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Citations for Thermo Fisher :
8751 - 8800 of 10000+ citations for 7 METHOXY 4 4 DIMETHYL 3 4 DIHYDRO 2H NAPHTHALEN 1 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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STAT3 protects HSCs from intrinsic interferon signaling and loss of long-term blood-forming activitybioRxiv - Immunology 2023Quote: ... mounted with 10 µL of ProLong Diamond with DAPI (4’,6-diamidino-2-phenylindole; Molecular Probes, Eugene, OR), and incubated for 24-48 hours prior to analysis ...
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bioRxiv - Neuroscience 2023Quote: ... samples were incubated for 48 h at 4°C in secondary antibodies: goat Alexa Fluor-conjugated antibodies (Invitrogen) directed against rat (Alexa Fluor 647 ...
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bioRxiv - Molecular Biology 2023Quote: ... 12 well or 15 well NuPAGE™ 4 to 12% Bis-Tris 1.5mm mini protein gel (Thermo Fisher) and run in 1x MES buffer (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... The sample was applied at 4 °C and 95% relative humidity inside a Vitrobot Mark IV (Thermo Scientific). The grids were then blotted for 7 seconds with +2 blot force and plunge-frozen in liquid ethane ...
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bioRxiv - Microbiology 2023Quote: ... and loaded onto a 10-well Novex NuPAGE 4% to 12% BT SDS-PAGE gel (Invitrogen, Carlsbad, CA). Electrophoresis was performed in MOPS running buffer at 150 V ...
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bioRxiv - Neuroscience 2023Quote: ... 10 uL of each sample was resolved by electrophoresis using a 4-12% NuPAGE (Thermo Fisher Scientific, #NP0335BOX). Semi-dry transfer was performed using the Trans-Blot Turbo System (Bio-Rad ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... The RNA isolation was followed by an additional DNase treatment using 4 units of TURBO DNase (Thermo Fisher) for 30 min at 37°C in 100 μl total volume ...
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bioRxiv - Bioengineering 2023Quote: ... 4 µg of the P2A-CD19Δ-mScarlet or CD19Δ-mScarlet construct was transfected using Lipofectamine 2000 (Life Technologies) as per manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... the iPSC-derived astrocytes were labelled using 20 μM Ca2+ indicator dye Fluo-4 AM (Invitrogen, ThermoFischer Scientific) for 30-60 minutes depending on absorption rate of individual cells until the basal fluorescence of fluo-4 in all cells plateaued to a constant level ...
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bioRxiv - Biophysics 2023Quote: ... Sample were rinsed in PBS and incubated with 4’,6-diamidino-2-phenylindole (DAPI) (Thermo Scientific™, USA) for 5 mins and Alexa 594-phalloidin (Invitrogen™ ...
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bioRxiv - Molecular Biology 2023Quote: ... Equal amounts of lysed extracts were loaded on a NuPAGE 4-12% Bis-Tris Gel (Invitrogen; Carlsbad, CA) and transferred to a polyvinylidene difluoride (PVDF ...
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MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: ... Samples were resolved in NuPAGE® 4-12% Bis-Tris gels using MES buffer (Invitrogen, Life Technologies, # NP0321) and transferred to a 0.45 µM nitrocellulose membrane (GE Healthcare) ...
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MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: ... Samples were resolved in NuPAGE® 4-12% Bis-Tris gels using MES buffer (Invitrogen, Life Technologies, # NP0321) and transferred to a 0.45 µM nitrocellulose membrane (GE Healthcare) ...
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bioRxiv - Cell Biology 2023Quote: ... were fixed with freshly prepared 4% formaldehyde in PBS solution (using 16% Methanol-free Formaldehyde; Thermo Fisher Scientific) for 20 min at RT ...
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bioRxiv - Genomics 2023Quote: ... The quality of gDNA was assessed through a measure of gDNA concentration with the Qubit 4 fluorometer (Invitrogen) and high molecular weight with the 4200 Tapestation (Agilent ...
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bioRxiv - Immunology 2023Quote: ... at 95°C for 5 min and separated on a 4-12% Bis-Tris gel (NuPAGE, Thermo Scientific) for 60 min at 170V ...
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bioRxiv - Genomics 2023Quote: ... The chromatin was incubated overnight at 4°C with 50 μl Protein A magnetic beads (Dynabeads, Thermo Fisher) pre-bound with 2.5 μl α-CENH3 (gift of Prof ...
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of proteins for each sample were separated on 4-12% Bolt Bis-Tris Plus gels (ThermoFisher) and then transferred to a PVDF membrane (0.45 µm pore size ...
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bioRxiv - Cell Biology 2023Quote: ... In brief, cells were fixed in 4% formaldehyde (Electron Microscopy Services, 15710) in RNase-free PBS (ThermoFisher, AM9625) for 20 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were then washed with cell staining buffer (Standard Biotools) before fixation in 4% paraformaldehye (Thermo Fisher Scientific). For measurement of intracellular cytokines ...
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bioRxiv - Cancer Biology 2023Quote: ... Monolayers of senescent and non-senescent MeT-5A cells were fixed using 4 % formaldehyde (24005; Thermo Fisher Scientific) at 4°C for 20 min ...
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bioRxiv - Genomics 2023Quote: ... Each reaction (100 μL total volume) contained 10 μg of genomic DNA (measured on Invitrogen Qubit 4 Fluorometer) and 1 μM of each primer and was run on the Bio-Rad C1000 thermal cycler with the following program ...
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bioRxiv - Microbiology 2023Quote: ... eluted fractions and what remains bound to the beads were analysed on 4-20% Tris-glycine gel (ThermoFisher) stained with Commassie blue.
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bioRxiv - Cancer Biology 2023Quote: Ten micrograms of protein was loaded onto a 4-12% Bis-Tris precast NuPage Gel (Thermo Fisher Scientific). Gel electrophoresis was performed at 140 V for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... and 5% 2-mercaptoethanol] and was resolved by SDS-PAGE on 4% to 12% Bis-Tris gels (Invitrogen). Proteins were transferred to a polyvinylidene difluoride immobilon TM-P membrane (Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... and 20µg loaded into each well of a NuPAGE 4-12% Bis-Tris Gel (Invitrogen, #NP0335BOX, Carlsbad, CA). Gels were transferred to a membrane using the iBlot2 system (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... DNA was quantified with the Qubit 4 Fluorometer and Qubit dsDNA HS assay kit (Invitrogen, Waltham, MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Grids were blotted for 4-6 seconds with blotforce of 15 on a Vitrobot Mark IV Vitrobot (ThermoFisher) and directly plunged into liquid-nitrogen-cooled liquid ethane.
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bioRxiv - Microbiology 2023Quote: ... at 65°C for 10 mins and resolved on a NuPAGE 4–12% Bis-Tris PAGE gel (Invitrogen) at 120 V for 1 h ...
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bioRxiv - Immunology 2023Quote: ... and 40 μg of each protein lysate were resolved on 4-12% Bis-Tris gels (Thermo Fisher, NP0322BOX) under denaturing conditions ...
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bioRxiv - Microbiology 2023Quote: ... 4 g/L dextrose, 5.957 g/L HEPES, 0.05 g/L hypoxanthine, 5 g/L Albumax II, Invitrogen) supplemented with 200 mM L-glutamine ...
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bioRxiv - Neuroscience 2023Quote: ... 35 µg of total protein was loaded onto a 4-12% (w/v) Bis-Tris polyacrylamide gel (Invitrogen) and electrophoresed before being electroblotted to a nitrocellulose membrane ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were seeded on 4-well Permanox Chamber Slides (Nunc™ Lab-Tek™ Chamber Slide System 177437) and imaged with an Olympus FV1000 confocal microscope as described previously (Liu et al. ...
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bioRxiv - Microbiology 2023Quote: ... the protein extracts were separated on a NativePAGE 4-16% Bis- Tris using NativePAGE 20X Running Buffer (Invitrogen) for the anode buffer ...
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bioRxiv - Immunology 2023Quote: ... the cells were washed twice with FACS buffer and fixed with 4% paraformaldehyde (PFA, Affymetrix, Santa Clara, CA). A total of ~200,000 lymphocyte-gated PBMCs (140,000 alive CD45+ ...
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bioRxiv - Immunology 2023Quote: ... cells were permeabilized for 20 minutes at 4°C in permeabilization buffer (#00-8333-56, Thermo Fisher Scientific), before staining for 30 minutes at 4°C in permeabilization buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Quantification and quality control of the isolated RNA was performed employing Qubit 4 fluorometer (Q33238, Thermo Fisher Scientific), Qubit RNA BR assay kit (Q10210 ...
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bioRxiv - Systems Biology 2023Quote: ... were loaded into the specified lanes of a Bolt 4-12% Bis-Tris Plus gel (Invitrogen™ #NW04125BOX) in Bolt™ MES SDS Running Buffer (Invitrogen™ #B0002) ...
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bioRxiv - Neuroscience 2023Quote: ... Briefly, cells grown on glass coverslips (Carolina Biological Supply, 633029) were fixed with 4% paraformaldehyde (Fisher Scientific, AAA1131336) for 15 min and permeabilized with 1% Triton X-100 (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... incubated for 5 min at 95°C and loaded onto a NuPAGE 4-12% Tris/Bis gel (ThermoFisher) with NuPAGE MOPS running buffer (ThermoFisher) ...
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bioRxiv - Immunology 2023Quote: ... Expression of GOI protein (Figure 2) was determined by SDS–PAGE using 4–12% gradient polyacrylamide gels (Invitrogen) and Coomassie staining and by Western blotting using antigen-specific sera.
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bioRxiv - Biochemistry 2023Quote: ... Degradation over time was visualized by running time points on a 4–12% Bis-Tris NuPAGE gel (Invitrogen) and staining with InstantBlue Coomassie Stain (Abcam).
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bioRxiv - Plant Biology 2023Quote: ... or using the Qubit DNA Broad Range kit and Qubit 4 Fluorometer (Thermo Fisher Scientific Inc, Waltham, MA). Quality was assessed with an Agilent 4200 TapeStation HS DNA1000 and Kapa Biosystems Illumina Library Quantification qPCR assays ...
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bioRxiv - Biochemistry 2023Quote: ... PhoA accumulation was analyzed by SDS-PAGE (NuPAGE™ 4 bis 12 %, Bis-Tris, Invitrogen, Waltham, MA, USA) and Western blot analyses were carried out using nitrocellulose membranes in the iBLOT2 system (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... The DNA-transfection reagent complex was prepared by combining 4 µg of pcDNA3.1_Mb4.WT (hGOAT) or mutant plasmid and 9 µL Lipofectamine 2000 transfection reagent (Invitrogen) in a total volume of 500 µL supplement free DMEM followed by incubation for 30 min at room temperature ...
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bioRxiv - Genetics 2023Quote: ... Extracts were analysed by SDS–polyacrylamide gel electrophoresis using 4-12% Bis-Tris NuPAGE protein gels (Life Technologies) and transferred onto nitrocellulose membrane in 2.5 mM Tris-base ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA concentration and purity were checked using the Qubit RNA BR Assay Kit on Qubit 4 Fluorometer (ThermoFisher) and NanoDrop ND-1000 (ThermoFisher) ...
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bioRxiv - Cell Biology 2022Quote: ... 5–10mg of protein per sample was run on a 4–12% NuPAGE Bis-Tris Gel (ThermoFisher Scientific) and then transferred to PVDF membrane by liquid transfer using NuPAGE Transfer buffer (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm, 4 μm, Thermo Fisher Scientific). The column temperature was held at 30 °C ...
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bioRxiv - Cell Biology 2022Quote: ... onto a Dionex IonPac AS11-HC column (2 mm × 250 mm, 4 μm particle size, Thermo Fisher Scientific) equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm ...