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Citations for Thermo Fisher :
8001 - 8050 of 10000+ citations for Biotin Z Antibody Internalization Kit rabbit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... Ten 150mm plates of confluent cells expressing each construct were grown overnight in media with a final concentration of 50 uM D-Biotin (Life technologies, B-20656).
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bioRxiv - Developmental Biology 2021Quote: ... the sections were incubated with a secondary antibody conjugated with a biotin or fluorescence label (for immunofluorescence) or biotin for immunohistochemistry (for CD31, PEG10 and CD45, 1:500 goat anti-rat IgG conjugated with Cy3 (Thermo Fisher Scientific, A10522); for Cytokeratin ...
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bioRxiv - Immunology 2021Quote: ... The messenger RNA (mRNA) was isolated from total RNA by Dynabeads M-270 streptavidin with biotin-oligo (dT)25 (Thermo Fisher Scientific, USA). First- and second-strand cDNA synthesis was performed by Superscript reverse transcriptase IV (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The biotin-marked ligation products were then enriched by affinity capture with streptavidin magnetic beads (DynaBeads MyOne Streptavidin C1; Invitrogen, Thermo Fisher Scientific). Subsequently ...
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bioRxiv - Immunology 2022Quote: ... A mock depletion (undepleted control) was performed for each serum by adding 0.5 mg Biotin-saturated MyOne™ Streptavidin T1 Dynabeads™ (ThermoFisher, Cat.no. 65601) per 100 µL serum ...
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bioRxiv - Cell Biology 2024Quote: ... 2.0µM Template Switching Oligo (TSO) (5′-Biotin-AGAGACAGATTGCGCAATGNNNNNNNNrGrGrG-3′; IDT) and 2U µl−1 of Maxima H Minus reverse transcriptase (Thermo Fisher Scientific, #EP0752). Plates were quickly centrifuged before incubation at 42°C for 90min ...
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bioRxiv - Neuroscience 2024Quote: Cerebellar acute slices were washed in 37°C pre-warmed ACSF (see above) for 30 min before incubation in ACSF containing 1 mg/ml EZ-Link™ Sulfo-NHS-SS-Biotin (Thermo Fisher Sci) for 1h at 4°C ...
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bioRxiv - Microbiology 2023Quote: Human LDL-c and HDL-c were biotinylated using Sulfo-NHS-LC-biotin and purified using a 10Kd-cut off desalting column (ThermoFisher, Waltham, MA, USA). The biotin-LDL-c (2 μg ...
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bioRxiv - Immunology 2023Quote: ... The IgH and γ-satellite probes (which highlight heterochromatin) were respectively labelled with dCTP-biotin or dUTP-digoxigenin (Invitrogen REF#19518018 or #11573152910) and dUTP-Alexa Fluor 488 (Invitrogen REF#C11397) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Soluble membrane proteins were isolated from homogenized and sonicated Expi 293F cells followed by biotinylation with Sulfo-NHC-SS-Biotin (Thermo Fisher Scientific, 21331) and stored in PBS at −80 °C ...
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bioRxiv - Biochemistry 2023Quote: ... The GMPCPP-stabilized microtubules were immobilized on the surface of a cover glass using the biotin-NeutrAvidin protein links (Thermo Fisher Scientific, 31000). GFP-MCAK was then added into the flow cell in the imaging buffer ...
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bioRxiv - Synthetic Biology 2023Quote: ... we added all four nucleotides at a final concentration of 1 µM (Biotin-7-dATP, Jena Biosciences, and dC-Atto647N or dU-Atto488, Jena Biosciences with respective pair of unmodified nucleotides, Thermo Scientific): [Biotin-7-dATP ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 ng of ribo-depleted RNA was biotin-clicked to label-probed bases followed by RNA fragmentation for 40 s using RNA fragmentation reagents (Thermo Fisher Scientific, AM8740). Fragmented RNA was end-repaired ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were washed in cold PBS and incubated for 30 min with EZ-Link™ Sulfo–NHS–LC–biotin (1 mg/mL; ThermoFisher Scientific, 21335) in cold PBS (pH 8) ...
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bioRxiv - Immunology 2024Quote: ... were produced and purified as described.73 Chemical biotinylation of lectin ECD was performed using EZ-Link™ Sulfo-NHS-LC-Biotin (Thermo Scientific #21335) according to manufacturer instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... which was labeled using an Alexa FluorTM 488 Antibody Labeling Kit (Molecular Probes/Thermo Fisher Scientific, Eugene, OR, USA)
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bioRxiv - Microbiology 2020Quote: Polyclonal anti-CD55 antibody and control IgG were digested using the Pierce™ Fab preparation kit (Thermo Fisher Scientific). The resulting Fab fragments were quantified on a spectrophotometer ...
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bioRxiv - Evolutionary Biology 2019Quote: Affinity purified antibodies were coupled to agarose A/G using the Pierce Crosslink CoIP Kit (Thermo Scientific Cat #26147). A control IP was performed using rabbit IgG (I5006 ...
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bioRxiv - Cell Biology 2021Quote: Bovine photoreceptor outer segments (POS) were labeled with FITC using the Pierce FITC Antibody Labeling Kit (Thermo Fisher Scientific). Adherent RPE cells were treated with ~ 5 POS per cell and 1 x 106 POS were applied per RPE organoid ...
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bioRxiv - Microbiology 2021Quote: ... Total antibody levels in the circulation were then determined in sera from sham treated controls (naïve) for each age group and the hyperimmune standard serum using antibody quantification kits from Invitrogen for IgG (88-50400) ...
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bioRxiv - Cell Biology 2020Quote: ... Immunological detection was performed using horseradish peroxidase secondary antibodies and ECL or Femto ECL kit (Invitrogen, Carlsbad, CA, USA). Images were acquired using Fusion Capt Advance FX7 (Vilber ...
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bioRxiv - Neuroscience 2020Quote: The quantitation of soluble human Aβ1-42 in hippocampus extracts was determined with a sandwich antibody ELISA kit (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Cells were then subsequently stained with an antibody cocktail containing LIVE/DEAD Fixable Dead Cell Stain Kit (Thermo Fisher), and surface stained with the antibodies listed in S2-S4 Tables for 30 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: The following reagents and antibodies were used: Live/dead fixable dead cell stain kits (blue and violet, Molecular Probes), TruStain FcX anti-mouse CD16/CD32 (93 ...
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bioRxiv - Molecular Biology 2023Quote: ... The isotypes of antibodies generated from hybridoma clones were determined using Pierce mouse Ig isotyping kit (Thermo Fisher 26178). The selected hybridomas produced IgG.
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bioRxiv - Neuroscience 2023Quote: ... followed by immunoblots using NaV1.7 antibody and silver stain on an additional SDS-PAGE gel using Pierce silver stain kit (ThermoFisher). The stained gel regions of interests were excised ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with fluorescent dye-conjugated antibodies (see Table I) and fixed (Foxp3 fixation/permeabilization Kit, Invitrogen). Intracellular stains (i.e. ...
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bioRxiv - Biophysics 2019Quote: ... Rabbit anti GFAP antibody (kindly supplied by A. Triller, ENS Paris, 1:250) and Alexa 488 conjugated goat anti-rabbit (Thermo Fisher Scientific, Waltham, USA, 1:250), for neurons ...
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bioRxiv - Neuroscience 2021Quote: ... sections were washed 3 times for 10 minutes per wash in PBS before incubation in secondary antibody Alexafluor 488 Goat-anti-rabbit (Thermo Fisher 150077; [1:500]; Waltham, MA) diluted in probing solution for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were washed with PBS and incubated in the appropriate fluorescent secondary antibodies: Alexa 568 donkey anti-rabbit (Thermo Fisher Scientific, 1:500 dilution in PBS), Alexa 488 donkey anti-goat (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... kidney sections were incubated with Alexa Fluor 488 conjugated goat anti-rabbit and Alexa Fluor 596 conjugated goat anti-Guinea pig IgG secondary antibodies (Thermo Fisher, Rockford, IL, USA, 1:300). Images were visualized using AxioCam cameras (Zeiss ...
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bioRxiv - Bioengineering 2021Quote: ... and incubated with secondary antibodies including DAPI (0.1 µg/ml) for 1 h at RT in the dark: Alexa Fluor 488 (Thermo Fisher Scientific, 1:300, sheep/mouse/rabbit), Alexa Fluor 568 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The wells were washed 3 times with 1 mL PBS 1X and covered with a mixture containing secondary goat anti-rabbit-Alexa488 antibodies (Invitrogen Molecular Probe 2mg.mL-1, 1/300e) and DAPI (1/100e ...
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bioRxiv - Cell Biology 2020Quote: ... sections were washed 2 times for 8 minutes in DPBS containing 0.3% Triton X100 and then incubated for 1 hour in blocking solution containing the secondary antibody (1/200, Invitrogen P10994 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody ...
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bioRxiv - Neuroscience 2022Quote: ... three times and incubated with horseradish peroxidase-conjugated secondary antibodies (Mouse, Cell Signaling, cat #7076S; Rabbit, Cell Signaling, cat #7074S; Rat, Cell Signaling, cat #7077S; Sheep, Thermo Fisher, cat #A16041) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... 1:2000 in 0.1% bovine serum albumin (BSA) in PBS) followed by goat anti-rabbit Alexa Fluor 488 antibody (Invitrogen, 1:2000 in 0.1% BSA in PBS). Plates were scanned on the Amersham Typhoon Biomolecular Imager (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed three times in PBS and incubated with Alexa-fluor-conjugated secondary antibodies (goat anti-guinea pig, A-11073, Invitrogen, 1:5,000; goat anti-rabbit (31460) and goat anti-mouse (31430) ...
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bioRxiv - Microbiology 2021Quote: ... followed by an incubation with Alexa Fluor 594 anti-mouse and Alexa Fluor 488 anti-rabbit antibodies (Molecular Probes, 1:500 in PBS/0.01% Triton-X) for 45 minutes in the dark ...
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bioRxiv - Immunology 2022Quote: ... The cells were washed four times in PBS and incubated with goat anti-rabbit antibody conjugated with AlexaFluor594 for 1 hour at room temperature (Invitrogen; 1:200 dilution in blocking reagent). 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2019Quote: ... the cells were incubated with goat anti-rabbit or anti-mouse IgG antibody conjugated with Alexa Fluor 488 dye or Alexa Fluor 568 dye (Life Technologies; 1000x dilution in blocking solution) for 60 min at room temperature ...
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bioRxiv - Zoology 2021Quote: ... sections were incubated successively in PBST with 1) an anti-phosphorylated ribosomal protein S6 (pS6) rabbit polyclonal antibody (Ser244, Ser247 anti-pS6, 44-923G, Thermo Fisher Scientific, MA, USA; 1:5000) that was used in mouse OSNs as validated in Sharma et al ...
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bioRxiv - Developmental Biology 2021Quote: ... The primary antibodies were visualized with respective secondary goat-anti-rabbit and goat-anti- mouse antibodies conjugated with Alexa-488 (1:1000, Molecular Probes, USA; A-11008; goat; polyclonal) and Alexa-633 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1% Tween-20 for 10 min and incubated with primary antibody (1:500 mouse α8-oxoG, QED Bioscience; 1:5000 rabbit αGFP, ThermoFisher, 1:5000 mouse αHA, Covance) overnight at 4°C in a humidity chamber ...
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bioRxiv - Microbiology 2021Quote: ... Triton-X-100 and 5% (v/v) normal goat sera) for 30 min prior to staining with a rabbit anti-HA polyclonal antibody (Invitrogen, diluted 1:100 in blocking buffer) for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed 5x and incubated in 2.5 μg/mL of the corresponding secondary antibody conjugated with Alexa Fluor 647 for 30 min at room temperature (Invitrogen, anti-mouse A21237, anti-rabbit A21246). When indicated ...
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bioRxiv - Cell Biology 2022Quote: ... respectively) and the fluorescent goat anti-rabbit Alexa-488 and anti-mouse Alexa-594 secondary antibodies were from Thermo Fisher (A-11008 and A-21203, respectively).
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bioRxiv - Cell Biology 2022Quote: ... followed by a 1 hour incubation at room temperature with goat anti-mouse Alexa 564 and goat anti-rabbit Alexa Fluor 488 secondary antibodies (1:500; Invitrogen, A-11030 and A-11008 respectively), respectively ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were washed three times in 1xTBS and incubated in fluorescent Alexa-Fluor-labelled IgG secondary antibodies (Alexa-Fluor goat anti-rabbit 488 Invitrogen A-11008, goat anti-rat 546 Invitrogen A-11081 all 1:400 dilution) in 10% serum solution in 2% TBS-T for 2 h ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated for 1 hour at RT with a horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary antibody (1:1000; Thermo Fisher Scientific cat#31460, RRID: AB_228341) or an HRP-conjugated goat anti-mouse secondary antibody (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... 120 min) and then placed in secondary donkey anti-Rabbit Alexa Fluor 647-conjugated secondary antibody (A-21449; Thermo Fisher Scientific, MA, USA, Invitrogen; 1:200) for one week at 37℃ ...