Labshake search
Citations for Thermo Fisher :
8001 - 8050 of 10000+ citations for West Nile Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2023Quote: ... Lysate inputs were normalized by determining overall protein concentration using Pierce™ BCA Protein Assay (Thermo Fisher Scientific, Waltham, MA). Subsequently ...
-
bioRxiv - Cell Biology 2023Quote: ... 1% of input chromatin was saved and 70 μg of chromatin pre-clearing was done by mixing with pre-blocked Sepharose beads (1:1 vol. mix of Protein A and protein G beads; Invitrogen) for 1h at 4°C ...
-
bioRxiv - Microbiology 2023Quote: ... The protein content of the samples was quantified using the Pierce™ 660 nm protein assay kit (Thermo Fisher, US) with BSA as the standard ...
-
bioRxiv - Cell Biology 2023Quote: ... 0.5 M sucrose) and a protein concentration assay was performed (BCA Protein Assay Kit, Pierce-Thermo Fisher Scientific, Melbourne, Australia). 100 μg of isolated mitochondria were collected and centrifuged at 12,000 g for 15 min at 4 °C and after removal of the supernatant the pellet was frozen at −80 °C for subsequent proteomics analysis.
-
bioRxiv - Cancer Biology 2023Quote: ... Proteins were quantified via a BCA assay using the Pierce BCA Protein Assay Kit obtained from Life Technologies (Catalog #23225). Cell lysates were run through a 4-12% SDS-PAGE gel electrophoresis at 140-170V ...
-
bioRxiv - Neuroscience 2023Quote: ... 14.000 RPM) and protein concentration of collected supernatants measured using the Pierce BCA Protein Assay Kit (#323227, Thermo Scientific, 23227). The samples adjusted to contain equal amounts of protein (10 μg or 12 μg ...
-
bioRxiv - Microbiology 2024Quote: ... The supernatants (1.2 mL) were subjected to immunoprecipitation with 30 μG of protein G magnetic beads (Dynabeads Protein G, Thermo Scientific) overnight ...
-
bioRxiv - Neuroscience 2024Quote: Protein samples prepared from heads of 10-20 males and females in protein extraction buffer plus 1% protease inhibitor (ThermoFisher) and 1% phosphatase inhibitor (Sigma ...
-
bioRxiv - Neuroscience 2024Quote: ... Protein concentration in the tissue lysates were determined by Pierce™ BCA protein assay kit (Thermo Scientific, Cat# 23227, USA). Protein lysates were suspended in sample buffer containing NuPAGE™ LDS Sample Buffer (Invitrogen™ ...
-
bioRxiv - Neuroscience 2024Quote: ... prior to proceeding with protein quantification using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, cat. no. 23225). 10µg of proteins were resolved using SDS-PAGE (4-12% Bis-Tris precast gels in buffer MES) ...
-
bioRxiv - Cell Biology 2024Quote: ... The 20 μg of extracted proteins were quantified using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, WA, USA) at 500 ng/μL ...
-
bioRxiv - Neuroscience 2024Quote: ... with 2 µl of supernatant per sample used to test protein concentration (Pierce 660 nm Protein Assay, Invitrogen, Cat: 22662). Samples were stored in a -20°C freezer prior to LC/MS-MS ...
-
bioRxiv - Immunology 2024Quote: Purified recombinant protein vaccines were analyzed on SDS-PAGE by staining with GelCode Blue Safe Protein Stain (Thermo Fisher Scientific). Proteins separated by SDS-PAGE were also transferred onto nitrocellulose membranes for immunoblotting ...
-
bioRxiv - Immunology 2024Quote: ... 0.3 µg of each gRNA was mixed with 2 µg Cas9 protein (TrueCut™ Cas9 Protein v2, Thermo Fisher Scientific) in 5 µl E8 medium supplemented with 10 μM Y27632 ...
-
bioRxiv - Microbiology 2024Quote: ... The total protein content in the supernatant fraction was determined by Pierce™ BCA protein assay kit (Thermo Fisher Scientific). An equivalent amount of total proteins was loaded in each lane and resolved on a 15% SDS-PAGE gel or 4-12% SurePAGE™ Bis-Tris gel (Genscript) ...
-
bioRxiv - Cell Biology 2024Quote: ... Eluates were mixed with protein sample buffer for SDS-PAGE and analyzed with SYPRO Ruby protein gel stain (ThermoFisher S12000), autoradiography or western blotting.
-
bioRxiv - Microbiology 2024Quote: The protein concentration of gradient fractions 6 and 17 was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher). Ten micrograms of the purified fractions 6 (exosome fraction ...
-
bioRxiv - Neuroscience 2024Quote: Total protein levels were quantified in tissue homogenates in triplicates using the Pierce Microplate BCA Protein Assay Kit (Thermo Scientific Pierce Protein Biology ...
-
bioRxiv - Physiology 2024Quote: ... The total islet protein content for each secretion assay was measured with Micro BCA™ Protein Assay Kit (ThermoFisher, #23235). Data represent the glucagon or insulin content per µg of protein in each tube.
-
bioRxiv - Systems Biology 2024Quote: ... Lysates were centrifuged for 10 min at 14.000 rpm and 4°C followed by protein determination with PierceTM BCA Protein Assay Kit (Thermo Scientific) according to manufacture description ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... and the protein concentration in the supernatant was determined using a BCA Protein Assay Kit (Cat# 23225, Thermo Fisher Scientific). Afterward ...
-
bioRxiv - Cell Biology 2024Quote: ... Supernatants were transferred to new tubes and protein concentrations were determined using the BCA Protein Assay Kit (Thermo Fisher Scientific). Between 20-50 μg of protein per sample was mixed with Laemmli sample buffer and boiled for 10 min ...
-
bioRxiv - Neuroscience 2024Quote: ... protein concentrations were quantified using a Bicinchoninic acid assay (BCA) with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). The lysates were diluted to a concentration of 1.5 μg/μL and stored at -80°C until analyzed by Western blot.
-
bioRxiv - Molecular Biology 2024Quote: ... Supernatants were transferred to new tubes and protein concentrations were determined by BCA Protein Assay Kit (Thermo Fisher Scientific, 23225). Between 20-50 μg of protein per sample was mixed with Laemmli sample buffer (62.5 mM Tris-HCl (pH 6.8) ...
-
bioRxiv - Plant Biology 2020Quote: ... Dynabeads Protein A or G (Thermo Fisher Scientific) were used ...
-
bioRxiv - Plant Biology 2020Quote: ... 20 μl of slurry Protein G beads (Invitrogen) per gram of tissue were added and subjected to a further incubation for 2 hours with rotation at 4°C ...
-
bioRxiv - Plant Biology 2019Quote: ... were used with Dynabeads Protein G (Thermo Scientific). Protein– DNA complexes were eluted from the beads as described in (Rowley ...
-
bioRxiv - Genetics 2021Quote: ... Pierce™ BCA protein assay kit (Thermo Fisher) was used to quantify the protein levels in the supernatant ...
-
bioRxiv - Biochemistry 2021Quote: ... Expi293F cells used for protein expression (ThermoFisher Scientific) were cultured in Expi293 media (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2021Quote: ... and Dynabeads Protein G (ThermoFisher SCIENTIFIC, Cat #10004D). RNA was partially digested with RNase I (ThermoFisher SCIENTIFIC ...
-
bioRxiv - Cancer Biology 2021Quote: ... Coomassie Protein Assay Reagent kit (Thermo Fisher Scientific) was used to measure protein concentration ...
-
bioRxiv - Cancer Biology 2021Quote: ... Wash the protein G magnetic beads (Invitrogen, 10612D) with ripa 0.3 buffer ...
-
bioRxiv - Molecular Biology 2020Quote: ... 42 μg of Dynabeads-Protein G (ThermoFisher Scientific), pre-washed overnight in 10 volumes of IP buffer (40 mM HEPES pH 7.4 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Protein concentration was determined by BCA (Thermo Scientific) assay ...
-
bioRxiv - Cell Biology 2020Quote: ... Bicinchoninic acid (BCA) protein assay (Thermo Fisher Scientific) was performed to determine protein concentration ...
-
bioRxiv - Cell Biology 2020Quote: ... protein concentration was determined using BCA reagent (ThermoFisher). protein concentration was around 25 µg/mL per sample ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were digested with trypsin (Thermo Fisher Scientific) at a 1:50 trypsin:protein ratio at 37 °C overnight ...
-
bioRxiv - Cell Biology 2020Quote: ... pre-washed dynabeads coupled to protein G (Invitrogen) were added to the lysate and the mixture incubated at 4°C for 4 hours ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein concentration was estimated using NanoDrop 1000 (ThermoFisher) and was used immediately for malachite green phosphatase assays.
-
bioRxiv - Cell Biology 2020Quote: ... PageRuler Plus Prestained Protein Ladder (Thermo Fisher Scientific) was used to estimate the molecular size of bands ...
-
bioRxiv - Cell Biology 2020Quote: Protein depletion was achieved using Stealth siRNAs (Invitrogen). The following oligos were used ...
-
bioRxiv - Cell Biology 2020Quote: ... Magnetic beads (Dynabeads Protein A or G, Invitrogen) preloaded with an anti-HA antibody were added to the supernatant and incubated at 4°C for 4h ...
-
bioRxiv - Cell Biology 2020Quote: ... quantified by Pierce BCA Protein Assay (Thermo Scientific) were mixed with 5x SDS sample buffer (312.5 mM Tris,10 % SDS ...
-
bioRxiv - Immunology 2021Quote: ... 1.0 mm Mini Protein Gel (Thermo Fisher Scientific). Briefly ...
-
bioRxiv - Genetics 2021Quote: ... Protein A Dynabeads (15 μl; Invitrogen, cat. # 1002D) were incubated in blocking solution (0.5% bovine serum albumin in PBS ...
-
bioRxiv - Developmental Biology 2021Quote: ... Protein expression was induced by adding IPTG (Invitrogen) to the culture to a final concentration of 0.1mM and shaken at 28°C for approx ...
-
bioRxiv - Genetics 2020Quote: ... 50 µL magnetic beads (Protein G Dynabeads, Invitrogen) were pre-blocked in fresh blocking buffer (0.5% BSA in PBS ...
-
bioRxiv - Immunology 2020Quote: ... Twenty five μL of G-protein dynabeads (Invitrogen) were added for rotation for 4 h at 4°C ...
-
bioRxiv - Developmental Biology 2021Quote: ... A BCA protein assay kit (Thermo Scientific, #23225) was used to determine protein content of the supernatant fractions and 300 µg of total protein was used for the following steps ...
-
bioRxiv - Molecular Biology 2022Quote: ... and 50ul Protein-G magnetic beads (Thermo Scientific) at 4 Celsius for 2 hours ...