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Citations for Thermo Fisher :
7801 - 7850 of 10000+ citations for West Nile Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... The pellet was re-suspended in 8M urea/50mM Tris pH8 and protein concentration was determinate with Qubit Protein Assay (Invitrogen).
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bioRxiv - Cancer Biology 2021Quote: ... phosphatase inhibitors) and proteins concentrations were measured using a Pierce BCA protein Assay Kit (Thermo Fisher Scientific, Ref: 23225/23227). 10 μg of proteins were then separated on a 4-15% Mini-PROTEAN TGX Stain-Free Gel (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein samples (50µg total protein) were resolved by SDS-PAGE using 4-12% Bis-Tris gel (Nupage, Invitrogen, Carlsbad, CA) and transferred to a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... the protein was centrifuged at 10,000×g for 10 min and quantified by BCA Protein Assay Kit (Thermo Fisher Scientific). The same quantity (about 15 μg ...
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bioRxiv - Cell Biology 2020Quote: ... per 1 mg of total protein were added along with 30 μL washed protein A/G UltraLink Resin (53133, ThermoFisher) and rotated overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... Total protein amount was measured from 10 µL of the sample using the Pierce BCA protein assay kit (Thermo Fisher). The levels of radioactive methionine were measured by scintillation counting with 10-80 µL of the resuspended protein ...
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bioRxiv - Cell Biology 2022Quote: ... DNA- protein complexes were pulled down with addition of 10 μl of pre-washed Protein G Magnetic Beads (Invitrogen, 10003D) and incubation for 1-2 hours at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was clarified by centrifugation and the total protein concentration was measured with the BCA Protein Assay (Thermo Fisher Scientific) to enable input standardization ...
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bioRxiv - Cell Biology 2022Quote: ... was used to lyse cell pellets and protein concentration determined using the Micro BCA Protein Assay kit (ThermoFisher Scientific, 232350). 10 μg protein ...
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bioRxiv - Neuroscience 2022Quote: ... Both the protein extracts from cell pellets and the supernatants from cultured cells were subjected to protein quantification using BCA Protein Assay Kit (Thermofisher). Samples were boiled at 99 °C for 2 min ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Total protein concentrations of the samples were determined by the Pierce™ bicinchoninic acid (BCA) protein assay (Thermofisher Scientific, USA), and samples were aliquoted and stored at −80°C until further use ...
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bioRxiv - Cell Biology 2022Quote: Total proteins were measured in the serum of the toads using a BCA protein quantification kit (Thermo Fisher, Cat 23225).
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bioRxiv - Molecular Biology 2022Quote: ... The lysed supernatant was obtained by centrifugation at 4°C for 10 min at 13,000 rpm and the total protein content was quantified by a Micro BCA Protein assay kit (Thermo Scientific). The proteins were boiled in Laemmli SDS sample buffer (Alfa Aesar ...
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bioRxiv - Physiology 2022Quote: Protein concentration of cell lysates was determined using the PierceTM BCA protein assay kit according to manufacturer’s instructions (ThermoFisher Scientific). Samples were subsequently subjected to SDS-PAGE and blotted on a nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2022Quote: ... Total protein concentration was subsequently determined using the fluorescence-based EZQ™ Protein Quantitation Kit (Life Technologies, Carlsbad, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... The concentration of the protein was determined using the Pierce BCA Protein Assay Kit (cat. No. 23225; Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2022Quote: ... Extracted protein from the cell-only control samples was quantified using Pierce Rapid Gold BCA Protein Assay Kit (Thermo Fisher). 10 µg protein from each sample was then analyzed by sodium dodecyl sulfate– polyacrylamide gel electrophoresis (SDS-PAGE ...
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Mechanism of the ANT-mediated transport of fatty acid anions across the inner mitochondrial membranebioRxiv - Biophysics 2022Quote: The protein concentration of the proteoliposomes was measured with the Micro BCATM Protein Assay Kit (Thermo Fisher Scientific, Prod. #23235). SDS-PAGE and silver staining verified protein purity ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell debris were pelleted by centrifugation (14,000g, 10min, 4°C) and whole cell protein extract concentration was measured using Qubit Protein Assay Kit (Thermo Fisher). Protein extracts were immediately used for EnzyChrom Catalase Assay Kit and EnzyChrom Glutathione Reductase Assay Kit (BioAssay Systems) ...
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bioRxiv - Developmental Biology 2022Quote: Protein synthesis was detected by the Click-iT® Plus OPP Alexa Fluor 594 Protein Synthesis Assay Kit (Molecular Probes) as previously described (Sanchez et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration was measured with BCA Protein Assay Kit according to the manufacturer’s instructions (Thermo Fisher Scientific, Carlsbad, CA, USA). Then ...
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bioRxiv - Cell Biology 2022Quote: Surface proteins on Caco-2 cells were biotinylated using Pierce Cell Surface protein biotinylation and Isolation Kit (Thermo Scientific, A44390) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Total protein concentration in each sample was quantified using Pierce 660nM protein assay reagent (Thermo Fisher Scientific, Woolwich, UK, #22662) supplemented with ionic detergent compatibility reagent (IDCR ...
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bioRxiv - Cell Biology 2022Quote: Protein complexes from the eluate were resolved by SDS-PAGE and stained with Coomassie blue (Imperial Protein Stain, ThermoFisher Scientific). Protein bands of interest between 250 kDa and 20 kDa were excised ...
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bioRxiv - Neuroscience 2021Quote: ... an aliquot of the lysate was retained to measure the total protein content and the remainder was precleared by adding 20 μl of 50% protein G-agarose (PGA, Invitrogen) in coIP buffer for 30 min at 4 °C with rotation ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentrations were determined using a Pierce BCA Protein Assay kit as instructed by the manufacturer (Thermo Fisher; Waltham, MA).
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bioRxiv - Microbiology 2021Quote: ... the supernatant was collected and the protein concentration was determined with a BCA protein assay kit (Thermo Fisher Scientific, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Protein samples were heated to 70°C and loaded on 3-8% Tris-Acetate Protein Gels (ThermoFisher Scientific, Waltham, MA) or NuPAGE 4-12% Bis-Tris Protein Gels (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... A mouse monoclonal anti-HSV 45-kDa protein (scaffolding protein) antibody (Serotec) at 1:2000 dilution and Alexa Fluor 568–goat anti-mouse IgG (Invitrogen) at 1:1000 dilution were used as primary and secondary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... according to the manufacturer’s protocol and normalized to total protein concentrations using the Pierce BCA Protein Assay kit (Thermo Scientific).
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bioRxiv - Cell Biology 2021Quote: ... The protein concentration of clarified cell lysate was then measured using the BCA protein assay kit (Thermo Fisher, Cat# 23227). 5 ∼ 20 μg of total protein per lane was separated by SDS-PAGE gel and transferred to PVDF membrane ...
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bioRxiv - Biochemistry 2021Quote: ... Protein concentrations were determined by Bradford assay and equal amounts of protein were added to a slurry of Dynabeads Protein G (ThermoFisher) and p300 antibody (NM11 ...
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bioRxiv - Neuroscience 2020Quote: ... protein concentration in each sample was determined by using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, 23225). 75µg protein per condition was transferred into new microcentrifuge tubes and 5 µL of the 200 mM TCEP was added to reduce the cysteine residues and the samples were then incubated at 55°C for 1-h ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein-containing supernatants were pre-cleared for 1 h at 4°C with Dynabead® protein G (Thermo Fisher Scientific). Pre-cleared supernatants were incubated overnight at 4°C with 1:1250 dilution of Rabbit (DAIE ...
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bioRxiv - Physiology 2021Quote: ... centrifuged for 1 min at 10’000 x g and 40 μg protein was loaded onto NuPAGE Bis-Tris 10% Protein Gels (ThermoFisher #NP0301BOX), and proteins were transferred to nitrocellulose membranes (Sigma #GE10600002) ...
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bioRxiv - Microbiology 2022Quote: ... trichloroacetic acid was performed as described previously 37 and protein concentration was determined by BCA protein assay kit (Thermo scientific). Protein samples were separated by 4– 12% gradient SDS-PAGE (GenScript ...
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bioRxiv - Developmental Biology 2022Quote: ... ex vivo: 20 μg) of protein lysates as described above were resolved on 4- 12% Bis-Tris Protein gels (Invitrogen). Proteins were transferred to nitrocellulose membranes (Millipore ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... AChE activity was normalized by protein concentration as determined by a Coomassie (Bradford) protein assay kit (Thermo Scientific, Waltham, MA) and compared to 0.5% DMSO exposed samples (set at 100% activity) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... ChE activity was normalized by protein concentration as determined by a Coomassie (Bradford) protein assay kit (Thermo Scientific, Waltham, MA) and compared to vehicle-exposed samples (set at 100% activity) ...
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bioRxiv - Cell Biology 2022Quote: ... Molecular weight markers used were Novex Sharp Pre-stained Protein Standard (LC5800) or PageRuler Prestained Protein Ladder (26617) (ThermoFisher Scientific). Following SDS-PAGE ...
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bioRxiv - Cell Biology 2022Quote: ... Protein purity was determined by resolving purified protein by SDS–PAGE using NuPAGE 4–12% Bis Tris precast gels (ThermoFisher) at 200 V ...
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bioRxiv - Neuroscience 2019Quote: Whole cell protein lysates were isolated from hippocampal tissues by using M-PER mammalian protein extraction reagent (Thermo Scientific, 78501) and by adding Halt Protease Inhibitor (Thermo Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... Cell-surface proteins were purified using Mem-PER™ Plus Membrane Protein Extraction Kit (Thermo Fisher Scientific, Rockford, lL, USA). Isolated proteins were labeled with Cy3 dye and hybridized to a lectin microarray 27–35 ...
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bioRxiv - Molecular Biology 2019Quote: ... Exosomes were used in co-culture assay and also exosomal RNA and protein were extracted using the Total Exosome RNA and Protein Isolation kit (ThermoFisher) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2019Quote: The protein lysate or exosomal protein from of WT H69 and ∆huPGRN-H69 cells were prepared using M-PER mammalian protein extraction reagent (ThermoFisher) or exosome protein isolation kit (ThermoFisher ...
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bioRxiv - Bioengineering 2019Quote: ... RLU readings were normalized to total protein content as measured by Pierce BCA Protein Assay Kit (ThermoFisher Scientific; Waltham, MA). Vector genome copies were measured by qPCR (Supplementary Methods).
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bioRxiv - Microbiology 2019Quote: ... The protein concentrations of the lysates were then quantified using a bicinchoninic acid protein kit (Thermo Fisher Scientific, Rockford, IL). Aliquots containing 12 µg of total cellular lysates for each sample were denatured at 100 °C for 5 min and separated using 8% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Physiology 2019Quote: ... The protein concentration was measured with the reducing agent compatible Pierce BCA Protein Assay Kit (Thermo Scientific, Rockford, IL, USA).
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bioRxiv - Cancer Biology 2019Quote: ... Total protein in the CM was estimated using the Pierce™ BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Cancer Biology 2019Quote: ... Supernatants were collected and total protein concentration was measured using a BCA Protein Assay Kit (Thermo Scientific, Waltham, MA, USA). An equimolar amount of protein was loaded into each well and separated by 12% SDS-PAGE ...