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Citations for Thermo Fisher :
8701 - 8750 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... samples were resuspended in binding buffer containing a 1:100 dilution of mouse anti-human-IgG1-Fc conjugated to Alexa Fluor 488 (Thermo Fisher Scientific, Rockford, IL; A-10631) and incubated for 30 minutes at room temperature in the dark ...
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bioRxiv - Molecular Biology 2023Quote: ... Evaluation of proliferation in tissues using antibodies against Ki67 or BrdU (anti-human Ki67 and anti- BrdU monoclonal antibodies; Thermo-Fisher Scientific and BD BioSciences, USA, respectively) was performed as previously described {GarciaMelendrez:GKcY05su } ...
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bioRxiv - Neuroscience 2022Quote: ... were incubated with 100 ng/μL of mouse anti-human CD171 (L1CAM, neural adhesion protein) biotinylated antibody (clone 5G3, eBioscience/Thermo Fisher Scientific; Catalog # 13-1719-82); mouse anti-human GLAST (ACSA-1 ...
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bioRxiv - Bioengineering 2024Quote: Engineered cardiac tissues and hiPSC-cardiomyocytes cultured with patient IgG were fixed and incubated with secondary anti-human IgG (Thermo Fisher Scientific, cat. no. A-11013) to visualize cellular autoantibody targets ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary anti-human CD38 antibody was applied at a dilution of 1:250 and incubated overnight at 4°C (Thermo Scientific clone RM388 Cat# MA5-36061). After washing with PBS ...
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bioRxiv - Cell Biology 2023Quote: ... HUVECs were grown to ∼80% confluence followed by transfection with 100 nM human ERG Silencer Select (ID #s4811) or nontargeting siRNA oligos (Life Technologies: ERG #4392420 and nontargeting #AM4635) using lipofectamine RNAiMAX (Life Technologies ...
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bioRxiv - Immunology 2023Quote: ... recombinant human IL-3 (R&D, Cat. 203-IL-050/CF, 25 ng mL-1) or c) human M-CSF recombinant protein (Invitrogen, Cat. PHC9501, 100 ng mL-1). A partial medium change was performed every 48 hours with 2x cytokine composites to replenish cytokines ...
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bioRxiv - Molecular Biology 2024Quote: Human fibroblasts were seeded in a 24-well plate and stimulated with tumor necrosis factor-α (TNF-α) (5 ng/ml; Life Technologies, Landsmeer, the Netherlands) plus lymphotoxin α1β2 (50 ng/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were transduced with lentiviruses expressing Sg RNA with no sequence homology to any region of the human genome (LentiArray™ CRISPR Negative Control Lentivirus, Thermo Fisher Scientific, Cat No# A32327). Vehicle-treated knockouts were normalized to vehicle-treated negative control Sg RNA whereas ...
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bioRxiv - Molecular Biology 2020Quote: ... the media was replaced with a 1:10 ratio of regular cell culture media to Live Cell Imaging Media (Live Cell Imaging Solution (Life Technologies # A14291DJ) supplemented with 10% FBS ...
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bioRxiv - Biophysics 2020Quote: ... cells were detached from cell culture dishes using a trypsin-free chelator-based cell dissociation reagent (Versene, Thermo Fisher Scientific, Waltham, MA). The cells were seeded on samples in the same media as above ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were quantified using trypan blue staining followed by automated cell counting using the Countess® Automated Cell Counter (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2021Quote: Cells of each cell line with ATRXKD or ATRXCT were seeded (5000 cells/well) in 0.35 % agarose cell medium (16500-500, Invitrogen, Carlsbad, CA, USA) onto a 6-well plate containing a 0.5 % agar base ...
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bioRxiv - Cell Biology 2020Quote: ... ES cells and T24 cells were fixed after 24h and T48 cells were fixed after 48h using 4% PFA (Fisher Scientific, #11586711) for 10 minutes at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... For analysis of immune cell recruitment into the peritoneal cavity the collected peritoneal cell suspension was centrifuged and red blood cells in the cell pellet were lysed with 1 mL ACK (Thermo Fisher Scientifics). After washing the cell suspension was counted using Neubauer Counting chamber and 1×106 cells were used for flow cytometry staining.
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bioRxiv - Cell Biology 2022Quote: ... Cells were plated at a cell density of 5×103 cells/cm2 in 24-well plates with MEM-A (GIBCO, 12561-056) supplemented with 10% (vol/vol ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cells were cultivated at 1.5×106 cells/ml in CST™ OpTimizer T cell expansion SFM medium (Thermo Fisher, A1048501) supplemented with 20 U/ml of recombinant huIL-2 (Roche ...
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bioRxiv - Immunology 2020Quote: Drug treated or siRNA transfected HBEC 5637 cells were collected from 6-well plates (containing 300,000 cells/well) using cell dissociation buffer (Life Technologies, Waltham, MA). The cells were lysed with RIPA lysis buffer supplemented with Halt™ Protease and Phosphatase Inhibitor Cocktail (Thermofisher ...
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bioRxiv - Immunology 2020Quote: ... Stimulated PBMCs and BAL cells were stained for dead cells using the LIVE/DEAD Fixable Near-IR Dead Cell Stain (Life Technologies, OR) and then surface-stained with the following antibodies ...
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bioRxiv - Immunology 2020Quote: Isolated splenic B cells (~10–20×106 cells) were stained first with 5 μL (5mM) Cell Trace Violet (C34557, Thermo Fisher Scientific) in 10 mL of PBS for 10 min at RT and let to recover in complete RPMI (+37°C ...
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bioRxiv - Immunology 2022Quote: ... cell supernatants were collected for cytokine/chemokine analyses and cells were stained with Fixable Aqua Dead Cell Stain Kit (Invitrogen/Thermo Scientific) for determination of cell surface expression of co-stimulatory molecules by FACS (FACSCanto II ...
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bioRxiv - Microbiology 2022Quote: ... RAW264.7 cells passaging was achieved by gentle cell scraping and seeding cells at a density of 3×106 cells/T75 flask (Nunc; Thermo Fisher Scientific).
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bioRxiv - Genomics 2020Quote: ... cells were dissociated using Accutase and cells were stained with LIVE/DEAD™ Fixable Far Red Dead Cell Stain Kit (Thermofisher, #L34973). The cells were washed with PBS ...
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bioRxiv - Immunology 2020Quote: ... BSC-1 cells were seeded at 1 x 105 cells per well in a sterile 12-well cell culture plate (Nunc, NY, USA) a day before the assay ...
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bioRxiv - Immunology 2021Quote: ... T2 target cells and Epstein-Barr Virus-transformed B lymphoblastoid cell lines (EBV-LCLs) cells were cultured in RPMI-1640 (Thermo Fisher Scientific) supplemented with 10% FCS and 1% penicillin/streptomycin (#P433 ...
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bioRxiv - Immunology 2022Quote: Wild-type C57BL/6 splenic-T cells are isolated using Stemcell EasySep™ Mouse T-cell Isolation kit (Stemcell #19851) and stained with Cell Trace Violet (ThermoFisher #C34571). These cells are then stimulated with anti-CD3/CD28 beads (Gibco #11456D ...
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bioRxiv - Cell Biology 2022Quote: ... cell counting was performed with trypan blue staining to assess cell viability (>90%) using an automated cell counter (CountessTM, Thermo Fisher Scientific). Conditioned medium was then collected and centrifuged for 10 min at 300 g and 4 °C ...
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bioRxiv - Immunology 2024Quote: Cell viability was measured by staining cells with LIVE/DEAD Fixable Aqua Dead Cell Stain or Blue Dead Cell Stain (Thermo Fisher Scientific) for 20 min at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Virions were transduced into BT549 breast carcinoma cells and stably expressing cell lines were produced by growing the cells in RPMI 1640 (Thermo Fisher #61870044) + 5 % FBS (Gibco #10500064 ...
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bioRxiv - Microbiology 2023Quote: ... RAW264.7 cells passaging was achieved by gentle cell scraping and seeding cells at a density of 3×106 cells/T75 flask (Nunc; Thermo Fisher Scientific).
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bioRxiv - Biophysics 2023Quote: In vitro cytotoxicity of the complexes was evaluated using U-2 OS cells and an LDH assay kit.42 Cells were counted with a Countess II Automated Cell Counter (ThermoFisher Scientific, USA) and seeded in 96-well plates at a density of 10,000 cells per well in 100 μL of full DMEM ...
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bioRxiv - Cancer Biology 2023Quote: ... The isolated cells were stained for cell viability using a Live/Dead Blue cell stain (L34962, Thermo Fisher Scientific, Waltham, MA, USA) and blocked with 4% bovine serum albumin (BSA) ...
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bioRxiv - Cancer Biology 2023Quote: LN-229 cells were seeded at 2×10^5 cells/well into in 6-well Nunc™ Cell-Culture Treated Multidishes (ThermoFisher, #140675) and incubated overnight in reduced serum media at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... HBVP cells were seeded at an initial concentration of 1.3X104 cells/cm2 in T-175 cell culture flasks (ThermoFisher Scientific, Cat.#: 178883) pre-coated with gelatin (4mg/mL in 1X PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... and respective control cells of CAL27 and FaDu head and neck squamous cell carcinoma cell lines were fluorescently dyed using CMFDA (Thermo Scientific, # C2925) and plated at a density of 10,000 cells per well in a 96-well plate ...
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bioRxiv - Immunology 2024Quote: ... Live cells were then stained for T cell lineage and activation markers: live/dead cell marker (1:200 dilution, Thermo Fisher, L23105), Alexa Fluor 647 anti-human CD3 (1:100 dilution ...
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bioRxiv - Molecular Biology 2024Quote: We expanded the cells to obtain 50 million cells and plated 12.5 million cells each onto four T-75 flasks (Thermo Fisher Scientific, #156499). We added 1μg/ml doxycycline to two of the flasks ...
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bioRxiv - Cancer Biology 2021Quote: ... and large cell clumps were removed by passing the cell solution through sterile 40 μm nylon mesh cell strainers (Fisher Scientific, Hampton, NH). Subsequently ...
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bioRxiv - Molecular Biology 2020Quote: ... The transfection complex and BxPC3 cells and PNAC-1 cells were then mixed together and seeded in 6-well (2 x 105 cells/well) cell culture plates (Nunc, Rochester, NY, USA) and cultured at 37°C for 24 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Bert Volgenstein at the Core Cell center Baltimore) cells and Hela cells were maintained in RPMI 1640 (Gibco; ref 61870-010; lot 1880320) supplemented with 10% FBS (Gibco ...
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bioRxiv - Microbiology 2021Quote: MT-2 and MT-4 cells (Japanese Collection of Research Bioresources Cell Bank; JCRB Cell Bank, Japan) were cultured in RPMI1640 medium (Gibco, Thermo Fisher Scientific, USA) with Fetal Bovine Serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... the TZM-bl cells (104 cells/well) in complete HiDMEM medium and PBMCs (105 cells/well) in complete RPMI (Thermo Scientific; OK, USA) supplemented with 10% FBS ...
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bioRxiv - Immunology 2021Quote: ... Live/dead cells were discriminated by staining cells with the LIVE/DEAD® Fixable Near-IR Dead Cell Stain kit (L10119, Thermo Fisher Scientific) (dilution 1:500) ...
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bioRxiv - Microbiology 2021Quote: ... the cells were transferred to a 24-well plate (5 × 105 cells/500 µL cell medium/well; Thermo Fisher Scientific, Waltham, MA, USA) overnight ...
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bioRxiv - Pathology 2023Quote: ... and the number of 4T1 cells was counted using an automated cell counter (Countess™ 3 FL Automated Cell Counter, Thermo Fisher Scientific). A cell resuspension with a final cell density of 1 × 106 cells/ml was prepared in PBS.
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bioRxiv - Microbiology 2023Quote: ... RAW264.7 cells passaging was achieved by gentle cell scraping and seeding cells at a density of 3×106 cells/T75 flask (Nunc; Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... processed to yield a single cell suspension and EpCAM (CD326) positive cells were isolated by magnetic cell sorting (Magnisort®, Thermo Fisher Scientific) using anti-CD326-biotin (13– 5791-82 ...
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bioRxiv - Microbiology 2022Quote: ... RAW264.7 cells passaging was achieved by gentle cell scraping and seeding cells at a density of 3×106 cells/T75 flask (Nunc; Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2023Quote: Cell dead and apoptosis were determined in PB3 and MDA-MB-231 cells using the Dead Cell Apoptosis Kit (Thermo Fisher Scientific #V35114) following different treatments according to the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2024Quote: ... any wells with cell death or rounded cells were noted then cells were lysed using the Cells-to-CT 1-step TaqMan Kit (Invitrogen cat no: A25602) according to the manufacturer protocol ...