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Citations for Thermo Fisher :
8451 - 8500 of 10000+ citations for Glutathione Fluorescent Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 1 μg of RNA was subjected to reverse transcription reaction with High-Capacity cDNA Reverse Transcription Kit (ThermoFisher). Quantitative RT-PCR reactions were measured on a Viia 7 Real-Time PCR system (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... DNA concentration was measured with 1×dsDNA HS Assay Kit on a Qubit 4 Fluorometer (Thermo Fisher Scientific). The four plate pools were pooled equimolarly to a final dilution of 2 nM and paired-end sequenced (2 × 250 ...
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bioRxiv - Molecular Biology 2023Quote: Up to 1 μg RNA was used for cDNA synthesis using the Superscript III Reverse Transcriptase kit (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... To enrich for MCL-1 binding protein immunoprecipitation was preformed using the Dynabeads Protein G IP Kit (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: The blaMUN-1 gene was cloned into pTOPO-kanR vector using the pCR-Blunt TOPO cloning kit (Invitrogen) using specific primers (MUN Fw ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were first incubated with LIVE/ DEAD Fixable Blue Dead Cell Stain kit (1:500, Thermofisher, cat#L23105) for 20 min ...
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bioRxiv - Plant Biology 2024Quote: Reverse transcription was carried out from 1 μg of purified RNA using the RevertAid RT kit (Thermo Scientific) with oligo(dT)18 primer ...
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bioRxiv - Plant Biology 2024Quote: ... For cDNA synthesis 1 µg RNA was used with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Relative expression of target genes was quantified by RT-qPCR (95 °C 3 min ...
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bioRxiv - Genetics 2024Quote: ... for 1-3 hours at 37C and subsequently cleaned up with GeneJet PCR clean up kit (Thermo Scientific) and resuspended in H2O ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μg of total RNA was reverse transcribed into cDNA using High Capacity cDNA transcription kit (ThermoFisher Scientific). Expression of marker genes was compared to expression of the two housekeeping genes GAPDH and ACTB ...
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bioRxiv - Biochemistry 2024Quote: ... We converted 1 µg of purified RNA to cDNA using a SuperScript IV VILO RT kit (Invitrogen, 11766050). Since apicoplast genes are extremely AT-rich76 and mRNA transcripts are not poly-adenylated or poly-uridylylated80 ...
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bioRxiv - Biochemistry 2024Quote: ... cDNA was synthetized using 1 µg RNA and the High Capacity cDNA reverse transcriptase kit (Applied Biosystems, 4368813). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Microbiology 2024Quote: ... and 1 μg RNA was used for cDNA synthesis with the High-Capacity cDNA Reverse Transcription Kit (ThermoFisher). Quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized from 1 μg total RNA using the SuperScript VILO cDNA Synthesis Kit (Thermo Fisher Scientific) per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: The labeled N-glycans released from RBD and the intestinal and lung tissue were analyzed by HILIC-UHPLC on a Thermo Scientific™ Vanquish™ UHPLC system coupled with fluorescent (FLD) (Thermo Scientific, Waltham, MA, USA) and ESI-MS detectors (Thermo Q Exactive Orbitrap) ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were visualised using secondary antibodies conjugated to the Alexa Fluor fluorescent dyes 594 and 488 (Invitrogen/Thermofisher catalogue numbers: A-11055 and A-21207).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The percentage of cells with oxidative stress and with damaged membranes were determined using the fluorescent probes CellROX®Green (Life Technologies Europe B.V., Zug, Switzerland) and propidium iodide (PI ...
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bioRxiv - Immunology 2022Quote: Cells collected from secondary lymph organs or cerebrospinal fluid of patients were stained with fluorescent-conjugated antibody: fixable viability dye (Thermo Fisher Scientific, Cat.No. 65-0866-14), CD45 (Biolegend ...
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bioRxiv - Cell Biology 2022Quote: ... respectively) and the fluorescent goat anti-rabbit Alexa-488 and anti-mouse Alexa-594 secondary antibodies were from Thermo Fisher (A-11008 and A-21203, respectively).
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bioRxiv - Pathology 2022Quote: Cells isolated as described in “Erythrocytic Compartment Preparation” and “Tissue Leukocyte Isolation” were incubated with Fc block (1:100) and LIVE/DEAD™ Fixable Yellow Dead Cell Stain Kit (ThermoFisher Scientific, #L34959; 1:5000 in PBS)(10min. ...
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bioRxiv - Immunology 2021Quote: ... This was followed by incubating the membrane with HRP-conjugated secondary antibodies for 2hrs before detection with the SuperSignal West Pico ECL substrate (Thermo Fisher Scientific #34577) and imaging on a Protein Simple FluorChem M.
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bioRxiv - Physiology 2019Quote: ... qPCR reactions were run on a fast-cycling real-time detection system (Applied Biosystems 7500 fast Real-Time PCR Systems, Life technologies AS), with a total volume of 10 µl ...
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bioRxiv - Cancer Biology 2019Quote: ... Quantitative real-time RT-PCR was carried out using Taqman Mix in an ABI Prism7900HT Sequence Detection System (Applied Biosystems, Foster City, CA). TaqMan Gene expression assays used were as follows ...
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bioRxiv - Cell Biology 2021Quote: ... The mRNA levels were determined by SYBR Green dye using an ABI 7500 sequence detection system with a reaction mixture that consisted of SYBR Green 2×PCR Master Mix (Applied Biosystems, CA, USA), cDNA template ...
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bioRxiv - Cancer Biology 2020Quote: ... Primers to ARF6 and 18s were designed using the Roche Universal Probe Library assay design center and qPCR was performed using Quanta PerfeCTa Supermix, Low Rox (Quanta BioScience, Gaithersburg, MD) on an ABI Prism 7500 Sequence Detection System (Life Technologies, Benicia, CA). Differences in expression between transfected and control non-transfected cells were determined using the comparative Ct method described in the ABI user manual relative to 18s for ARF6 ...
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bioRxiv - Cancer Biology 2020Quote: ... Primers to NFKB1 and 18s were designed using the Roche Universal Probe Library assay design center and qPCR was performed using Quanta PerfeCTa Supermix, Low Rox (Quanta BioScience, Gaithersburg, MD) on an ABI One Step Sequence Detection System (Life Technologies, Benicia, CA). miSCRIPT primer sets to hsa-pre-mir-218-1 (pre-mir-218-1) ...
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bioRxiv - Neuroscience 2022Quote: ... All dialysate samples were frozen at −80° before determination of dopamine concentrations by high-performance liquid chromatography (HPLC) with electrochemical detection (ESA/Thermo Scientific, Chelmsford, MA)
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bioRxiv - Cancer Biology 2022Quote: ... and target genes was analyzed by real-time RT-PCR after designing custom TaqMan® Small RNA Assays for isomiRs detection (Thermo Fisher Scientific). Total RNA was isolated from cells 48 hours post-transfection using TRIzol™ Reagent (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Immunoprecipitated protein was processed for western blotting as previously published (24) and developed with Clean-blot™ IP detection reagent (HRP) (Thermo Scientific, IL). Antibodies used for western blotting are listed in supplementary table S7.
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bioRxiv - Cell Biology 2022Quote: Click-iT™ Plus TUNEL assay for in situ apoptosis detection with Alexa Fuor™ 647 was performed according to the manufacturer protocol (ThermoFisher Scientific, C10619). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... and gene specific primers were used for PCR amplification and detection on a 7500 FAST Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific). Relative mRNA levels were determined by comparing the PCR cycle thresholds between cDNA of a specific gene and beta actin for mouse or histones for human (ΔCt).
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bioRxiv - Cell Biology 2019Quote: Real-Time PCR analysis was performed on an ABI Prism 7500 detection system (Applied Biosystem, CA, USA) using Power SYBR Green PCR Master Mix (Applied Biosystems, Thermo Fisher Scientific). Relative changes in gene expression were calculated using the 2-ΔΔCt method normalized against the housekeeping gene 18s ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Detection and quantitation of diclofenac was achieved in the positive ion mode with a TSQ Quantum Ultra Mass Spectrometer (ThermoFisher Scientific, Pittsburgh, PA), interfaced via an electrospray ionization (ESI ...
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bioRxiv - Cell Biology 2021Quote: ... SuperSignal West Pico PLUS Chemiluminescent Substrate was used for detection and blots were visualized with an iBright Western Blot (Thermo Fisher, Waltham, MA) imaging system.
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bioRxiv - Biochemistry 2021Quote: ... employing high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) utilising a Dionex DX600 system in combination with a CarboPac PA100® column (Dionex/ Thermo Scientific, Germany).
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bioRxiv - Immunology 2022Quote: ... Quantitative PCR was performed on CFX96 Touch Real-Time PCR Detection System (Bio Rad) using TaqMan™ Gene Expression Master Mix (Applied Biosystems™) and TaqMan probes (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... the membrane was exposed to an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) (PierceTM ECL Western; Thermo Scientific. Rockford, IL, USA). Bands were visualized and quantitated using the Chemi DocTM MP imaging system (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... Detection was achieved with a customized sector field ICP-MS (Element XR-2 with jet interface and desolvating injection, Thermo Scientific, Bremen Germany) and a diode array detector coupled to a metal-free HPLC system (Azury system ...
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bioRxiv - Microbiology 2024Quote: ... Detection of the murine-human chimera 72A1 detection was performed using goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody Alexa Fluor™ 488 tagged (Invitrogen #A-11001).
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bioRxiv - Cancer Biology 2024Quote: ... 48hrs after transfection the cells were fixed and stained for activated Caspase-3/7 using Apoptosis CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) according to manufacturers’ instructions ...
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bioRxiv - Cell Biology 2022Quote: ... a workspace with predefined gates and optimal PMT settings for the detection of 100 and 200 nm yellow-green (505/515) FluoSphere beads (Invitrogen, F8803 and F8848) was loaded ...
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bioRxiv - Microbiology 2024Quote: ... used for the duplex qPCR for canola blackleg detection (Fu et al. 2023) were analyzed for potential primer dimers using Multiple Primer Analyzer hosted by Thermo Fisher (https://www.thermofisher.com). One set without any predicted primer dimer was selected to be used in this study and named P-VL (Table 1) ...
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bioRxiv - Microbiology 2023Quote: ... Virus detection was performed by quantitative RT-qPCR on a QuantStudio® 5 Real-Time PCR system (Thermo Fisher Scientific, Waltham, MA, USA), according toconditions described in Guedes et al ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... and we next followed the procedures for RT-qPCR and CAG repeat analysis described in 27 with minor modifications: qPCR was performed on the Rotor-Gene 6000 Detection System (Corbett, Hilden, Germany) and QuantStudio 12K Flex (Thermo Fisher, Madrid, Spain), and each independent reaction was normalized to the level of Tbp ...
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bioRxiv - Microbiology 2023Quote: ... and (for detection of ISAV only) incubated with Alexa488-labelled goat anti-mouse IgG (A11001, Thermo Fisher Scientific, 5 μg/mL, 45 min). Signal was detected by a NovoCyte flow cytometer (Agilent).
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bioRxiv - Microbiology 2023Quote: ... The cells were fixed in 4% paraformaldehyde and washed with PBS followed by the terminal deoxynucleotidyl transferase-dUTP nick end labeling (Click-iT™ Plus TUNEL Assay for In Situ Apoptosis Detection, Thermo Fisher Scientific), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: The mRNA content of AMPKγ3 and TATA-box binding protein (TBP) was determined by real-time PCR using the QuantStudio 7 Flex detection system (Applied Biosystems, CA, USA) as previously described (33) ...
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bioRxiv - Plant Biology 2023Quote: ... Chemiluminescence was detected with ECL Prime Western Blotting Detection Reagent (Cytiva, GERPN2232) and SuperSignal™ West Femto Maximum Sensitivity Substrate (ThermoFisher Scientific, 34094).
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bioRxiv - Immunology 2023Quote: Metabolites were analyzed for relative abundance by high resolution accurate mass detection (HRAM) on two QExactive™ Orbitrap mass spectrometers (Thermo Fisher Scientific) coupled to Thermo Vanquish liquid chromatography systems as previously described (18) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The organic phase samples were analyzed by gas chromatography (GC) with flame-ionization detection (FID) in a TraceTM 1300 gas chromatograph (Thermo Fisher Scientific Co.) [87–89] ...