophysics 2020Quote: ... proteins were labeled using Alexa-fluor 488™(green) or Alexa-fluor 594™(red) microscale protein labeling kits (ThermoFisher Scientific, Invitrogen). Small amounts (∼0.3 µl ...
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bioRxiv - Cell Biology 2021Quote: ... Media were centrifuged to remove cellular debris and the protein concentration was determined with the bicinchronic acid (BCA) protein assay (ThermoFisher Scientific, www.thermofisher.com). Following separation of proteins by SDS-PAGE ...
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bioRxiv - Cell Biology 2021Quote: ... Fibroblast lysates were centrifuged to remove insoluble material and protein concentration of the supernatants determined with the bicinchronic acid (BCA) protein assay (ThermoFisher Scientific, www.thermofisher.com). Following separation by sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were then centrifuged at 13,500 rpm for 10 min at 4°C and then rotated with 10 μl of pre-equilibrated protein A beads per sample (Pierce Protein A Magnetic Beads, ThermoFisher 88845, RA228548) for 1 h at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... and the contrast value of each protein molecule was converted to mass with calibration obtained from an unstained protein ladder (LC0725, Thermofisher, Wattham, MA).
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration of each lysate was measured using the BCA protein assay kit according to manufacturer’s directions (Thermo-Fisher Scientific, Waltham, MA). Proteins were separated on a 10% SDS-PAGE gel with equivalent amounts of protein loaded in each lane (Thermo-Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... 10-20 μg of purified proteins were separated by SDS-PAGE and stained with mass spectrometry compatible Imperial protein stain (Thermo Fisher Scientific). For the identification of phosphorylation site(s ...
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bioRxiv - Microbiology 2022Quote: ... China).The purity of the protein was assessed by SDS-PAGE gel and its concentration was determined using the BCA Protein Assay Reagent (Thermo Fisher Scientific). AdpA cysteine mutant proteins (C62S ...
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bioRxiv - Genetics 2022Quote: Total protein was isolated from kidneys or cells using lysis buffer made from mixing T-PER tissue protein extraction reagent (Invitrogen, catalog# 78510) with a protease phosphatase inhibitor tablet (Fisher ...
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bioRxiv - Microbiology 2022Quote: ... Cleavage of the spike protein was detected using anti-SARS-CoV Spike Protein (SDelta3) monoclonal antibody (7G12, Thermo Fisher Scientific, CA, USA)) raised against immunogen aa168-461 of the S1 domain.
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bioRxiv - Biochemistry 2022Quote: ... Protein concentrations were determined using the Thermo Scientific™ Pierce™ BCA (bicinchoninic acid) Protein Assay Kit (Thermo Scientific™ Cat# 23225). Following concentration determination ...
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bioRxiv - Neuroscience 2021Quote: ... Antibody-bound NLGNs were incubated for 1 hour with 20 µL of protein G beads (Dyna-beads Protein G, Thermo Fisher Scientific) precipitated and washed 4 times with lysis buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Lysates were passed through a 0.22 μm Spin-X filter column after which total protein amount was determine using the Pierce BCA Protein Assay Kit (#23225, Thermo Fisher Scientific). Equal protein amounts were denatured in SB and subjected to SDS-PAGE ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations in the tissue lysates were measured with the aid of a bicinchoninic acid protein assay kit (Thermo Scientific, Rockford, IL). TNF-α ...
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bioRxiv - Microbiology 2020Quote: ... The supernatants were transferred to new ice-cold Eppendorf tubes and protein concentration was measured by the BCA assay using Pierce BCA Protein Assay kit (ThermoFisher Scientific; #23225). Equal amounts of protein were loaded on 4-12% SDS-PAGE gel and transferred onto nitrocellulose membrane ...
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bioRxiv - Microbiology 2021Quote: ... microsphere mixtures were successively incubated with serum samples (1:400) biotinylated protein A and biotinylated protein G (4 μg/ml each) (Thermo Fisher Scientific), and Streptavidin-R-Phycoerythrin (4 μg/ml ...