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Citations for Thermo Fisher :
851 - 900 of 10000+ citations for 2' 3' Dimethyl 3 2 5 dimethylphenyl propiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Beads were gently washed 3 times with 1 ml of KPBS using a DynaMag-2 magnet (Thermo Fisher Scientific cat# 12321D). Beads were resuspended in 1 ml of KPBS and transferred to a new tube ...
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bioRxiv - Plant Biology 2023Quote: Qdot-labeled Cel7A was prepared by mixing 3 nM biotinylated Cel7A with 2 nM Qdot 655 (Thermo Scientific; catalog number: Q10123MP) in 50 mM sodium acetate ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptide mixtures were injected in 0.1% TFA into a C18 Acclaim PepMap100 trap-column (75 μm ID × 2 cm, 3 μm, 100Å, Thermo Fisher Scientific) for 3 minutes at 5 μL/min with 2% ACN ...
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bioRxiv - Neuroscience 2023Quote: ... the cell pellet was then resuspended in enzyme assay buffer again and sonicated at 3-second intervals for 2 minutes at 50% amplitude (Cat# FB120220, Fisher Scientific). The cell lysate was centrifuged at 1000 x g at 4°C for 10 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were typically passaged every 2-3 days by treating the cultures with 0.05% Trypsin-EDTA (Thermo Fisher Scientific, cat# 15400054) for 8-10 min at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Peptides were loaded first on a Trap column (Acclaim PepMap 100 3 µm C18 column of 2 cm length and 75 µm inner diameter, ThermoFisher Scientific) and then injected on an analytical column (EasySpray 15 cm column 2 µm 100 Å C18 column with 75 µm inner diameter ...
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bioRxiv - Biochemistry 2023Quote: ... High-resolution micrographs for single-particle reconstructions (Figure 2 and 3) were collected on a Titan Krios G4 (Thermo Fisher Scientific), which was operated at 300 kV acceleration voltage ...
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bioRxiv - Cell Biology 2022Quote: ... HCEnCs were rinsed in DPBS and passaged at a ratio of 1:2 or 1:3 with TrypLE (Thermo Fisher Scientific) for 10-15 min at 37°C in 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... The tumors were placed into a chilled conical tube containing 2-3 mL of TrypLE Express Enzyme (Gibco; cat. no. 1260413) and transported on ice to a biosafety hood ...
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bioRxiv - Developmental Biology 2022Quote: ... Table 2) incubated for 3 overnights at 4 °C in blocking buffer with Alexa-dye conjugated secondary antibodies (1:500, Molecular Probes) incubated for 1 overnight at 4 °C in blocking buffer ...
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Pan-tissue mitochondrial phenotyping reveals lower OXPHOS expression and function across tumor typesbioRxiv - Biochemistry 2023Quote: ... of 3 to 30% acetonitrile using a 2□μm EASY-Spray PepMap RSLC C18 75□μm × 250□mm column (Thermo Fisher) with a column temperature of 55□°C ...
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bioRxiv - Immunology 2023Quote: ... Serum starved PANC-1 and MiaPaCa-2 cells were incubated with human properdin (20 µg/ml) and CellEvent™ Caspase-3/7 Green Detection Reagent (Invitrogen) (5 μM ...
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bioRxiv - Microbiology 2023Quote: ... The products were enzymatically cleaned (3 ul of PCR reaction, 2 ul water, 1 ul of ExoSap-IT (Applied Biosystems 78200)) for 15 minutes at 37°C followed by 15 minutes at 80°C ...
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bioRxiv - Cell Biology 2023Quote: ... slides were rehydrated with 25mM HEPES (Sytox Green) or dPBS (Hoechst, TOTO-3) for 2 minutes followed by incubation in 1µM Sytox Green (Invitrogen, Waltham, MA) in 25mM HEPES ...
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bioRxiv - Neuroscience 2024Quote: ... cochleae were plated in six-well plates (2-3 explants/well) containing 1 ml media (growth medium DMEM (12430-054,Gibco Life Technologies) combined with 1% FBS (16000-044 ...
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bioRxiv - Biochemistry 2024Quote: ... which was equipped with a 75 μm x 2 cm trap column packed with C18 3 μm bulk resins (Acclaim PepMap 100, Thermo Scientific) and a 75 μm x 15 cm analytical column with C18 2 μm resins (Acclaim PepMap RSLC ...
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bioRxiv - Neuroscience 2024Quote: ... in 0.05 M TBS and then incubated in the dark for 2 h at room temperature with secondary antibodies: donkey anti-mouse Alexa Fluor 488 (3 µl/ml; Life Technologies) and donkey anti-rabbit Alexa Fluor 594 (5 µl/ml ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5 mM ATP and 1% fraction V BSA) and loaded with 3 μM Mag-Fura-2 AM (Thermo Fisher Scientific, #M1292) at room temperature for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... onto a nanotrap column (75 μm i.d. x 2 cm precolumn, packed with Acclaim PepMap100 C18, 3 μm, 100 Å; Thermo Fisher) using an EASY-nLC 1200 system (Thermo Fisher) ...
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bioRxiv - Genetics 2024Quote: 100,000 HEK293 cells in a 6-well plate were transfected with Antisense oligonucleotide harbouring 2’-O Me phosphorothioate modifications at each nucleotide position (Integrated technologies) using 3 ul of Lipofectamine 2000 (11668027, ThermoFisher Scientific) in one ml OPTIMEM I Reduced Serum Medium with no phenol red (11058021 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Libraries were aliquoted in 10 mL (OD600 ∼ 0.2) and re-grown for 2-3 h to extract plasmids using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) for sequencing and for subcloning into the expression strain ...
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... were transfected with wild-type human NOPR or NOPLight (0.8 μg DNA) and β-arrestin-2-mCherry (1 μg DNA) using 3 μL Lipofectamine 2000 (Thermo Fisher). After 24 h ...
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bioRxiv - Biophysics 2024Quote: ... The grids were blotted for 3 seconds at 100% humidity with force “2” and plunge frozen into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). 9496 Movies were recorded on a 300 keV Titan Krios microscope (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... 0.5 g of flash-frozen human prefrontal cortical post-mortem tissue was sliced with a razor blade into 2–3 mm3 sections in Hibernate-E media (Thermo Fisher) on ice ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Peptides were first loaded on the trap column (Acclaim PepMap 100 C18, 75 µm ID x 2 cm, 3 µm particle size, Thermo Scientific), washed with 0.1 % formic acid in water for 7 min at 5 µL/min and subsequently transferred to the separation column (IonOpticks Aurora C18 column ...
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bioRxiv - Microbiology 2024Quote: ... the cells were washed once with PBS and 3 mL of DMEM (2% FCS) containing 1% low melting point agarose (ThermoFisher Scientific) was added to each well ...
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bioRxiv - Neuroscience 2024Quote: ... DAPI was allowed to dry for approximately 3 minutes before immediately coverslipping with 1-2 drops of ProLong Gold (Invitrogen, P36930). Slides were protected from any surrounding light ...
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bioRxiv - Immunology 2024Quote: ... the reaction mixture was dialyzed against deionized water at 4 °C for 2 days using Slide-A-LyzerTM Dialysis Cassette (10K MWCO, 3 mL, Thermo Scientific) and stored at −70 °C.
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bioRxiv - Molecular Biology 2024Quote: ... Cells were routinely passaged every 2-3 days by washing twice with PBS lacking divalent cations and treating with StemPro Accutase (ThermoFisher A1110501).
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bioRxiv - Microbiology 2024Quote: ... 4 µl of peptide mixtures (1µg) were loaded onto an Acclaim PepMap precolumn (75 µm x 2 cm, 3 µm, 100 Å; Thermo Scientific) equilibrated in solvent A and separated at a constant flow rate of 250 nl/min on a PepMap RSLC C18 Easy-Spray column (75 µm x 50 cm ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were grown in cell culture dishes (Falcon) and passaged every 2-3 days via trypsinization with 0.0S% Trypsin-EDTA (2S300-0S4, Thermo Fisher Scientific) at 80-90% confluency ...
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bioRxiv - Cell Biology 2024Quote: ... were plated onto 6-well culture plates at 2×105 cells/well (concentration determined using the Countess 3 automated cell counter; Invitrogen: AMQAX2000) for downstream experiments (n=5 biological replicates/treatment) ...
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bioRxiv - Systems Biology 2024Quote: ... and incubated in total of 150 µL SH2 binding buffer containing 20 µM biotinylated SH2 domain on a rotator and 4 °C for 2-3 hours in low protein binding microcentrifuge tubes (1.5 mL, Thermo Scientific™). The functionalized magnetic beads are then washed twice in 1 mL of SH2 binding buffer ...
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bioRxiv - Biophysics 2024Quote: ... The grids were blotted for 3 seconds at 100% humidity with force “2” and plunge frozen into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). 7376 movies were recorded on a 300 keV Titan Krios microscope (Thermo Fisher ...
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bioRxiv - Biophysics 2024Quote: ... The grids were blotted for 3 seconds at 100% humidity with force “2” and plunge frozen into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). 41335 movies were recorded on a 300 keV Titan Krios microscope (Thermo Fisher ...
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bioRxiv - Bioengineering 2024Quote: Samples were washed 3 times with DPBS before being incubated with secondary antibodies and 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen, 10125092) at 1:500 in the blocking solution ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were grown to a confluency of 70-90% and passaged every 2-3 days via trypsinization with 0.05% Trypsin-EDTA (25300-054, Thermo Fisher Scientific). Cell lines were regularly checked for Mycoplasma contamination and confirmed negative ...
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bioRxiv - Cancer Biology 2024Quote: ... with an in-line Acclaim PepMap 100 C18 trap column (75 μm × 2 cm, 3 μm, 100 Å, Thermo Fisher Scientific) heated to 45 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptides were acidified to pH 2-3 using trifluroacetic acid (TFA) prior to LC-MS analysis on a Dionex U3000 UHPLC system (ThermoFisher Scientific) equipped with a column chromatography setup coupled to a Q Exactive Plus Orbitrap MS (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... Then 2 equivalents of fluorescein-5-maleimide (Invitrogen, #F150) was added and the mixture was incubated at room temperature for 20 min ...
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bioRxiv - Neuroscience 2022Quote: ... EdU (5-ethynyl-2’-deoxyuridine, ThermoFisher Scientific, cat# A10044) staining was performed by Click-it Alexa Fluor 647 imaging kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and 5 ng/ml IL-2 (Thermo Fisher Scientific), and then resuspended with 1 ml of virus per 6 x 106 cells ...
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bioRxiv - Cell Biology 2021Quote: 0.1mg of 5-ethynyl-2’-deoxyuridine (EdU) (Invitrogen, A10044) dissolved in PBS (50ul ...
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bioRxiv - Developmental Biology 2020Quote: 5-ethynyl-2’-deoxyuridine (EdU) (Invitrogen A10044, Carlsbad, CA) dissolved in PBS was administered to mice at indicated postnatal days ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 μM 5-ethynyl-2’-dexoyuridine (EdU, ThermoFisher Scientific) was added to the culture media for 2 hours ...
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bioRxiv - Developmental Biology 2021Quote: 5-ethynyl-2′-deoxyuridine (EdU) was purchased from ThermoFisher and stock solutions were created by dissolving the powder in dimethyl sulfoxide as instructed by the manufacturer ...
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bioRxiv - Cancer Biology 2021Quote: 5-ethynyl-2’-deoxyuridine (EdU, Molecular Probes, Life Technologies) was dissolved in DMSO to make a 10mM stock solution ...
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bioRxiv - Cancer Biology 2021Quote: 5-ethynyl-2’-deoxyuridine (EdU, Molecular Probes, Life Technologies) was dissolved in DMSO to make a 10mM stock solution ...
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bioRxiv - Genomics 2022Quote: EdU (5-ethynyl-2’-deoxyuridine) (Thermo Fisher Scientific, A10044) was added to culture media at 10 µM final concentration for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: ... 2 μg plasmid and 5 μl Lipofectamine 2000 (Invitrogen) were diluted into 200 μl Opti-MEM (Gibco ...