Labshake search
Citations for Thermo Fisher :
651 - 700 of 8899 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... The gels were stained with the Invitrogen SYPRO Ruby protein gel stain (ThermoFisher Scientific).
-
bioRxiv - Neuroscience 2023Quote: ... The gel tank was assembled and a 4-12% Bis-Tris Gel (NP0335BOX, Invitrogen) was loaded with ladder 9 and 25μL of sample per lane ...
-
bioRxiv - Developmental Biology 2024Quote: ... PCR products were gel-extracted using the PureLink Quick Gel Extraction kit (Invitrogen, K210012). Amplicon concentrations were quantified with the QubitTM dsDNA HS Assay kit (Invitrogen ...
-
bioRxiv - Microbiology 2023Quote: ... DNA bands were visualized using E-Gel™ EX Agarose Gels (2%, Invitrogen #G401002) under UV light.
-
bioRxiv - Developmental Biology 2024Quote: ... The gels were transferred onto PVDF membranes using iBlot PVDF gel transfer (ThermoFisher Scientific). The membranes were then immersed in a blocking solution (5% nonfat milk in TBST ...
-
bioRxiv - Molecular Biology 2023Quote: Proteins were separated on gel (NuPAGE Novex 4-12% Bis-Tris gel, Life Technologies), in NuPAGE buffer (MES ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... Gel electrophoresis was performed using Bolt 4–12% Bis-Tris Plus gels (Life Technologies) with 25μg of protein loaded per well ...
-
bioRxiv - Cell Biology 2024Quote: ... Cell protein extracts were resolved by Bis-Tris polyacrylamide gel electrophoresis gels (Life Technologies) and run in 1 X MOPS buffer (Life Technologies) ...
-
bioRxiv - Cell Biology 2024Quote: ... all reagents underwent filtration through Detoxi-Gel Endotoxin Removing Gel Columns (Thermo Fisher Scientific) before the isolation ...
-
bioRxiv - Cell Biology 2024Quote: ... Samples were processed for gel electrophoresis on 4-12% NuPAGE Bis-Tris gels (Invitrogen) in either MES or MOPS NuPAGE running buffer (Invitrogen) ...
-
bioRxiv - Biophysics 2021Quote: ... The cylindrical shape was given during desalting the mixed solutions using a Slide-a-Lyzer® dialysis cassette (Thermo Fisher Scientific, France) with molecular weight cut-off equal to 10000 g mol−1 under a magnetic field of 0.3 T (Supporting Information S3).51
-
bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% FBS that had been dialyzed for 1 h in excess PBS with Slide-A-Lyzer dialysis cassette (Thermo Fisher Scientific). Cells were then pulse-labeled for 1 h by adding 100 μCi/ml 35S-methionine plus 35S-cysteine (American Radiolabeled Chemicals) ...
-
bioRxiv - Neuroscience 2021Quote: ... a transcription factor neurogenin-2 in a doxycycline inducible cassette was stably integrated into the AAVS1 safe harbour locus of iPSCs cultured in Essential 8 media (ThermoFisher Scientific, A1517001). The expression of neurogenin-2 in the iPSCs was induced for three days using doxycycline (Sigma-Aldrich ...
-
bioRxiv - Immunology 2019Quote: ... DFBS was made dialyzing against 100 volumes of distilled water (five changes in three days) using Slide-ALyzer™ G2 dialysis cassettes with cut-through MW size 2K (ThermoFisher Scientific) at 4°C.
-
bioRxiv - Biochemistry 2020Quote: ... unreacted PTAD was removed by dialysis overnight in 1 L of 500 mM Tris (pH 7.4) with Slide-A-Lyzer™ G2 dialysis cassettes (10 K MWCO, Fisher Scientific, #87729). CD spectra were averaged from two or three replicates.
-
bioRxiv - Cancer Biology 2022Quote: ... We verified the integrity of the antibiotic gene cassette by sequence analysis using ABI Prism dye terminator cycle sequencing (Thermo Fisher Scientific). Erythromycin (5 µg ml ...
-
bioRxiv - Neuroscience 2020Quote: ... Fractions with refractive index from 1.370 to 1.374 were dialyzed in MWCO 7000 Slide-A-Lyzer cassettes (Thermo Fisher Scientific, Waltham, Massachusetts) overnight at 4°C ...
-
bioRxiv - Neuroscience 2020Quote: ... Fractions with refractive index from 1.370 to 1.374 were dialyzed in MWCO 7000 Slide-A-Lyzer cassettes (Thermo Fisher Scientific, Waltham, Massachusetts) overnight at 4°C ...
-
bioRxiv - Plant Biology 2021Quote: ... The cDNA fragments were then cloned into the modified pER8 plasmid containing a Gateway cassette (pER8-GW)52 using the Gateway LR Clonase □ (Thermo Fisher Scientific). For plant transformation ...
-
bioRxiv - Molecular Biology 2022Quote: ... The AutoGrid was taken out of the shuttle and placed into the autoloader cassette which was loaded into the NanoCab (Thermo Fisher Scientific) and transferred to the electron cryo microscope.
-
bioRxiv - Synthetic Biology 2019Quote: ... Elution fractions 2-4 were combined and dialyzed overnight in 500 mL of DB1 at 4 °C using 10K MWCO Slide-A-Lyzer™ Dialysis Cassettes (Thermo Fisher). The protein was dialyzed again in 500 mL DB2 for 6 hours ...
-
bioRxiv - Synthetic Biology 2020Quote: 90 μL CFE semi-continuous reactions were performed using 3.5 kDa MWCO 96-well plate dialysis cassettes (Thermo Scientific, Rockford, IL, USA) in 2 mL microcentrifuge tubes with 1.4 mL dialysis buffer solution ...
-
bioRxiv - Genomics 2021Quote: ... 4*105 TX1072 cells cultured in SL medium were reverse transfected with 250 ng pX330-PK45/46 and 750 ng of the pA cassette-containing donor plasmid (SP292) with Lipofectamine 3000 reagent using 1 µl P3000 reagent and 6 µl Lipofectamine 3000 (Thermo Fisher Scientific) following the manufacturer’s guidelines ...
-
bioRxiv - Plant Biology 2020Quote: ... Gateway cassette and the terminator) were PCR amplified and cloned into a pJET vector using a CloneJET PCR Cloning Kit (ThermoFisher Scientific, UK). All primers (Table S6 ...
-
bioRxiv - Microbiology 2020Quote: ... recombination cassettes were generated from the pEPKanS template by polymerase chain reaction (PCR) with Phusion High Fidelity DNA polymerase (Thermo Fisher Scientific) using long oligonucleotides (Ultramers ...
-
bioRxiv - Immunology 2020Quote: ... Product containing fractions were pooled and desalted by dialysis (Slide-A-Lyzer™ dialysis cassettes, MWCO 20 kDa, Thermo Fisher Scientific, USA) against desalting buffer (8 mM Na-phosphate buffer pH 7 ...
-
bioRxiv - Cell Biology 2021Quote: ... they were dialyzed overnight using Slide-A-Lyzer G2 dialysis cassettes with a molecular weight cutoff of 10 K (Thermo Fisher Scientific). The size was determined by DLS measurements using a Zetasizer Nano ZS (Malvern Instruments Ltd. ...
-
bioRxiv - Microbiology 2020Quote: ... Tryptophan-depleted media was supplemented as described for DMEM with the exception that the FBS was dialyzed in a 10 kDa MWCO dialysis cassette (Thermo Fisher Scientific) for 16-20 hrs at 4° C in 4 L of PBS ...
-
bioRxiv - Biochemistry 2022Quote: Multi-specimen cassettes with grids were directly transferred from the FIB/SEM to the transmission electron microscope via an Autoloader (Thermo Fisher Scientific). Tomography data were acquired with a Titan Krios (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2022Quote: Autogrid clipped TEM grids were loaded into a cassette and loaded into the PFIB’s multispecimen cassette which was then subsequently loaded onto the stage using the robotic sample delivery device (termed Autoloader) (Thermo Fisher Scientific). The SEM was used to screen the grids to ensure suitability for lamella preparation (Fig ...
-
bioRxiv - Genomics 2022Quote: ... Cytospin preparations with 100 μl aliquots were prepared in cytocentrifuge cassettes (Thermo Scientific Cytospin 4 centrifuge, Thermo Fisher Scientific, Waltham, Massachusetts, US). Slides were stained with May-GrÜnewald-Giemsa and evaluated by a clinical pathologist ...
-
bioRxiv - Synthetic Biology 2022Quote: ... the kanamycin (KanMx) antibiotic resistance cassette was amplified by PCR using a Phusion Flash high-fidelity PCR master mix (Thermo Scientific, USA) and 70-nt primers for direct homologous recombination on the BAR1 locus ...
-
bioRxiv - Molecular Biology 2022Quote: ... Peak fractions were pooled and dialyzed three times against Mili-Q dH2O using slide-a-lyzer dialysis cassettes with a 2 kDa cut-off (Thermo Fisher Scientific). The desalted sample was lyophilized and resuspended in 100 μl Mili-Q dH2O ...
-
bioRxiv - Microbiology 2022Quote: ... the band at the junction of the 1.2 g/L and 1.4 g/L CsCl was extracted and transferred to 3-12 mL Slide-A-Lyzer dialysis cassettes with a molecular weight cutoff (MWCO) of 10K (Thermo Fisher Scientific). The membranes were placed in 1 × Dulbecco’s phosphate-buffered saline (DPBS ...
-
bioRxiv - Microbiology 2022Quote: ... They were then pooled and transferred to 3-12 mL Slide-A-Lyzer dialysis cassettes with a MWCO of 10K (Thermo Fisher Scientific). The membranes were placed in 1 × DPBS with Mg and Ca salts (Gibco) ...
-
bioRxiv - Biochemistry 2023Quote: ... folding occured at 4 °C upon dialyses against the following solutions with Slide-A-Lyzer™ dialysis cassettes 3500 MWCO from Thermo Scientific: 3 M GndHCl ...
-
bioRxiv - Cancer Biology 2022Quote: ... pCX4- puro was converted into a Gateway™ Destination vector (pCX4-DEST-Puro) by inserting the Gateway™ Reading Frame Cassette A (ThermoFisher) into the multiple cloning site using EcoRI and XhoI restriction sites ...
-
bioRxiv - Microbiology 2023Quote: ... the recombination cassette was generated from the pEPKanS template by polymerase chain reaction (PCR) with Phusion High Fidelity DNA polymerase (Thermo Fisher Scientific) using long oligonucleotides (Ultramers ...
-
bioRxiv - Molecular Biology 2023Quote: The supernatant containing the proteins was transferred to a a Slide-A-Lyzer™ G2 Dialysis Cassette (3.5K) (Thermo Fisher Scientific; 87724) and dialyzed against 3 L of dialysis buffer (20 mM Tris/Cl (pH=7.3) ...
-
bioRxiv - Microbiology 2023Quote: ... The cell lysate was dialyzed overnight at 4°C against PBS using a Slide-A-Lyser mini dialysis cassette (MWCO 3500, Thermo Fisher Scientific). For the input ...
-
bioRxiv - Microbiology 2023Quote: ... The TEV cleavage reaction was incubated at 4°C for 16 hours in a 30 mL 10 kDa molecular weight cutoff dialysis cassette (ThermoFisher Scientific, 66830) dialyzing against 2 L TEV reaction buffer ...
-
bioRxiv - Biophysics 2023Quote: ... according to Bahrenburg et al.,56 followed by dialysis carried out in a Slider-A-Lyzer dialysis cassette (Thermo Scientific, Rockford, USA) against 10 mM histidine ...
-
bioRxiv - Immunology 2023Quote: ... and dialyzed against phosphate buffered saline (PBS) at 4°C in 10,000 MW cutoff Slide-A-Lyzer dialysis cassettes (ThermoFisher, Pittsburgh, PA, USA) prior to use in mice ...
-
bioRxiv - Bioengineering 2023Quote: ... LNPs were dialyzed against 1x PBS for 4 hours using Slide-A-Lyzer™ G2 Dialysis Cassettes with a 10 kDa molecular weight cut-off (87730, Thermo Fisher). Formulations were stored at 4°C until use.
-
bioRxiv - Bioengineering 2024Quote: ... followed by overnight dialysis against sterile PBS at 4°C in 10 kDa Slide-a-Lyzer G2 cassettes (Thermo Fisher, MA, USA). The batch size of liposomes was 1.2 L in total ...
-
bioRxiv - Bioengineering 2024Quote: ... followed by overnight dialysis against sterile PBS at 4°C in 10 kDa Slide-a-Lyzer G2 cassettes (Thermo Fisher, MA, USA).
-
bioRxiv - Molecular Biology 2021Quote: ... 1.5 mm gels (Thermo Fisher) and resolved ...
-
bioRxiv - Biochemistry 2021Quote: Tris-Acetate gels (Thermofisher Scientific)
-
bioRxiv - Biochemistry 2020Quote: ... Bis-Tris gels (Thermo Fisher). Protein bands were transferred onto Trans-Blot Turbo Midi 0.2 µm PVDF membranes (BioRad ...
-
bioRxiv - Cell Biology 2021Quote: ... gel with SYBRSafe (Life Technologies) in 1 x TBE at +4C ...