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Citations for Thermo Fisher :
7901 - 7950 of 10000+ citations for Biotin Z Antibody Internalization Kit rabbit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: OK cells cultured on permeable supports under static or FSS conditions were washed with cold PBS and the apical surface biotinylated with 1 mg/mL EZ-Link Sulfo-NHS-SS-biotin (Thermo Scientific, 21331) in 0.5 mL TEA-buffered saline (TBS ...
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bioRxiv - Cell Biology 2024Quote: RPE1-hTERT cells were washed generously with ice-cold PBS prior to labelling with cell-impermeable 0.2 mg/mL Sulfo-NHS-SS Biotin (ThermoFisher Scientific, no. 21217) in PBS (pH 7.4) ...
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bioRxiv - Microbiology 2024Quote: ... The solution was then mixed with aniline (80 mM; sigma) followed by EZ-link alkoxyamine PEG-4-SS-biotin (40 mM; PEG = polyethylene glycol, Thermo Fisher Scientific). All 4 reagents were mixed in equal volumes ...
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bioRxiv - Cancer Biology 2024Quote: ... Pulldown of biotin-labeled DNA was initiated by separating 10 µL of 10 mg/mL Dynabeads MyOne Streptavidin T1 beads (Life Technologies, 65602) on a magnetic stand ...
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bioRxiv - Biochemistry 2023Quote: ... to promote DNA synthesis as previously described(Ray-Gallet and Almouzni 2004) except that the reaction mixed contained 3.2 µM of biotin-14-dCTP (Invitrogen, Ref 19518-0189) instead of [α32P]-dCTP ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were then placed on ice and incubated twice with 0.75 mg/ml NHS-SS-biotin in PBS (Thermo Scientific, Rockford, Illinois) for 30 min while bubbling with 95% O2 and 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then washed in TBS and incubated for 1 hour in TBS containing streptavidin-biotin-peroxidase complex (ABC 1:1,000 TBS; ThermoFisher Scientific, Product# 32020), washed again in TBS ...
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bioRxiv - Genomics 2023Quote: ... The library was split and a further 20 or 25 PCR cycles were performed using a biotin oligonucleotide (5—PCBio-TACACGACGCTCTTCCGATCT) and then cDNA was enriched using Dynabeads™ MyOne™ streptavidin T1 magnetic beads (Invitrogen). The beads were washed in 2X binding buffer (10mM Tric-HCL ph7.5 ...
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bioRxiv - Genomics 2023Quote: ... The library was split and a further 20 or 25 PCR cycles were performed using a biotin oligonucleotide (5-PCBio-CTACACGACGCTCTTCCGATCT) and then cDNA was enriched using Dynabeads™ MyOne™ streptavidin T1 magnetic beads (Invitrogen). The beads were washed in 2X binding buffer (10mM Tric-HCL ph7.5 ...
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bioRxiv - Biophysics 2023Quote: ... Surfaces were passivated by incubating with BSA-Biotin (1 mg/mL in 1× TE with 50 mM NaCl, Thermo Fisher Scientific, USA) and subsequent washing with 1× PBS buffer three times ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was washed in three 10-min PBS exchanges and underwent tyramine signal amplification by treating with PBT comprising 0.005% hydrogen peroxide and 0.5% borate-buffered biotinylated (Sulfo-NHS-LC biotin; 21335, Thermo Fisher Scientific, Waltham, MA) tyramine (T90344 ...
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bioRxiv - Immunology 2023Quote: ... using a Superdex S75 10/300 column in 1XPBS and chemically biotinylated using EZ-Link™ Sulfo-NHS-Biotin (Thermo Fisher Scientific) following manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylation of Htz1(V126C) was carried out using N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)propionamide (HPDP-Biotin) (Thermo Fisher cat. # 21341), which has a pyridyl disulfide moiety ...
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bioRxiv - Cell Biology 2023Quote: ... followed 1 µg/ml donkey anti-mouse secondary and 2 µg/ml streptavidin-Alexafluor-488 reagent (to visualise the biotin, Thermo Fisher Scientific). Nuclei were counterstained with 10 µg/ml DAPI (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... with 10 μL of 10 μg/mL Biotin-BDNF (Alamone Labs, Jerusalem, Israel) and 39 μL of the quantum dot incubation buffer (Q20001, Thermo Fisher Scientific) for a minimum of 30 minutes in the dark on ice ...
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bioRxiv - Molecular Biology 2024Quote: DNA probes were biotinylated at the 3’terminal using Biotin 11-dUTP (Thermo: R0081) and terminal deoxynucleotidyl transferase TdT enzyme (Thermo scientific: EP0161) in the presence of CaCl2 containing 5X reaction buffer ...
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bioRxiv - Cell Biology 2024Quote: ... gene knock-in was achieved via electroporation of RNP complexes and various DNA donor templates (unmodified, 5’ Biotin, 5’ AmC6, or dbDNA) using the Neon Transfection System (Thermo Fisher Scientific) following the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2021Quote: ... membranes were incubated with the appropriate secondary antibody for 1 hr at room temperature: HRP-conjugated goat anti-rabbit (Thermo Fisher Scientific, cat# 31460; RRID:AB_228341) at 1:5000 dilution in 1% w/v BSA/TBST (or 5% w/v nonfat dry milk/TBST ...
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bioRxiv - Developmental Biology 2021Quote: ... The cells were washed 3 times with PBS for 5 min at room temperature and incubated with Alexa-Fluor labeled secondary antibodies (goat anti-mouse 568 or goat anti-rabbit 488; Invitrogen A11004, A11008, respectively, 1:400). In order to identify if fibronectin was an ADAMTS6 substrate ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were incubated with the corresponding secondary fluorophore-conjugated antibodies Alexa Fluor goat anti-mouse 488 and goat anti-rabbit 594 (Thermo Fisher Scientific; dilution 1:1000) in blocking solution for 45 min ...
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bioRxiv - Molecular Biology 2020Quote: ... The staining was visualized by fluorescence microscopy with fluorescent-conjugated secondary antibodies (goat anti rabbit Alexa Fluor 594, 1:400, ThermoFisher,Waltham, MA, cat. A-11034). Nuclei were counterstained with Hoechst 33348 dye (1:1000) ...
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bioRxiv - Genomics 2020Quote: ... at 1:500 dilution followed by incubation for 1h (dilution 1:500) with secondary antibodies (Alexa 488 conjugated anti-mouse IgG and Alexa 568 conjugated anti-rabbit IgG (Thermo Fisher Scientific, A11029 and A21428)) ...
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bioRxiv - Developmental Biology 2020Quote: ... and incubated for 30 minutes at room temperature with a 1:1000 dilution of donkey anti-rabbit IgG (H+L) secondary antibody conjugated to Alexa Fluor® 647 (ThermoFisher-Invitrogen, catalog A-31573) in blocking solution ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were probed with secondary antibodies Alexa Fluor 488 anti-rabbit IgG or Alexa Fluor 488 anti-mouse IgG or (all Thermo Fisher Scientific, diluted 1:500) for 2 h at room temperature under minimal light conditions ...
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bioRxiv - Immunology 2019Quote: ... Samples were washed thrice with blocking buffer and incubated for 2 hr with anti-rat Alexa Fluor® 488- and anti-rabbit Alexa Fluor® 594-conjugated secondary antibodies (ThermoFisher Scientific, 1:400). After washing ...
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bioRxiv - Cell Biology 2021Quote: ... Later the cells were incubated with the secondary anti-Rabbit IgG antibody conjugated with Alexa Fluor 488 (Life Technologies, Cat. no.: A11008; Lot no.: 1735088) at the dilution of 1:200 in 1% BSA made in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... in Tris-buffered PBS/ 0.1 % Tween 20 for 1 h at room temperature they were incubated overnight at 4 °C with the following antibodies: rabbit polyclonal anti-2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase; 49 kDa; 1:1000; Thermo Fisher Scientific, Waltham, MA, USA), mouse monoclonal anti-myelin-associated glycoprotein (MAG ...
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bioRxiv - Bioengineering 2020Quote: ... The sections were blocked with 5% BSA in PBS prior to incubation overnight at 4°C with primary antibody (rabbit anti-connexin-36, Life Technologies 36-4600, 1:50) in 1% BSA ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibodies used for all stages were Alexa Fluor-conjugated (AF488, AF594) and specific to rabbit or mouse (1:1000, Invitrogen, Cat# A11001, A11005, A11008, A11012). 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then washed four times in wash buffer for 10 min each and stained with secondary antibodies (Alexa-Fluor 488 goat anti-rabbit and Alexa-Fluor 568 goat anti-mouse (Life Technologies, A-11008 and A11004), both 1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... which was followed by the incubation with the secondary antibodies (anti-rabbit, anti-mouse and anti-guinea pig IgG conjugated to Alexa 488 or Alexa 568, Thermo Fisher Scientific, 1:500 dilution) for 2 hours at RT ...
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bioRxiv - Microbiology 2021Quote: ... Alexa-Fluor® 647-labelled rabbit anti β-tubulin IV (Cell Signalling Technology, 9F3) and Alexa-Fluor® 594-labelled mouse antibody against ZO-1 (Thermo Fisher Scientific, 1A12) were used to visualize cilia and tight junctions ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated with secondary antibodies: Alexa Fluor 594 goat anti-rabbit or Alexa Fluor 488 goat anti-mouse IgG(1:1000, Life Technologies Corporation, Carlsbad, CA, USA) for 1 h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... For GFP immunohistochemistry sections were incubated in anti-GFP antibody (dilution 1:20 000, polyclonal rabbit-anti GFP, A6455, Life Technologies, Grand Island, NPY, USA) for 48 hours at 4C ...
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bioRxiv - Cell Biology 2021Quote: ... Raw Ct data resulting from specific antibodies-immunoprecipitated chromatin were normalized to negative isotypic control rabbit IgG (cat. 492024-Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the membranes were incubated 1 h at room temperature with the peroxidase-labelled polyclonal secondary anti-rabbit antibody (#31430; 1:10000, Thermo Fisher Scientific, Waltham, MA, USA). After washing ...
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bioRxiv - Neuroscience 2019Quote: ... the neurons were incubated with goat anti-rabbit IgG antibody conjugated with Alexa Fluor 488 dye (A-11070, Life Technologies; 1000x dilution in blocking solution), and anti-mouse IgG antibody conjugated with Alexa Fluor 568 dye (A-11019 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were washed four times with PBST (PBS with 0.1% Triton X-100) and then treated with an anti-mouse antibody (Thermofisher, Rabbit anti-Mouse IgG (H+L) Secondary Antibody ...
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bioRxiv - Neuroscience 2020Quote: ... the slices were washed with PBST (PBS with 0.05% triton X-100) and incubated with anti-mouse antibody (Thermofisher, Rabbit anti-Mouse IgG (H+L) Secondary Antibody ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated at room temperature for 90 min in a cocktail of the following secondary antibodies: goat anti-rabbit Alexa Fluor 555 (1:500; Molecular Probes, Invitrogen, Cat# A-21429, RRID:AB_2535850), donkey anti-mouse Alexa Fluor 647 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated for 48 hours at 4°C in primary rabbit polyclonal anti-GFP antibody (A-6455; dilution 1: 80 000; Life Technologies, Grand Island, NY, USA). Sections incubated in biotinylated goat anti-rabbit IgG (1:500 BA-1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... The sections were incubated with Alexa Fluor 488 donkey anti-rabbit IgG and Alexa Fluor 633 goat anti-mouse IgG secondary antibodies for 1 h (1:200; Molecular Probes by Life Technologies, Waltham, MA, USA). Control slides were incubated with the secondary antibody alone ...
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bioRxiv - Cell Biology 2019Quote: ... the samples were incubated with an Alexa Fluor®594 conjugate goat polyclonal anti-rabbit IgG secondary antibody (A-11012, Life Technologies, Saint Aubin, France) diluted at 1:100 in 1 % (v/v ...
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bioRxiv - Cell Biology 2019Quote: ... the samples were incubated with an Alexa Fluor®594 conjugate goat polyclonal anti-rabbit IgG secondary antibody (A-11012, Life Technologies, Saint Aubin, France) diluted at 1:100 in 1 % (v/v ...
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bioRxiv - Cell Biology 2019Quote: ... The cells were incubated for 90 min with the Alexa Fluor® 594 conjugate goat polyclonal anti-rabbit IgG secondary antibody (A-11012, Life Technologies, Saint Aubin, France) diluted at 1:100 in 1 % (v/v ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated with secondary antibodies: Alexa Fluor 594 goat anti-rabbit or Alexa Fluor 488 goat anti-mouse IgG (1:1000, Life Technologies Corporation, Carlsbad, CA, USA) for 1 h at RT ...
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bioRxiv - Physiology 2019Quote: ... sections were incubated for 1 h at room temperature with anti-rabbit Alexa Fluor A488 antibody (A21206, 1:1000, Thermo Fisher Scientific, Waltham, MA, USA). Slides were washed with PBS and coverslipped with DAPI Fluoromount-G (0100-20 ...
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bioRxiv - Neuroscience 2019Quote: ... sections were washed then labeled with secondary antibodies (anti-goat AlexaFluor 568 cat. No. A31573, 1:400; or anti-rabbit AlexaFluor 647, 1:400; Thermo Fisher Scientific, Waltham, MA, USA) as well as FITC-Avidin (cat ...
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bioRxiv - Genomics 2021Quote: ... Texas USA).Secondary goat anti-rabbit antibody (1:10000 dilution) was added (1hour RT) and the membranes washed before Horse Radish Peroxidase substrate (Thermo Fisher Scientific, Waltham, Massachusetts, US) added for imaging ...
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bioRxiv - Physiology 2020Quote: ... re-blocked and incubated in secondary antibodies (Goat anti-rabbit 555 [Dylight], Donkey anti-mouse [Thermofisher Scientific], Goat anti-chick 647 [Thermofisher scientific] all at 1:500) o/n at 4C ...