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Citations for Thermo Fisher :
7701 - 7750 of 10000+ citations for West Nile Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Lysates were clarified and proteins were immunoprecipitated using α-FLAG M2 magnetic beads or protein G-conjugated Dynabeads (ThermoFisher) pre-incubated with rabbit α-GFP antibody (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... The EV and cell lysate protein concentrations were determined using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). The protein lysates were separated using Bolt 4–12% Bis-Tris Plus Gels (Burlington ...
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bioRxiv - Synthetic Biology 2022Quote: ... The content of protein in the samples was determined with the A280 Total Protein Assay (Thermo Scientific, Wilmington, DE). A volume equivalent to 100 μg protein per sample was taken through a modified filter-aided sample preparation (FASP ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentrations of the EV isolates were determined using the PierceTM BCA protein assay kit (Thermo Fisher Scientific) and 10 ug aliquots were prepared and stored at -80°C until further analysis.
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration of the supernatant was then determined using Pierce™ BCA Protein Assay Kit (# PI23225, Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Protein concentrations of all samples were determined using the Pierce Bicinchoninic Acid (BCA) Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Immunology 2023Quote: ... Results were normalized by total protein concentrations as determined by Pierce BCA Protein Assay Kit (ThermoFisher Scientific, catalog #23225). PBMC lysates were not diluted for this assay ...
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bioRxiv - Cancer Biology 2023Quote: Bicinchoninic acid (BCA) assay protein concentration determination: The Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, cat no. 23225) was used for protein quantification ...
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bioRxiv - Plant Biology 2023Quote: ... Total protein content in the supernatant was estimated using the Pierce BCA protein assay kit (Thermo Scientific, Rockford, IL). The protein samples were denatured at 65 °C in the 1X LDS sample buffer (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: Lysates containing 25 µg of protein were electrophoresed on a NuPage 4%-12% Bis-Tris Protein gel (Thermofisher Scientific). Proteins were transferred to a PVDF membrane (0.2 µm ...
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bioRxiv - Physiology 2023Quote: ... Protein concentrations were determined with a BCA assay (Pierce™ BCA Protein Assay Kit; #23225, ThermoFisher, Carlsbad, CA, USA). Total protein (5∼15 µg ...
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bioRxiv - Biochemistry 2023Quote: ... Purified and concentrated EGFP_Nanobody protein was immobilized onto agarose beads (20 mg protein per column) using AminoLink Plus Immobilization Kit (ThermoFisher), following manufacturer’s instructions in PBS pH 7.5 coupling buffer.
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bioRxiv - Cancer Biology 2023Quote: ... Protein extraction was performed lysing the cells with M-PER (Mammalian Protein Extraction Reagent, ThermoFisher Scientific, Waltham, MA, USA) lysis buffer plus Halt Protease and Phosphatase Inhibitor Cocktail ...
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bioRxiv - Plant Biology 2023Quote: ... and protein expression was induced by methanol in BMMY medium following the description in Pichia protein expression handbook (Invitrogen). SCP8 protein was purified by affinity chromatography with a His-trap column (Cytiva ...
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bioRxiv - Physiology 2023Quote: ... The respective protein concentration was measured using the PierceTM BCA Protein Assay Kit (Thermo Fisher Scientific; Waltham, MA, USA). Mitochondria-rich fraction (10 µg of protein ...
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bioRxiv - Biochemistry 2023Quote: ... The protein concentration of each AF sample was measured using a Pierce BCA Protein Assay kit (Thermo scientific 23227) and then normalized to equal concentrations for downstream analysis.
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bioRxiv - Cell Biology 2023Quote: ... and protein concentration of the supernatant was determined by Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific; 23225). Spastin protein was resolved on 8% SDS-PAGE gels transferred to Immobilon®-FL PVDF membranes (Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... while the protein content was measured using the Pierce™ BCA Protein Assay Kit (catalog no: 23225, ThermoFisher Scientific), following manufacturers’ instructions.
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bioRxiv - Plant Biology 2023Quote: ... Protein abundance was determined using the protein area calculated by the Proteome Discoverer software (Thermo Fisher Scientific; version 1.4).
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bioRxiv - Molecular Biology 2023Quote: ... Equivalent protein quantities for all genotypes were loaded onto a Nupage 4-12% Bis-Tris Protein Gels (ThermoFisher Scientific) for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... Remaining protein pellets were dissolved with 8M urea and total protein was quantified using Pierce BCA assay (Thermo Fisher). Total protein amount was used for equivalent loading for High Performance Liquid Chromatography and High-Resolution Mass Spectrometry and Tandem Mass Spectrometry (HPLC-MS/MS ...
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bioRxiv - Immunology 2023Quote: ... Remaining protein pellets were dissolved with 8M urea and total protein was quantified using Pierce BCA assay (Thermo Fisher). Total protein amount was used for equivalent loading for High Performance Liquid Chromatography and High-Resolution Mass Spectrometry and Tandem Mass Spectrometry (HPLC-MS/MS ...
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bioRxiv - Systems Biology 2023Quote: ... Protein concentrations in the pellet were determined using a Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, MA). After mixing 400 μL of supernatant with 400 μL of chloroform and 320 μL of water ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were clarified by centrifugation at 4°C and the protein concentration was determined by BCA protein assay (Thermofisher). Samples were resolved on a precast 4%–12% gradient gel (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein content in the supernatant was determined using a colorimetric micro–BCA Protein Assay kit (Thermo Fisher Scientific).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Total protein served as the loading control by covalently labeling lysine residues (No-Stain Protein Labeling Reagent, Thermo Fisher) following the manufacturer’s protocol and visualized by fluorescence imaging (488 nm) ...
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bioRxiv - Genetics 2023Quote: ... we incubated 2-5μg sheared DNA with equal volume of DynabeadsTM Protein A and Protein G (ThermoFisher Scientific Inc.). ChIP-seq libraries were prepared with a NEBNext DNA Ultra II kit following manufacturer recommendations ...
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bioRxiv - Developmental Biology 2023Quote: ... using the Qbit 4 fluorometer (with the Broad range protein assay kit Qubit Protein BR Assay, from ThermoFisher Scientific), and sugar concentration using the refractometer (refractometer RBR32-ATC) ...
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bioRxiv - Cancer Biology 2023Quote: ... The ladders used were Novex Sharp Pre-Stained Protein Standard and SeeBlue Plus2 Pre-Stained Protein Standard (Life Technologies). The gels were transferred on to a 0.2µm nitrocellulose membrane (Thermo-Scientific ...
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Comparative Proteomic Study of Retinal Ganglion Cells Undergoing Various Types of Cellular StressorsbioRxiv - Molecular Biology 2023Quote: ... Proteins from isolated RGCs were extracted using T-PER™ Mammalian Protein Extraction Reagent (Thermo Fisher Scientific, Cat.# 78510) supplemented with HALT protease inhibitor cocktail (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... 6000 rpm 4°C and protein concentration was estimated by BCA protein assay kits (Thermo-Fisher Scientific, Cat #23225).
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bioRxiv - Bioengineering 2024Quote: Protein samples were diluted in PBS to 5 μM and mixed with 5000× SYPRO Orange Protein Gel Stain (Invitrogen) to final 1.25x concentration ...
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bioRxiv - Microbiology 2024Quote: ... The concentrations of the protein extracts were determined using a BCA assay (Pierce BCA Protein Assay, Thermo Fisher Scientific) and a verification of the protein extracts was carried out on a 12.5% acrylamide SDS-PAGE gel stained with Coomassie brilliant blue R-250 ...
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bioRxiv - Microbiology 2024Quote: ... The concentration of the protein was estimated using the BCA kit (Pierce BCA Protein Assay Kit - Thermo Fisher Scientific) according to the supplier’s recommendations as well as by comparing the protein on a 12.5% acrylamide Coomassie-stained SDS-PAGE gel with BSA standards ...
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bioRxiv - Synthetic Biology 2024Quote: ... Total protein content was quantified using PierceTM BCA protein assay kit as per the supplier’s protocol (Thermo Scientific, USA). For albumin ...
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bioRxiv - Molecular Biology 2024Quote: ... 15 µl (for ChIP) or 100 μl (for ChIP-seq) Dynabeads Protein A and Protein G (Thermo Fisher Scientific) were pre-incubated overnight with rotation in the presence of 5 mg/ml BSA and 3 µg (for ChIP ...
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bioRxiv - Cell Biology 2024Quote: ... The clarified protein samples were incubated with NeutrAvidin® Agarose resin (50 µL per 1 mg protein, Thermo Scientific) for 2 hours at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Supernatants were collected and the total amount of proteins was calculated by the BCA Protein Assay kit (ThermoFisher Scientific) using a BSA standard curve ...
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bioRxiv - Cell Biology 2024Quote: ... Peak measurements were normalized to protein content of duplicate samples as determined by BCA protein assay (Thermo Fisher Scientific). All experiments were performed independently at least three times and a representative experiment is shown.
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bioRxiv - Neuroscience 2024Quote: ... the samples underwent normalization based on protein content using the BCA protein assay kit (#23225, Thermo Scientific Pierce, US) per the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... The total protein concentration of each cell lysate was determined with a BCA protein assay kit (Thermo Fisher, # 23225). For each sample ...
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bioRxiv - Biochemistry 2024Quote: Protein in mouse and human wound fluid samples were quantified with the BCA Protein Assay kit (Thermo Fisher Pierce) using the 96-well plate protocol recommended by the manufacturer ...
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bioRxiv - Biochemistry 2024Quote: ... and total protein concentrations of cleared cell lysates were measured with the PierceTM BCA Protein Assay Kit (ThermoFisher Scientific) using BSA as a standard ...
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bioRxiv - Cell Biology 2024Quote: ... and total protein concentrations were quantified using a Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA). Samples for gel electrophoresis were prepared by boiling in loading buffer for 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... Total protein levels in all lysates were quantified using PierceTM BCA Protein Assay Reagents (Thermo Fisher: #23222 and #23224). After the incubation step for the poly-GP immunoassay ...
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bioRxiv - Microbiology 2024Quote: ... The protein concentration in each sample was determined using the BCA protein assay kit (Thermo Fisher Scientific, Waltham, USA) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and the resulting supernatant was assayed for protein concentration with a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) and then subjected to SDS-polyacrylamide gel electrophoresis on a 10% acrylamide/4% bisacrylamide gel ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysate was clarified by centrifugation and total protein concentration was measured with the BCA Protein Assay (Thermo Fisher Scientific). Samples were electrophoresed and transferred to a 0.45 μm nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Systems Biology 2024Quote: ... The protein concentrations in both supernatants (SOL and SDS) were determined with Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Cell Biology 2024Quote: Protein concentration in the samples was determined with a BCA assay (Pierce BCA Protein Assay Kit; Thermo Fisher Scientific) according to the manufacturer’s instructions ...