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Citations for Thermo Fisher :
801 - 850 of 10000+ citations for 2' 3' Dimethyl 3 2 5 dimethylphenyl propiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 3 μL (60 pmol) of this mixture was added to 2 μL of TrueCut™ Cas9 v2 (∼60 pmol, ThermoFisher A36498), incubated at room temperature for 15 minutes to promote formation of Cas9:gRNA complexes ...
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bioRxiv - Microbiology 2020Quote: ... The experimental setup was as follows: the peptide mixture was loaded onto a 75 μm × 2 cm precolumn (Acclaim PepMap 100, C18, 3 μm, Thermo Scientific) and eluted over 60 min using a 75 μm × 25 cm analytical column (Acclaim PepMap RSLC ...
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bioRxiv - Cell Biology 2020Quote: ... The peptides were applied to a C18 column (Acclaim PepMap 100 pre-column, C18, 3 μm, 2 cm × 75 μm Nanoviper, Thermo Scientific) and subsequently separated using an analytical column (EASY-Spray column ...
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bioRxiv - Cell Biology 2021Quote: ... were transiently transfected into 8 × 104 U2OS 2-6-3 cells in 4-well chamber slides using Lipofectamine 2000 reagent (Invitrogen, 11668019) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... Peptides were loaded onto an Acclaim PepMap 100 (75μm x 2 cm) C18 (3 μm, 100 Å) pre-column and separated on an EASY-Spray column (Thermo Scientific; ID 75μm x 50 cm ...
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bioRxiv - Cell Biology 2022Quote: ... 2 cm length) and EASY-Column (75 μm inner diameter, 10 cm length, 3 μm particle size, both Thermo Fisher Scientific). The separated peptides were analyzed using LTQ-Orbitrap Velos mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... and then 1 μg of each peptide sample was loaded on a rap column (75 µm × 2 cm, 3 µm, C18, Thermo Fisher). The eluate was loaded onto a reversed-phase analytical column (50 µm × 250 mm ...
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bioRxiv - Molecular Biology 2022Quote: ... The nTAP-MS peptides were applied on an Acclaim PepMap 100 C18 trap column (75 μm ID x 2 cm, 3 μm, Thermo Scientific) in 0.1% formic acid ...
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bioRxiv - Developmental Biology 2022Quote: ... 10μM EdU was added for 2h to either day 2 or day 3 cultures and developed using the Click-IT EdU Alexa Fluor imaging kit (Life Technologies).
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bioRxiv - Cell Biology 2022Quote: ... and loaded on a 20 mm pre-column (C18 Acclaim PepMapTM 100, 75 µm x 2 cm, 3 µm, 100Å, Thermo Scientific) and separated on a 50 mm analytical column (C18 Acclaim PepMapTM RSLC ...
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bioRxiv - Microbiology 2021Quote: ... recombinant SARS-CoV-2 Spike RBD protein (RBD, His Tag) was conjugated with EZ-LinkTM Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... Parasitemia was monitored daily by flow cytometry by diluting 10 μl of each parasite culture well from 2-3 biological replicates into 200μl of 1.0 μg/ml acridine orange (Invitrogen Life Technologies A3568) in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2020Quote: ... recombinant SARS-CoV-2 spike protein was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Immunology 2020Quote: ... tryptic peptides obtained from StageTip-based SCX fractionation were reconstituted in 0.1% formic acid and loaded on a nanoViper 2 cm (3 µm C18 Aq) trap column (Thermo Fisher Scientific). Peptide separation was carried out using EASY-Spray C18 analytical column (50 cm ...
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bioRxiv - Systems Biology 2022Quote: ... Peptide mixtures were injected in 0.1% TFA on a C18 Acclaim PepMap100 trap-column (75 μm ID x 2 cm, 3 μm, 100Å, Thermo Fisher Scientific) for 3 min at 5 μL/min with 2% ACN ...
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bioRxiv - Genetics 2022Quote: ... Samples were injected and concentrated on a trap column (PepMap100 C18, 3 μm, 100 Å, 75 μm i.d. x 2 cm, Thermo Scientific) equilibrated with 0.05% trifluoroacetic acid in water ...
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bioRxiv - Biochemistry 2020Quote: The gel was run for 2-3 cm and stained with colloidal coomassie dye G-250 (Gel Code Blue Stain Reagent, Thermo Scientific) after which each lane was cut out ...
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bioRxiv - Cell Biology 2020Quote: ... Peptides were first loaded onto a nano trap column (Acclaim PepMap100 C18, 3 μm, 100Å, 75 μm i.d. × 2 cm, Thermo Fisher Scientific), and then separated on a reversed-phase EASY-Spray analytical column (PepMap RSLC C18 ...
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bioRxiv - Plant Biology 2021Quote: ... qRT-PCR was performed with 2 × RealStar Green Power Mixture (gene star) in a QuantStudio 3 Real-Time PCR System (Applied Biosystems). Three biological replicates with three technical replicates were run for all genes ...
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bioRxiv - Plant Biology 2020Quote: ... 15N-labeled seedlings were then dried for 2-3 days at 65°C and further analyzed by Thermo Finnigan Delta plus XP IRMS (ThermoFisher Scientific). For primary root length determination ...
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bioRxiv - Systems Biology 2021Quote: ... coli phosphate-binding protein labeled with 7-Diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin (MDCC) (phosphate sensor, CAT # PV4406, Thermo Fisher) upon binding of the free phosphate GTP hydrolysis product (excitation at 425 nm ...
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bioRxiv - Microbiology 2020Quote: ... Samples were injected and concentrated on a trap column (PepMap100 C18, 3 µm, 100 Å, 75 µm i.d. x 2 cm, Thermo Scientific) equilibrated with 0.05% TFA ...
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bioRxiv - Physiology 2020Quote: ... Tissues were washed 2-3 times with PBS buffer to and were finally mounted with ProLong Diamond antifade mounting media (ThermoFisher Scientific) on a superfrost plus microscope slide (Fisherbrand ...
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bioRxiv - Biochemistry 2021Quote: ... 2 μL of each sample were loaded onto a RP-C18 column (15 mm × 75 μm × 3 μm, Thermo Fisher Scientific). 2% acetonitrile with 0.1 % formic acid (A ...
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bioRxiv - Immunology 2020Quote: ... D-PBS (D-PBS without Ca++ and Mg++) supplemented with 2% FBS and 3 mM cell culture grade EDTA (Life Technologies; Thermofisher) prior to monocyte isolation ...
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bioRxiv - Immunology 2021Quote: ... Peptides were loaded onto an Acclaim PepMap 100 (75µm x 2 cm) C18 (3 µm, 100 Å) pre-column and separated on an EASY-Spray column (Thermo Scientific; ID 75µm x 50 cm ...
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bioRxiv - Cancer Biology 2022Quote: ... cell density was adjusted to 106 cells/mL (and was never allowed to go over 3×106 cells/mL) and puromycin selection (2 μg/mL, Gibco, A1113803) was started ...
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bioRxiv - Biochemistry 2022Quote: ... Between 2 to 3 million cells were used per electroporation and were resuspended in 90 μl of buffer R (Thermo Fisher). Proteins were spun down at 16700 g for 10 minutes and then diluted with buffer R in a 1:1 ratio ...
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bioRxiv - Genetics 2022Quote: ... primary rat astrocytes were thawed into T75 flasks and fed once every 2-3 days with glial media {(MEM (Thermofisher 11095080) supplemented with 5% FBS (Gemini 900-108) ...
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bioRxiv - Microbiology 2022Quote: Proteins (8 μM final) were incubated with 0.16 mM of (2'-(or-3')-O-(trinitrophenyl) adenosine triphosphate (TNP-ATP) (Thermo Fisher Scientific), in 50 μl of Binding Buffer (10 mM HEPES-KOH (pH 8) ...
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bioRxiv - Microbiology 2022Quote: ... The grids were blotted with force 2 for 3 seconds and plunged into liquid ethane using a Vitrobot (FEI Thermo Fisher). Cryo-EM data were collected with a Titan Krios microscope (FEI ...
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bioRxiv - Molecular Biology 2022Quote: ... 3-4 mg total protein and 2 ug of KIF1C antibody or 10 ug mCherry antibody (Invitrogen mCherry Monoclonal Antibody (16D7)) were used ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptide mixtures were injected in 0.1% TFA on a C18 Acclaim PepMap100 trap-column (75 μm ID x 2 cm, 3 μm, 100Å, Thermo Fisher Scientific) for 3 min at 6 μL/min with 2% ACN ...
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bioRxiv - Molecular Biology 2022Quote: ... The grids were washed by dipping 2 times in PBS (37°C) before 3 μL of 1 μm Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: OE sections from mice injected with retrograde tracer were stained with Alexa Fluor 555- conjugated streptavidin to detect biotin-labeled dextran (1:500 in phosphate-buffered saline containing 3 % normal donkey serum and 0.3 % Triton-X100 for 2 h at room temperature, S32355, Thermo Fisher Scientific), or GAP43 (see above ...
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bioRxiv - Neuroscience 2024Quote: ... Control 1 (C1) and 2 (C2) were derived by StemBANCC from an unaffected volunteer and control 3 (C3) was purchased from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Fractionated samples and bead-enriched samples were individually loaded onto a C18 trap column (3 μm, 75μm × 2 cm, Thermo Fisher Scientific) connected in-line to a C18 analytical column (2 μm ...
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bioRxiv - Immunology 2024Quote: ... We electroporated 3 μg of ODNs or gDNA into 2 × 106 HEK293 and 293A cells by Neon Transfection System (Thermo Fisher) based on the manufacturer’s introductions.
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bioRxiv - Neuroscience 2024Quote: ... Each sample was loaded onto an Acclaim PepMap trap column (75 µm × 2 cm, 3 µm, C18, 100 Å, Thermo Scientific) and then separated on a nanoEase BEH C18 column (150 µm × 100 mm ...
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bioRxiv - Neuroscience 2024Quote: ... either for 2-3 cm or ran completely and stained with colloidal coomassie dye G-250 (Gel Code Blue Stain Reagent, Thermo Scientific). The lane was cut into bands ...
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bioRxiv - Molecular Biology 2024Quote: Protein concentrations from fractions 2 and 3 of fed human milk and digesta were determined using a Micro BCATM kit (Pierce, Thermo Fisher), following manufacturer instructions.
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bioRxiv - Biochemistry 2023Quote: ... cells were passaged every 2–3 days by washing twice with phosphate-buffered saline (PBS) and dissociation with 0.25% Trypsin (Life Technologies, 25200056).
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bioRxiv - Cancer Biology 2024Quote: For acute versus chronic proliferation measurements total cell number for acute and chronic was counted at day 0 and every 2-3 days until day 10 using Countess automated cell counter (Thermo Fisher). For Incucyte® proliferation assay plastic flat bottom 96 well plates were coated with 5 μg/mL human fibronectin in PBS for 1h in 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Total DNA was stained using 4’,6-diamidino-2- phenylindole (DAPI) diluted 1:10000 in PBS containing 3% BSA (Molecular Probes) to illuminate host cell nuclei ...
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bioRxiv - Microbiology 2023Quote: ... Samples were injected and concentrated on a trap column (PepMap100 C18, 3 μm, 100 Å, 75 μm i.d.×2 cm, Thermo Scientific) equilibrated with 0.05% trifluoroacetic acid in water ...
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bioRxiv - Cell Biology 2022Quote: ... Single-axis tilts were collected from −60° to +60° at 2° increments at 7 □µm defocus on a Falcon 3 camera (Thermo Fisher) operated in linear mode at 22,000X magnification ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were injected onto an Acclaim PepMap100 C18 trap column (75 μm i.d., 2 cm long, 3 μm, 100 Å, Thermo Scientific) and further separated on an Acclaim PepMap100 C18 analytical column (75 μm i.d. ...
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The effects of caloric restriction on adipose tissue and metabolic health are sex- and age-dependentbioRxiv - Physiology 2023Quote: ... Tissues in formalin were fixed at 4°C for 2-3 days before being washed and stored in DPBS (Life Technologies). Tissues on dry ice were stored at −80°C prior to downstream analysis.
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bioRxiv - Molecular Biology 2022Quote: ... C18, 3 μm, 100A), then separated using analytical column Acclaim PepMap RSLC (75um x25cm, nanoViper, C18, 2 μm, 100A) (Thermo Fisher). The flow rate was 300 nL/min ...
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bioRxiv - Microbiology 2023Quote: ... and applied to plasma-cleaned CF-300 2/1 grids before being blotted for 3 seconds in a Vitrobot Mark IV (Thermo Fisher) and plunge frozen into liquid ethane ...