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Citations for Thermo Fisher :
8301 - 8350 of 10000+ citations for Glutathione Fluorescent Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... T-cells were co-cultured with the mCherry-Nucleus-7 MCF7 cells in the presence of CellEvent™ Caspase-3/7 Green Detection Reagent (ThermoFisher, C10423) in 96 well plates ...
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bioRxiv - Systems Biology 2019Quote: ... were detected using the appropriate secondary HRP-conjugated antibodies and visualized by enhanced chemiluminescence detection (Supersignal West Pico; Pierce - Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2020Quote: ... The concentration of nucleic acids was assessed by fluorimetric measurement through QuBit 3.0 and the amount of amplifiable DNA (sequencing-grade quality) was determined using a quantitative assay (TaqMan RNAseP detection assay) on a StepOnePlus instrument (both: Thermo Fisher Scientific). Samples were amplified using a custom-designed gene panel for breast cancer32 covering the most recurrent mutations33 ...
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bioRxiv - Neuroscience 2019Quote: ... Real-time PCR was run either on the ABI Prism 7000 Sequence Detection System using the Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) or on the LightCycler 480 System II using the LightCycler 480 SYBR Green I Master (Roche Diagnostics ...
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bioRxiv - Microbiology 2021Quote: ... samples were UV cross-linked and the biotin signal detected with HRP–conjugated streptavidin using the Chemiluminescent Nucleic Acid Detection Module (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... Real-time PCR was performed using Quant Studio Design and Analysis Real-Time PCR Detection System in conjunction with the Maxima SYBR Green qPCR Master Mix (Cat # K0221, Thermo Fisher Scientific) using specific primers for Stmn2 ...
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bioRxiv - Neuroscience 2020Quote: ... The qPCR was performed using the CFX96 Touch™ Real-Time PCR Detection System (Bio Rad) using Power SYBR Green PCR Master Mix (Applied Biosystems). The following primers were used for Cul3 - Cul3_Fwd ...
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bioRxiv - Bioengineering 2021Quote: ... The calibration beads allowed the detection of the MVs and EXOs using a 488/10 nm small particle side scatter filter (Invitrogen, Carlsbad, CA) on the BL1 channel and generate a size reference scale ...
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bioRxiv - Neuroscience 2021Quote: ... and heated for 10 mins at 70 °C (apart from samples meant for detection of amyloid) and run on 4–12% Bis-Tris precast gels (Nupage, Thermo Fisher Scientific) in MOPS buffer (Nupage ...
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bioRxiv - Physiology 2020Quote: ... as previously described (Danahay et al., 2015) with secondary antibodies to enable fluorescence detection of both proteins (Alexa Fluor; Thermo Fisher, UK). The MUC5AC+ stained area was visualised using a Zeiss Axiovert epifluorescence microscope with a motorised stage that was used to image the same 9 regions of interest on each insert ...
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bioRxiv - Molecular Biology 2022Quote: The slides were stained for DNA detection with Hoechst 33342 (Life Technology) and mounted in ProLong Gold antifade reagent (Life Technologies, P36934).
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bioRxiv - Molecular Biology 2022Quote: ... The slides were stained for DNA detection with Hoechst 33342 (Life Technology) and mounted in ProLong Gold antifade reagent (Life Technologies, P36934).
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bioRxiv - Molecular Biology 2020Quote: ... Commercially available ELISAs for quantitative detection of human total IgG were used according to manufacturer’s protocols (Thermo Fisher Scientific, Waltham, MA, U.S.A.). All plates were read in a microtiter plate reader at 450 nm and optical density values were used in statistical analyses.
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bioRxiv - Cell Biology 2021Quote: ... then incubated with 25 µL of the Click-iT EdU detection mix (prepared as per manufacturer’s instructions using Alex Fluor 455®; Life Technologies, ThermoFisher) for 1 hr in the dark ...
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bioRxiv - Systems Biology 2022Quote: ... Diluted cDNA was used for qPCR that was done by using the StepOnePlus sequence detection systems (Applied Biosystems, Foster City, CA, USA) with SYBR Green Mastermix (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2022Quote: ... The membrane stained with Clean-Blot™ IP detection reagent was treated with SuperSignal™ West Pico PLUS Chemiluminescent Substrate (ThermoFisher 34577) for at least 5 min at room temperature before imaging ...
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bioRxiv - Biochemistry 2019Quote: Samples were analyzed using liquid chromatography on a Dionex Ultimate 3000 HPLC system with on-line mass spectrometry detection (Thermo Scientific, USA). The separation was achieved using a HILIC column iHILIC-Fusion (150 × 2.1 mm ...
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bioRxiv - Cancer Biology 2019Quote: ... Rox (Quanta Biosciences) according to the manufacturer’s protocol using the ABI PRISM 7900HT Sequence Detection System (Applied Biosystems, Foster City, California, USA). The target CT values were normalized to b-actin ...
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bioRxiv - Pathology 2020Quote: ... and mRNA expression was analyzed using total RNA from tissues and cells and the Applied Biosystems 7000 sequence detection system (Applied Biosystems, UK), SYBR Green ...
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bioRxiv - Cancer Biology 2020Quote: ... qPCR reactions were performed on a CFX384 Touch Real-Time PCR Detection System using 2X Power SYBR Green PCR Master Mix (Thermo Fisher Scientific). For each condition ...
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bioRxiv - Cell Biology 2019Quote: ... Real-time PCR was performed using Quant Studio Design and Analysis Real-Time PCR Detection System in conjunction with the Maxima SYBR Green qPCR Master Mix (Cat # K0221, Thermo Fisher Scientific) using specific primers for Stmn2 ...
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bioRxiv - Molecular Biology 2021Quote: ... After incubation for 1 h at 37 °C the DNA products were analyzed by electrophoresis on a denaturing polyacrylamide gel (10%) and detected by chemiluminescent detection (Thermo Fisher Scientific). To semi-quantify the level of dCTP incorporation ...
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bioRxiv - Cancer Biology 2022Quote: ... At time of treatment the cells were also treated with 2μM CellEvent caspase-3/7 green detection reagent (C10423; Invitrogen, ThermoFisher Scientific, Inc), which was used to measure apoptosis ...
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bioRxiv - Plant Biology 2022Quote: ... MitoTracker dye was excited at 644 nm with a detection range of 578-696 nm for MitoTracker™ Deep Red FM (Invitrogen M22426). For GUS staining ...
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bioRxiv - Neuroscience 2022Quote: ... One-step real-time RT-PCR assays targeting SARS-CoV-2 nucleocapsid (N) coding region and subgenomic RNA were run on ABI 7900HT Sequence Detection Systems (Thermo Fisher Scientific), as previously reported25.
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bioRxiv - Plant Biology 2022Quote: ... The DNA-protein complex was blotted to a positively charged nylon membrane and imaged using Chemiluminescent Nucleic Acid Detection Module (Thermo Fisher Scientific). The primers used are listed in Supplemental Table S1.
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bioRxiv - Cancer Biology 2022Quote: ... qRT-PCR reactions were performed on a CFX384 Touch Real-Time PCR Detection System using 2× Power SYBR Green PCR Master Mix (Thermo Fisher Scientific). For each condition ...
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bioRxiv - Cancer Biology 2022Quote: ... Secondary ab with the correspondent species specificity were used for the signal detection: donkey anti-rabbit Alexa Fluor Cy3 and 488 conjugated secondary ab (Invitrogen, A32790, A21207) for IF ...
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bioRxiv - Pathology 2024Quote: ... Primers for Sh3bgr cryptic exon detection (Table 4) were used to amplify the targets using the DreamTaq 2x master mix (Thermo Fisher, K1081). The PCR reaction settings using a Bio-Rad C1000 Touch Thermal Cycler were 95 °C for 60s ...
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bioRxiv - Cancer Biology 2024Quote: ... and Zeb1 was evaluated by qRT-PCR assay which is based on SYBR® Green gene expression analysis detection system (Thermo Scientific). For loading control ...
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bioRxiv - Plant Biology 2023Quote: ... The qRT-PCR amplification was carried out using the ABI Prism 7500 Fast sequence detection system (Applied Biosystems, Foster City, CA, USA) with Fast SYBR™ Green PCR Master Mix (Applied Biosystems™ ...
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bioRxiv - Biochemistry 2023Quote: ... Detection of bound Af2-IL was performed using a rat anti-human IL-2 mAb Alexa Fluor488 conjugate (Invitrogen, Carlsbad CA, USA). Cell nuclei were counterstained with DAPI (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... Our limit of detection (LoD) was calculated using an average of each species probing for Pan Eukaryotic 18S (ThermoFisher Scientific, MA USA) with a cycle threshold of 35 ...
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bioRxiv - Bioengineering 2023Quote: ... A small amount of PCR product was taken for concentration detection using a Qubit 4.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA). The PCR product quality was also assayed by electrophoresis on a 1% agarose gel at 120 V for 30 minutes followed by visualization with a gel imaging system.
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bioRxiv - Cell Biology 2022Quote: ... The following horseradish peroxidase (HRP)-coupled secondary antibodies were used for detection using Pierce™ ECL Western Blotting-Substrate (Thermo Fisher Scientific): HRP-conjugated goat anti-mouse IgG (ab6789 ...
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bioRxiv - Biochemistry 2022Quote: ... The MS analysis for quick detection of enzyme assay products was conducted on the TSQ Quantum Access Max mass spectrometer (Thermo Fisher Scientific) operated in negative ionization mode with a full scan range of 100-300 m/z ...
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bioRxiv - Genetics 2023Quote: ... of the complementary T7/M13 primer during PCR and hence detection during capillary electrophoresis on ABI 3730 Genetic Analyzer (Applied Biosystems Inc.).
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bioRxiv - Neuroscience 2023Quote: hpRP chromatography was performed as previously reported using a Dionex UltiMate 3000 HPLC system with a built-in micro-fraction collector and UV detection (Thermo Fisher Scientific)32 ...
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bioRxiv - Molecular Biology 2023Quote: ... quantitative real-time PCR was performed with an Applied Biosystems Prism 7500 Fast Sequence Detection System using the TaqMan fast universal PCR master mix reagents (Applied Biosystems, 4352042). The mRNA expression levels were analysed using the 2–ΔΔCt method.
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bioRxiv - Molecular Biology 2023Quote: ... 800 μL of the supernatant was transferred to a 2 mL vial for detection by GCMS-QP2010 Ultra (Thermo Scientific, Massachusetts, USA). The specific parameters were as follows ...
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bioRxiv - Evolutionary Biology 2024Quote: The detection method is performed using a high-performance anion-exchange chromatography (HPAEC) coupled with pulsed amperometric detection (PAD) (DIONEX ICS6000 system, Thermo Fisher Scientific). The system is equipped with a CarboPac-PA1 guard column (2 x 50 mm ...
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bioRxiv - Cell Biology 2024Quote: The amount of reactive oxygen species (ROS) produced within the cell was measured using CellROX™ Deep Red Reagent for oxidative stress detection (Invitrogen, USA). The cells were cultured in glass slides (ibidi ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were washed in T-TBS and incubated with the corresponding HRP-conjugated secondary antibody for detection by the enhanced chemiluminescence SuperSignal® West Femto Maximum Sensitivity Substrate (#34095, Thermo Scientific). GAPDH was used as a cellular loading control ...
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bioRxiv - Neuroscience 2024Quote: ... Cycling and detection were carried out using an Applied Biosystems 7500 Real Time PCR System and data quantified using Sequence Detection Software Version 1.4 (Applied Biosystems, Carlsbad, CA). PCR was performed for a total of 40 cycles (95° for 15 sec ...
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bioRxiv - Cancer Biology 2024Quote: ... After 3× washing of 5 min in TBS-T antibody detection was performed by HRP-ECL chemiluminescence reaction (#32109, Thermo Fisher Scientific) using the Fusion FX 7 (Vilber Lourmat GmbH ...
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bioRxiv - Bioengineering 2024Quote: ... flow throughs and washes from StEVAC method were suspended in 20 µl of Exosome-human CD81 flow detection beads (Thermo Scientific, 10622D) and incubated at 4°C overnight while rotating ...
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bioRxiv - Cell Biology 2022Quote: ... in a 1:1 ratio and were transfected into 100,000 3T3L1 cells by electroporation (#MPK1025 Neon transfection kit, Invitrogen, three pulses at 1650 V, 10 ms). Editing efficiency of bulk cell pools was initially confirmed by T7E1 (T7 Endonuclease 1 ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 µg of total RNA was reverse-transcribed using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, USA). qRT-PCR reactions were performed using SYBR green Mastermix (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... quantified using Nanodrop and 1 µg was converted to cDNA (High Capacity cDNA Reverse Transcription Kit - Applied Biosystems). The quantitative real-time PCR amplification -95°C (10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 µl of each sample was quantified using the Quant-iT High-Sensitivity dsDNA Assay Kit (Thermo Fisher). The quantity of cDNA for one replicate sample of WT 28°C was too low and was excluded from further experiments ...