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Citations for Thermo Fisher :
7951 - 8000 of 10000+ citations for Biotin Z Antibody Internalization Kit rabbit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 1 hour respectively with the secondary antibodies goat anti-rabbit IgG (H+L) conjugated to Alexa fluor 594 (Molecular Probes A-11012, 1:400); goat anti-mouse IgM conjugated to Alexa fluor 594 (Invitrogen ...
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bioRxiv - Genomics 2021Quote: ... sections were incubated with primary antibody (rabbit anti-LYZ, 1:1000 dilution in PBS with 1% (w/v) BSA) (Invitrogen, Carlsbad, CA, cat. PA5-16668) overnight at 4° C followed by goat anti-rabbit Alexa fluor 594 secondary antibody (1:1000 in PBS with 1% (w/v ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated at room temperature for 1 h with secondary antibodies Alexa Fluor 568 goat anti-mouse IgG and Alexa Fluor 488 goat anti-rabbit IgG (1:500; Invitrogen Life Technologies, Rockford, IL, USA), counterstained with Hoechst 33258 (1:1,500 ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed several times in PBS and incubated with carrier solution containing the secondary antibody (goat anti-rabbit 488, 1:750, Thermo Fisher Scientific Cat# A-11011) and in case of the midbrain sections Streptavidin AlexaFluor 408 (1:750 ...
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bioRxiv - Cell Biology 2022Quote: ... Both secondary antibodies (Alexa Fluor 568 donkey anti mouse, Thermo Fisher Scientific; A10042 and Alexa Fluor 488 donkey anti rabbit, Thermo Fisher Scientific; A-21202) were diluted 1:400 and incubated for 2hrs at RT on a shaking plate ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Slides were subsequently incubated with the following goat secondary antibodies for 1 hr at room temperature: anti-rabbit 568 (Thermo Fisher Scientific, A11036; 1:1000), anti-mouse 488 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... samples were washed in TBS-T and incubated with secondary antibodies at room temperature for one hour (AlexaFluor 594 goat anti-rabbit IgG (H+L) (Invitrogen, Ireland, A-11012, 1:500), Cy®5 goat anti-mouse IgG (H+L ...
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bioRxiv - Neuroscience 2023Quote: ... slides were washed and incubated 3 h with secondary antibodies: Alexa Fluor 488 (Anti-rabbit, 1:1000, Invitrogen, Themo Fisher Scientific, Slangerup Denmark, #A11008, AB_143165), Cy3 (monoclonal donkey anti-rabbit 1:600 ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies used were goat anti-mouse or goat anti-rabbit polyclonal IgG (H+L) HRP conjugates (Thermo Fisher Scientific; #31430 or #32460; 1:10,000).
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bioRxiv - Neuroscience 2022Quote: ... sections were again washed in 0.01M PBS and incubated for 1 h at room temperature with the secondary conjugated antibody (1:1000, Invitrogen: goat anti-rabbit Alexa Fluor 488). The sections were washed in 0.01M phosphate buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Next day cells were washed with PBS containing 0.1% Tween- 20 and then incubated by secondary goat rabbit Alexa Fluor 488 antibody (Thermofisher, 1:1000 in PBS/4%BSA) at room temperature in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated for 1 h at room temperature with Alexa Fluor 488-conjugated donkey anti-rabbit secondary antibody (1:200; Invitrogen, A-21206, Waltham, MA, USA). The Gi-DREADD or stained brain sections were mounted on resin-coated slides and dried slightly ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated overnight in the primary antibodies diluted in the blocking solution then incubated sequentially with the secondary antibodies (donkey anti-rabbit Alexa Fluor 594 and donkey anti-goat Alexa Fluor 488, both Life technologies, conc. both 1:1000). Finally ...
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bioRxiv - Molecular Biology 2022Quote: ... and a 1-hour incubation at room temperature with secondary antibodies (Dylight 488 anti-mouse, KPL, ref 072-03-18-06, 1/200; anti-rabbit AlexaFluor 488, Life Technologies, ref A21441, 1/200), slides were mounted in Vectashield mounting medium (Vector ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were washed with PBS three times and then coated with Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (Thermo Scientific, A-11034, 1:200) for 1 h at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... The following secondary antibodies were used: goat anti-rabbit IgG (H+L) conjugated with Alexa Fluor 555 (A-21428; Thermo Fisher Scientific, Waltham, MA, USA), goat anti-rat IgG (H+L ...
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bioRxiv - Microbiology 2024Quote: ... cells were washed to remove excess antibody and incubated in goat anti-mouse secondary antibody AF568 (Thermo Fischer Scientific, A-11004, 1:500) and goat anti-rabbit AF488 (Life technologies, A-11008, 1:500) for one hour ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... incubation was performed with the secondary antibody made in Alexa FluorTM 568 donkey anti-rabbit (Invitrogen®, Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA), at a dilution of 1 ...
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bioRxiv - Neuroscience 2023Quote: ... followed by an incubation in secondary fluorescent antibodies (1:500, Alexa Goat anti-Mouse IgG2b, A-21141, Alexa Goat anti-Rabbit IgG, A32733) and DAPI (Thermo Fisher Scientific; 62248, 1:500) in 0.1x PBS with 0.1% Triton X100 for 1 hour at 22-24°C ...
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bioRxiv - Bioengineering 2023Quote: ... Slides were then washed with 1x TBS twice for 5 minutes each and incubated with either Alexa Fluor 647 donkey anti-rabbit secondary antibody (A31573, Thermo Fisher Scientific, Waltham, MA, USA) or Alexa Fluor 647 anti-rat secondary antibody (A-21247 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed in PBS and incubated in donkey anti-rabbit Alexa Fluor 488 secondary antibody (1:500, Thermo Fisher Scientific, #A11055, Waltham, MA) in PBS for 1hr at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... for 1 hour and then detected with 1:10000 dilution goat anti-Rabbit IgG Secondary Antibody conjugated with Alexa Fluor™ 488 (ThermoFisher Scientific, Cat #A-11008). Tissue was counterstained with DAPI ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... followed by the addition of secondary antibodies in blocking buffer (goat-anti-rabbit-IgG-AlexaFluor488 [ThermoFisher Scientific #A11034] and goat-anti-mouse-IgG-AlexaFluor568 [ThermoFisher Scientific #A11031] both 1:200) and Hoechst 33342 (2 ug/mL from 10 mg/mL stock ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated with secondary antibodies conjugated to an Alexa-488 fluorophore (1:500 dilution of goat anti rabbit Alexa Fluor-488; Thermo Fisher Scientific, cat. no. A32731), for 2 h at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... RT) followed by a 2-hour incubation in secondary antibody: goat anti-rabbit IgG Alexa Fluor 488 (cat. A11008 Invitrogen, 1:500 in 1X PBS) or goat anti-mouse IgG Alexa Fluor 594 (cat ...
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bioRxiv - Biophysics 2022Quote: ... incubated with secondary antibodies (anti-mouse IgG, Alexa Fluor 488, superclonal [Thermo Fisher Scientific, Cat#A28175], anti-rabbit IgG, Alexa Fluor 568 [Thermo Fisher Scientific, Cat#A11036]) at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation with polyclonal Alexa Fluor 488-or 594-conjugated goat anti-mouse or anti-rabbit secondary antibodies (Invitrogen Molecular Probes; Eugene, OR, USA). Alternatively ...
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bioRxiv - Physiology 2023Quote: ... samples were labeled with goat anti-mouse Alexa 647 (1:1000) and goat anti-rabbit Biotium CF 568 (1:2000) secondary antibodies (ThermoFisher Scientific, Grand Island, NY, USA). Simultaneous labeling with two primary antibodies from the same animal background was accomplished by completing the staining protocol with one set of primary and secondary antibodies followed by blocking with a donor secondary antibody (conjugated to fluorophore to assess degree of blocking ...
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bioRxiv - Systems Biology 2023Quote: ... samples were labeled with goat anti-mouse Alexa 647 (1:1000) and goat anti-rabbit Biotium CF 568 (1:2000) secondary antibodies (ThermoFisher Scientific, Grand Island, NY, USA). Where needed ...
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bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction obtained by centrifugation at 15,000 rpm for 15 min was reacted with sheep anti-mouse or anti-rabbit primary antibody-conjugated Dynabeads M280 (Thermo Fisher Scientific, Waltham, MA, USA) for 4 h or overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed three times with PBS and subjected to conjugated secondary antibody diluted in IFF buffer (anti-Mouse-AF488 A21202 Invitrogen, anti-Rabbit-568 A11036 Invitrogen, anti-Rat-AF647 A78947 Invitrogen) at 1:2000 for 2h at RT ...
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bioRxiv - Developmental Biology 2024Quote: ... The following secondary antibodies and dilutions were used: Donkey anti-Rabbit IgG (H+L) Alexa Fluor 488 (1:500, Thermo Fisher Scientific, catalog #A-21206), Donkey anti-Rat IgG (H+L ...
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bioRxiv - Physiology 2024Quote: ... Slices were then incubated with secondary antibodies (Cy3 Goat anti-Mouse, Jackson immunoresearch, 115-165-146, 1:1000; Donkey anti-Rabbit, Invitrogen, A-21206, 1:1000) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... add fluorescent secondary antibody (Goat anti-Mouse IgG, 1:500, Thermo Fisher Scientific # A-11001 or Goat anti-Rabbit IgG, 1:500, Thermo Fisher Scientific # A-11008) and incubate for 1 h at room temperature in a wet box ...
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bioRxiv - Cell Biology 2024Quote: ... The secondary antibodies used were goat anti-mouse and goat anti-rabbit conjugated to Alexa Fluor 568 or 488 (1:500, Thermo Fisher Scientific #A-11004, #11008). The cultures were washed with PBS ...
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... and subsequently incubated with secondary antibodies (goat anti-chicken-FITC, Jackson #103-095-155, 1:500; goat anti-rabbit Alexa-Fluor-594, Thermo Fisher Scientific, A11012; 1:500) and DAPI (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... We then washed and incubated slices for one hour with secondary antibodies diluted at a ratio of 1:200 (Alexa Fluor 488 goat anti-rabbit IgG (H+L) (Thermo Fisher Scientific Cat# A-11008); Alexa Fluor 594 goat anti-mouse IgG (H+L ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were washed with 0.01 M PBS three times for 10 mins each and then incubated in a 1:100 dilution of a rabbit-anti-goat antibody that was directly conjugated to AlexaFluor-546 (Invitrogen, Cat# A-21085, RRID: AB_2535742), 2% NDS and 0.5% Triton-X-100 in 0.1 M PBS for 2 hours ...
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bioRxiv - Microbiology 2024Quote: ... Secondary antibodies (goat anti-rabbit Alexa Fluor 488, Thermo Fisher Scientific, A11008, 1:2,000; goat anti-mouse Alexa Fluor 594, Thermo Fisher Scientific, A11032, 1:2,000) were diluted with TBS containing 0.2% Tween-20 and 3% BSA and incubated with coverslips for one hour at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... anti-rabbit Alexa Fluor 488 and rabbit Alexa Fluor 568 (1:1000; Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2020Quote: Secondary antibodies: Alexa Fluor secondary antibodies (Invitrogen) were used at a dilution of 1:1,000 ...
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bioRxiv - Biochemistry 2022Quote: ... and secondary antibody (Invitrogen anti-mouse antibody) binding ...
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bioRxiv - Neuroscience 2023Quote: Immunoprecipitation of Parkin was performed using rabbit polyclonal anti-Parkin (CST #2132) and the Dynabeads™ Protein G Immunoprecipitation Kit (Thermo Scientific #1007D) according to the manufacturers protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... For antibodies we used a His antibody (6x-His Tag Monoclonal Antibody, ThermoFisher), a MBP antibody (Anti-MBP Monoclonal Antibody ...
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bioRxiv - Cancer Biology 2019Quote: ... labeled cDNA targets were generated with the Encore® Biotin Module (Nugen) and hybridized to a GeneChip® Human Transcriptome Array 2.0 (Affymetrix, Thermo Fisher) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... and excess biotin was removed by dialysis in HEPES-based aCSF against 0.25 ml 2 mg/ml avidin Texas red (Thermo Fisher Scientific Cat# A820) through a Slide-A-Lyzer MINI (3500 molecular weight cut off ...
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bioRxiv - Microbiology 2021Quote: ... Fragments hybridizing to biotin-labelled HIV-1 DNA were pulled down by incubating the hybridization reaction with streptavidin-conjugated magnetic beads (ThermoFisher Scientific, Cat#88816) at room temperature for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... Biotin molecules were labeled at the N-terminal primary amino group (-NH2) of the bait proteins using N-hydroxysuccinimide (NHS)-biotin (ThermoFisher Scientific, Waltham, MA) in PBS at pH 6.5 ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins on the beads were eluted with 300 μl DLB containing 2 mM biotin and precipitated by adding 1/10 volume of 8.74 M Trichloroacetic acid solution (TCA, Fisher Scientific, Cat# BP555-500). Precipitated proteins were washed once with 500 μl of 0.874 M TCA and four times with ice-cold acetone ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained with human MR1-5-OP-RU tetramer for 40 minutes at RT in the dark prior to the addition of anti-bovine CD8-biotin (Clone ILA51) and Near-Infrared Fixable LIVE/DEAD stain (Invitrogen or Molecular Probes) for 20 minutes at 4 °C ...