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Citations for Thermo Fisher :
8651 - 8700 of 10000+ citations for 7 METHOXY 4 4 DIMETHYL 3 4 DIHYDRO 2H NAPHTHALEN 1 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated using NuPAGE 4-12% precast Bis-Tris protein gels in MOPS SDS running buffer (Invitrogen), transferred onto Immobilon-P PVDF membranes (Millipore) ...
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bioRxiv - Cell Biology 2021Quote: ... 4 µg of the indicated R15 constructs were added to 800 µl of Opti-MEM media (Gibco, 11058) and mixed ...
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bioRxiv - Genetics 2020Quote: ... SDQ2357) incubated over-night rotating at 4°C together with 80µl magnetic protein A/G beads (Thermo Scientific). Samples were then washed twice in 150mM NaCl buffer ...
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bioRxiv - Neuroscience 2021Quote: ... incubated for 10 min in PBS/10 ug/mL 4’-6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) to stain nuclei ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amount of protein (micro-BCA method; Pierce, IL) were loaded on 4-12% gradient gels (Invitrogen, California), electrophoresed and transferred to PVDF membrane ...
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bioRxiv - Immunology 2022Quote: ... Samples were electrophoresed at 4°C for 30 min in Dark Blue cathode at 150V (Thermo Fisher, BN2007). After this initial electrophoresis ...
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bioRxiv - Neuroscience 2022Quote: ... 50 μm coronal sections of the striatum were incubated overnight at 4°C in CAS-block (Life Technologies) with a single primary antibody (see Table 1) ...
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bioRxiv - Neuroscience 2022Quote: Immunofluorescence was performed using glial fibrillary acidic protein (GFAP) and 4′,6-diamidino-2-phenylindole (DAPI) solution (ThermoFisher). GFAP primary and secondaries were diluted to a ratio of 1:1000 and 5:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... and electrophoresis was performed in NuPAGE 4 to 12% gradient Bis-Tris polyacrylamide protein gels (Thermo Fisher Scientific). Proteins were transferred to Immun-Blot PVDF membranes (Bio-Rad ...
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bioRxiv - Genetics 2022Quote: ... and ligation was performed in the ligation buffer for 4 hours at 16C (T4 DNA ligase; Life Technologies). After overnight decrosslinking at 65C ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were loaded onto a NuPAGE 4-12% Bis-Tris pre-cast SDS gradient gel (Thermo Fisher Scientific), transferred to a nitrocellulose membrane (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... the cell lysates were run onto NuPAGE®4–12% Bis-Tris Gels (Novex Life Technologies, Waltham, MA) and transferred onto PVDF membrane using the Bio-Rad transfer system ...
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bioRxiv - Bioengineering 2022Quote: ... the intracellular calcium flux within the hMOs was analyzed employing a Fluo-4 Calcium Imaging Kit (Thermo Fisher) according to the manufacturer’s instructions (n=4) ...
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bioRxiv - Bioengineering 2022Quote: ... hippocampi were washed in 4°C cold Hank’s Balanced Salt Solution (HBSS, #14175-053, Invitrogen, Waltham, MA, USA), and afterwards incubated for 1 h at 37 °C in a slowly rotated (~30 rpm ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions (200 μL) were incubated for 4 h in 8-well Nunc Lab-Tek chambers (Thermo Scientific #155361). Brightfield and Cy3 fluorescence images were captured on a Nikon Ti Eclipse wide-field microscope ...
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bioRxiv - Cancer Biology 2022Quote: ... TGF-β levels (pg/mL) were determined using a commercial murine ELISA kit (#BMA608-4; Invitrogen, Massachusetts, USA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Nuclei and F-actin were counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI, 300 nM, D1306, ThermoFisher) and phalloidin-AlexaFluor 647 (1:100 ...
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bioRxiv - Biochemistry 2022Quote: ... and incubated for 2 h at 4°C with 50 µl of IgG magnetic PanMouse Dynabeads (Thermo Fisher) equilibrated with IP buffer ...
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bioRxiv - Cell Biology 2022Quote: ... BD Biosciences)-coated wells (0.5 brains/4 cm2) and cultured in Dulbecco’s modified Eagle’s medium (DMEM) plus GlutaMAX (10564011; ThermoFisher Scientific), 20% fetal bovine serum (Hyclone) ...
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bioRxiv - Cell Biology 2022Quote: ... tissues were incubated in 30% sucrose overnight at 4°C and embedded in OCT (LAMB/OCT, Thermo Scientific). Sections of 12-µm thickness were cut on a cryotome (Cryostat Leica CM1950) ...
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bioRxiv - Cell Biology 2022Quote: ... The heated samples were briefly centrifuged at high speed before being resolved on 4-12% NuPAGE gel (Invitrogen). Proteins were transferred onto a PVDF membrane filter paper sandwich (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... 1.2% H2O2 in 0.5x SSC) and treated with proteinase K (4 μg/mL in PBSTx, Thermo Fisher 25530049) for 10 mins followed by fixation in 4% formaldehyde (10 mins) ...
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bioRxiv - Cell Biology 2022Quote: ... Calcium influx was visualized in N2a cells and HEK293 cells using Fluo-4 AM dye (Fisher Scientific, F14201) or the GCaMP6s HEK293 cell line described above ...
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bioRxiv - Cell Biology 2022Quote: ... Measurement of intracellular Ca2+ influx was conducted using the Fluo-4 DirectTM Ca2+ Assay Kit (Thermo Fisher Scientific). Fluorescence was measured using a SpectraMax Plus 384 microplate reader (excitation 495 nm ...
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bioRxiv - Cell Biology 2022Quote: Samples were either run on 4-12% NuPAGE or Bolt gels with the NuPAGE Bis-Tris system (Invitrogen) in MOPS SDS buffer or on 8% gels with the BIO-RAD system with SDS-running buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Protein gels were run according to standard procedure on a 4-12% Bolt Bis-Tris Plus gel (Invitrogen), blocked with Intercept Blocking Buffer (LI-COR) ...
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bioRxiv - Immunology 2022Quote: ... The cells were centrifuged at 400 g for 5 min at 4°C and resuspended in DPBS (GIBCO) supplemented with 5% FBS ...
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bioRxiv - Immunology 2022Quote: ... The slices were mounted with ProLong Diamond Antifade Mountant with 4’,6-diamino-2-phenylindole (DAPI) (ThermoFisher Scientific). Fluorescent images were analyzed with a Nikon confocal microscope (Nikon A1RMP ...
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bioRxiv - Microbiology 2022Quote: Equivalent amounts of total proteins were run in Bolt 4%-12% Bis-Tris gradient gels (Thermo Fisher Scientific) or 15% tris-glycine-SDS acrylamide gels and transferred to 0.2 μm-pore size nitrocellulose membranes in Towbin buffer (25 mM Tris ...
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bioRxiv - Microbiology 2022Quote: ... falciparum was maintained at 4% haematocrit in human RBCs in AlbumaxII media (RPMI-1640, 25 mM HEPES (Gibco), 367 μM hypoxanthine (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2022Quote: Drosophila melanogaster male S2 cells (subclone L2-4, Patrick Heun, Edinburgh) were cultured in Schneider’s Drosophila medium (Gibco) supplemented with 10% fetal calf serum (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were fixed with 4% paraformaldehyde and stained using the Click-IT Plus TUNEL Assay kit (Invitrogen, C10619) with Alexa Fluor 647 secondary per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The sections for cMAP3 experiments were then incubated overnight at 4°C with rabbit anti-GFP (Thermo Fisher Scientific Cat# A-11122 ...
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bioRxiv - Microbiology 2023Quote: ... Samples were transferred to 4°C for staining with anti-AF488-antibody (rabbit, Thermo Fisher Scientific; #A-11094) for 30 min on ice shaking ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... dishes were mounted with ProLong Diamond mounting medium containing 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Waltham, MA), and imaged using a Zeiss LSM 780 laser confocal microscope equipped with a C-Apochromat 40x/1.2W objective.
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bioRxiv - Neuroscience 2022Quote: ... Samples were boiled for 5 minutes then electrophoresed in pre-cast 4-12% NuPAGE Bis-Tris Gels (Invitrogen). Following transfer ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were then applied at 4ºC overnight in 1x PBS supplemented with Horse serum (4%, ThermoFisher, 16050114). The following primary antibodies were used ...
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bioRxiv - Neuroscience 2022Quote: Whole embryonic brains were dissected at E14.5 and E18.5 and then fixed in 4% (wt / vol) paraformaldehyde (28908, ThermoFisher) in PBS at 4 °C for 8 to 12 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Cleared lysates were incubated overnight at 4°C with 2μg rabbit anti-MATR3 antibody/sample (ThermoFisher A300-591A) conjugated to Dynabeads Protein G (ThermoFisher) ...
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bioRxiv - Neuroscience 2022Quote: ... Lysate and bead mix was incubated rotating overnight at 4°C with Baseline Zero DNAse (Fisher Scientific, #NC1424104). Following incubation ...
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bioRxiv - Neuroscience 2022Quote: ... respectively) pups were transcardially perfused with 4% paraformaldehyde (PFA) in 0.1M PBS (PBS tablets, 18912-014, Life technologies). Brains were post-fixed overnight at 4°C in 4% PFA in 0.1M PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... 20μg total protein were loaded on SDS-PAGE precast gradient gels (4–12% Nu-Page Bis-Tris, Invitrogen), followed by transfer to nitrocellulose membrane ...
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bioRxiv - Molecular Biology 2022Quote: ... collected in 50 μl fractions and analyzed by SDS-PAGE (NuPAGE™ 4 to 12%, Bis-Tris, Invitrogen) run in MES-SDS buffer at 190V for 50 minutes.
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bioRxiv - Microbiology 2022Quote: ... Supernatants from cell lysates were run on 4–12% NuPAGE Bis Tris precast gels (Life Technologies, Beverley, MA) using MES running buffer (Life Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibody was donkey anti-mouse Alexa 488 (4 μg/mL; Molecular Probes, catalog# A-21202, RRID: AB_141607). Slices were mounted on slides with Vectashield mounting medium containing DAPI (Vector Laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... 4 °C) and the concentration determined using a BCA assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific). After running an SDS PAGE (12%) ...
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bioRxiv - Immunology 2022Quote: ... Gelatin and collagen type 4 cleavage were examined using an EnzChek™ Gelatinase/Collagenase Assay Kit (ThermoFisher Scientific), with MMP-2 (AnaSpec ...
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bioRxiv - Molecular Biology 2022Quote: ... and media was replaced after 4-16 hr with media supplemented with 100 µg/mL Hygromycin B (Invitrogen) to start selection ...
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bioRxiv - Cell Biology 2022Quote: ... Neurons were then washed with 1x phosphate-buffered saline (PBS) once and fixed using 4% formaldehyde (Thermo Fisher)/4% sucrose (VWR ...
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bioRxiv - Physiology 2022Quote: ... Western blotting was performed with whole-cell lysates run on 4-12% Bis-Tris NuPage gels (Life Technologies) and transferred onto Immobilon-P Transfer Membranes (Millipore) ...