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Citations for Thermo Fisher :
7551 - 7600 of 10000+ citations for Recombinant Human IL18 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... Supernatant (cleared lysates) were used to determine total protein amount using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... Supernatant (cleared lysates) were used to determine total protein amount using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... and the protein concentration determined for normalization purposes using a Pierce BCA protein assay kit (Thermo Scientific, Waltham, MA, USA). The remainder of the sample was heated to 95°C for 10 min ...
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bioRxiv - Biochemistry 2021Quote: ... The remaining soluble protein fraction was collected and quantified for protein concentration via bicinchoninic acid (BCA) assay (Pierce Thermo Fisher). The soluble protein concentration was then used as an indication of lysis efficiency/cell mass to determine the amount of 8 M urea (dissolved in PBS ...
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bioRxiv - Genomics 2021Quote: ... The chromatin fragments bound to antibodies (after incubation overnight) were captured using 40 μl of protein-A or protein-G Dynabeads (Invitrogen; pre-washed with 0.1% BSA [Fraction V ...
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bioRxiv - Biophysics 2021Quote: ... The SNAP-tag allows for covalent binding of O6-benzylguanine (BG) to the protein.[63] Protein expression was performed in Escherichia coli BL21(DE3) pRARE (Invitrogen) under T7 promoter with 1 mM isopropyl-β-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Cell Biology 2020Quote: ... Equal loading was ensured by conducting protein concentration measurements with the Pierce BCA Protein Assay Kit (Thermo Scientific, cat# 23225). After SDS-PAGE and western blot transfer ...
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bioRxiv - Microbiology 2021Quote: ... cytoplasmic proteins and the total cell lysate proteins were denatured and boiled with 4× LDS sample buffer (Cat. #NP0007, Invitrogen) and subjected to immunoblotting analysis with equal protein loading of extracts (~20 µg/lane) ...
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bioRxiv - Microbiology 2020Quote: ... nuclear soluble and chromatin-bound protein fractions were extracted from cells using the Subcellular Protein Fractionation Kit for Cultured Cells kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... total cell protein was extracted and subjected to co-immunopreciptation (Co-IP) with Dynabeads-Protein G (Thermo Fisher Scientific, USA) followed by the protocol described previously (12).
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bioRxiv - Immunology 2020Quote: ... and the supernatant was collected to determine the protein concentration using the PierceTM BCA protein Assay kit (Thermo Fisher Scientific). 1x sample SDS buffer was added to the supernatant for electrophoresis ...
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bioRxiv - Neuroscience 2020Quote: ... Supernatants containing the soluble proteins were subjected to SDS-PAGE on NuPAGE™ 4–12% Bis-Tris Protein Gels (Invitrogen) in the Mini Gel Tank and Blot Module Set (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2021Quote: ... the supernatant was collected and the protein concentration was measured with Piece™ BCA protein kit (23227, Thermo Fisher Scientific) according to the manufacturer’s instruction ...
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The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: ... Soluble chromatin supernatant equivalent to 6 μg per IP was pre-cleared using 20 μl of a 1:1 mix of protein A and protein G Dynabeads (Invitrogen). Overnight IP was performed using 1 μg of anti-H3K4me3 (Diagenode ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were lysed with 15 μl RIPA buffer and protein concentration was quantified using BCA Protein Assay Kit (Thermo Scientific). OCR measurements were normalized to the protein concentration in each well.
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bioRxiv - Bioengineering 2022Quote: ... using a ratio of 1 µg trypsin to 50 µg sample protein as quantified using a Pierce BCA Protein Assay Kit (ThermoFisher). Samples were digested overnight at room temperature using a rotor for mixing and then placed in a speed vacuum to reduce sample volume ...
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bioRxiv - Molecular Biology 2022Quote: Total protein was extracted from homogenized whole kidney lysates and RPTECs using mammalian protein extraction reagent (MPER, ThermoFisher Scientific, 78501) and Ambion PARIS kit ...
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bioRxiv - Biochemistry 2022Quote: ... The molecular weight of the purified protein was estimated by SDS-PAGE with Protein Plus prestained ladder (Thermo Fisher Scientific). UV–visible spectra of purified SynLaspo were recorded using a Varian Cary 50 BIO spectrophotometer.
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Proteins were then quantified using a Bradford Protein assay according to manufacturer’s instructions (Pierce Coomassie Plus Bradford Protein Assay Reagent, Thermofisher Scientific). Samples and albumin standards were run in triplicate on a 96-well optical plate and quantified on a BioTek Synergy H1 96-well plate reader (Winooski ...
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bioRxiv - Biochemistry 2022Quote: ... reaction mixtures containing approximately 5 μM protein in DSF buffer (50 mM Na2HPO4, 150 mM NaCl, pH 7.4) and 1x Protein Thermal Shift Dye (Thermo Fisher) were heated from 20 °C to 99 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... the protein-RNA-beads complexes were washed and proteins were eluted in Elution Buffer (Pierce/Thermo Fisher Scientific, Stockholm, Sweden), boiled at 95°C for 5 min and subjected to SDS-PAGE ...
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bioRxiv - Cell Biology 2022Quote: ... The antibodies and any bound proteins were purified by incubating the lysates with protein A/G magnetic beads (Thermo Scientific), washing the beads three times with lysis buffer and eluting bound proteins with 2% SDS in PBS.
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bioRxiv - Cancer Biology 2022Quote: ... The cleared supernatant was kept on ice and protein was quantified using the Qubit Protein Assay kit (Thermo Scientific, Q33212) and measured on the Qubit 2.0 Fluormeter (Thermo Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... The total protein concentration of each fraction was determined by Bradford using a commercial Coomassie Protein assay reagent (Thermo Fisher).
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bioRxiv - Cancer Biology 2022Quote: ... Clarified lysates were incubated with 1 ug of YB-1 antibody and antibody/protein/RNA complexes were isolated by using 20 μl packed volume protein A sepharose beads (Invitrogen). Beads were washed with polysome extraction buffer and RNA isolated using the PeqGold RNA isolation kit ...
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bioRxiv - Cell Biology 2022Quote: ... the worms were removed from the wells and BCA protein determination (Thermo Scientific™ Pierce™ BCA Protein Assay Kit) was performed and used for normalization ...
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bioRxiv - Cell Biology 2022Quote: ... The total protein content of the samples was estimated using Pierce BCA protein assay kit from Thermo Scientific (Rockford, USA) and 30 μg of total protein content was loaded to a precast 4 to 20% gradient 1-mm thick polyacrylamide mini-gels from Anamed Elektrophorese (Rodau ...
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bioRxiv - Cell Biology 2022Quote: ... and interacting proteins were co-immunoprecipitated with 5 µg of anti-gag antibody per mg of total proteins using Dynabeads protein G (Invitrogen). Proteins were solubilized in Laemmli buffer and separated by SDS–PAGE ...
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bioRxiv - Cell Biology 2022Quote: ... and normalized to the total protein level in the same sample using the BCA Protein Assay Kit (23227, Thermo Scientific). OD values for ELISA and BCA were measured on an InfiniteM200 Pro TECAN plate reader at 450 and 562 nm respectively ...
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bioRxiv - Immunology 2022Quote: ... The membrane proteins of KCs or ECs were enriched using the Mem-PER Plus Membrane Protein Extraction Kit (Thermo Scientific) with Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... lysate-containing supernatants were collected and protein quantities were determined via BCA (Pierce™ BCA Protein Assay Kit, Thermo Scientific). Proteins were denatured by addition of 2X Laemmeli buffer/10% β-mercaptoethanol (Bio-Rad/Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... in ng per mg of protein present in the homogenate determined with BCA Protein Assay Kit (Thermo Scientific, Illkirch, France).
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bioRxiv - Genetics 2022Quote: ... 13000rpm,10 min) to collect protein and the concentration of protein was determined with the BCA assay (Thermo Fisher Scientific). Western blot was performed following the manufacture’s instruction of the corresponding antibodies (Table S19) ...
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bioRxiv - Microbiology 2022Quote: ... and a small aliquot (30 μL) was removed for protein quantification (Pierce BCA Protein Assay Kit; Thermo Fisher, Waltham, Massachusetts). For lipid quantification ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting supernatant was used to quantify total protein using the Pierce BCA protein assay kit (Thermo Scientific, cat# 23225) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations of EL fractions and TL were determined using the Bicinchoninic acid assay (BCA Protein Assay Kit, Thermo Scientific). Bovine serum albumin was used as a protein standard ...
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bioRxiv - Microbiology 2023Quote: ... The protein concentration of the cell lysate was measured by BCA assay (Pierce BCA Protein Assay Kit, Thermo Scientific, USA). The cell pellet was resuspended in 40 μl ddH2O and mixed carefully ...
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bioRxiv - Physiology 2023Quote: ... Proteins were separated by electrophoresis in NuPAGE™ 4 to 12% Bis-Tris Mini Protein Gels (Thermo Fisher Scientific, USA), and then transferred to Immobilon®-FL PVDF Membrane (Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... The protein concentration was calculated by measuring the concentrated proteins with a BCA absorbance assay according to the manufacturer’s instructions (Pierce™ BCA Protein Assay Kit #23225, ThermoFisher).
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bioRxiv - Molecular Biology 2022Quote: ... Protein concentration was measured by BCA according to the manufacturer’s protocol (Thermo Scientific™ Pierce™ BCA Protein Assay Kit) and 4X LDS sample buffer was added ...
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bioRxiv - Neuroscience 2022Quote: ... 20 µL of each sample were withdrawn for total protein concentration determination (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific). Then ...
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bioRxiv - Neuroscience 2022Quote: Protein concentrations of each sample were determined using the bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific, Waltham, MA) which used bovine serum albumin standards to create a best fit linear regression line based on protein concentrations using the 562nm absorbance mode of a microplate reader and its Gen5 Data Analysis program (Biotek ...
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bioRxiv - Plant Biology 2022Quote: ... The total protein concentration for each sample was measured using the Pierce 660 nm Protein Assay kit (Thermo Scientific™). At this point two different experiments were performed ...
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bioRxiv - Molecular Biology 2022Quote: ... The total protein content in the seed preparation was measured by the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). 2% (w/w ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein concentration was measured by BCA according to the manufacturer’s protocol (Thermo Scientific™ Pierce™ BCA Protein Assay Kit) and 4X LDS sample buffer was added ...
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bioRxiv - Molecular Biology 2022Quote: ... we performed RNA-Protein pull-down assay by using Pierce™ Magnetic RNA-Protein Pull-Down Kit (Thermo Fisher Scientific). The biotin labeled RP24-315D19.10 and AS-RP24-315D19.10 probe was designed and synthesized by GenePharma company ...
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... and the resulting supernatants were collected and assayed for protein concentration with a BCA Protein Assay Kit (Thermo Fisher, 23225). Samples containing equal amounts of protein were denatured by boiling for 10 min with Laemmli sample buffer and then fractionated by SDS-polyacrylamide gel electrophoresis ...
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bioRxiv - Biochemistry 2024Quote: ... The protein concentration was determined in a 96-well format using the Pierce BCA protein assay kit (Thermo Fisher Scientific).
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Total protein concentrations in CA1 and amygdala homogenates were quantified using Pierce BCA Protein Assay (Thermo Scientific Cat. No. 23225).
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Total protein concentration of the cleared lysate was assessed with the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). IL-1β was quantified from hippocampi tissue lysates with V-PLEX Mouse IL-1β Kit (Meso Scale Discovery ...