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Citations for Thermo Fisher :
851 - 900 of 10000+ citations for CD8 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... hiPSCs were cultured on human recombinant vitronectin in StemFlexTM media (Life Technologies) and differentiation was initiated by plating singularised hiPSCs on human embryonic stem cells (hESC)-qualified Matrigel (Corning ...
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bioRxiv - Immunology 2023Quote: ... Recombinant human IFN-β (1000 U/ml to 1 U/ml, ThermoFisher) was used as positive controls for IRF activation ...
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bioRxiv - Cancer Biology 2023Quote: ... Cholera Toxin Subunit B (Recombinant) Alexa Fluor 488TM Conjugate (C34775, Invitrogen, Belgium), Tetramethylrhodamine ...
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bioRxiv - Plant Biology 2023Quote: ... 2 μL of RNaseOUT™ Recombinant RNase Inhibitor (40 units/μL, Invitrogen) and 2 μL of SuperScript™ III RT (200 units/μL ...
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bioRxiv - Synthetic Biology 2023Quote: All recombinant proteins were produced in Escherichia coli BL21(DE3) pLysS (Invitrogen) transformed with respective plasmids ...
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bioRxiv - Cell Biology 2024Quote: ... 20 % FBS and 200 ng/mL Human M-CSF Recombinant Protein (ThermoFisher). Six days after the initial isolation ...
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bioRxiv - Microbiology 2024Quote: ... rabbit (goat anti-rabbit IgG, Invitrogen), sheep (rabbit anti-sheep IgG ...
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bioRxiv - Neuroscience 2020Quote: ... anti-IFN-γ neutralizing mAb and/or an isotype IgG1 (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) were intraperitoneally injected daily to mice from PND5 to PND10 ...
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bioRxiv - Microbiology 2022Quote: ... The V5 epitope was detected with mouse mAb anti-V5 (diluted 1:1000) (Invitrogen, catalong no. R96025). Toxoplasma-specific antibodies include mouse mAb m-IMC1 (diluted 1:500 ...
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bioRxiv - Microbiology 2021Quote: ... anti-Utr2 mAbs or a commercially sourced anti-Candida mouse IgG2a monoclonal antibody (MA1-7009) (Fisher Scientific) in pre-warmed supplemented CO2-independent medium and incubated at 37 °C with gentle shaking for 40 min.
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bioRxiv - Biochemistry 2021Quote: ... The endoplasmic reticulum was stained using an Alexa488-coupled anti-calnexin (1:200, mAb AF18, ThermoFisher Scientific) in 10% goat serum overnight at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... The concentration of the purified mAb was determined using the Coomassie Plus protein assay reagent (Thermo Scientific).
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bioRxiv - Microbiology 2021Quote: ... Oregon green 488 was first conjugated to mAb 18B7 using Oregon Green 488 Protein Labeling Kit (ThermoFisher). BMDMs were plated at a density of 1.25 × 105 cells/well on 24-well plate with 12 mm circular coverslip ...
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bioRxiv - Immunology 2021Quote: ... The following primary antibodies (Ab) were used: occludin (mouse monoclonal [mAb], clone OC-3F10, Thermo Fisher Scientific), TFF3 (rabbit polyclonal [pAb] ...
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bioRxiv - Microbiology 2022Quote: Anti-ORF8 mAb (#1-3-2) was coupled to aldehyde/sulfate latex beads (Thermo Fisher Scientific A37304). The antibody was mixed with the latex beads and shaken at room temperature overnight ...
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bioRxiv - Microbiology 2023Quote: ... Blots were then incubated (1:500 dilution) with mouse anti-human E-cad mAb (4A2C7, Life Technologies) and anti-human HtrA (PA523395 ...
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bioRxiv - Microbiology 2023Quote: ... they were stained with a mastermix of anti-cytokine/transcription factor mAbs containing BCL6 PE (ThermoFisher Scientific) at 4 °C for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: The following primary antibodies were used: anti-ezrin mouse monoclonal antibody (mAb) (1:500; MA5-13862, Invitrogen); anti-p75 NTR rabbit mAb (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were produced at MAB Discovery GmbH using the FreeStyle™ 293 expression system from Thermo Fisher. Transfected HEK-293 FreeStyle™ cells were incubated for 10-11 days at 37°C ...
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bioRxiv - Immunology 2023Quote: ... The assay for MUC5AC was performed using biotin-conjugated mouse anti-MUC5AC mAb (45M1, Invitrogen, #MA5-12175) as described previously (12) ...
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bioRxiv - Cancer Biology 2023Quote: ... the TCO- or Tz-conjugated mAbs were then labeled with Alexa Fluor 488 (Thermo Fisher Scientific, A2042), Alexa Fluor 594 (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... The 3 mAbs were specifically conjugated to HRP using EZ-Link™ Plus Activated Peroxidase Kit (ThermoFisher) and detected with ECL (Pierce ...
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bioRxiv - Immunology 2022Quote: ... incorporation of glucose and fatty acid metabolism was determined by flow cytometry analysis of isolated CD8+ T cells from PLWH and control donors stained with 2-NBDG (Invitrogen) and 500/510 BODIPY (Molecular Probes ...
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bioRxiv - Immunology 2021Quote: ... cells were stained with anti-CD4-e450 (eBioscience) and anti-CD8-FITC antibody (eBioscience) followed by incubation with 100 µM mBcl (Monochlorobimane, Invitrogen) as described (Jayaraman and Jayaraman ...
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bioRxiv - Immunology 2019Quote: Intracellular ATP levels were measured ex vivo on sorted CD4+ and CD8+ T cell subsets via a bioluminescence assay according to the manufactures instructions (ThermoFisher).
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bioRxiv - Immunology 2021Quote: ... Naïve CD8+ T cells were activated for two days with Human T-Activator CD3/CD28 Dynabeads (ThermoFisher Scientific, Waltham, MA). After two days ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 million CD8+ or CD4+ T cells were stained with 0,07 uM CellTrace Far Red Cell Proliferation dye (ThermoFisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Day 12 CD8 CAR T cells TM-LCL co-cultures were stained with LIVE/DEAD Fixable Aqua Dead Cell Stain (ThermoFisher) followed by antibody staining against CD8 (FITC ...
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bioRxiv - Immunology 2020Quote: ... naïve CD8+ T-cells were isolated from an enriched PBMC fraction and activated with αCD3 and αCD28 beads (Dynabeads, Invitrogen) for 48 hours ...
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bioRxiv - Immunology 2019Quote: ... Purified OTI CD8+cells or whole splenocytes from OT1 mice were labeled with Cell Proliferation Dye eFluor™ 450 (Invitrogen). After discarding the supernatant ...
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bioRxiv - Bioengineering 2019Quote: CD8+ and CD4+ T cells were purified from PBMC and activated by stimulation with anti-CD3/anti-CD28 beads (Thermofisher) in separate cultures ...
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bioRxiv - Immunology 2019Quote: ... Colonic lymphocytes were used without prior enrichment by CD8 microbeads and were maintained in R10 supplemented with 25ng/mL amphotericin B (Gibco), 40μg/mL gentamicin (Gibco) ...
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bioRxiv - Genetics 2019Quote: ... Non-adherent cells in suspension from resting and PHA-stimulated cultures were removed and purified for CD8−/CD4+ T cells using Dynabeads (Invitrogen) and stored in RNAprotect Cell reagent (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... non-naïve (CCR7+CD45RA-, CCR7-CD45RA- and CCR7-CD45RA+) CD3+CD8+ T cells were directly sorted into TRIzol reagent (Thermo Fisher) on a FACSAriaFusion (BD ...
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bioRxiv - Bioengineering 2021Quote: ... isolated CD8 T cells were cultured in T cell media [complete RPMI media supplemented with 1X nonessential amino acids (Gibco) + 1 × 10−3 M sodium pyruvate (Gibco ...
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bioRxiv - Immunology 2021Quote: ... Live CD3/CD8-CD14-CD19+IgM-IgD-IgG+Spike+Spike+ cells were sorted into individual wells containing RNase OUT (Invitrogen), First Strand SuperScript III buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... CD8+ T cells were activated with Dynabeads Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation (Gibco, 11456D) without exogenous recombinant IL2 supplementation ...
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bioRxiv - Immunology 2022Quote: ... freshly isolated or cryopreserved human PBMC or purified CD8+ T-cells (1×106) were cultured in complete culture medium (RPMI 1640 (Gibco) supplemented with 10% FCS ...
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bioRxiv - Microbiology 2023Quote: ... the infected cells were co-cultured with HLA-B/CMV-restricted CD8+ T-cells for five hours (E:T ratio: 3:1) in RPMI 1640 medium (Life Technologies)(supplemented with 10 % (v/v ...
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bioRxiv - Cancer Biology 2022Quote: ... 10×105 naïve CD8+ T cells were seeded onto mouse tumor cell lines and maintained in RPMI 1640 media (Thermofisher, 11875093) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... Nuclear and cytoplasmic extracts from CD8+ T cells were obtained with the NE-PER Nuclear and Cytoplasmic Extraction Reagents (78833, ThermoFisher). The Revert 700 Total Protein Stain (LICOR ...
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bioRxiv - Immunology 2023Quote: ... and CD8+ cells were combined at a 1:1 ratio and activated using anti-human CD3/CD28 Dynabeads (Invitrogen, #40203D) at a 3:1 ratio ...
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bioRxiv - Bioengineering 2023Quote: ... 105 rested NY-ESO-1 TCR+ T cells (4:1 CD8+:CD4+) were labeled with 0.5uM CSFE (Thermo Fisher Scientific) and co-cultured with 105 NucLight red+ tumor cells in complete medium ...
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bioRxiv - Cancer Biology 2023Quote: ... sections were boiled in sodium citrate buffer (pH 6.0) for 15 min and incubated with the following antibodies for 1h: anti-CD8 (4SM15, Thermo Scientific), anti-NCR1 (EPR23097-35 ...
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bioRxiv - Immunology 2024Quote: ... staining was performed in the presence of 50 nM dasatinib at a 1:75 dilution and anti-CD8 antibody clone KT15 (ThermoFisher) was used to minimize background signal ...
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bioRxiv - Immunology 2024Quote: ... sections were blocked with 5% mouse serum and labelled using anti-mouse CD8 (clone 4SM15, Invitrogen cat 14-0808-82) followed by anti-rat IgG Alexa555 ...
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bioRxiv - Immunology 2024Quote: ... fraction in MACS buffer (2% heat-inactivated FBS, 2% 0,1M EDTA in PBSO) using the CD8+ Dynabead isolation kit (Thermo Fisher) and BD IMag Cell Separation Magnet (BD Biosciences) ...
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bioRxiv - Cell Biology 2020Quote: ... and recombinant SHH was purified with Detoxi-Gel™ Endotoxin Removing Gel (ThermoFisher) and dialyzed ...
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bioRxiv - Plant Biology 2020Quote: All recombinant plasmids were generated by the GATEWAY cloning system (Invitrogen, http://www.invitrogen.com/). The primers used for gene amplification are listed in S3 Table ...
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bioRxiv - Developmental Biology 2021Quote: ... Recombinant lentivirus was produced by transfecting HEK293T cells with Lipofectamine 2000 (Invitrogen, 11668019) or TransIT-2020 transfection reagent (Mirus ...