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Citations for Thermo Fisher :
7651 - 7700 of 7943 citations for Recombinant Human Leptin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Cells were then washed three times with PBS and incubated with CD15 (C3D-1) mouse anti-human monoclonal antibody (Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells stably expressing the human D1R (D1-HEK) (up to pass 35) were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Gibco/Fisher Scientific, Hampton, NH) supplemented with 10% fetal bovine serum (Omega Scientific ...
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bioRxiv - Immunology 2024Quote: ... The concentration of hIL-1β and hIL-8 were measured using a human Invitrogen ELISA Kit (Thermo Fisher Scientific, Waltham, MA, USA). All the protocols were used according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Cryopreserved primary human hepatocytes were genotyped for PNPLA3 rs738409(G) using TaqMan® SNP Genotyping Assays (Life Technologies, Assay ID C_7241_10, 4351379) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections were transferred to gelatinized slides and immunofluorescence staining was performed using a human CD45 antibody (Rat. YAML501.4; Thermo Fisher Scientific, USA), GFP Polyclonal Antibody (A-11122 ...
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bioRxiv - Immunology 2024Quote: ... highly purified (93-98%) naïve human CD4 T cells were activated using anti-CD3+anti-CD28 Dynabeads (1:1, Thermofisher scientific, cat # 11161D) for 8-24 hours ...
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bioRxiv - Cell Biology 2024Quote: ... one million human iPSCs were thawed and resuspended with 1 mL of Dulbecco’s Modified Eagle Medium F12 (DMEM F12) media (Thermofisher, Catalog No. 12634010) then diluted in 2 mL of warm DMEM F12 in a 15 mL Eppendorf conical tube (Eppendorf ...
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bioRxiv - Cell Biology 2024Quote: The pTP6-3xFlag-KDM6A was obtained through EcoRI digestion and subcloning into the pTP6 vector59 of the 3xFlag-KDM6A sequence optimized for human expression (purchased from Thermo Fisher Scientific). The cDNA encoding for human ZFX was purchased from Genescript ...
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bioRxiv - Cell Biology 2024Quote: Plasmids encoding human ZFP36 proteins TTP and Brf1: human ZFP36 and ZFP36L1 open reading frames were amplified from the ORFeome cDNA clone library (Invitrogen, Life Technologies) by Phusion PCR (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... human MSCs were isolated from the UC (Wharton’s Jelly) and expanded with proliferation medium (Minimum essential Medium-α (MEM-α) (Gibco™) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... The respective mouse and human brain sections were subsequently incubated with Invitrogen donkey anti-rabbit Alexa Fluor 488 (Catalog No. A-21206; Invitrogen, Carlsbad, CA) for VGLUT2 ...
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bioRxiv - Biochemistry 2024Quote: Human embryonic kidney cells (HEK293T, ATCC, #CRL-3216) were cultured in Dulbecco’s modified eagle medium (DMEM, Thermo Fisher Scientific, Invitrogen, Cat#11965092) supplemented with 10% (v/v ...
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bioRxiv - Biochemistry 2024Quote: Human embryonic kidney cells (HEK293T, ATCC, #CRL-3216) were cultured in Dulbecco’s modified eagle medium (DMEM, Thermo Fisher Scientific, Invitrogen, Cat#11965092) supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... The constructs were used for in vitro transcription and translation using the 1-Step Human High-Yield Mini IVT Kit (Thermo Fisher; 88891) according to the manufacturer’s instruction ...
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bioRxiv - Pathology 2024Quote: For routine qPCR analyses 100 mg of grey matter from the STR and the CTX (Brodmann’s area 9/10) of human brains (Table 1) was dissected and extracted in 1.5 ml Trizol reagent (ThermoFisher/Life Technologies, 15596026) using a hand-held homogenizer (6 x15 s bursts)(Pro-Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Human slices were routinely stained with anti-NeuN (diluted 1:300 from stock; Rabbit anti-NeuN, Thermo Fisher Scientific PA5-37407, RRID:AB_2554049), visualized with AF488-conjugated anti-rabbit secondary antibody (1:300 from stock ...
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bioRxiv - Developmental Biology 2024Quote: ... in 2 mL complete human medium (DMEM/F12 [Gibco, Massachusetts, US], 10% FBS [PAA Cell Culture Company, UK], 1% GSP [Gibco, Massachusetts, US]). All cultures were maintained at 37 °C/5% CO2 in a humidified incubator ...
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bioRxiv - Biochemistry 2024Quote: MS/MS searches were carried out using SEQUEST search algorithms against a Uniprot database for humans (supplemented with exogenous sequence for anticipated protein constructs) using Proteome Discoverer (Version 3.0, Thermofisher Scientific, Bremen, Germany). Oxidation of methionine and N-terminal protein acetylation were included as dynamic modifications and carbamidomethylation of cysteine was set as a static modification ...
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bioRxiv - Microbiology 2024Quote: ... Upon stabilisation of baseline readings for human plasma experiments plasma was diluted 1:40 in media (RPMI [Thermo Fisher Scientific, U.S.A.] with B27 Supplement [Thermo Fisher Scientific, U.S.A.]), then transferred to the hiPSC-derived cardiomyocytes for 48 hours of treatment either in the presence or absence of 100ng/mL of dexamethasone (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: Human monocyte-derived macrophages (hMDM) were cultured in Nunc™ MicroWell™ 96-well optical-bottom plates (Thermo Fisher Scientific, Waltham, MA) and left untreated or treated with vehicle (sterile 1X PBS) ...
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bioRxiv - Bioengineering 2024Quote: ... flow throughs and washes from StEVAC method were suspended in 20 µl of Exosome-human CD81 flow detection beads (Thermo Scientific, 10622D) and incubated at 4°C overnight while rotating ...
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bioRxiv - Immunology 2024Quote: ... we co-transfected 3×106 3-day activated CD8+T cells with 150 pmol/106 cells of human TSP-4-specific siRNAs (#s14100, Thermo Fisher Scientific) and 1.5 μg/106 of the pMax-TSP-1-GFPSpark construct ...
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bioRxiv - Cancer Biology 2024Quote: ... One to four thousand AT2 cells were resuspended in 50 µl complete human alveolar organoid media [Advanced DMEM (Thermo Fisher Scientific, #12491015), 1% GlutaMax (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: SNU1967 cells were transfected with ON-TARGETplus Human siRNA SMARTpool targeting SOX9 (L-021507-00-0005) using Lipofectamine RNAiMAX (Thermo Fisher Scientific). Post-transfection ...
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bioRxiv - Synthetic Biology 2024Quote: Total secreted HSA concentrations of 72 hr yeast HSA expression cultures were quantified via commercially available Albumin Human ELISA Kit (ThermoFisher Scientific #EHALB), following the included protocol ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained with Fixable Viability Dye eFluor 780 and the following monoclonal antibodies: TexasRed-PE conjugated anti-human CD3 (clone 7D6, Invitrogen, cat. MHCD0317); PerCP/Cyanine5.5 conjugated anti-human CD4 (clone OKT4 ...
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bioRxiv - Microbiology 2024Quote: Supernatant collected from human tissues at 48 or 72 hpi after infection with RVFV ZH501 or LAVs (ΔNSs, ΔNSm, MP-12, ΔNSs/ΔNSm) was used to detect total human interferon alpha (IFNα; Invitrogen catalog #BMS216) and human interleukin 29 (IL-29/IFN-λ1 ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... CR3022 antibody (10 μg/mL) and 10 μg/mL of goat anti-human IgG (H+L) secondary antibody Alexa Fluor 647 (Invitrogen #A-21445) was pre-incubated for 30 min at 4 °C ...
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bioRxiv - Biophysics 2024Quote: ... and further subcloned into pAAV2 under the control of the human synapsin promoter and flanked by lox71 and lox66 sites using EcoRI and BamHI restriction sites (FastDigest, Thermo Fisher Scientific). The constructs were soma-targeted using the Kv2.1 targeting domain.
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bioRxiv - Immunology 2024Quote: ... Program T-023 with 150 pmol/106 cells of human TSP-1 and TSP-4-specific siRNAs (#s14108 and #s14100, Thermo Fisher Scientific) and control scrambled siRNAs (#4390846 ...
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bioRxiv - Biophysics 2021Quote: MRC5 cells (human lung fibroblasts, ATCC® Cat# CCL-171™, RRID:CVCL 0440) were maintained in MEM media (Cat# 11095080, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2020Quote: ... h-iECs were dissociated into single cells and sorted into CD31+ and CD31-cells using magnetic beads coated with anti-human CD31 antibodies (DynaBeads, ThermoFisher, Cat No. 11155D). The purified CD31+ h-iECs were then expanded in culture on 10-cm dishes coated with 1% gelatin ...
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bioRxiv - Immunology 2021Quote: Human ACE2-transfected HEK293 cells (prepared in-house) were collected and washed with FACS buffer supplemented with 2% FBS (GIBCO, CN: 10099-148) in 1X PBS ...
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bioRxiv - Immunology 2021Quote: ... at the concentration 1×106 cells per ml for TNF-α assessment in myeloid cells or Dynabeads Human T-Activator CD3/CD28 (ratio 1:2, Thermo Fisher Scientific) for IFN-γ assessment in T-cells in the presence of protein transport inhibitor (BD GolgiStop™ ...
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bioRxiv - Genomics 2020Quote: Optimal establishment of primary human hepatocytes in tissue culture required a substratum of type 1 collagen (24-well plates; ThermoFisher Scientific #A11428-02) and initial plating at 200,000 cells/well in complete Williams’ E medium containing supraphysiological concentrations of insulin ...
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bioRxiv - Genomics 2020Quote: Small-interfering (si)RNA knockdown of LOC157273 was performed on primary human hepatocytes from donor Hu8200 (genotype G/G at rs4841132) plated into collagen-coated 6-well plates (ThermoFisher Scientific #A11428-01). Cells were incubated in complete Williams’ E medium and transfected with siRNA (50 nM ...
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bioRxiv - Bioengineering 2019Quote: ... Jurkat and Human B-lymphoid cell lines were initially tested for mycoplasma contamination and then cultured in RPMI-1640 medium (Life Technologies, Zug, Switzerland) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2020Quote: ... B lymphocytes were obtained by negative cell selection using the MagniSort Human B-cell enrichment kit II (Thermo Fisher Scientific, Carlsbad, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: The cytotoxicity of fluoro-TEMPO and CTEMPO against human T24 urinary bladder epithelial cells was examined utilizing the Pierce™ LDH cytotoxicity assay kit (Life Technologies, Australia) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... and treated with Aß-S8C dimer was subjected to hybridization in duplicates on the GeneChip PrimeView Human Gene Expression Array (Affymetrix, Thermo Fisher Scientific) at the BMFZ (Biomedizinisches Forschungszentrum ...
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bioRxiv - Biochemistry 2019Quote: For mammalian expression a DNA polynucleotide encoding the transmembrane domain (residues 1-295) of GtACR1 optimized for human codon usage was cloned into the pcDNA3.1 vector (Life Technologies, Grand Island, NY) in frame with an EYFP tag ...
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bioRxiv - Cancer Biology 2019Quote: ... RWPE1 (human prostate carcinoma) were cultured in serum-free keratinocyte media containing bovine pituitary extract and epidermal growth factor (GIBCO Invitrogen, Carlsband, CA) at 37°C and 5% CO2 ...
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bioRxiv - Bioengineering 2019Quote: ... was performed on the Rituximab expressing cell pool by staining with an anti-human IgG antibody conjugated to FITC (Invitrogen, Carlsbad, CA, USA) and subsequent single-cell sorting using fluorescence activated cell sorting (FACS ...
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bioRxiv - Cell Biology 2019Quote: Human RBCs (1% in PBS) were double labeled with the lipidic dye R18 and the content marker calcein-AM (Molecular Probes, Life technologies). 20 µl of R18 (1 mg/ml in ethanol ...
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bioRxiv - Cell Biology 2019Quote: ... gondii parasites were maintained by serial passage in confluent human foreskin fibroblast (HFFs, ATCC# SCRC-1041) monolayers in Dulbecco’s Modified Eagle’s Medium (DMEM, Life Technologies-Gibco, Cat# 10569-010), supplemented with 1% (v/v ...
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bioRxiv - Cancer Biology 2019Quote: ... labeled cDNA targets were generated with the Encore® Biotin Module (Nugen) and hybridized to a GeneChip® Human Transcriptome Array 2.0 (Affymetrix, Thermo Fisher) following manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: The human hepatocellar carcinoma (HCC) cell line SNU-398 was cultured in Roswell Park Memorial Institute 1640 medium (RPMI) (Life Technologies, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2021Quote: MOLM-13 cell clones cultured on-chip for 72 h were stained for the hematopoietic marker CD45 using a PE-conjugated primary antibody (mouse anti-human CD45-PE, Invitrogen #12-0451-82). Prior to imaging ...
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bioRxiv - Bioengineering 2020Quote: CD4+ T cells were electroporated prior to stimulation with anti-CD3/CD28 beads (Dynabeads Human T-Activator CD3/CD28, Thermo Fisher Scientific (ThermoFisher), Waltham ...
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bioRxiv - Molecular Biology 2020Quote: ... 150 ng of labelled probe was combined with 5 μg of salmon sperm and 10 μg of human Cot1 DNA (Invitrogen, Cat No 15279011). Two volumes of ethanol were added and the probe mix was collected by centrifugation and dried ...