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Citations for Thermo Fisher :
7551 - 7600 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... Cells were loaded with 10 μmol/L Fluo-3-AM (Invitrogen; 10 min loading and 30 min de-esterification ...
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bioRxiv - Cell Biology 2022Quote: ... Blocking was performed with 3% bovine serum albumin (Thermo Scientific, # 26140079) and 2% goat serum diluted in PBS containing 0.25% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... RNA samples were treated with 3 U of TURBO DNase (Ambion) for 15 min at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... on a QuantStudio 3 real-time PCR system (Thermo Fisher Scientific). Fold change in gene expression compared to endogenous controls was calculated using the ddCT method ...
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bioRxiv - Cell Biology 2022Quote: ... DLP1-sense strand: 5’-UCCGUGAUGAGUAUGCUUUdTdT-3’ 31 (Ambion, Austin, TX, USA).
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bioRxiv - Biochemistry 2022Quote: ... 3 µL 10X sample reducing agent (Life Technologies, Carlsbad, CA, USA) and heat-denaturation for 5 min at 95 °C ...
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bioRxiv - Bioengineering 2022Quote: ... and 0.05 M of Fe(NO3)3 (Thermo Fisher Scientific Corp.) until they were approximately the same shade of blue ...
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bioRxiv - Molecular Biology 2022Quote: ... and either TO-PRO-3-Iodide (Thermo Fisher Scientific, Waltham, MA) or DAPI (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... BxPC-3 and PSN-1 were cultured in RPMI1640 (Thermo Fisher), supplemented with 10% FCS and 1% P/S ...
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bioRxiv - Neuroscience 2022Quote: ... The RTqPCR were perfomed using a QuantStudio™ 3 System (ThermoFisher) with SYBR Green enzyme mix (ThermoFisher PowerUp SYBR Green Master Mix) ...
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bioRxiv - Genomics 2022Quote: ... The GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) Taqman probe (Thermo Fisher Scientific) was used as an endogenous control.
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bioRxiv - Microbiology 2022Quote: Quantitative RT-PCR (QuantStudio 3, Applied Biosystems, Foster City, CA, USA) was performed with one-step Prime script III RT-qPCR mix (Takara ...
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bioRxiv - Developmental Biology 2022Quote: ... After treatment with 3 U DNase I (AmpGrade; Thermo Fisher Scientific), sgRNAs were purified using High Pure PCR Cleanup Micro Kit (Roche) ...
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bioRxiv - Cell Biology 2022Quote: ... spreads were blocked in 3% BSA (w/v) (Fisher Scientific, 11483823) in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... IBC-3 and SUM190 in Ham’s F-12 media (GIBCO, #31765092) with 1 μg/ml hydrocortisone and 5 μg/ml Insulin ...
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bioRxiv - Microbiology 2022Quote: ... 1 nM TO-PRO™-3 Iodide (642/661 nm, Thermofisher) and 8 ng/ml calcofluor-white (CW ...
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bioRxiv - Microbiology 2022Quote: ... 1 nM TO-PRO™-3 Iodide (642/661 nm, Thermofisher) and 8 ng/ml calcofluor-white (CW ...
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bioRxiv - Microbiology 2022Quote: ... Quantitative RT-PCR (QuantStudio 3, Applied Biosystems, Foster City, CA, USA) was performed using one-step Prime script III RT-qPCR mix (RR600A ...
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bioRxiv - Cell Biology 2022Quote: ... Approximately 3 million cells were washed twice with Opti-MEM (Gibco) and then resuspended in 90 µL Opti-MEM with 10 µg of DNA in an electroporation cuvette with a 2 mm gap ...
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bioRxiv - Cancer Biology 2022Quote: ... digested in DMEM media containing 3 mg/ml Dispase II (Gibco) and 1mg/ml Collagenase IV (C5138 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were counted using the Countess 3 Automated Cell Counter (ThermoFisher).
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bioRxiv - Developmental Biology 2021Quote: ... Pellet was resuspended with warm 3 ml TrypLE express (Gibco, 12604021), incubated for 30 minutes at 37°C in a shaker at 180 rpm ...
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bioRxiv - Molecular Biology 2020Quote: ... which involved 3 rinses with phosphate-buffered saline (PBS; Gibco, NY), division of the tissue into tiny pieces ...
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bioRxiv - Neuroscience 2021Quote: ... fluorescein and biotin labelled 3 kDa soluble lysine fixable (D7156, Invitrogen) and ovalbumin Alexa Fluor® 594 conjugate 45 kDa (O34783 ...
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bioRxiv - Immunology 2020Quote: ... 3 µg of total RNA were treated with DNase I (Invitrogen, DNase1 amplification grade Cat # 18068-015 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... supplemented with 3% foetal bovine serum (heat-inactivated; ThermoFisher Scientific, Poland) and antibiotics ...
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bioRxiv - Biochemistry 2021Quote: ... the cells were washed 3 times with 2 mL DPBS (Gibco), scraped and lysed in 4% SDC buffer (4% sodium deoxycholate ...
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bioRxiv - Cancer Biology 2020Quote: ... caspase-3 and caspase-7 green apoptosis-assay reagent (Life Technologies) was added to the culture medium following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... and were passaged every 3 days using Versene (EDTA-based, Gibco) when 80% confluent ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were cultured in Stage 3 media containing IMDM 47.5% (Gibco), 47.5% F12 ...
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bioRxiv - Microbiology 2022Quote: ... OD600 of supernatant was determined (BioMate 3 Thermo Spectronic, Fisher Scientific).
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bioRxiv - Genetics 2022Quote: ... Quantitative PCR was performed on QuantStudio 3 (Applied Biosystems, MA, USA) following the parameters recommended by the manufacturer ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were collected into FAD media (DMEM/F12 3:1 (Gibco) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... on a QuantStudio 3 real-time PCR system (Thermo Fisher Scientific). Fold change in gene expression compared to endogenous controls was calculated using the ddCT method ...
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bioRxiv - Immunology 2020Quote: ... 3:100 of anti-CD8 PE-Texas red (3B5, Thermo Fisher), 1:50 of anti-CD26 FITC (BA5b ...
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bioRxiv - Molecular Biology 2020Quote: ... after 3 washes cells were incubated with FxCycleTM Violet Stain (ThermoFisher). The positive/negative gates for pH3S10 were gated on a negative control ...
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bioRxiv - Physiology 2021Quote: ... Cells were counterstained with nuclear TO-PRO-3 (Life Technologies, #T3605) or DAPI (Invitrogen ...
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bioRxiv - Bioengineering 2020Quote: ... N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide HCl (50-848-678, Fisher Scientific), butyric acid (B103500-100ML ...
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bioRxiv - Microbiology 2021Quote: ... Capped RNA (3 μg) was mixed with Lipofectamine™ 3000 (Invitrogen) transfection reagents ...
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bioRxiv - Physiology 2020Quote: ... and 3 mg/mL bovine serum albumin (BSA, Fisher Scientific, UK) for 1 h on ice ...
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bioRxiv - Immunology 2020Quote: ... anti-CD3-PerCP/Cy5.5 (clone OKT-3, Thermo Fisher Scientific, USA), anti-mouse TCRβ PacificBlue (clone H57-597 ...
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bioRxiv - Immunology 2020Quote: ... IL-1β and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (ThermoFisher Scientific, USA). The relative expression of the above factors was determined by the 2-△△Ct method in which samples were normalized to GAPDH and expressed as fold change over uninfected controls.
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bioRxiv - Genomics 2021Quote: ... for RPE1] in 1:3 ratio in Opti-MEM media (Gibco), incubated for 15 min at RT ...
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bioRxiv - Microbiology 2020Quote: ... the reaction product resorufin was read by fluorometer (QUBIT 3, Invitrogen) at Ex/Em of ...
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bioRxiv - Cell Biology 2020Quote: ... and Taqman probe: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH, Hs02758991_g1) (Applied Biosystems) were used to perform real-time PCR reactions over 40 cycles according to manufacturer’s instructions on the StepOnePlus system ...
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bioRxiv - Physiology 2020Quote: ... and a QuantStudio 3 Flex Real-Time PCR System (Applied Biosystems). Relative expression was determined using the ΔΔCt method and normalizing to PPIA (cyclophilin A) ...
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bioRxiv - Biochemistry 2021Quote: ... In a NuPAGE 3-8 % Tris-Acetate gel (NOVEX Life Technologies), 15 μg protein was loaded with 5 x loading buffer (0.05 % Bromophenol Blue ...
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bioRxiv - Cancer Biology 2021Quote: ... 72°C - 3 min) then cloned into Topo PCR 2.1 (Invitrogen) and sequenced ...
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bioRxiv - Zoology 2021Quote: ... and CellEvent Caspase-3/7 Green ReadyProbes Reagent (ThermoFisher Scientific, R37111) (CellEvent ...
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bioRxiv - Genomics 2021Quote: ... followed by 3 mL of methanol (Fisher Scientific; Cat #A454-4), and 600 µL of 3% HCl (Sigma-Aldrich ...