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Citations for Thermo Fisher :
7701 - 7750 of 7943 citations for Recombinant Human Leptin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... The over-expression construct of human LY6 was created by PCR amplification of cDNA by Phusion® High-Fidelity DNA polymerase (Thermo Fisher Scientific), using the following primers LY-6S-Pac1-S-5’-GGAACGTTAATTAACGCTGAAGTTTGTCTGTGCACTA-3’ ...
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bioRxiv - Molecular Biology 2022Quote: ... H1944 (human lung adenocarcinoma, ATCC, CRL-5907), and H441 (human lung adenocarcinoma, ATCC, HTB-174) cells were maintained in RPMI 1640 medium (Thermo Fisher Scientific, A1049101) with 10% FBS and 1% penicillin-streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: Human embryonic kidney cells (HEK293T, ATCC, Cat #CRL-3216) were cultured in Dulbecco’s modified eagle medium (DMEM, Thermo Fisher Scientific, Invitrogen, Cat#11965092) supplemented with 10 % (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... The iPSCs were cultured on irrMEF with medium for human iPSCs : KnockoutTM-Dulbecco’s modified Eagle’s medium (KO-DMEM: 10829-018, Life Technologies; Thermo Fisher Scientific, MA, USA) supplemented with 20% KnockoutTM-Serum Replacement (KO-SR ...
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bioRxiv - Systems Biology 2021Quote: ... MDA-MB-231 human breast cancer cells were cultured in DMEM/F12 (1:1) media52 (Thermo Fisher Scientific, Life Technologies, Item #11330-032) supplemented with 10% Fetal Bovine Serum (Atlanta Biological ...
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bioRxiv - Bioengineering 2021Quote: ... MDA-MB-231 (ATCC) and MCF7 (ATCC) human mammary cancer cells were both cultured in 1:1 DMEM/F-12 (Thermo Fisher Scientific, USA) media supplemented with 10% fetal bovine serum (Atlanta Biological ...
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bioRxiv - Immunology 2021Quote: ... The pcDNA 3.1+ vectors were then transfected individually into human embryonic kidney (HEK293T) suspension cells using ExpiFectamine 293 transfection reagent (ThermoFisher Scientific, Waltham, MA, USA) following manufacturer’s specifications ...
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bioRxiv - Pathology 2021Quote: ... for 1 week and pepsin (1 mg/ml in 0.1N HCl) was used for antigen retrieval for immunostaining with human COMP (Thermofisher; MA1-20221, 1:100), CHOP (Santa Cruz ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT-PCR was performed using TaqMan gene expression assays (Human-Assay ID Hs00917510_m1; and (Rat-Assay ID Rn01489483_m1; Applied Biosystems, Life Technologies, Carlsbad, CA). Gene expression was quantified by the comparative Ct value ...
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bioRxiv - Cell Biology 2021Quote: The whole genome siRNA screen was conducted as described (35) using HeLa S3 cells and the Dharmacon ON-TARGETplus® SMARTpool® human siRNA library (Thermo Scientific) and strain T3SA+.
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bioRxiv - Cancer Biology 2020Quote: ... human NSCLC cell lines and HARA human lung squamous carcinoma cell line were maintained in RPMI-1640 supplemented with 10% FBS (Gibco BRL, Gaithersburg, MD). Cell transfection was carried out by Lipofectamine 2000TM (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... and after 24h of incubation fresh medium was added and cells were transfected with 100ng of total RNAs from cell cultures or human tissues using Lipofectamine 2000® (11668-019, Thermo Fisher Scientific). As positive controls ...
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bioRxiv - Cell Biology 2021Quote: Human epithelial colon adenocarcinoma, Caco2, cells (ACC 169, DSMZ, Leipzig, Germany) were grown in DMEM/F-12 (Gibco, Thermo Scientific, Waltham, Massachusetts, USA) supplemented with 10 % heat inactivated fetal bovine serum (Biowest ...
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bioRxiv - Cancer Biology 2021Quote: ... and metastasis human colon cancer cell line (SW-620) was cultured in Roswell Park Memorial Institute medium 1640 (RPMI 1640: Gibco, Thermo Fisher, USA). All media were supplemented with 10% fetal bovine serum (FBS ...
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CRISPR screens for lipid regulators reveal a role for ER-bound SNX13 in lysosomal cholesterol exportbioRxiv - Cell Biology 2021Quote: ... cells were transfected with siRNAs targeting human SNX13 (5’- CAGAAAGGCUCAACAGAAAUU-3’) or SNX14 (5’-GGAUGAAAGUAUUGACAAAUU-3’) using Lipofectamine RNAiMax (Invitrogen, Cat#13778-075) according to the manufacturer ...
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bioRxiv - Neuroscience 2022Quote: ... using a commercial human fetal brain cDNA library containing cDNAs fused to the gal4 activation domain of pEXP-AD502 (ProQuest™, ThermoFisher Scientific, USA), as prey ...
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bioRxiv - Cancer Biology 2022Quote: ... A CRISPR based kinome-wide screening was performed using a lentiviral sgRNA library (LentiArray™ Human Kinase CRISPR Library, Thermo Fisher Scientific, M3775) that knocks out 840 kinase and kinase related genes individually with total number of 3214 sgRNA constructs ...
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bioRxiv - Immunology 2021Quote: ... Fresh human peripheral blood mononuclear cells were used as effector cells after red blood cell lysis with ACK lysing buffer (ThermoFisher Scientific, Waltham, MA). After 1 h incubation at 37°C ...
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bioRxiv - Neuroscience 2021Quote: Post-selection iPSCs with stably-integrated human NGN2 under a tetracycline-inducible promoter were dissociated into single cells using Accutase (Thermo Scientific Cat. #A1110501) and plated on a Matrigel (1:100 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stained for 30 min on ice using AlexaFluor700-conjugated anti-Human CD54/ICAM-1 (clone 1H4, Life Technologies, #MA528553, 1/75), PE-Cy7-conjugated anti-Human CD106/VCAM-1 (clone STA ...
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bioRxiv - Neuroscience 2021Quote: Lund human mesencephalic (LUHMES) neuronal precursors were grown in T75 flasks in proliferation medium (DMEM/F12 GlutaMAX™ supplement medium (Thermo Fisher Scientific), N2 supplement (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... and the N-terminal peptidase domain of human ACE2 were expressed using the Bac-to-Bac Baculovirus Expression System (Invitrogen, Carlsbad, CA, USA) and purified by Strep-Tactin Sepharose (IBA Lifesciences ...
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bioRxiv - Cancer Biology 2022Quote: ... Panc1 and MiaPaCa2 with RBFOX2 knockdown and non-targeting control were generated using human Affymetrix Clariom D Arrays (Life Technologies, Cat. no. 902922) with the standard input kit run on the Applied Biosystems GeneChip 3000 instrument ...
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bioRxiv - Cell Biology 2022Quote: ... MCF10A human breast epithelial cells (CRL-10317, ATCC, Manassas, VI, USA) were maintained in DMEM/F12 medium (11039-021, Invitrogen, Carlsbad, CA, USA) supplemented with 20 ng.ml-1 epidermal growth factor (AF-100-122 ...
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bioRxiv - Pathology 2019Quote: Mouse podocytes generated from wild-type and INF2 KO C57BL/6 mice (Supplementary Figure 3) and human podocytes (43) were maintained in RPMI 1640 (ThermoFisher Scientific, Waltham MA) supplemented with 10% fetal bovine serum (ThermoFisher) ...
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bioRxiv - Genomics 2019Quote: ... All primer pairs were tested alone and in multiplexed PCR reactions using 10 ng of TaqMan® Control Human Genomic DNA (Thermo Fisher Scientific) in 10 μl reaction volumes ...
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bioRxiv - Systems Biology 2020Quote: ... The amount of VEGF contained in the thawed medium was measured using the protocols and reagents from the VEGF Human ELISA Kit (BMS277-2, Thermo Fisher Scientific, USA), and the amounts of PEDF were measured using a Human ELISA Kit (RD191114200R ...
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bioRxiv - Immunology 2021Quote: ... 32,33 were maintained by serial 2-day passaging in human foreskin fibroblast (HFF-1, ATCC® SCRC-1041™, American Tissue Culture Collection) monolayers cultured in DMEM (Thermofisher scientific) with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... HeLa cells in 35-mm dishes were transfected with 100 pmol of each siRNA in the Stealth siRNA library targeting 154 human nuclear proteins (Invitrogen–Thermo Fisher Scientific) using Lipofectamine RNAiMax (Invitrogen–Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2019Quote: ... the VEGF-A amount in the conditioned medium of day 14 was measured using a VEGF-A Human ELISA Kit (Thermo Fisher Scientific, USA). The assay was performed according to the manufacturer’s protocol and absorbance was read at 450nm ...
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bioRxiv - Genetics 2020Quote: ... T cells were activated through the T cell receptor subunit CD3 and the co-stimulator CD28 by the addition of Human T-Activator CD3/CD28 Dynabeads at a 1:2 bead:cell ratio (Life Technologies, cat. no. 111.31D) for 72 hours.
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bioRxiv - Cell Biology 2020Quote: ... human milk cells were isolated by diluting milk samples in an equal volume of sterile phosphate-buffered saline (PBS) (ThermoFisher Scientific, Waltham, U.S.) and centrifuged at 870 g for 20 minutes at 20°C in a Rotanta 460R centrifuge (Hettich ...
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bioRxiv - Biochemistry 2020Quote: The cDNA encoding a fusion protein consisting of GST followed by a 3C cleavage site and the human SMAD2 MH2 domain (residues 241–465) was inserted into the MCSI of the pFastBac™ Dual vector (Thermo Fisher Scientific) in the Sal1 and Spe1 restriction sites ...
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bioRxiv - Cancer Biology 2021Quote: Full-length human SETD7 cDNA sequences were amplified from a human mRNA pool generated by RT-PCR using SuperScript II RNase H Reverse Transcriptase (Life Technologies,CA, USA). The SETD7 cDNA was cloned into the EcoRI/BamHI site of a pCDH-CMV-MCS-EF1α -Puro vector (System Biosciences ...
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bioRxiv - Genetics 2021Quote: ... 250 000 cells in 2 ml of medium in a six-well plate were transfected using 12.5 pmol of Silencer Select siRNA against human ADCY9 (Ambion cat # 4390826 ID 1039), Silencer Select siRNA against CETP (Ambion cat 4392420 ID 2933 ...
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bioRxiv - Microbiology 2020Quote: ... Briefly, parasites were maintained in 2% human O+ erythrocytes (Interstate Blood Bank, Memphis, TN) in RPMI-1640 medium (Life Technologies, Grand Island, NY) supplemented with 0.5% Albumax II (Life Technologies) ...
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bioRxiv - Immunology 2020Quote: ... 4 were used to measure the message levels of these human or mouse genes in cells or lung tissue (Life Technologies, Carlsbad, CA).
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bioRxiv - Immunology 2021Quote: ... The human embryonic kidney 293T/17 cells (ATCC, CRL-11268) were maintained in maintained in Dulbecco modified Eagle medium (Gibco Life Technologies, Gaithersburg, Md.) containing 10% fetal calf serum (FCS) ...
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bioRxiv - Microbiology 2021Quote: ... Cell suspensions prepared by enzyme-free CDS were kept in PBS-5% FCS and incubated on ice for 30 min with rabbit polyclonal anti-human HVEM (R140; 1:500) (Terry-Allison et al., 1998) followed by visualizing HVEM with anti-rabbit AF488 (Life Technologies; 1:200). The size of the epidermal samples mostly allowed only the analysis of nectin-1 expression ...
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bioRxiv - Neuroscience 2022Quote: Cell culture supernatants were analyzed for secreted proteins using Immune Monitoring 65-Plex Human ProcartaPlex™ Panel for MAGPIX (Thermofisher Scientific, Carlsbad, CA) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Cell culture supernatants were analyzed for secreted proteins using Immune Monitoring 65-Plex Human ProcartaPlex™ Panel for MAGPIX (Thermofisher Scientific, Carlsbad, CA) as per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... HTB-14) human glioblastoma cells were cultured in Dulbecco’s modified Eagle medium/nutrient mixture F-12 (DMEM/F-12, ThermoFisher Scientific cat. no. 11330057) supplemented with 10% fetal bovine serum (FBS premium grade ...
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bioRxiv - Bioengineering 2022Quote: ... Primary human MSCs (Cyagen Biosciences, Suzhou, China) from bone marrow were cultured in a minimum essential medium alpha (MEM-α, Gibco, Carlsbad, CA) medium with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: Paraffin-embedded human lung tissue of control and IPF donors was cut with a Microm HM355S microtome (Thermo Fisher Scientific, Waltham, MA, USA) into serial sections of 3 μm ...
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bioRxiv - Immunology 2022Quote: ... Electroporated T cells recovered overnight in IL7 and Il15 medium were then activated by anti-human CD3/CD28 beads (Thermo Scientific, Cat.11131D) at 1:1 ratio for two days ...
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bioRxiv - Microbiology 2023Quote: ... [72] at 4 °C overnight followed by staining with 2 µg/ml of secondary AlexaFluor647-conjugated Donkey anti-human IgG antibody (ThermoFisher Scientific, OR, USA) at room temperature for 45 min ...
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bioRxiv - Neuroscience 2022Quote: ... HLA-DR Antibody (Monoclonal Rabbit Anti-Human, Citrate Buffer HIER, dilution 1:50 Clone: LN-3, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), TMEM119 (Rabbit Anti-Human ...
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bioRxiv - Physiology 2022Quote: Human TRPM6 was either transfected in a bicistronic vector pCINeo/IRES-GFP plasmid or in a pcDNA pDEST53 plasmid (ThermoFisher Scientific, Waltham, MA) (26 ...
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bioRxiv - Molecular Biology 2022Quote: Human adult epidermal keratinocytes were cultured in EpiLife medium supplemented with 10% Human Keratinocyte Growth Supplement (HKGS) and 1% Penicillin and Streptomycin at 37℃ in 5% CO2 (all from ThermoFisher Scientific, Waltham, MA). Calcium concentration was increased to 1.5 mM in the medium to induce cell differentiation ...
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bioRxiv - Bioengineering 2022Quote: Epidermal growth factor receptor (EGFR) protein expression levels on the surface of Gli36-derived EVs were quantified using an EGFR Human ELISA kit (ThermoFisher Scientific, Waltham, MA). EVs were spiked in healthy donor serum at different concentrations ranging from 0 to 1.0E11 particles/mL while maintaining the serum-derived EV concentration at 1.0E9 particles/mL ...