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Citations for Thermo Fisher :
701 - 750 of 4628 citations for InP ZnS PEG NH2 Quantum Dots 640 nm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... was prepared using a polyethlene glycol (PEG) precipitation protocol.51 DNA purity and concentrations for relevant experiments were measured with a NanoDrop 2000 (Thermo Fisher Scientific).
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bioRxiv - Genomics 2020Quote: ... The plate was heated at 65° C for 5 minutes and transferred to ice prior to the addition of 1 ul of 2X Reverse transcription mix (15% PEG 8000 [Sigma Aldrich], 2X Maxima RT buffer [Thermo Fischer] and 4 units of Maxima H Minus RT [Thermo Fisher]). cDNA synthesis was performed for 15 min at 50°C followed by 5 min at 85° C for inactivation ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were either directly injected from the 384 well plate or transferred to a PEG coated PCR tubes (PCR-02-L-C, Axygen®) or to the autosampler glass vials (601801655, Thermo Scientific).
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bioRxiv - Immunology 2022Quote: The peak fractions from gel filtration chromatography were concentrated to a final concentration of 0.9 mg/ml and crosslinked by 0.4 mM BS(PEG)5 (Thermo Scientific, Catalog number A35396) on ice for 40 min before cryo-EM sample preparation ...
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bioRxiv - Bioengineering 2023Quote: ... TRACE support bath was created by thoroughly mixing 5 parts of the resulting agarose slurry with 1 part of 800mg/mL PEG 8000 solution in PBS (Gibco, NY, USA) to obtain a final PEG concentration of 200mg/mL ...
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bioRxiv - Biochemistry 2024Quote: ... The amine group of the purified DNA constructs was further modified to maleimide by incubating the sample with 1 mM SM(PEG)2 (Thermo Scientific Pierce) for 20 min at ∼23°C ...
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bioRxiv - Plant Biology 2023Quote: ... were then transferred to ½MS as a normal condition or to ½MS infiltrated with 25% Polyethylene-glycol (PEG) 8000 (Fisher Scientific, Belgium) to induce drought stress ...
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bioRxiv - Cancer Biology 2023Quote: ... fluorophore-conjugated secondary antibodies (Goat anti-Rabbit IgG (H+L) Dylight™ 800 4X PEG and Goat anti Mouse IgG (H+L) Dylight™ 680 secondary antibodies from Invitrogen, Thermofisher ...
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bioRxiv - Bioengineering 2023Quote: ... The liquid holders were surface-treated with oxygen plasma for 2 minutes at 0.2 Torr using a plasma generator (Plasma Etch, Inc., PE-25) and polyethylene glycol (PEG) 400 (Fisher Scientific, P167-1) for 24 hours ...
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bioRxiv - Immunology 2024Quote: Secondary antibodies for Western blotting were DyLight 800 4× PEG-conjugated goat anti-rabbit IgG(H+L) (catalog number SA5-35571; Thermo Fisher Scientific) (1:10,000) ...
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bioRxiv - Neuroscience 2020Quote: ... Absorbance was measured at 450 nm and 570 nm using the Multiskan Ascent 354 microplate reader (Thermofisher, Waltham, USA). Concentrations were determined using the standard curve generated using known concentrations of TNFα and IL-6.
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bioRxiv - Biophysics 2020Quote: ... The grids were subsequently coated with 100 nm and 500 nm fluorescent microspheres (TetraSpeck Microspheres, Invitrogen T7279, T7281, USA) at a 500x or 1000x dilution for 5 min ...
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bioRxiv - Bioengineering 2022Quote: ... The absorbance at 450 nm (signal) and 570 nm (background) was then measured with a microplate photodetector (Fisher Scientific).
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bioRxiv - Plant Biology 2021Quote: ... The OD at 645 nm and 663 nm for chlorophyll a and chlorophyll b were measured with NanoDrop (Thermofisher). The Chlorophyll concentration was calculated with the Arnon’s equations ...
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bioRxiv - Molecular Biology 2024Quote: ... miRNA mimic (20 nM) and miRNA negative control (20 nM) were transfected into cells using Lipofectamine 2000 (Invitrogen, USA) according to the instructions of the reagent ...
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bioRxiv - Molecular Biology 2024Quote: ... and mitochondria with either 100 nM MTY for 15 min or 100 nM MitoTracker Green FM (MTG, # M7514, Invitrogen) for 30 min at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... and fluorescence intensity was measured at (λex) of 485 nm and (λem) at 535 nm using Fluoroskan (Thermo Scientific).
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bioRxiv - Immunology 2023Quote: ... the cells were harvested and transfected with either 20 nM non-target control siRNA or 20 nM AMPKα1 (both Dharmacon) or FoxO3 (Invitrogen) siRNA using Neon Transfection System (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... expressing either GPI-GFP or YFP-CB1 were then incubated with increasing amounts of QD-NB-conjugates (between 0.1 nM and 1 nM) in live cell imaging solution (#A14291DJ, Thermofisher) until desired QD density was reached depending on cell density and transfection efficiency ...
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bioRxiv - Bioengineering 2023Quote: ... The z-stack images of orange (540/560 nm) and deep-red (633/660 nm) fluorescent beads (P7220, Invitrogen) mounted in OI or WI media were acquired and processed in SMAP [26] for 3D point spread function (PSF ...
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bioRxiv - Cancer Biology 2023Quote: ... or increasing concentrations of gemcitabine (dose range = 10 nM to 1μM) (Catalog: G6423-50MG, MilliporeSigma) or paclitaxel (dose range = 10 nM to 1μM) (Catalog: AAJ62734MC, Thermo Fisher) was added to each well ...
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bioRxiv - Plant Biology 2023Quote: ... followed by an enzyme solution consisting of 2 nM Cel7A labeled with 0.5 nM Qdot (Thermo Scientific; Cas: Q10143MP), 5 mM dithiothreitol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the absorbances of the extracted RNAs at 260 nm and 280 nm were determined spectrophotometrically (Nanodrop, Thermofisher Scientific). RNA samples with purity ratios (A260/A280 ...
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bioRxiv - Microbiology 2023Quote: ... Fluorescence (excitation of 450 nm, emission of 670 nm) was measured using a Varioskan Lux plate reader (Thermo Scientific).
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bioRxiv - Neuroscience 2024Quote: ... was added at a final concentration of 20 nM along with 0.5 μL 100 nm-sized Tetraspeck beads (Invitrogen) for 15 min at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... dasatinib (150 nM) and dexamethasone (500 nM) alone or in combination for 24 hours and total RNA was isolated using Trizol (Invitrogen).
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bioRxiv - Cell Biology 2020Quote: ... reverse transfection using 80 nM of silencer RNA (siRNA) for GTSE1 and 100 nM of siRNA for EB1 was performed using Oligofectamine (Invitrogen). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... Z-calibration and sample drift corrections were done using a few 40 nm TransFluoSphere beads (488/685 nm, Life Technologies) as fiducial markers spread on the cell samples.
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bioRxiv - Molecular Biology 2020Quote: 6xHis-tagged REV-ERBβ LBD (4 nM) was incubated with anti-His antibody conjugated to terbium (1 nM) (ThermoFisher #PV5863) and FITC-labeled NCoR ID1 or NCoR ID2 peptide (400 nM ...
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bioRxiv - Neuroscience 2020Quote: ... slides were incubated with fluorophore-conjugated secondary antibodies (Alexa Fluor goat-anti rabbit 488 nm, goat anti-rat 594 nm, Invitrogen) for 1 h at RT ...
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bioRxiv - Systems Biology 2021Quote: ... miR-291a-3p (20 nM final concentration), or a combination of both (10 nM final concentration, each) using the Lipofectamine RNAiMax transfection reagent (Invitrogen), according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... and RNA purity was analysed by comparing 260/230 nm and 260/280 nm absorbance ratios (Nanodrop 2000, Thermo Fisher). RNA was quantified using a Qubit 2.0 fluorometer (Thermo Fisher) ...
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bioRxiv - Biophysics 2020Quote: ... We choose the excitation and emission wavelengths to be 495 nm and 537 nm (excitation and emission maxima for SYBR Gold, as provided by Invitrogen). For control measurements ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected twice with 10 nM Silencer Select BRCA2 s2084 siRNA and 10 nM Silencer Select BRCA2 s2085 siRNA (Ambion) using Lipofectamine RNAiMAX (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... The measurements were performed by a fluorescent reader at 360 nm excitation/460 nm emission (Varioscan, Thermo Fisher Scientific, US). While DMSO was used as solvent control ...
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bioRxiv - Immunology 2022Quote: ... the plates were read at multiple wavelengths (450 nm and 550 nm) on a plate reader (Multiskan GO, Thermo Fisher). The raw data were processed by Prism 9 (GraphPad ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were transfected with 20 nM siRNA oligonucleotides and 20 nM negative control siRNA using Lipofectamine RNAiMax (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed 3 times with PBS and incubated for 1 hour with Alexa 488 nm or Alexa 594 nm conjugated secondary antibodies specific for human and rat IgG (Invitrogen) diluted 1:2,000 in 3% BSA in PBS and containing 1 μg/ml DAPI ...
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bioRxiv - Biophysics 2022Quote: Molten M9DCAA 0.6% agar was mixed with 0.5 µm-diameter fluorescent microsphere (FluoSpheres carboxylate-modified, ex/em: 580 nm /605 nm; Thermofisher F8812) at a final microsphere number density of 3.1 x1011 particles/mL (∼1000-fold dilution of the microsphere solution) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were grown in antibiotic free media and transfected with 20 nM mimic when transfected singly or 10 nM respective mimic for dual transfections using RNAimax reagent (Invitrogen). Cells were harvested 39 hours post transfection and duplicate samples were pooled for protein extraction and subsequent western blot analysis ...
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bioRxiv - Biophysics 2021Quote: Yellow-green carboxylate fluoSpheres (REF F8803, 2% solids, 100 nm diameter, exc./em. 505/515 nm, Invitrogen, Thermo Fisher Scientific) were diluted 1000× in ultrapure water and sonicated for 10 minutes in a water bath sonicator (Labsonic LBS1-0.6 ...
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bioRxiv - Biophysics 2021Quote: Yellow-green carboxylate fluoSpheres (REF F8803, 2% solids, 100 nm diameter, exc./em. 505/515 nm, Invitrogen, Thermo Fisher Scientific) were diluted 1000× in ultrapure water and sonicated for 10 minutes in a water bath sonicator (Labsonic LBS1-0.6 ...
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bioRxiv - Bioengineering 2020Quote: ... The Cas12a-crRNA ribonucleoprotein complex was assembled by mixing 100 nM Cas12a and 100 nM crRNA in 1X NEBuffer 2.1 in the Proflex PCR system (ThermoFisher Scientific) at 25°C for 15 minutes (volume 28.5 µL) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with 20 nM siRNA oligonucleotides and 20 nM Negative Control siRNA using Lipofectamine(tm) RNAiMax (ThermoFisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... 530 and 657 nm (anthocyanins) or 320 nm (phenolic compounds) in a Multiskan Spectrum (Thermo Fisher Corp., Waltham, MA, U.S.A.). Chlorophyll concentrations were calculated using the following equations ...
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bioRxiv - Biochemistry 2020Quote: ... Beta-lactamase activity was determined at 390 nm (yellow) and 486 nm (red) using a VarioSkan plate reader (Thermo Scientific).
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bioRxiv - Microbiology 2021Quote: ... of the system was measured under the same imaging conditions (with a 50 nm z-step size) using 100 nm fluorescent TetraSpeck microspheres (Invitrogen) embedded in 1% agarose gels ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples at the Cambridge laboratory were stained with a 1:2500 dilution (8 µM) of Hoechst 33342 (peak excitation: 351 nm, peak emission: 461 nm) (#62249, ThermoFisher) for 30 mins ...
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bioRxiv - Neuroscience 2021Quote: ... Optical resolution was assessed by imaging fluorescent beads (yellow-green fluorescent beads, 40 nm or 170 nm in diameter, Invitrogen) immobilized on glass slides (Fig ...
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bioRxiv - Biochemistry 2022Quote: ... Day 1 – 0.5 x 105 cells were seeded on fibronectin-coated coverslips in a 24-well plate for immunofluorescence or 3 x 105 cells in a 6-well plate for western blotting and transfected with 20 nM siCAPN7 and 10 nM siNup153 complexed with Lipofectamine RNAiMAX (Invitrogen) (See Supplementary Table 3 for sequences ...