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Citations for Thermo Fisher :
8701 - 8750 of 10000+ citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The lysates were cleared by centrifugation at 18,000 x g and 4°C for 20 min and protein concentration was determined using the BCA Protein Assay Kit from Pierce™ (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... EVs from non-treated iRBCs and EVs from CQ-treated iRBCs were added containing 300 μg of protein which was calculated using a BCA assay kit (Thermo Fisher Scientific, USA). 1x PBS was used as the control without EVs ...
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bioRxiv - Genetics 2023Quote: ... and total protein concentration was estimated using Pierce Bicinchoninic Acid (BCA) protein assay kit according to the manufacturer’s protocol (cat. 23225, Thermo Fisher Scientific, IL, USA).
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bioRxiv - Evolutionary Biology 2023Quote: ... each IgG was quantified using the Pierce™ BCA Protein Assay Kit as per the manufacturer’s recommendations (Thermo Fisher Scientific, cat. no. 23225).
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bioRxiv - Physiology 2023Quote: ... the tissues were lyophilized to estimate the ratios of ng DNA per mg dry tissue in each group using the Quant-iT PicoGreen dsDNA assay kit (Invitrogen, Carlsbad, CA, USA) and a SpectraMax M Series Multi-Mode Microplate Reader (Molecular Devices ...
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bioRxiv - Evolutionary Biology 2023Quote: ATP levels were quantified in cell lysates using a luciferin/luciferase bioluminescence assay (ATP determination kit, Molecular Probes cat # A22066, Thermo Fisher Scientific) as previously described (Jara et al ...
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bioRxiv - Evolutionary Biology 2023Quote: ATP levels were quantified in cell lysates using a luciferin/luciferase bioluminescence assay (ATP determination kit, Molecular Probes cat # A22066, Thermo Fisher Scientific) as previously described (Jara et al ...
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bioRxiv - Biochemistry 2023Quote: α-synuclein was quantified in the striatum region of mouse brain utilizing the Enzyme-linked Immunosorbent Assay (ELISA) Kit from Invitrogen (Cat No. KHB0061) following the manufacturer‘s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... we quantified the cDNA concentration of a subset of samples using th eQuant-iT Picogreen dsDNA Assay kit (Thermo Fisher, Waltham, MA, USA). Standards ranging from 25pg/mL to 25ng/mL were prepared using the Lambda Standard DNA (100ug/mL ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA quality and concentration were estimated with NanoDrop One C spectrophotometer and Qubit 4MT with a dsDNA HS Assay Kit (Invitrogen, Carlsbad, CA, USA). Total of 83 Lonsdalea quercina strains (one strain per tree ...
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bioRxiv - Bioengineering 2023Quote: ... the supernatants were transferred to clean tubes and their protein concentrations were measured using a BCA Protein Assay Kit (Pierce Thermo Scientific, MA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... Germany) and quantified with a Qubit 4 Fluorometer using the Qubit RNA High Sensitivity (HS) assay kit (Thermo Fisher Scientific, Waltham, MA, USA). cDNA libraries for each sample were constructed from 100 ng of RNA using the PrimeScript RT master mix (Takara Bio ...
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bioRxiv - Microbiology 2024Quote: ... The concentration of extracted DNA was determined using a Quant-iT™ PicoGreen® dsDNA Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). Amplicon libraries were generated using the Illumina® 16S metagenomic sequencing library preparation protocol ...
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bioRxiv - Plant Biology 2024Quote: ... while its concentration was estimated using a fluorescent DNA intercalating agent (e.g., Quant-iT PicoGreen dsDNA Assay Kit, Thermo Fisher Cat. No. P7589) and a microplate reader (Thermo Fisher Scientific)) ...
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bioRxiv - Genomics 2024Quote: ... the sample was quantified by the Qubit® Fluorometer and Qubit™ dsDNA HS and BR Assay Kits (Invitrogen™ Cat. No. Q32851). Overnight pulse-field gel electrophoresis was used to examine the molecular weight of the isolated DNA ...
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bioRxiv - Developmental Biology 2024Quote: ... Gdf9-Cre were cultured in KSOM medium supplemented with 50 μM HPG from Click-iT® HPG Alexa Fluor Protein Synthesis Assay kits (Thermo Fisher Scientific) for 2 h ...
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bioRxiv - Cell Biology 2024Quote: ... Apoptotic assays: Apoptotic cells were identified by labeling 3’ ends of DNA using a BrdU apoptosis kit (Fisher Scientific cat. 88-6671-88) and anti-BRDU antibodies ...
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bioRxiv - Physiology 2024Quote: ... and protein content of the remaining lysate was measured for normalisation of results (BCA Protein Assay Kit; #23225, Thermo Fisher Scientific, Rockford, IL).
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bioRxiv - Microbiology 2023Quote: ... PCR amplicons were purified with Vazyme VAHTSTM DNA Clean Beads (Vazyme, Nanjing, China) and quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Carlsbad, CA, USA). After the individual quantification step ...
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bioRxiv - Microbiology 2023Quote: ... Amplicons were purified using the Genomic DNA Clean & Concentrator®-10 (D4011) and quantified using the broad-range double-stranded DNA assay kit (Invitrogen, USA, Q32853) on the Qubit 2.0 Fluorometer (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... PRP pellets were lysed in lysis buffer and their protein concentration was measured using Pierce BCA protein assay kit (Thermo Scientific, Cat. 23225) and a Tecan Infinite 200 plate reader (Tecan Group Ltd. ...
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bioRxiv - Cancer Biology 2023Quote: ... Supernatant was collected and protein concentration was determined using the PierceTM BCA protein assay kit following manufacturer’s instructions (Thermo Fisher Scientific, Massachusetts, USA). Each sample was separated by non-reducing SDS-PAGE (6% acrylamide gel ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatant was transferred to a new tube and the protein concentration was measured by the BCA assay kit (Thermo Fisher Scientific, USA).
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bioRxiv - Microbiology 2023Quote: ... The quantity and quality of the purified total RNA were assessed using a Thermo Fisher Scientific Qubit 4.0 fluorometer with the Qubit RNA BR Assay Kit (Thermo Fisher Scientific, Waltham, USA) and an Advanced Analytical Fragment Analyzer System using a Fragment Analyzer RNA Kit (Agilent ...
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bioRxiv - Microbiology 2023Quote: ... cell culture supernatants were collected and Gluc activity was quantified according to the manufacturer protocol using Pierce™ Gaussia Luciferase Glow Assay Kit (16160, Thermo Fisher Scientific) and GloMax plate reader (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... which was quantified using both optical density at 600 nm (OD600) and total protein content estimated using a BCA protein assay kit (Thermo Scientific, MA, USA). For quantification of the expression of the arsM gene ...
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bioRxiv - Physiology 2023Quote: ... The purity of RNA was examined with the NanoPhotometer® spectrophotometer (IMPLEN) and quantity was determined by Qubit® RNA Assay Kit in Qubit®2.0 Flurometer (Life Technologies). RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies ...
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bioRxiv - Genomics 2023Quote: ... We quantified the adapter-ligated and barcoded DNA using a Qubit fluorimeter (Qubit 1X dsDNA High Sensitivity Assay Kit, Life Technologies, Carlsbad, CA).
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bioRxiv - Cell Biology 2023Quote: ... Total protein was determined by the bicinchoninic acid method according to the manufacturer’s instructions (BCA Protein Assay Kit, Pierce-Thermo Fisher Scientific, Melbourne, Australia).
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bioRxiv - Microbiology 2023Quote: ... cell culture supernatants were collected and Gluc activity was quantified according to the manufacturer protocol using Pierce™ Gaussia Luciferase Glow Assay Kit (16160, Thermo Fisher Scientific) and GloMax plate reader (Promega) ...
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bioRxiv - Immunology 2023Quote: ... The quantity of the RNA was assessed prior to the experiments according to the guidelines of the Qubit RNA high sensitivity (HS) assay kit and Qubit Fluorometer(Thermo Fisher Scientific, USA).
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bioRxiv - Microbiology 2023Quote: ... Final RNA concentrations were measured using the Qubit® 2.0 Fluorometer with RNA High Sensitivity (HS) Assay Kit (Life Technologies Corporation, Carlsbad, CA) at the completion of the purification ...
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Impaired yolk sac NAD metabolism disrupts murine embryogenesis with relevance to human birth defectsbioRxiv - Developmental Biology 2024Quote: ... Insoluble proteins and other debris were removed by centrifugation at 12000 rpm for 7 minutes in a microcentrifuge and protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific, Rockford, USA) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2024Quote: ... After incubation cells were harvested and fixed and the labelling of EdU incorporation was conducted by Click-iT™ EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit (Molecular Probes). EdU-Alexa Fluo 488 positive cells were detected by flow cytometry on Quanteon analyzer and data analysis was performed using FlowJo_V10.8.1.
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bioRxiv - Genomics 2024Quote: ... DNA quantity and quality were assessed using the Nanodrop 2000 spectrometer and Qubit 4 fluorometer with the dsDNA BR assay kit (Invitrogen, Carlsbad, CA, USA), and integrity was confirmed by gel electrophoresis ...
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bioRxiv - Bioengineering 2024Quote: To measure the mOx40L protein abundance in EVs the Mouse TNFSF4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) kit (Invitrogen, Thermo Fisher Scientific) was used according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Purity and concentration of the recombinant proteins was assessed by SDS-PAGE and the Qubit Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA). Gel filtration was used to recover the recombinant protein fraction according to its expected size on an AKTA Ettan LC FPLC System (Amersham Pharmacy Biotech ...
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bioRxiv - Cell Biology 2024Quote: ... The concentration of bEV was measured with Qubit™ Protein and Protein Broad Range (BR) Assay Kits (Thermo Fisher Scientific, Waltham, MA, USA). The expression level of EV markers including CD9 ...
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bioRxiv - Cell Biology 2024Quote: ... the measured concentrations of various lipid classes (nmol/μL) were divided by protein concentration (μg/μL) that was assessed using BCA Protein Assay Kit (Thermo Fisher Scientific, 23225).
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bioRxiv - Molecular Biology 2024Quote: ... using Click-iT™ Plus TUNEL Assay Kits for In Situ Apoptosis Detection with Alexa Fluor™ 594 dye (Invitrogen, Catalog number: C10618), as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The gradient fraction at the interface between the 23% and 10% Percoll containing synaptosomes were collected and protein concentration determined by Pierce Bradford Protein Assay Kit (Thermo Fisher Scientific, 23200).
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bioRxiv - Neuroscience 2024Quote: ... Raw cytokine values per well were normalized over nuclei number per well based on fluorescent staining of the fixed iAstrocytes in the plate or over protein content per well determined by Pierce™ BCA protein assay kit (Thermo Scientific, 23225). Pierce™ BCA protein assay was performed in a microplate as described in the user guide provided by Thermo Scientific.
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bioRxiv - Biochemistry 2024Quote: ... centrifuged at 16,000 x g for 15 minutes at 4°C and protein content determined by Pierce BCA protein assay kit (Thermo Fisher Scientific 23225). 25µg total lysate was removed as input and 0.5mg total protein was incubated with pre-washed magnetic M2 anti-FLAG (Sigma-Aldrich M8823-5ML ...
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bioRxiv - Immunology 2024Quote: ... The acid-extracted histones were collected in the supernatant fraction after centrifugation at 650g for 10 min at 4 °C and were neutralized with 2 M NaOH at 1/10 the volume of the supernatant before quantifying protein concentration using BCA® Protein Assay kit (Thermo Scientific #23225)139 ...
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bioRxiv - Genomics 2024Quote: ... Concentration and final size distribution of the library were evaluated using a Qubit 1X dsDNA HS Assay Kit (Thermo Fisher, Waltham, MA, USA) and Femto Pulse System (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... The concentration of each DNA sample was determined using a high sensitivity Quant-iT™ dsDNA Assay Kit and a Qubit Fluorometer (Thermo Fisher Scientific). Purified ChIP DNA (10 µg ...
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ATP-dependent citrate lyase Drives Left Ventricular Dysfunction by Metabolic Remodeling of the HeartbioRxiv - Molecular Biology 2024Quote: ... RNA concentration was determined using a Qubit 4.0 fluorometer and Qubit™ RNA HS Assay Kit (Cat#Q32852, Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Extracted DNA was quantified in a Qubit 4 fluorometer using the dsDNA HS and the dsDNA BR assay kits (Thermo Scientific, MA, USA). Additional quality evaluation included NanoDrop 2000 spectrophotometer (Thermo Scientific ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The protein concentration was determined by the BCA (bicinchoninic acid assay) assay (Thermo Fisher Scientific, Waltham, MA, USA) and homogenized at 0.1 mg ml −1 ...
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bioRxiv - Genomics 2019Quote: ... cDNA was diluted 1 in 4 with HLA-DQB1 (Assay ID:Hs00409790) and actinB (Assay ID:Hs01060665) probes and Taqman MasterMix (Thermofisher). Samples were run on an ARIAmx qPCR machine (Agilent ...