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Citations for Thermo Fisher :
7801 - 7850 of 7943 citations for Recombinant Human Leptin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... cells were incubated for 1 hour sat room temperature with the following secondary antibodies: anti-goat anti-human Alexa Fluor 647 (Invitrogen, Carlsbad, California, USA). All washing steps were performed with PHEM-T buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then handled by the University of Colorado Anschutz Genomics and Microarray Core for microarray analysis using a Human Clariom D chip (902922; Applied Biosystems via ThermoFisher). This array has over 540,000 transcripts on it including mRNAs ...
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bioRxiv - Cell Biology 2024Quote: ... The hTERT RPE-1 human epithelial cell line (ATCC-CRL4000) was obtained from ATCC and grown in DMEM/F12 (ThermoFisher Scientific, Carlsbad, CA) with 10% fetal bovine serum (FBS) ...
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bioRxiv - Developmental Biology 2024Quote: ... 5% CO2, and 95% humidity. GFP labeled Human Brain Vascular Pericytes (HBVP) (ScienceCell™, Cat. #: 1200) were grown in DMEM (Gibco, Cat. # 11995065) supplemented with 10% FBS and 1X Antibiotic-Antimycotic under the cell culture conditions indicated above ...
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bioRxiv - Cell Biology 2024Quote: ... Human embryonic kidney (HEK) 293T cells were from ATCC (cat. #CRL-3216) and were cultured in high-glucose DMEM (Gibco, cat. #41966-052) supplemented with 10% FBS and 1% Penicillin-Streptomycin.
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bioRxiv - Cancer Biology 2021Quote: ... in CD-1 mouse, Sprague−Dawley rat, and human plasma (Bioreclamation, Inc., Hicksville, NY) by equilibrium dialysis using the RED Device (Thermo Fisher Scientific, Rockford, IL). Compounds were added to pooled plasma (n ≥ 3 ...
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bioRxiv - Developmental Biology 2021Quote: ... the causal relation that a target gene is up- or downregulated depending on the transcription complex being active or inactive and (ii) the causal relation that a signal from a gene expression microarray (e.g. Affymetrix Human Genome U133 Plus 2.0) probeset is high or low depending on the target gene being up or down ...
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bioRxiv - Cell Biology 2020Quote: ... α-SC35 (described previously98, Mouse 1:1000), α-NIN (Institut curie, Human 1:200), anti-Arl13b (proteintech, Rabbit, 1:4500) α-Rabbit Alexa594 (ThermoFisher, 1:400), α-Mouse IgG1 Alexa488 (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: ... In a second set of experiments Human HK-2 cells were cultured in DMEM and Keratinocyte-SFM (1X) medium (Life Technologies Green Island NY). When the cells reached 70% confluence ...
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bioRxiv - Cell Biology 2020Quote: Total RNA extracted from EndoC-βH1 and collagenase-isolated human pancreatic islets samples was quantified using Qubit 3000 Fluorometer (ThermoFisher Scientific, Waltham, MA, USA), while those extracted from LCM-islets were quantified using 2100 Bioanalyzer-RNA 6000 Pico Kit (cat ...
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bioRxiv - Developmental Biology 2021Quote: ... For cells freshly purifed from human lung tissues were directly lysed using 100 μl lysis buffer from PicoPure™ RNA Isolation Kit (Thermo Fisher Scientific, KIT0204). First Strand cDNA synthesis was performed using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... according to manufacturer’s protocol Following ChIP-valuated antibodies were used for precipitation: mouse-anti-human H3K9/14/18/23/27ac (Thermo Fisher Scientific, Carlsbad, CA, USA) and mouse-anti-IgG (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Blots were probed with primary antibodies: human anti-SARS-CoV-2 Spike (S-ECD/RBD) (cat# bcb03, Thermo Fisher, 1:1000, non-reducing conditions), anti-Salmonella typhimurium LPS (cat# ab8274 ...
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bioRxiv - Molecular Biology 2020Quote: ... falciparum asexual blood stages were cultured in human erythrocytes (National Blood Transfusion Service, London, United Kingdom) and complete medium (CM) consisting of RPMI-1640 medium (Life Technologies, California, United States) supplemented with 0.5% AlbuMAX type II (Gibco) ...
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bioRxiv - Genomics 2020Quote: ... Human DNA was removed using DNase I and RNA concentration was measured using Qubit RNA HS Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). DNA-depleted and purified RNA was used to construct double-stranded DNA library using MGIEasy RNA Library preparation reagent set (MGI ...
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bioRxiv - Genetics 2019Quote: ... chronic myelogenous leukemia cells and six independent transfections in SH-SY5Y (ATCC#CRL-2266) human neuroblastoma cells in two batches by lipofectamine3000 (Thermofisher Scientific cat. no L3000008). We then extracted DNA and RNA and performed 50 bp non-paired end sequencing reading from the 3’end ...
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bioRxiv - Biophysics 2021Quote: ... of Plasmodium falciparum plasmepsin X (XP_001349441) (Fig. 5a) was engineered for expression in a mammalian host (Human Embryonic Kidney cells, HEK cells) by GeneArt (Thermo Fisher, Waltham, MA, USA) and cloned into a variant of pTT5 with the mouse Ig secretion signal peptide on the N terminus and a TEV-cleavable 8x Histidine tag on the C terminus ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then incubated for 48h at 4°C in the same solution with mouse anti-NeuN (1:2000; Chemicon; MAB377, Temecula, CA, USA) and anti-human PHF-Tau antibody clone AT8 (1:2000, MN1020, Thermo Scientific, Waltham, MA, USA); for the sake of clarity ...
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bioRxiv - Cancer Biology 2020Quote: Human CD34+ cells were isolated from patient bone marrow samples by erythrocyte lysis buffer treatment, followed by separation with AutoMACs sorter and CD34 Microbead Kit human (MiltenyiBiotech, Germany) and grown in IMDM (Gibco, Thermo Fisher Scientific, CA, USA) supplemented with 2% FBS (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... The iPSCs were cultured on irrMEF with medium for human iPSCs : KnockoutTM-Dulbecco’s modified Eagle’s medium (KO-DMEM: 10829-018, Life Technologies; Thermo Fisher Scientific, MA, USA) supplemented with 20% KnockoutTM-Serum Replacement (KO-SR ...
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bioRxiv - Immunology 2020Quote: ... the plates were washed 5 times and incubated with goat anti-human IgG (H+L) antibody conjugated with ALP (Thermo Fisher, a18808, 1:5000) for 30 min at 37 °C ...
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... The fluorescent secondary antibodies used in this study were Alexa Fluor 568 goat anti-human IgG(H+L) (Molecular Probes, A-21090, 1:1000), Alexa Fluor 488 goat anti-mouse IgG(H+L ...
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bioRxiv - Cancer Biology 2020Quote: Peptide spectrum matching of MS/MS spectra of each file was searched against the human database (UniProt) using the Sequest algorithm within Proteome Discoverer v 2.3 software (Thermo Fisher Scientific, San Jose, CA). The Sequest database search was performed with the following parameters ...
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bioRxiv - Cell Biology 2022Quote: Plasmodium falciparum 3D7 strain parasites were cultured within human O+ erythrocytes maintaining 4% haematocrit in RPMI 1640 medium (Invitrogen Corp., San Diego, CA, USA) supplemented with Albumax I (0.5% ...
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bioRxiv - Biochemistry 2020Quote: ... The human Bip 19-654aa (referred to as Bip)-encoding DNA was cloned into a modified pProEx™-HTb vector (Thermo Fisher Scientific Inc.), resulting in a construct with a N-terminal 6xHis tag ...
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bioRxiv - Microbiology 2021Quote: ... A donkey anti-mouse IgG-Alexa Fluor 555 antibody (SG2-2E6) or goat anti-human IgG-Alexa Fluor 488 antibody (93k) (Life Technologies, Grand Island, NY) was used to detect bound anti-VZV gB mAb ...
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bioRxiv - Molecular Biology 2020Quote: ... driven by the human cytomegalovirus (CMV) immediate early enhancer and promoter was assembled using the GeneArt CRISPR Nuclease Vector system (Thermo Fisher Scientific, Waltham, MA), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: After the skin flap surgery and 15 min prior to the tail-vein injection of GFP-expressing bacteria (107 CFU in 100 μl 1x PBS) and PE-conjugated anti-human CD62E (6 μg, Thermofisher Scientific, #12-0627-42), 100 μg Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin ...
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bioRxiv - Microbiology 2021Quote: ... The technical replicates of the retrieved RNA were pooled prior rRNA depletion, which was performed using the human riboPOOL™ (siTOOLsBiotech, Germany) and Streptavidin-coated magnetic beads (Thermo Fisher Scientific, Norway) following the two-step protocol of the manufacturer ...
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bioRxiv - Neuroscience 2021Quote: ... The presence of secreted Fc Fusion protein in the medium was confirmed by immunoblotting with goat anti-human IgG antibody (Invitrogen, A-21433, 1:500).
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bioRxiv - Immunology 2021Quote: ... Peptide spectrum matching of MS/MS spectra of each file was searched against the human database (UniProt) using the Sequest algorithm within Proteome Discoverer v 2.3 software (Thermo Fisher Scientific, San Jose, CA).
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bioRxiv - Immunology 2022Quote: Human monocytes (20 x 106) were lysed in 650 µl of IP Lysis/Wash buffer (Pierce Direct IP Kit, Thermo Fisher Scientific, Cat. #26148) and the supernatants of cell lysates after centrifugation were transferred to columns containing the TBK1 antibody linked to AminoLink Plus Coupling Resin to pull down the proteins interacting with TBK1 using the Pierce Direct IP Kit according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Human primary B cells from adenoids were separated from T cells by rosetting with sheep erythrocytes (purchased from Thermo Scientific Oxoid, cat. no. SR0051D) and purified by Ficoll density gradient centrifugation as described in the previous paragraph ...
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bioRxiv - Biophysics 2020Quote: ... We performed confocal reflectance microscopy on unlabeled fibrin samples (see Fig. 2) and fluorescence imaging (see Fig. 4) on networks polymerized from unlabeled fibrinogen and Alexa488-labeled human fibrinogen (Life Technologies, Bleiswijk, the Netherlands) mixed in a 30:1 molar ratio.
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bioRxiv - Microbiology 2021Quote: ... HeLa cells (human cervical ATCC CCL-2) and RK13 (Rabbit kidney cells, ATCC CCL37) were grown in Minimum Essential Medium (MEM; Gibco, Thermo Fischer, Lutterworth, UK) supplemented as described above for BSC-1 cells ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed 4X in PBST and subsequently incubated with secondary antibodies (goat anti-rabbit IgG 488, Invitrogen, Carlsbad, CA; #A-11034, 1:4000; & goat anti-human 647, Invitrogen #A-21445, 1:4000) for 1 hour at room temperature ...
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bioRxiv - Genetics 2020Quote: ... Human DNA was removed using DNase I and RNA concentration was measured using Qubit RNA HS Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). After human DNA-depletion ...
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bioRxiv - Developmental Biology 2020Quote: ... the ells were transfected with human EC-SOD (hEC-SOD) cDNA inserted into a vector plasmid pcDNA3 (5446 nucleotides; Invitrogen Life Technologies, Carlsbad, CA, USA) as previously described,14 using the FuGENE kit (Roche Diagnostics ...
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bioRxiv - Biophysics 2020Quote: The plasmid pT7-7 containing human aSyn cDNA was transformed into Escherichia coli One Shot® BL21 (DE3) Star™ (Thermo Fisher Scientific, USA). 0.5 L cultures of E ...
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bioRxiv - Genomics 2021Quote: The pulmonary HLA-DR and CD3-positive cells were isolated using superparamagnetic beads coupled with anti-human CD3 and Pan Mouse IgG antibodies (Invitrogen Dynabeads®, Life Technologies AS, Norway) and HLA-DR/human MHC class II antibodies (Invitrogen Dynabeads® ...
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bioRxiv - Cancer Biology 2021Quote: ... and NCI-H23 cells (human NSCLC) were cultivated at 37°C with 5 % CO2 in RPMI 1640 medium (Gibco, Thermo Fisher Scientific, California, USA) supplemented with 10 % fetal calf serum (FCS ...
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bioRxiv - Immunology 2021Quote: ... Transduction efficiency was determined between days 8-10 of culture using a polyclonal goat anti-human IgG Fc PE (ThermoFisher Scientific, #12-4998-82) and the synaptic transfer to BSLB addressed immediately.
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bioRxiv - Immunology 2021Quote: ... selected human splenic T cells from BLTS-humanized mice and human T cells from PBMCs were seeded at 8×10^4 cells per well (96-well plate) and stimulated with Dynabeads™ Human T-Activator CD3/CD28 (Life Technologies Cat# 11131D) and PBS was used as vehicle control ...
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a Fc part (hinge-CH2-CH3) of human IgG1 for purification was inserted into pFUSE-mIgG1-Fc2 vector (Invitrogen, Thermo Fisher Scientific, MA, USA). The construct (RBD-huFc ...
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bioRxiv - Immunology 2021Quote: Supernatants from 48-hour rested and stimulated iNKT cells and TCONV were assayed for cytokine levels of IFN-γ using human ELISA kit (Invitrogen cat #88-7316) following manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... and then incubated with a 50-fold dilution of PE-conjugated goat anti-human IgM or IgG (both from Thermo Fisher, Waltham, MA, US) for 30 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... The human embryonic kidney 293T/17 cells (ATCC, CRL-11268) were maintained in maintained in Dulbecco modified Eagle medium (Gibco Life Technologies, Gaithersburg, Md.) containing 10% fetal calf serum (FCS) ...
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bioRxiv - Genomics 2021Quote: ... FACS sorted BST2+ T cells and BST2− T cells were stimulated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (ThermoFisher, cat#11161D, USA). Briefly ...
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bioRxiv - Immunology 2022Quote: MACS enriched CD4+ T cells and CD3+ T cells were stimulated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (ThermoFisher, Cat# 11131D, USA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... genes encoding single chain (sc) Fab and scFc linked to human apoferritin light chain monomers were synthesized and cloned by Geneart (Thermo Fisher Scientific, Waltham, MA) into the pcDNA3.4 expression vector ...