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Citations for Thermo Fisher :
751 - 800 of 4628 citations for InP ZnS PEG NH2 Quantum Dots 640 nm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... falciparum parasites were incubated with 1 µM quarfloxin and either 50 nM DRAQ5 Abcam) or 100 nM Lysotracker DeepRed (ThermoFisher) or MitoTracker Deep Red FM (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... RNA was quantified fluorometrically at 260/290 nm and 260/230 nm using NanoDrop and the Qubit RNA HS Assay Kit (Invitrogen).
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bioRxiv - Molecular Biology 2022Quote: ... measurement (ratio of OD at 260 nm to OD at 280 nm is greater than 1.7) using a Nano-Drop spectrophotometer (Thermo Scientific). cDNA was synthesized using SuperScript IV Reverse Transcriptase (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... THP-1 were seeded in twenty-four-well plates for 24 h in the presence of 200 nM of PMA then transfected with 50 nM siRNA using Lipofectamine RNAIMAX (Invitrogen) according to the manufacturer’s instructions and incubated for a further 3 days ...
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bioRxiv - Biophysics 2024Quote: ... 40 nm dark-red beads (catalog no. F8789) and 40 nm red-orange beads (catalog no. F8794) were obtained from Invitrogen. Norland Optical Adhesive 74 (catalog no ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... was performed on Machery & Nagel Alugram® xtra SIL G/UV 254 visualization under UV light (254 nm) and/or (366 nm) and/or by dipping in anisaldehyde stain and subsequent heating.Commercial reagents and solvents (Acros Organics, Fluorochem ...
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bioRxiv - Microbiology 2023Quote: ... OD600 and fluorescence (λEx 485 nm/λEm 520 nm) was measured using using a Varioskan Flash microplate reader (ThermoFisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... OD600 and GFP fluorescence (excitation 485 nm, emission 520 nm) were measured using a Varioskan Lux plate reader (Thermo Scientific). GFP fluorescence relative to OD600 was determined ...
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bioRxiv - Biophysics 2023Quote: ... for different fluorescent color beads (40 nm diameter, red or dark red beads with emission maximum at 605 or 680 nm, F10720, Invitrogen) by illuminating with a 561 nm (or 640 nm ...
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bioRxiv - Biophysics 2023Quote: The fluorescence beads used in this study include: 40 nm red bead (580/605 nm, Thermo Fisher, cat. no. F8793), 100 nm red bead (580/605 nm ...
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bioRxiv - Cancer Biology 2023Quote: ... Lipophilic tracer 3,3′-dioctadecyloxacarbocyanine perchlorate (DiO, excitation, 488 nm; emission 525/50 nm) was purchased from Invitrogen (Carlsbad, CA, USA). Counting beads for flow cytometry experiments were purchased from Biolegend (424902 ...
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bioRxiv - Biochemistry 2022Quote: ... Day 1 – 0.5 x 105 cells were seeded on fibronectin-coated coverslips in a 24-well plate for immunofluorescence or 3 x 105 cells in a 6-well plate for western blotting and transfected with 20 nM siCAPN7 and 10 nM siNup153 complexed with Lipofectamine RNAiMAX (Invitrogen) (See Supplementary Table 3 for sequences ...
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bioRxiv - Biochemistry 2022Quote: ... generated from Point Spread Functions (PSFs) acquired from 100 nm green and red FluoSpheres and alignment parameters refined using 100 nm TetraSpeck beads (Invitrogen).
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bioRxiv - Cell Biology 2022Quote: ... MDM were seeded at 1×105 cells/well and transfected with 60 nM total Silencer Select NHLRC2 (20 nM each of s51529, s51530 and s51531) siRNAs (Ambion) according to the method developed by Fisch et al ...
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bioRxiv - Genomics 2022Quote: DNA quality was measured using the spectrometry absorbance ratios of 260/280 nm and 260/230 nm (NanoDrop, Thermofisher Scientific) respectively ...
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bioRxiv - Neuroscience 2022Quote: ... cells were incubated for 1 min with F(ab’)2-Goat anti-Rabbit IgG (H+L) Secondary Antibody QDot emitting at 655 nm (1 nM; Invitrogen) in PBS (1 M ...
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bioRxiv - Neuroscience 2023Quote: ... Optical resolution was assessed by imaging fluorescent beads (yellow-green fluorescent beads, 40 nm or 170 nm in diameter, Invitrogen) immobilized on glass slides.
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with either 0.1% DMSO or 7 nM bortezomib and 30 min prior to fixation cells were treated with MitoTracker Deep Red (500 nM, Invitrogen). 16 % paraformaldehyde (EMS ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the purity of the extracted DNA (OD 260/280 nm and 260/230 nm ratios) was measured using a Nanodrop 1000 spectrophotometer (Thermofisher). To quantify Wolbachia load ...
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bioRxiv - Cell Biology 2024Quote: ... calcein blue (0.1 mg/mL, 405 nm (M1255)) and cell mask deep red (5 μg/mL, 630 nm (ThermoFisher, C10046)) were added to the staining medium for 2h ...
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bioRxiv - Molecular Biology 2024Quote: ... Cohesin was imaged in CMG buffer supplemented with 150 nM SYTOX Orange or 5 nM YOYO-1 (both Thermo Fisher) and an oxygen scavenging system (see below) ...
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bioRxiv - Molecular Biology 2024Quote: Knockdown of MALAT1 was done in two rounds of ASO treatment at a final concentration of 200 nM and 100 nM using Lipofectamine RNAiMax reagent (Invitrogen). After the second round of knockdown ...
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bioRxiv - Neuroscience 2024Quote: ... Probes were excited using a Polychrome V monochromator (ex: 488 nm, em: >505 nm; Thermo Fisher Scientific/FEI, Planegg, Germany) at a framerate of 1 Hz [9].
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bioRxiv - Biophysics 2024Quote: ... 1/1000 by volume of carboxylate red fluorosphere fluorescent beads (580 nm/605 nm excitation/emission wavelength, Thermo Fisher, F8801) were added to 495 microliters of the mix and placed in a vacuum dessicator for 15 minutes to reduce dissolved oxygen before addition of 5 uL of 10% by weight of APS (freshly prepared in diH2O ...
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bioRxiv - Biochemistry 2024Quote: ... followed by an enzyme solution consisting of 8 nM wild-type PETase or its mutant labeled with 0.8 nM Qdot655 (Thermo Scientific), 2 mM 2-Mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... and THP-1 cells were incubated with GZMK (50 nM) or C1s (50 nM) for 1 h on ice at 4 °C in HBSS with Ca2+ and Mg2+ (Gibco) containing 10 mM HEPES and 0.5% BSA (Millipore Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... Fluorescence levels (excitation 565 nm/emission 590 nm) were measured using a Varioskan LUX Multimode Microplate Reader (ThermoFisher Scientific, #VLBL00GD2).
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bioRxiv - Biochemistry 2024Quote: ... The measurements were done by a fluorescent reader at 360 nm excitation/460 nm emission (Varioscan, Thermo Fisher Scientific, US). DMSO was used as an experimental control ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration was measured via UV absorbance at 280 nm and 565 nm using a Nanodrop One Spectrophotometer (Thermo Scientific).
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bioRxiv - Cancer Biology 2024Quote: ... 100 nM p53-Y220C-BODIPY-FL-SpyCatcher S50C (or 100 nM p53-WT-BODIPY-FL-SpyCatcher S50C) and 2 nM terbium-coupled streptavidin (Invitrogen) in a buffer containing 50 mM Tris pH 7.5 ...
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bioRxiv - Developmental Biology 2024Quote: ... The signal intensity of fluorescence excited at 535 nm for 1 second was measured at 587 nm using Varioskan flash (ThermoFisher). The readouts from standard were plotted to construct a standard curve for estimating methionine concentration.
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bioRxiv - Cancer Biology 2024Quote: ... Purity was assessed by measuring absorbance at 260 nm and 280 nm using a NanoDrop 2000 Spectrophotometer (ThermoFisher Scientific; RRID:SCR_018042). For genotype profiling ...
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bioRxiv - Systems Biology 2021Quote: ... the crosslinking of 5’ thiol-modified 18-nt DNA oligonucleotides (Integrated DNA Technologies) to lysine residues on antibodies was performed via PEGylated SMCC cross-linker (SM(PEG)2) (Thermo Scientific Thermo Scientific 22102). As an alternative conjugation strategy ...
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bioRxiv - Microbiology 2021Quote: Two-day biofilm formation of Pseudomonas strains was determined by crystal violet staining using a 96-well plate lid with pegs extending into each well (Nunc-TSP lid, Invitrogen™ Thermo Fisher Scientific). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... Products from the folding reactions were mixed with an equal volume of PEG precipitation buffer containing 15% PEG8000 (Fisher Scientific, Cat No. BP233), 10 mM Tris pH 8.0 ...
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bioRxiv - Genomics 2023Quote: ... we used the crosslinking of 5’ thiol-modified 18-nt DNA oligonucleotides (Integrated DNA Technologies) to lysine residues on antibodies via PEGylated SMCC cross-linker (SM(PEG)2) (Thermo Scientific Thermo Scientific 22102) 7 ...
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bioRxiv - Plant Biology 2023Quote: ... For each transformation 63 µL of protoplast suspension was mixed with 25 µL of DNA in 0.1 M Ca(NO3)2 and 88 µL of 40% (w/w) PEG solution in a round-bottom 1 mL 96 deep-well plate (Nunc®, Roskilde, Denmark). The PEG solution was prepared by mixing 8 g of PEG (polyethylene glycol ...
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bioRxiv - Microbiology 2023Quote: Two-day biofilm formation of Pseudomonas strains was determined by crystal violet staining using a 96-well plate lid with pegs extending into each well (Nunc-TSP lid, Invitrogen™ Thermo Fisher Scientific). Briefly ...
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bioRxiv - Immunology 2024Quote: ... Activated T cells were then fused with the TCRα-β- BW5147 fusion partner in a 1:1 ratio using polyethylene glycol (PEG)1500 (Thermo Scientific Chemicals). Fused cells were cultured in selection medium containing HAT supplement (1/50 dilution – purchased from Gibco ...
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bioRxiv - Biophysics 2020Quote: ... Multi-color 100 -nm fluorescent beads (Tetraspeck, Invitrogen) were used to register long-term acquisitions and correct for lateral drift ...
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bioRxiv - Cell Biology 2020Quote: ... 10 nM MitoTracker Orange CMTMROS (Molecular Probes/ThermoFisher) were added to cultures of control and TbRrp44 knockdown cells 72 hours after RNAi induction ...
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bioRxiv - Cell Biology 2020Quote: ... 10 nM MitoTracker Orange CMTMROS (Molecular Probes/ThermoFisher) were added to cultures of control and TbRrp44 knockdown cells 72 hours after RNAi induction ...
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bioRxiv - Cell Biology 2020Quote: ... with a 594-nm anti-rabbit IgG (Invitrogen). The tertiary spray used Hoechst instead of an antibody.
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bioRxiv - Immunology 2021Quote: ... and 25 nM SYTOX Green (Thermo Fisher Scientific), were incubated for 4 hours at 37 °C with 5% CO2 with stimulants in low attachment 1.5 mL tubes (PROKEEP ...
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bioRxiv - Immunology 2021Quote: ... and 500 nM Sytox Green (Thermo Fisher Scientific) as a membrane-impermeable DNA stain ...
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bioRxiv - Developmental Biology 2021Quote: ... 100 nM LysoTracker (Red DND-99, Molecular Probes), and 10 ng/µl Hoechst 33342 for one hour at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... and 50 nM 2-mercaptoethanol (Gibco 31350-010) at density of 3-10×105 cells per cm2 in culture flasks coated with 0.1% gelatine (Smith ...
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bioRxiv - Immunology 2022Quote: ... 2 nM of GFP Silencer® siRNA (Ambion) or 2 nM of the negative control Silencer® siRNA (Ambion ...
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bioRxiv - Immunology 2021Quote: ... lysoTracker Red DND-99 (Thermo Fisher, 75 nM) was added to the cells for 1 h at 37 °C ...
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bioRxiv - Biophysics 2021Quote: ... 100-nm Tetraspeck beads (Thermo Fisher Scientific T7279) were rastered across a glass coverslip ...