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Citations for Thermo Fisher :
8551 - 8600 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Cells of each cell line with ATRXKD or ATRXCT were seeded (5000 cells/well) in 0.35 % agarose cell medium (16500-500, Invitrogen, Carlsbad, CA, USA) onto a 6-well plate containing a 0.5 % agar base ...
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bioRxiv - Cell Biology 2020Quote: ... ES cells and T24 cells were fixed after 24h and T48 cells were fixed after 48h using 4% PFA (Fisher Scientific, #11586711) for 10 minutes at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... For analysis of immune cell recruitment into the peritoneal cavity the collected peritoneal cell suspension was centrifuged and red blood cells in the cell pellet were lysed with 1 mL ACK (Thermo Fisher Scientifics). After washing the cell suspension was counted using Neubauer Counting chamber and 1×106 cells were used for flow cytometry staining.
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bioRxiv - Cell Biology 2022Quote: ... Cells were plated at a cell density of 5×103 cells/cm2 in 24-well plates with MEM-A (GIBCO, 12561-056) supplemented with 10% (vol/vol ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cells were cultivated at 1.5×106 cells/ml in CST™ OpTimizer T cell expansion SFM medium (Thermo Fisher, A1048501) supplemented with 20 U/ml of recombinant huIL-2 (Roche ...
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bioRxiv - Immunology 2020Quote: Drug treated or siRNA transfected HBEC 5637 cells were collected from 6-well plates (containing 300,000 cells/well) using cell dissociation buffer (Life Technologies, Waltham, MA). The cells were lysed with RIPA lysis buffer supplemented with Halt™ Protease and Phosphatase Inhibitor Cocktail (Thermofisher ...
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bioRxiv - Immunology 2020Quote: ... Stimulated PBMCs and BAL cells were stained for dead cells using the LIVE/DEAD Fixable Near-IR Dead Cell Stain (Life Technologies, OR) and then surface-stained with the following antibodies ...
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bioRxiv - Immunology 2020Quote: Isolated splenic B cells (~10–20×106 cells) were stained first with 5 μL (5mM) Cell Trace Violet (C34557, Thermo Fisher Scientific) in 10 mL of PBS for 10 min at RT and let to recover in complete RPMI (+37°C ...
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bioRxiv - Immunology 2022Quote: ... cell supernatants were collected for cytokine/chemokine analyses and cells were stained with Fixable Aqua Dead Cell Stain Kit (Invitrogen/Thermo Scientific) for determination of cell surface expression of co-stimulatory molecules by FACS (FACSCanto II ...
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bioRxiv - Microbiology 2022Quote: ... RAW264.7 cells passaging was achieved by gentle cell scraping and seeding cells at a density of 3×106 cells/T75 flask (Nunc; Thermo Fisher Scientific).
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bioRxiv - Pathology 2019Quote: ... Red blood cells in the cells pellet were removed by lysis with cold water and resultant cells enumerated on a Countess Automated Cell Counter (Invitrogen, Carlsbad, CA) then stained for flow cytometry.
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bioRxiv - Genomics 2020Quote: ... cells were dissociated using Accutase and cells were stained with LIVE/DEAD™ Fixable Far Red Dead Cell Stain Kit (Thermofisher, #L34973). The cells were washed with PBS ...
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bioRxiv - Immunology 2020Quote: ... BSC-1 cells were seeded at 1 x 105 cells per well in a sterile 12-well cell culture plate (Nunc, NY, USA) a day before the assay ...
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bioRxiv - Genomics 2019Quote: ... using a Sony SH800 cell sorter and a live/dead cell stain (LIVE/DEAD Fixable Green Cell Stain Kit, for 488nm Excitation, Thermo Fisher – L34969). Dilutions were made from all 14 samples as outlined in the Chromium Single Cell 3’ Reagent Kit v2 User Guide (10X Genomics ...
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bioRxiv - Immunology 2021Quote: ... T2 target cells and Epstein-Barr Virus-transformed B lymphoblastoid cell lines (EBV-LCLs) cells were cultured in RPMI-1640 (Thermo Fisher Scientific) supplemented with 10% FCS and 1% penicillin/streptomycin (#P433 ...
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bioRxiv - Cell Biology 2022Quote: ... cell counting was performed with trypan blue staining to assess cell viability (>90%) using an automated cell counter (CountessTM, Thermo Fisher Scientific). Conditioned medium was then collected and centrifuged for 10 min at 300 g and 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... Cell suspensions were filtered using a 40-μm nylon cell strainer and red blood cells were removed by red blood cell lysis solution (Thermo Fisher Scientific). Dissociated cells were washed with DPBS containing 2% FBS ...
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bioRxiv - Cell Biology 2024Quote: Unguided differentiation25 of iPS cells to neural progenitor cells (NPCs) was started by subculturing the iPSCs single cell using Tryple Express Enzyme (Life technologies, 12605010) at a density of 10e4 cells/cm² in mTesR1 medium (Stemcell Technologies ...
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bioRxiv - Immunology 2024Quote: Cell viability was measured by staining cells with LIVE/DEAD Fixable Aqua Dead Cell Stain or Blue Dead Cell Stain (Thermo Fisher Scientific) for 20 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: LN-229 cells were seeded at 2×10^5 cells/well into in 6-well Nunc™ Cell-Culture Treated Multidishes (ThermoFisher, #140675) and incubated overnight in reduced serum media at 37°C ...
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bioRxiv - Immunology 2022Quote: Wild-type C57BL/6 splenic-T cells are isolated using Stemcell EasySep™ Mouse T-cell Isolation kit (Stemcell #19851) and stained with Cell Trace Violet (ThermoFisher #C34571). These cells are then stimulated with anti-CD3/CD28 beads (Gibco #11456D ...
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bioRxiv - Cancer Biology 2023Quote: ... we changed medium to LG/LF medium without added estrogen and cultured cells overnight before harvesting cells with cell dissociation buffer (Gibco, ThermoFisher Scientific). We resuspended cells at 1 x 105 cells per 100 µl sterile 0.9% NaCl for intracardiac injection ...
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bioRxiv - Biochemistry 2023Quote: ... Virions were transduced into BT549 breast carcinoma cells and stably expressing cell lines were produced by growing the cells in RPMI 1640 (Thermo Fisher #61870044) + 5 % FBS (Gibco #10500064 ...
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bioRxiv - Microbiology 2023Quote: ... RAW264.7 cells passaging was achieved by gentle cell scraping and seeding cells at a density of 3×106 cells/T75 flask (Nunc; Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... The isolated cells were stained for cell viability using a Live/Dead Blue cell stain (L34962, Thermo Fisher Scientific, Waltham, MA, USA) and blocked with 4% bovine serum albumin (BSA) ...
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bioRxiv - Biophysics 2023Quote: In vitro cytotoxicity of the complexes was evaluated using U-2 OS cells and an LDH assay kit.42 Cells were counted with a Countess II Automated Cell Counter (ThermoFisher Scientific, USA) and seeded in 96-well plates at a density of 10,000 cells per well in 100 μL of full DMEM ...
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bioRxiv - Developmental Biology 2024Quote: ... HBVP cells were seeded at an initial concentration of 1.3X104 cells/cm2 in T-175 cell culture flasks (ThermoFisher Scientific, Cat.#: 178883) pre-coated with gelatin (4mg/mL in 1X PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... and respective control cells of CAL27 and FaDu head and neck squamous cell carcinoma cell lines were fluorescently dyed using CMFDA (Thermo Scientific, # C2925) and plated at a density of 10,000 cells per well in a 96-well plate ...
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bioRxiv - Cancer Biology 2021Quote: ... and large cell clumps were removed by passing the cell solution through sterile 40 μm nylon mesh cell strainers (Fisher Scientific, Hampton, NH). Subsequently ...
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bioRxiv - Molecular Biology 2020Quote: ... The transfection complex and BxPC3 cells and PNAC-1 cells were then mixed together and seeded in 6-well (2 x 105 cells/well) cell culture plates (Nunc, Rochester, NY, USA) and cultured at 37°C for 24 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Bert Volgenstein at the Core Cell center Baltimore) cells and Hela cells were maintained in RPMI 1640 (Gibco; ref 61870-010; lot 1880320) supplemented with 10% FBS (Gibco ...
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bioRxiv - Microbiology 2021Quote: MT-2 and MT-4 cells (Japanese Collection of Research Bioresources Cell Bank; JCRB Cell Bank, Japan) were cultured in RPMI1640 medium (Gibco, Thermo Fisher Scientific, USA) with Fetal Bovine Serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... the TZM-bl cells (104 cells/well) in complete HiDMEM medium and PBMCs (105 cells/well) in complete RPMI (Thermo Scientific; OK, USA) supplemented with 10% FBS ...
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bioRxiv - Immunology 2021Quote: ... Live/dead cells were discriminated by staining cells with the LIVE/DEAD® Fixable Near-IR Dead Cell Stain kit (L10119, Thermo Fisher Scientific) (dilution 1:500) ...
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bioRxiv - Microbiology 2021Quote: ... the cells were transferred to a 24-well plate (5 × 105 cells/500 µL cell medium/well; Thermo Fisher Scientific, Waltham, MA, USA) overnight ...
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bioRxiv - Microbiology 2022Quote: ... RAW264.7 cells passaging was achieved by gentle cell scraping and seeding cells at a density of 3×106 cells/T75 flask (Nunc; Thermo Fisher Scientific).
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bioRxiv - Pathology 2023Quote: ... and the number of 4T1 cells was counted using an automated cell counter (Countess™ 3 FL Automated Cell Counter, Thermo Fisher Scientific). A cell resuspension with a final cell density of 1 × 106 cells/ml was prepared in PBS.
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bioRxiv - Cancer Biology 2023Quote: Cell dead and apoptosis were determined in PB3 and MDA-MB-231 cells using the Dead Cell Apoptosis Kit (Thermo Fisher Scientific #V35114) following different treatments according to the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2023Quote: ... processed to yield a single cell suspension and EpCAM (CD326) positive cells were isolated by magnetic cell sorting (Magnisort®, Thermo Fisher Scientific) using anti-CD326-biotin (13– 5791-82 ...
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bioRxiv - Microbiology 2023Quote: ... RAW264.7 cells passaging was achieved by gentle cell scraping and seeding cells at a density of 3×106 cells/T75 flask (Nunc; Thermo Fisher Scientific).
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bioRxiv - Biophysics 2021Quote: ... coverslips with stained cells were mounted in an Attofluor cell chamber (ThermoFisher Scientific A7816) with freshly prepared STORM imaging buffer (see composition below ...
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bioRxiv - Biophysics 2021Quote: ... The differential cell counts were performed on cells cytocentrifuged onto glass slides (Fisher Scientific) stained with the Hema 3 Staining System (Fisher Diagnostics ...
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bioRxiv - Cell Biology 2020Quote: ... mononucleated cells were collected using 70 µm cell strainer (Thermo Fisher Scientific, Waltham, MA). To facilitate rapid isolation of a pure pharyngeal and hind limb SCs ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed and stained with SytoxTM Green Dead Cell Stain (Thermo Fisher) before running on flow cytometry.
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bioRxiv - Immunology 2021Quote: ... The cells were then washed and the SytoxTM green dead cell stain (Thermo Fisher) was added for cell viability ...
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bioRxiv - Immunology 2021Quote: ... Bulk unstained cells from the dLN suspended at 4x106 cells/mL in DMEM (Gibco) with FBS (5% vol/vol ...
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bioRxiv - Developmental Biology 2021Quote: Cells were detached from cell culture plates by incubation with 0.25% trypsin-EDTA (Invitrogen) plus 2% chick serum (Sigma ...
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bioRxiv - Immunology 2020Quote: CTV labelling of cells was performed using the CellTrace Violet Cell Proliferation Kit (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... target cells were stained with a CellTrace™ Violet Cell Proliferation Kit (Life Technologies) and primary NK cells ex vivo were stimulated with IL-2 in culture for six to eight days ...
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bioRxiv - Cancer Biology 2022Quote: ... About one million dissociated cells were counted with an automatic cell counter (ThermoFisher Scientific). 3D-dECMs derived from normal peritoneal tissue and PM were incubated overnight at 37 °C in DMEM-F12 supplemented with 10% FBS (Euroclone ...