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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for CD8 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Recombinant proteins were diluted in DPBS (Life technologies cat. # 14040-182) (tetNA was diluted to 0.5 µg/ml) ...
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bioRxiv - Cell Biology 2020Quote: ... PRC1 (mouse mAb IgG2b, clone 16F2, product MA1-846; used at 1/100) was from ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Primary Strep-MAb classic chromeo-488 (IBA) and secondary Alexa-fluor 488 goat anti-mouse (Invitrogen) were applied sequentially with PBS washes in between ...
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bioRxiv - Immunology 2021Quote: ... Serial dilutions of the mAb samples were made in 1X minimal essential medium (MEM; Life Technologies) starting at 30 µg/ml ...
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bioRxiv - Microbiology 2021Quote: ... 5 ul of mAb DG52 pre-coupled to 100 μl Dynabeads™ Protein G (Thermo Fisher) was added to the supernatant and incubated for 1 hr at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... Chambers were washed 3X with MAB and incubated with anti-mouse Alexa647 (A21236, Thermo Fisher Scientific) secondary antibody for 1.5 hours ...
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bioRxiv - Microbiology 2020Quote: ... The mouse mAb against cathepsin L (BMS1032) and α-tubulin (T5158) were bought from Thermo Fisher Scientific and Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Goat anti-rat and streptavidin Molecular Probes Alexa-Fluor-488 and 633 conjugated secondary mAbs (Invitrogen) were used at 2µg/ml and all sections were counterstained with DAPI to distinguish cell nuclei ...
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bioRxiv - Immunology 2021Quote: The IgGs representing the mAbs were produced in either HEK 293T (ATCC) or Expi293 (Thermo Scientific) cells ...
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bioRxiv - Microbiology 2021Quote: ... monoclonal anti-HAtag antibody (1:8000; HA Tag mAb 2-2.2.14, Thermo Fisher Scientific, Planegg, Germany) or COVID-19 patient serum (1:200) ...
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bioRxiv - Immunology 2021Quote: ... The IC50 of the evaluated mAbs was tested by the Varioskan LUX Microplate Spectrophotometer (Thermo Fisher), and calculated by a four-parameter logistic regression using GraphPad Prism 8.0.
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bioRxiv - Cell Biology 2022Quote: ... The rat PE-conjugated mAb against F4/80 (catalog #12-4801-82) was from Thermo Fisher. The rabbit polyclonal anti-mouse αM antibody (catalog #ab128797 ...
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bioRxiv - Microbiology 2023Quote: ... followed by an incubation with mouse anti-human E-cad cytoplasmic domain mAb (4A2C7, Life Technologies). In some experiments ...
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bioRxiv - Immunology 2023Quote: ... Myc mAb (D84C12) was labeled by Alexa Fluor™ 647 Antibody Labeling Kit (Invitrogen, Cat# A20186). Surfaced staining was conducted using Cell Staining Buffer (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... mAbs were expressed by transient transfection in Expi293F cells in FreeStyle F17 expression media (Thermo Fisher) (0.1% Pluronic Acid F-68 and 20% 4 mM L-glutamine ...
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bioRxiv - Immunology 2024Quote: ... AnnexinV/7ADD (eBiosciences) and Fas Ligand Ms Anti-Hu mAb-FITC (SB93a, 1:200, Life Technologies). For the characterization of primary macrophages ...
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bioRxiv - Bioengineering 2024Quote: ... Tregs were stained with conjugated mAb targeting the intracellular Helios (eBiosciences, Life technologies corp., Carlsbad, CA) and FoxP3 proteins (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... Anti-flavivirus E protein mAb 4G2 was conjugated to Alexa Fluor 488 5-SDP (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Proliferation was measured by labelling primary CD8+ T-cells with 1 μM carboxyfluorescein diacetate succinimidyl ester (CFSE, Thermo Fisher) for 10 min at 37°C before activation and treatments ...
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bioRxiv - Immunology 2022Quote: ... CD4+ and CD8+ T-cells were stained with LysoTracker Red DND-99 (Invitrogen; 5 μM; 1 h; 37°C), fixed (3% PFA ...
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bioRxiv - Immunology 2022Quote: ... cells at 30 h (CD8+T) or 48 h (CD4+ T) after stimulation were permeabilized using Cytofix/Cytoperm (Invitrogen) and stained with a mixture of anti-Ki-67 antibody and NucSpot Far-Red for 15 min at room temperature in the dark ...
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bioRxiv - Immunology 2020Quote: ... Isolated T cells (including CD4+ and CD8+) were CFSE labeled by incubating them with 80 μm CSFE (Thermo Fisher) in CCM for 5 min at RT as described previously58 ...
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bioRxiv - Cancer Biology 2021Quote: ... CD8 and FoxP3 a red fluorescence Alexa Fluor 594 donkey anti-mouse IgG (Thermo Fisher Scientific, Germany; A-21203) was used ...
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bioRxiv - Immunology 2020Quote: ... Primary human CD8+ T cells were cultured for up to 24 days in ATCC-modified RPMI (Thermo Fisher, #A1049101) supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... live CD3+CD8+) were sorted from a C57BL/6Thy1.1 mouse as responder cells and labeled with 5 μM CellTrace Violet (Invitrogen). Whole splenocytes from 6-8 week-old ...
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bioRxiv - Immunology 2021Quote: ... FACS-sorted CD4+CD8−CD25−CD62L+CD44lo naïve T cells were seeded in T cell medium [RPMI 1640 (Gibco), 10% heat-inactivated FBS (Atlantis) ...
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bioRxiv - Immunology 2023Quote: ... Purified naïve 8.3 CD8 T cells were labeled using a CellTrace violet cell proliferation kit (Invitrogen, Waltham, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... stained with cell surface antibodies at 1:1200 (anti-CD8: PerCP-Cyanine5.5, eBioscience, # 45-0081-82, or Biolegend, #100734, anti-CD44: APC, Invitrogen, #17-0441-82 ...
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bioRxiv - Immunology 2023Quote: ... Isolated CD8+ T cells were cultured for 72 hours in T cell culture media: RPMI (GIBCO, Cat#11875-093) supplemented with 10% of heat-inactivated FBS ...
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bioRxiv - Immunology 2024Quote: CD4+ and CD8+ T cells were combined 1:1 and stimulated with CTS™ Dynabeads™ CD3/CD28 (Invitrogen) at a 1:1 ratio overnight in X Vivo-15 (Lonza ...
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bioRxiv - Immunology 2024Quote: ... and naïve CD8 T cells from OT-I donors were isolated using the Dynabeads Untouched Mouse T Cells kit (Invitrogen), both according to manufactures protocol ...
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bioRxiv - Plant Biology 2019Quote: ... The recombinant enzymes were probed using anti-V5-HRP conjugated antibody (Invitrogen), which was detected using an ECL Advance Western Blotting Detection Kit (Amersham ...
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bioRxiv - Immunology 2021Quote: ... and recombinant proteins were purified using Ni-NTA agarose (Thermo Fisher Scientific), then buffer-exchanged into PBS and concentrated using Amicon Ultra centrifugal filters (MilliporeSigma) ...
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bioRxiv - Molecular Biology 2020Quote: Recombinant 6x-His tagged PTB was purified using Ni-NTA agarose (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Recombinant BUD13 purification was confirmed by SimplyBue SafeStain (Thermo Fisher Scientific, LC6060) and western blot using BUD13 antibody (Bethyl Laboratories ...
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bioRxiv - Immunology 2021Quote: All recombinant proteins were expressed and purified using Expi293F cells (Life Technologies,) as described in detail previously [34 ...
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bioRxiv - Immunology 2019Quote: ... slices were overlaid with 50ng recombinant IL-15/IL-15Rαcomplex (Thermo Fisher) in 10□1 of cRPMI following peptide treatment ...
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bioRxiv - Biochemistry 2019Quote: ... Recombinant virus was made by co-transfection into SF9 insect cells (Invitrogen) of the plasmid and BacVector3000 baculovirus DNA (Novagen ...
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bioRxiv - Molecular Biology 2019Quote: ... and 2) 1 U/μL RNaseOUT™ Recombinant Ribonuclease Inhibitor (ThermoFisher Scientific). Hydra polyps in MCB buffer were homogenized on ice using a Dounce homogenizer ...
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bioRxiv - Neuroscience 2019Quote: ... and 20 ng/mL recombinant human fibroblast growth factor-basic (Life Technologies). After 3 to 6 weeks ...
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bioRxiv - Immunology 2019Quote: ... recombinant macrophage colony-stimulating factor (100 U/ml; ebioscience, Thermo Fisher Scientific) and Pen Strep (1:100 dilution ...
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bioRxiv - Cell Biology 2019Quote: ... 20 ng/ml recombinant mouse macrophage colony-stimulating factor (Gibco, Burlington, ON), and penicillin/streptomycin antibiotics ...
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bioRxiv - Microbiology 2021Quote: ... 20 ng/ml of recombinant human epidermal growth factor (EGF, Life Technologies), human insulin (Sigma) ...
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bioRxiv - Developmental Biology 2021Quote: ... and 10 ng/ml bFGF recombinant human protein (Thermo Fisher Scientific, USA). From day 6 until day 12 the medium was changed once in two days with the addition of 1 mM valproic acid (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... The recombinant plasmid was linearized using the Ncol restriction enzyme (ThermoFisher Scientific) and the MEGAscript® T7 Kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and 20 U/ml recombinant interleukin-2 (IL-2; Thermo Fisher Scientific) at 37° C ...
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bioRxiv - Molecular Biology 2021Quote: ... The guide RNAs and recombinant Cas9-NLS protein (Thermo Fisher Scientific, B25642) were transfected into HEK293 T-Rex cells with Lipofectamine CRISPRMAX Cas9 Transfection Reagent (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: Recombinant baculovirus was generated using the Bac-to-Bac expression system (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The yield of the recombinant protein was estimated by Qubit 2.0 (Invitrogen) and the degree of purification by SDS-PAGE analysis.
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bioRxiv - Cell Biology 2020Quote: ... Human recombinant epidermal growth factor (hEGF) protein was purchased from Life Technologies and dissolved in PBS ...