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Citations for Thermo Fisher :
951 - 1000 of 9427 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 4-20% Tris-Glycine gels (Invitrogen) were used and electroblotting carried out at 25V and 1.3A for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Bis-Tris NativePAGE gels (Invitrogen BN1001BOX) with NativePAGE anode buffer (Invitrogen 2001 ...
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bioRxiv - Molecular Biology 2020Quote: The eluted protein was mixed with TEV protease in a 100:1 (w/w) and loaded into a 3 kDa MWCO dialysis cassette (Thermo Fisher Scientific, United Kingdom). Cleavage of the His6-thrombin-TEV tag was carried out overnight at 4 °C in lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... and subsequently into tissue cassettes and embedded in paraffin using an automatic tissue processor (Thermo Scientific Excelsior AS, Thermo Fisher Scientific Ltd., Waltham, USA). The sequence of fixation and embedding steps applied are summarised in Supplementary Table S4 ...
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bioRxiv - Genetics 2022Quote: ... Then the pBS-eggpl-arm was linearized by PCR and linked to the GFP-6HA cassette with Gibson Assembly Kit (Thermo Fisher, cat. no. A46624), and that produced the final donor construct ‘pBS-CG32814-GFP-6HA’ (supplementary Fig.3 A).
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bioRxiv - Microbiology 2022Quote: ... the 10910 base pair region encompassing vlsE and silent cassettes vls2-vls16 was amplified using Platinum™ SuperFi™ DNA Polymerase (Thermo Fisher Scientific) and primers YN-LI_266 and YN-LI_267 (Table 6 ...
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bioRxiv - Cell Biology 2024Quote: ... p24 sequences were transferred into modified Gateway destination vector pTSGW (UASt-Signal peptide of Tango1-GFP-Gateway cassette) (Yang et al., 2021) through Gateway LR recombination using LR Clonase II Plus enzyme (Thermo Fisher Scientific, cat # 12538120) to obtain the desired plasmids.
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bioRxiv - Cell Biology 2023Quote: ... Cells transduced with plasmids containing puromycin and gentamicin resistance cassettes were selected in media containing 2 µg/ml puromycin (Invitrogen, Waltham, MA, United States) or 4 mg/ml of geneticin ...
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bioRxiv - Microbiology 2023Quote: ... or 100 mM Tris-Cl buffer containing 10% sucrose at pH 7.4 (LNP2) using the Slide-A-LyzerTM G2 dialysis cassettes with the molecular weight cut-off of 10 kDa (Thermo Fisher Scientific, Carlsbad, CA). After dialysis ...
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bioRxiv - Bioengineering 2023Quote: ... for 2 hours (for in vitro experiments) or 4 hours (for in vivo experiments) in 10K MWCO Slide-A-Lyzer dialysis cassettes (Thermo Fisher Scientific, catalog #87730).
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bioRxiv - Plant Biology 2024Quote: ... The indicated total protein amount was loaded on SDS-PAGE gels (4-20% Mini-protean® TGX™ precast gels, [Bio-Rad], NuPAGE 4-12% Bis-Tris gels [Thermofisher], or self-cast minigels) and blotted on PVDF Immobilon-P membrane (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... and the non-labeled DNAs in the gel were stained with SYBR Gold Nucleic Gel Stain (Invitrogen) and photographed under a 470-nm blue LED.
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bioRxiv - Cancer Biology 2021Quote: ... in RIPA buffer and separated on SDS-PAGE gels (NuPAGE 4– 12% Bis-Tris Protein Gels, Invitrogen). Membranes were probed using the following primary antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... SDS-PAGE electrophoresis was performed using 4-12% gradient bis-tris gels or 16% tricine gels (Invitrogen) and proteins were transferred to nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein samples were separated by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) (3-8% gradient gels, Invitrogen) and subsequently transferred onto nitrocellulose membranes ...
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bioRxiv - Genetics 2019Quote: ... 4-12% gradient SDS-polyacrylamide gel electrophoresis (SDS-PAGE) was performed using NuPage gels from Thermo Fisher. Met/Cys-deficient Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cell Biology 2019Quote: ... The gels were run in an Owl™ EasyCast™ B1 Mini Gel Electrophoresis System (Thermo Scientific). The RNA purity and quantity were assessed by loading 1.5 µl of each sample onto a NanoDrop™ 2000c Spectrophotometer and reading the A260 nm emission ...
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bioRxiv - Cancer Biology 2020Quote: ... Annealed oligos were cloned into a gel purified (GeneJet Gel Extration Kit; Thermo Fisher Scientific, Waltham, MA), dephosphorylated (Fast AP ...
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bioRxiv - Biochemistry 2020Quote: ... the vector and insert amplicons were gel-purified (GeneJet Gel Extraction Kit, Thermo Scientific Cat No. K0691) according to the manufacturer’s instructions and inserted using circular polymerase extension cloning [24] ...
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bioRxiv - Cell Biology 2021Quote: ... Gel electrophoresis was performed and the gels were stained with GelCode Blue Stain reagent (Thermo-Fisher Scientific).
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bioRxiv - Synthetic Biology 2021Quote: ... and all E-gels were imaged using the E-gel power snap camera (ThermoFisher Scientific, Cat #: G8200). Unless otherwise stated ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were confirmed by 1.5% agarose gel electrophoresis stained with SYBR Safe DNA gel stain (Invitrogen) and visualized under blue light ...
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bioRxiv - Microbiology 2022Quote: ... were analyzed by SDS gel electrophoresis on NuPAGE™ 4-12% Bis-Tris gels (Thermo Fisher Scientific) and blotted.
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bioRxiv - Microbiology 2020Quote: ... RNA products were separated by gel electrophoresis in 10% TBE-Urea gels (Invitrogen, Carlsbad, CA, United States) at 180 V for 45 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Integrity of gDNA was analyzed with a 1 % E-Gel™ EX Agarose Gel (Thermo Fisher Scientific). To obtain DNA from formalin-fixed ...
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bioRxiv - Neuroscience 2020Quote: ... the PCR products were run on a 3% agarose gel with SYBR Safe DNA gel stain (Invitrogen). The genotyping primers are ...
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bioRxiv - Bioengineering 2021Quote: ... SDS-PAGE gel electrophoresis of cell free supernatant was carried out using Novex mini gel system (Invitrogen) with Nu-PAGE 10 % Bis-Tris gels and MES-SDS running buffer ...
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bioRxiv - Microbiology 2021Quote: ... gels were stained in the dark using 4μl of SYBR gold nucleic acid gel stain (Invitrogen™) in 400ml 1x TAE buffer ...
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bioRxiv - Microbiology 2022Quote: PCR products were separated by gel electrophoresis with fluorophore (SYBR Safe DNA Gel Stain, Invitrogen, ThermoFisher Scientific) in a 1% agarose gel (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: PCR products were separated by gel electrophoresis with fluorophore (SYBR Safe DNA Gel Stain, Invitrogen, ThermoFisher Scientific) in a 1% agarose gel (Invitrogen ...
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bioRxiv - Physiology 2022Quote: ... 15µl samples were run on 2% agarose gels with 10µl SYBR®Safe DNA Gel Stain (Invitrogen) in TBE buffer against a 100 bp DNA ladder (GeneRulerTM ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were gel purified using GeneJET Gel Extraction and DNA Cleanup Micro kits (Thermo Fisher Scientific), cloned into the pGEM T-Easy vector (Promega ...
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bioRxiv - Cell Biology 2019Quote: ... The samples were loaded on a 1% agarose gel containing SYBRTM Safe DNA Gel stain (#S33102, Invitrogen) with 1kb ladder (#N3232S ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were analyzed by electrophoresis in pre-cast gels 4-12% polyacrylamide-SDS gels (Life Technologies NP0322) using the corresponding migration buffer (MOPS NP0001) ...
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bioRxiv - Physiology 2020Quote: ... and the nucleotide chains were extracted from the gel using the GeneJET Gel Extraction Kit (Thermo Scientific). Overhang adenines were added to the 3′-ends of PCR products using Dynazyme II DNA polymerase (Thermo Scientific ...
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bioRxiv - Immunology 2020Quote: ... Ghost protein samples were fractionated by gel electrophoresis using NuPage 4-12% Bis-Tris gel (Invitrogen, NP0312BOX) and transferred by western blotting (30V ...
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bioRxiv - Microbiology 2020Quote: ... separated by polyacrylamide gel electrophoresis (PAGE) using 8–16% Tris-Glycine polyacrylamide gradient gels (Thermo Fisher Scientific) with Tris-Glycine SDS running buffer (25mM Tris ...
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bioRxiv - Genomics 2020Quote: ... PCR products were visualised by 1.5% agarose gel electrophoresis stained with SYBR Safe DNA gel stain (ThermoFisher) and imaged with a BioDoc-It imaging system (UVP) ...
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bioRxiv - Immunology 2020Quote: ... excised and extracted from agarose gel using GeneJET Gel Extraction and DNA Cleanup Micro Kit (ThermoFisher, USA), and assembled together using NEBuilder® HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Bioengineering 2022Quote: ... pBAE:miR-29b complexes were freshly prepared and loaded in 2% E-Gel Precast Agarose Gels (Thermo Fisher), run following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Amplicons were visualized on 1.5% agarose gels stained with SYBR Safe DNA Gel Stain (Invitrogen, Waltham, MA), and imaged under UV light.
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bioRxiv - Microbiology 2022Quote: Protein extracts were resolved by SDS-polyacrylamide gel electrophoresis on 4-12 % gradient NUPAGE-PAGE gel (Invitrogen) with MOPS running buffer and transferred to cellulose membranes (GE Healthcare ...
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bioRxiv - Molecular Biology 2022Quote: ... thus gel extraction was performed on each pool using the GeneJET Gel Extraction Kit (Thermo Scientific, UK) with elution in 20 μl ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were run on 1% agarose gels and extracted using Fermentas Gel Extraction Kit (Invitrogen, K0692). Sanger sequencing was performed by Eurofins Genomics.
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bioRxiv - Neuroscience 2022Quote: ... with the gels subsequently stained using Pro-Q ® Diamond Phosphoprotein Gel Stain following manufacturer’s protocol (Invitrogen) and scanned again ...
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bioRxiv - Molecular Biology 2022Quote: Proteins were resolved by SDS-PAGE on precast gradient gels (4-12% Bis-Tris Bolt gels, ThermoFisher) and transferred to Hybond-C membrane (Amersham Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... Insert sample was run on a 20% native PAGE gel (Novex TBE Gels, Thermo Fisher Scientific, EC63155BOX), and the band (33 bp ...
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bioRxiv - Molecular Biology 2024Quote: ... gels were then incubated with 10,000-fold diluted SYBR Gold nucleic acid gel (Life Technologies, Cat# S11494) in 1X TBE buffer for 30 minutes at room temperature (RT) ...
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bioRxiv - Molecular Biology 2024Quote: ... the mono-nucleosomal DNA was enriched by gel extraction by the PureLink Quick Gel Extraction kit (Invitrogen). Sequencing libraries were prepared as in [34] with the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB).
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bioRxiv - Biophysics 2024Quote: ... Excised gel bands were digested using the In-Gel Tryptic Digestion Kit (Thermo Fisher Scientific, Dreieich, Germany). The gel pieces were destained ...