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Citations for Thermo Fisher :
801 - 850 of 8899 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... Elution fractions were dialyzed overnight in 1 x PBS and 2 mM EDTA using 10 kDa MWCO dialysis cassettes (Cat # 66380, ThermoFisher Scientific, Waltham, MA).
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bioRxiv - Biochemistry 2024Quote: ... dextrans were purified by extensive dialysis in 3.5 KDa or 20 KDa cutoff Side-A-Lyzer dialysis cassettes (Thermo Fisher 66330 and 66003) against PBS.
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM DTT) for 24 to 36 hours with gentle stirring at 4°C using a Side-A-Lyzer dialysis cassette (Thermo Scientific, Prod# 66380). The dialyzed protein was concentrated using Amicon 10K and 30K columns (Millipore ...
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bioRxiv - Molecular Biology 2020Quote: ... Gels were stained with 1 mL 1/1,000 SYBR Gold Nucleic Acid Gel Stain (ThermoFisher Scientific) and imaged with the GelDoc™ XR+ system (BioRad).
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bioRxiv - Cell Biology 2019Quote: Proteins were separated by polyacrylamide gel electrophoresis using 4–12% Bis-Tris Bolt gels (Thermo Fisher) and transferred onto nitrocellulose membranes (0.45 µm ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were visualized on a 1.5% agarose gel stained with SYBRsafe DNA gel stain (Invitrogen) and visualized under UV transillumination.
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bioRxiv - Neuroscience 2020Quote: ... run on Novex pre-cast mini gels (NuPAGE 4-12% Bis-Tris Gels, NP0322BOX, Thermo Scientific) in 1 x MOPS buffer and transferred onto PVDF membranes using a Hoefer wet transfer tank (TE22 ...
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bioRxiv - Genomics 2021Quote: ... The samples were purified using 2% E-Gel EX precast agarose gels (Thermo Fisher Scientific, G401002). Each sample was visualized on a Bioanalyzer (Agilent Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... as previously reported13 and resolved by gel electrophoresis using Novex 4-12% Bis-Tris Gels (Invitrogen), transferred to a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Cancer Biology 2019Quote: ... Reaction products were run on a 2% agarose gel containing Syber Safe DNA gel stain (Invitrogen) to ascertain correct primer amplification (Supplementary Fig ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were resolved by SDS-polyacrylamide gel electrophoresis (NuPAGE 4-12 % Bis-Tris protein gels, Invitrogen) and detected by immunoblotting.
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bioRxiv - Plant Biology 2021Quote: ... PCR products were excised and purified from agarose gel using GeneJET Gel Extraction Kit (Thermo Fisher) and cloned into Gateway™ pENTR™ 4 by mixing the linearized vector backbone and PCR product in a 1:1 ratio using Gibson assembly (NEB) ...
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bioRxiv - Neuroscience 2020Quote: ... prior to separation by SDS-PAGE gel electrophoresis using precast 4-12 % Bis-Tris gels (Invitrogen). Proteins were transferred to nitrocellulose or PVDF membranes prior to blocking in 5 % milk/PBST (0.05 % Tween-20 ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were run on 1% agarose gel and purified using gel extraction Kit (Thermo Scientific). To generate donor plasmids ...
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bioRxiv - Microbiology 2021Quote: ... The PCR product was purified using E-Gel™ SizeSelect™ II 2% agarose gel (Invitrogen). RNA-seq library for 300 bp insert size was constructed following the manufacturer’s instructions (NEBNext Ultra™ II DNA Library Prep Kit) ...
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bioRxiv - Immunology 2022Quote: ... Denatured samples were analysed by SDS gel electrophoresis using 4-12% Bis-Tris gels (NuPAGE, Invitrogen), transferred to a nitrocellulose membrane (NuPAGE ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were resolved on 15% Tris-Glycine gels or NuPAGE Bis-Tris gels (NP0343, Invitrogen), and transferred to PVDF membrane (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... The fragments were run on 2% E-Gel™ EX agarose gels (Thermo Fisher scientific, G521802) and 150-600 bp fragments were excised ...
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bioRxiv - Plant Biology 2021Quote: SDS-PAGE gel electrophoresis and protein gel blotting with PVDF membranes (Thermo Scientific catalogue no 88520) were performed as described (Lauber et al. ...
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bioRxiv - Synthetic Biology 2021Quote: ... Amplification products were run on 0.7% agarose gels stained with SYBR Safe DNA Gel Stain (Invitrogen). The bands of interest were then excided ...
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bioRxiv - Genetics 2019Quote: ... The gel was stained using SYBR Safe DNA gel stain (Invitrogen Life Technologies, Carlsbad, CA, U.S.A.) and specific bands were cleaned using NucleoSpin gel clean-up kit (Takara Bio ...
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bioRxiv - Microbiology 2019Quote: ... RFPs and fragmented RNA were collected by gel extraction from a 15% denaturing NuPAGE gel (Invitrogen).
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was gel-size selected by running samples on a precast 6% TBE-urea gel (Invitrogen) at 180V for 40 min and isolating the region between 75 and 200 nt ...
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bioRxiv - Genomics 2021Quote: ... Denatured samples were loaded onto a 1% MOPS gel with 1x Denaturing Gel Buffer (ThermoFisher Scientific) and ran at 102V for 60 min ...
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bioRxiv - Biochemistry 2021Quote: ... A gel chamber with a 26 well 4−12% NuPAGE Bis-Tris Gels (Thermo Fisher Scientific) was assembled and filled with 1X NuPAGE MES-SDS Running Buffer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... the samples were loaded on a 4-20% gradient gel (Novex Tris-Glycine Mini Gels, ThermoFisher). Proteins were then electrotransferred to an Immobilon®-P polyvinylidene fluoride (PVDF ...
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bioRxiv - Neuroscience 2021Quote: Gel electrophoresis was performed using 4-12% or 12% Bolt Bis-Tris Plus gels (Thermo Scientific) for 35 min at 165 V ...
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bioRxiv - Immunology 2022Quote: ... Protein gel electrophoresis was performed on ice using NuPAGE 4-12% Bis-Tris Protein Gels (Invitrogen) at 135V ...
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bioRxiv - Genetics 2022Quote: ... The PCR amplicons were visualized on 1% agarose gel with SYBR Safe DNA gel stain (Invitrogen) and purified with Gel/PCR DNA Fragment Extraction Kit (Geneaid Biotech ...
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bioRxiv - Microbiology 2023Quote: ... Gel was stained using Pro-Q™ Emerald 300 Lipopolysaccharide Gel Stain Kit (ThermoFisher cat. P20495) and visualized using a BioRad imager.
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were size selected in a 2% E-Gel Agarose EX Gels (Life Technologies, Darmstadt, Germany), cut out in the range of 300–800LJbp ...
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bioRxiv - Neuroscience 2022Quote: ... prior to separation by SDS-PAGE gel electrophoresis using precast 4-12% Bis-Tris gels (Invitrogen). Proteins were transferred to nitrocellulose or PVDF membranes prior to blocking in 5% milk/PBST (0.05% Tween 20 ...
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Dual targeting factors are required for LXG toxin export by the bacterial type VIIb secretion systembioRxiv - Microbiology 2022Quote: ... Protein visualization on SDS-PAGE gels was done with the SYPRO Red protein gel stain (Invitrogen). The gel was rinsed briefly in DI water before being stained for one hour with 1:5000 SYPRO Red (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... Libraries were then size-selected using the E-Gel EX 2% agarose gel (Cat. # G402022, Invitrogen) and the E-Gel Power Snap Electrophoresis System (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... and integrity was investigated by visual inspection using agarose gel electrophoresis (E-gel EX 1%, Invitrogen). All RNA samples passed the quality control and was stored at −80°C.
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bioRxiv - Cancer Biology 2023Quote: ... 3% agarose gels were prepared with a 1:20000 dilution of SYBRSafe DNA gel stain (Invitrogen). Once complete the PCR products were loaded onto the gel and the electrophoresis was run on a Powerpac Electrophoresis system (BioRad ...
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bioRxiv - Bioengineering 2023Quote: ... Amplified libraries between 250-400 bp were recovered using 2% E-Gel pre-cast gels (ThermoFisher), purified with Zymoclean Gel DNA Recovery Kit (Zymo Research) ...
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CXCL17 binds efficaciously to glycosaminoglycans with the potential to modulate chemokine signallingbioRxiv - Immunology 2023Quote: ... using 12% NuPAGE Bis-Tris Mini Gels and MES buffer on a Mini Gel Tank (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... and loaded in a 2% Agarose gel containing SYBR Safe DNA gel stain (Thermofisher Scientific, #S33112) and ran for 45 min electrophoresis at 120V ...
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bioRxiv - Cancer Biology 2023Quote: ... and gel purified it using the Gene JET Gel extraction kit (Thermo Fisher Scientific; Cat #K0692). Oligos encoding sgRNA sequences were phosphorylated ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were counterstained with Fluoro-gel II containing DAPI (Fluoro-Gel, Fisher Scientific Intl INC, PA). Immunostaining images were taken using a Leica inverted microscope with a TCS SPEII confocal module and processed using LAS X software (Leica Microsystems Inc ...
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bioRxiv - Cell Biology 2023Quote: ... the gels were stained with Pro-Q Diamond phosphoprotein stain gel solution (Thermo Fisher Scientific, P33300) according to the manufacturer’s instruction with the exception that 1,2-propanediol was used instead of acetonitrile in the destaining step ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Amplified DNA fragments were purified from the agarose gel using GeneJET Gel Extraction Kit (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR product was then isolated by running on E-Gel EX 1% Agarose Gels (ThermoFisher G401001), excising the band at the expected length ...
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bioRxiv - Biochemistry 2024Quote: ... PCR products were gel purified using the E-Gel Power Snap Plus Electrophoresis System (ThermoFisher #G9301). 5 μL of PCR products was run on a 2% E-gel EX Agarose Gel (ThermoFisher #G401002 ...
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bioRxiv - Developmental Biology 2023Quote: ... The size of the amplicons was verified by agarose gel electrophoresis (E-gel 4%, Life Technologies). The relative expression level of each gene was normalized to two housekeeping genes as recommended by the MIQE guidelines (47) ...
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bioRxiv - Biophysics 2024Quote: Gel-bands were digested with the In-Gel Tryptic Digestion Kit (Thermo Fisher Scientific, Dreieich, Germany) with minor adaptations ...
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bioRxiv - Genetics 2024Quote: ... The obtained products were gel purified using the GeneJET gel extraction kit (Thermo Fisher Scientific™) and sent for sequencing using Illumina MiniSeq™ at the Genome Center of Eastern Finland.
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bioRxiv - Microbiology 2024Quote: ... Protein samples were separated using polyacrylamide gel electrophoresis (PAGE) in NuPAGE (4–12%) gels (Life Technologies), then transferred to nitrocellulose membranes and blocked in 4% w/v skim milk powder in Phosphate Buffered Saline (PBS) ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were subjected to SDS gel electrophoresis using 8% or 4-12% Bis-Tris gels (Invitrogen) and transferred nitrocellulose membranes for immunoblotting ...