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Citations for Thermo Fisher :
551 - 600 of 4628 citations for InP ZnS PEG NH2 Quantum Dots 640 nm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... samples were indexed by ligating TruSeq Illumina adaptors by incubating DNA-bound beads in 50 μl adapter ligation mix (1x T4 Ligation buffer, 5% PEG-4000, 0.3 U/ μl T4 DNA Ligase (ThermoFisher, EL0011), 1.5 μl TruSeq index adapter) ...
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bioRxiv - Systems Biology 2020Quote: ... The samples were then further post-fixed with 1.5 mM BS(PEG)5 (PEGylated bis(sulfosuccinimidyl)suberate) (Thermo Scientific A35396) in 1× PBS at room temperature for 20 minutes ...
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bioRxiv - Microbiology 2021Quote: ... (500 mM Tris-HCl [pH 7.5], 50 mM MgCl2, 50 mM dithiothreitol [DTT] [Bio-Rad], 31.25 mM polyethylene glycol 8000 [PEG 8000] [Fisher Scientific] ...
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bioRxiv - Microbiology 2021Quote: Two-day biofilm formation of Pseudomonas strains was determined by crystal violet staining using a 96-well plate lid with pegs extending into each well (Nunc-TSP lid ...
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bioRxiv - Developmental Biology 2022Quote: Organoids were treated immediately after PEG embedding for 3 days with neural differentiation medium comprised of a 1:1 mixture of neurobasal medium (GIBCO) and DMEM/F12 (GIBCO) ...
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bioRxiv - Developmental Biology 2022Quote: Organoids were treated immediately after PEG embedding for 3 days with paraxial mesoderm differentiation medium comprised of DMEM/F12 (GIBCO), 1% N2 (GIBCO) ...
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bioRxiv - Biophysics 2022Quote: ... The biotin-PEG layer was functionalized by incubating for 15 min with a 0.2 mg/mL solution of neutravidin (Thermo Scientific). Channels were washed with 1 mL of HB to remove excess neutravidin ...
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bioRxiv - Microbiology 2022Quote: ... the robot adds 2 volumes of 30% PEG 6000 (in 1.6 M NaCl) and 35ul of 1:5 diluted Glycoblue (Invitrogen, Thermo Fisher) to each well ...
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bioRxiv - Plant Biology 2022Quote: ... H3.3-3xFlag (AT4G40040) in-frame fragments were respectively delivered into the binary vector pEG using the LR reaction kit (Invitrogen). Genome-wide distribution of H3.1-3xFlag and H3.3-3xFlag were consistent with previously published ChIP-seq of H3.1/H3.3 fused with 4xMyc-tag5 ...
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bioRxiv - Microbiology 2023Quote: ... The biotin-PEG layer was functionalized by incubating 15 min with a 0.2 mg/mL solution of neutravidin (Thermo Scientific). Channels were washed with 1mL of HB to remove excess neutravidin ...
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bioRxiv - Biochemistry 2022Quote: ... was created by sticking the PLL-PEG passivated side of the coverslip to a glass slide (Super Frost, Thermo Scientific) using a double-sided adhesive tape ...
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bioRxiv - Biophysics 2023Quote: ... the purified DNA in 1 ml NaHCO3 (pH 8.3) was incubated with 1 mM SM(PEG)2 (Thermo Scientific Pierce) for 20 mins at 20-22°C ...
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bioRxiv - Microbiology 2023Quote: Two-day biofilm formation of Pseudomonas strains was determined by crystal violet staining using a 96-well plate lid with pegs extending into each well (Nunc-TSP lid ...
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bioRxiv - Microbiology 2024Quote: ... The plates were covered by the lid with pegs (Nunc – Transferable Solid Phase screening system (Nunc-TSP, Thermo Fisher Scientific), which submerged into the bacterial cultures in the wells and were incubated overnight at 37°C to form biofilms on the Nunc-TSPs of the lid.
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bioRxiv - Microbiology 2024Quote: ... The plates were covered by the lid with pegs (Nunc – Transferable Solid Phase screening system (Nunc-TSP, Thermo Fisher Scientific), which submerged into the bacterial cultures in the wells and were incubated overnight at 37°C to form biofilms on the Nunc-TSPs of the lid.
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bioRxiv - Microbiology 2024Quote: Bowel prep solution was prepared by dissolving 0.05 M polyethylene glycol (PEG) (commercially branded Restoralax) in DMEM high glucose or Hanks’ Balanced Salt Solution (HBSS, Thermo Fisher). After 2 days of culture in antibiotic-free media ...
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bioRxiv - Genomics 2021Quote: ... The 260/280 nm and 260/230 nm absorption ratios were further tested on Nanodrop (Applied Biosystems) to investigate for their concentration and purity ...
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bioRxiv - Molecular Biology 2024Quote: ... The absorbance was measured at 490 nm and 680 nm with a spectrophotometer (Multiskan Ascent, Thermo Scientific). The experiment was performed in three independent biological replicates by triplicates ...
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bioRxiv - Immunology 2023Quote: ... T cells were loaded with 500 nM MitoTracker DeepRed and 2 nM Tetramethylrhodamin-Ethylester (TMRE) (both Invitrogen). As control for TMRE ...
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bioRxiv - Biophysics 2023Quote: ... 100 nm diameter blue beads with an excitation wavelength (λex) of 450 nm (Fluoro-Max; Fisher Scientific), 100 nm diameter yellow bead ...
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bioRxiv - Microbiology 2023Quote: ... The fluorescence (excitation 512 nm, emission 527 nm) of each strain (200 µl – Nunc black well plates) was read in triplicate using an Ensight multimode plate reader (PerkinElmer ...
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bioRxiv - Biochemistry 2024Quote: ... Optical density was measured at 595 nm and 650 nm with a microplate spectrophotometer (Multiskan, Thermo Scientific). Experiments were repeated twice in quintuplicate ...
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bioRxiv - Neuroscience 2022Quote: ... Accela UPLC pump coupled to a Quantum Access triple quadrupole mass spectrometer equipped with a heated electrospray (HESI) probe (Thermo Scientific, San Jose, CA, USA). The capillary and spray temperatures were both set to 300°C and the electrospray capillary voltage to 4 kV ...
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bioRxiv - Biochemistry 2023Quote: ... Data acquisition was performed using an Ultimate 3000 HPLC system interfaced with a TSQ Quantum Access MAX triple quadrupole Mass Spectrometry (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Biophysics 2021Quote: ... and biotin-PEG-SVA at a ratio of 99:1 (w/w) (Laysan Bio) in 0.1 M sodium bicarbonate (Thermo Fisher Scientific) for 3 h.10 Excess PEG was rinsed with water ...
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bioRxiv - Cell Biology 2021Quote: ... Collected medium with virus particles were centrifuged at 1,000 x g for 15 minutes at 4 °C and the supernatant were mixed with 4X lentivirus concentration solution −40% (w/v) polyethylene glycol 8000 (PEG 8000, Fisher Scientific), 1.2 M NaCl ...
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bioRxiv - Neuroscience 2022Quote: Peptides were constructed on a CEM Liberty 12-Channel Automated Microwave Peptide Synthesizer using Fmoc-PAL-PEG-PSTM (Rink resin; loading capacity 0.21 mmol/g) (ThermoFisher, Cat.#: GEN913383) and Fmoc-protected amino acids (GL Biochem (Shanghai)) ...
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bioRxiv - Microbiology 2022Quote: ... the robot adds 2 volumes of 30% PEG 6000 (in 1.6 M NaCl) and 35ul of 1:5 diluted Glycoblue (Invitrogen, Thermo Fisher) to each well ...
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bioRxiv - Immunology 2024Quote: ... 1 × TE buffer (5 mM Tris base, pH 8.0 and 1 mM EDTA acid) containing 15% w/v PEG-8000 (Fisher Scientific, BP2331) and 510 mM NaCl was added to the SQB sample at 1:1 volume and mixed gently via pipetting ...
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bioRxiv - Cell Biology 2024Quote: ... to form condensates (with 20% v/v PEG 8 kDa as needed) and overlaid with fluorescent dextrans of various sizes at 10 kDa (Invitrogen D1816), 40 kDa (Invitrogen D1842) ...
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bioRxiv - Plant Biology 2024Quote: ... the protoplast-containing Trizol solution from PEG transformation was transferred to 2 mL Phasemaker tubes (1 mL per tube; ThermoFisher Scientific), mixed thoroughly with 300 µL chloroform ...
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bioRxiv - Cancer Biology 2024Quote: ... AAV-SF3B1-K700E virions were released from HEK293T using the freeze-thaw method and precipitated overnight at 4°C using PEG 8000 (Fisher Scientific). Precipitates were pelleted via centrifugation at 2800 g for 15min at 4°C ...
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bioRxiv - Biophysics 2023Quote: 7 μM Aβ40 was incubated with varying stoichiometries of claramine and 5% PEG (50% w/w 10 kDa, Thermo Fisher Scientific) in the presence of 20 mM ThT (Sigma-Aldrich ...
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Fine tuning of CpG spatial distribution with DNA origami for improved therapeutic cancer vaccinationbioRxiv - Synthetic Biology 2023Quote: ... 1 × TE buffer (5 mM Tris, pH 8.0 and 1 mM EDTA) containing 15% (w/v) PEG-8000 (Fisher Scientific, BP2331), and 510 mM NaCl was added to the origami sample at 1:1 volume in an Eppendorf tube and mixed gently ...
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bioRxiv - Molecular Biology 2023Quote: ... This was followed by six washes with PBS and an additional post-fixation step using 1.5 mM BS(PEG)5 (Thermo Scientific, A35396) in PBS for 20 minutes at room temperature ...
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bioRxiv - Genomics 2024Quote: ... was prepared fresh with the 3’aminated 5’ caged oligo root carrying a terminal Cy3 dye (2 μM BL003 for the single-cycle experiment and 2 μM BL728 for the 4-cycle experiment) and a bifunctional crosslinker BS(5)PEG (Thermo Scientific) (5 μM in dimethylformamide) ...
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bioRxiv - Microbiology 2024Quote: ... purified in PEG (polyethylene glycol) 8000 (Gias et al., 2008) and frozen in 3% sucrose in Dulbecco’s Modified Eagle Medium (Invitrogen, Carlsbad, CA). SARS-CoV-2 Wuhan strain was obtained from Beiresources and expanded in Vero E6 cells (Beiresources) ...
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bioRxiv - Microbiology 2024Quote: Viruses were precipitated from agar stocks by mixing with polyethylene glycol 8000 (PEG 8000, Thermo Scientific, final concentration 10% [w/v]) and NaCl (final concentration 0.5 M ...
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bioRxiv - Biophysics 2024Quote: ... wild-type α-synuclein was mixed with an A90C variant labelled with Alexa 647 at a 100:1 molar ratio in 50 mM Tris-HCL and 5% polyethylene glycol 10,000 (PEG) (Thermo Fisher Scientific). The final mixture was pipetted onto a 35-mm glass-bottom dish (P35G-1.5-20-C ...
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bioRxiv - Molecular Biology 2024Quote: ... The secondary antibodies used were goat anti-rabbit IgG (H+L) Secondary Antibody DyLight™ 800 4X PEG (Invitrogen, SA5-35571) (1:10,000 ...
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bioRxiv - Plant Biology 2024Quote: ... the protoplast-containing Trizol solution from PEG transformation was transferred to 2 mL Phasemaker tubes (1 mL per tube; ThermoFisher Scientific), mixed thoroughly with 300 µL chloroform ...
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bioRxiv - Biochemistry 2024Quote: ... Protein fractions and final Pol I preparations were quality controlled by SDS-PAGE and Western analysis using Coomassie staining and anti-TAP-tag antibody (TAP Tag Monoclonal Antibody DyLight™ 800 4X PEG, clone 22F12-1E3, cat.no. 15342907, Fisher Scientific), respectively.
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bioRxiv - Bioengineering 2020Quote: ... media was removed and replaced with 30 µL 5 nM PLA probes (2.5 nM probe A and 2.5 nM probe B) in Probe Binding Buffer (PBS, 0.1% BSA (Thermo Scientific), 0.1 mg/ml sonicated salmon sperm DNA (Invitrogen) ...
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bioRxiv - Genetics 2022Quote: ... equipped with the GFP Light Cube (470/22 nm Excitation; 510/42 nm Emission) (ThermoFisher Scientific; Catalogue #AMEP4651).
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bioRxiv - Plant Biology 2021Quote: Qdot-labeled Cel7A was prepared by mixing 2-10 nM Cel7A with 0.5 nM Qdot 525 (Thermo Scientific) in 50 mM sodium acetate ...
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bioRxiv - Microbiology 2021Quote: ... Channels were aligned prior to imaging using 40 nM TransFluoSphere streptavidin-labeled beads (488/645 nm, Life Technologies) as fiducial markers ...
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bioRxiv - Plant Biology 2020Quote: ... Sample loading was visualised via SyproRuby stain (Ex. 450 nm, Em. 610 nm; SyproRuby Invitrogen, Carlsbad, California, USA), performed according to manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... and Alexa Fluor® 488 (0.5 mg/ml, λex: 495 nm, λem: 519 nm; ThermoFisher Scientific #D-22910) diluted in glucose-free modified Krebs buffer ...
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bioRxiv - Neuroscience 2022Quote: Human BDNF-biotin (50 nM) was incubated with 50 nM Qdot-605 Streptavidin conjugate (Thermo Fisher Scientific; Q10101MP) on ice for 1 h in Neurobasal medium to facilitate the biotin-streptavidin interaction ...
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bioRxiv - Microbiology 2022Quote: ... consisting of 0.01 nM PstI-linearized pGEX-6p DNA (Cytiva) (0.02 nM ends) and PicoGreen™ (1:20,000 dilution, Thermofisher) in 20 mM HEPES (pH 7.2) ...