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Citations for Thermo Fisher :
7451 - 7500 of 10000+ citations for Recombinant Human IL18 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... gRNA/Cas9 complexes were formed by incubating 250 ng of the gRNA with 1 µg of purified Cas9 protein (TrueCut Cas9 Protein v2, Invitrogen) in OptiMEM (Invitrogen) ...
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bioRxiv - Microbiology 2019Quote: ... The lysate/antibody mixture was then incubated with either Protein A (for Flag-Riplet experiments) or Protein G Dynabeads (Invitrogen) for 2 hours ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The amount of protein in each sample was determined using a BCA protein assay Kit (Thermo Scientific, Waltham, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were then clarified by spinning at 14,000 rpm at 4°C and protein concentration quantified by BCA Protein Assay (23227, Thermo Scientific). Forty micrograms of proteins were denatured in boiling SDS sample buffer and resolved on 4%-20% gradient gels (5671093 ...
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bioRxiv - Microbiology 2019Quote: ... The samples were heated at 95°C for 10 minutes and centrifuged at 4°C for 10 minutes to remove potential protein aggregates before proteins were separated in gradient polyacrylamide gels (4-12%) (Invitrogen). Proteins were transferred onto nitrocellulose membranes (Whatman ...
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The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... Samples were pre-cleared with Dynabeads Protein A and Dynabeads Protein G (Catalog #10001D and #Catalog #10003D, Thermo Fisher Scientific) for 1hr at 4 °C first and then incubated with rabbit anti-PntP1 antibody (a gift of J ...
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bioRxiv - Immunology 2021Quote: Total protein concentrations in BAL fluid were detected by Pierce BCA Protein Assay Kit according to the manufacturer’s instructions (Thermo Scientific).
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bioRxiv - Biochemistry 2020Quote: ... Protein concentration was measured using Pierce BCA Protein Assay kit according to manufacturer’s instructions (Thermo Fisher Scientific, Waltham, MA, USA). 20 µg of total protein/lane were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Biochemistry 2021Quote: Protein concentration of cell lysates was determined using the PierceTM BCA protein assay kit according to manufacturer’s instructions (ThermoFisher Scientific). Samples were adjusted in 5xLDS sample buffer containing 50 mM DTT ...
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bioRxiv - Biochemistry 2020Quote: Stability of VH1 proteins was determined with a Protein Thermal Shift Dye kit (Life Technologies Corporation, Grand Island, NY, USA) following the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2020Quote: ... The concentration of proteins was measured by the Pierce microBCA Protein Assay according to the manufacturer’s instruction (Thermo Fisher Scientific). Equal amounts of protein for each sample were precipitated with six volumes of pre-chilled (−20 °C ...
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bioRxiv - Biochemistry 2020Quote: Protein biosynthesis assays were carried out using the click-it plus O-propargyl-puromycin (OPP) protein synthesis assay (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations of membrane fractions in collected supernatant samples were measured by BCA protein assay kit (Thermo Fisher Scientific 23225).
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bioRxiv - Genomics 2021Quote: ... 50 µg of proteins from worm lysate and 10 µg of proteins from secretome were resolved on a 12% SDS-PAGE with a BenchMark protein ladder (Invitrogen) and a negative control (SDS-PAGE loading buffer and water) ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein concentration of the supernatants was determined using Pierce 660 nm Protein Assay Reagent Kit (22660, Thermo Fisher Scientific) supplemented with Ionic Detergent Compatibility Reagent (22663 ...
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bioRxiv - Cancer Biology 2020Quote: ... Supernatant of cell lysates was assayed for total protein content using the BCA protein assay (Thermo Fisher Scientific, Rockford, IL), and 70 μg of total protein per sample was separated in a 7.5% polyacrylamide gel (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: ... Labeled proteins were recovered from equivalent amounts of protein by batch affinity chromatography with streptavidin-coupled M280 Dynabeads (Thermo Fisher), 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... the protein concentration of each fraction was determined using a bicinchoninic acid assay (BCA Protein Assay Kit by Thermo Scientific). Samples were stored at −80 °C until further use.
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bioRxiv - Cell Biology 2021Quote: ... 5–12 µl of protein lysate was loaded into pre-cast protein gels (4–12 % Bolt Bis Tris Plus, ThermoFisher) together with 10 µl of the molecular marker (PageRuler prestained ...
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bioRxiv - Systems Biology 2020Quote: ... 20 μg of protein from each sample was separated on NuPAGE 4-12% Bis-Tris protein gels (Thermo Fisher Scientific) and transferred to nitrocellulose membranes (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentration of the elution fraction was determined with the Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA) and the elution fraction with the highest protein concentration was chosen for further use.
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bioRxiv - Neuroscience 2020Quote: Approximately 10μg of each protein extract was separated on a precast gradient gel (NuPAGE 4-12% Bis-Tris Protein Gels, NP0336BOX, Thermofisher) and transferred to PVDF membrane (GE10600022 ...
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bioRxiv - Microbiology 2021Quote: Protein content of samples for SDS-PAGE and immunoblotting was analyzed using a Pierce BCA Protein Assay (Thermo Scientific, Germany) with a standard curve from 0 to 2000 µg mL−1 bovine serum albumin following the manufacturer’s recommendations ...
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bioRxiv - Physiology 2021Quote: ... Supernatant (cleared lysates) were used to determine total protein amount using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... protein was extracted from supernatants and pellets were resuspended directly in protein loading buffer (2x NuPAGE LDS sample buffer (Invitrogen), 715 mM 2-mercaptoethanol) ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein content of all extracts was determined using the Pierce BCA Protein Assay Kit (Cat no. 23225, ThermoFisher Scientific) using bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2021Quote: ... The pellet was re-suspended in 8M urea/50mM Tris pH8 and protein concentration was determinate with Qubit Protein Assay (Invitrogen).
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bioRxiv - Cancer Biology 2021Quote: ... phosphatase inhibitors) and proteins concentrations were measured using a Pierce BCA protein Assay Kit (Thermo Fisher Scientific, Ref: 23225/23227). 10 μg of proteins were then separated on a 4-15% Mini-PROTEAN TGX Stain-Free Gel (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein samples (50µg total protein) were resolved by SDS-PAGE using 4-12% Bis-Tris gel (Nupage, Invitrogen, Carlsbad, CA) and transferred to a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... the protein was centrifuged at 10,000×g for 10 min and quantified by BCA Protein Assay Kit (Thermo Fisher Scientific). The same quantity (about 15 μg ...
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bioRxiv - Cell Biology 2020Quote: ... per 1 mg of total protein were added along with 30 μL washed protein A/G UltraLink Resin (53133, ThermoFisher) and rotated overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... Total protein amount was measured from 10 µL of the sample using the Pierce BCA protein assay kit (Thermo Fisher). The levels of radioactive methionine were measured by scintillation counting with 10-80 µL of the resuspended protein ...
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bioRxiv - Cell Biology 2022Quote: ... DNA- protein complexes were pulled down with addition of 10 μl of pre-washed Protein G Magnetic Beads (Invitrogen, 10003D) and incubation for 1-2 hours at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was clarified by centrifugation and the total protein concentration was measured with the BCA Protein Assay (Thermo Fisher Scientific) to enable input standardization ...
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bioRxiv - Cell Biology 2022Quote: ... was used to lyse cell pellets and protein concentration determined using the Micro BCA Protein Assay kit (ThermoFisher Scientific, 232350). 10 μg protein ...
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bioRxiv - Neuroscience 2022Quote: ... Both the protein extracts from cell pellets and the supernatants from cultured cells were subjected to protein quantification using BCA Protein Assay Kit (Thermofisher). Samples were boiled at 99 °C for 2 min ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Total protein concentrations of the samples were determined by the Pierce™ bicinchoninic acid (BCA) protein assay (Thermofisher Scientific, USA), and samples were aliquoted and stored at −80°C until further use ...
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bioRxiv - Cell Biology 2022Quote: Total proteins were measured in the serum of the toads using a BCA protein quantification kit (Thermo Fisher, Cat 23225).
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bioRxiv - Molecular Biology 2022Quote: ... The lysed supernatant was obtained by centrifugation at 4°C for 10 min at 13,000 rpm and the total protein content was quantified by a Micro BCA Protein assay kit (Thermo Scientific). The proteins were boiled in Laemmli SDS sample buffer (Alfa Aesar ...
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bioRxiv - Physiology 2022Quote: Protein concentration of cell lysates was determined using the PierceTM BCA protein assay kit according to manufacturer’s instructions (ThermoFisher Scientific). Samples were subsequently subjected to SDS-PAGE and blotted on a nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2022Quote: ... Total protein concentration was subsequently determined using the fluorescence-based EZQ™ Protein Quantitation Kit (Life Technologies, Carlsbad, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... The concentration of the protein was determined using the Pierce BCA Protein Assay Kit (cat. No. 23225; Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2022Quote: ... Extracted protein from the cell-only control samples was quantified using Pierce Rapid Gold BCA Protein Assay Kit (Thermo Fisher). 10 µg protein from each sample was then analyzed by sodium dodecyl sulfate– polyacrylamide gel electrophoresis (SDS-PAGE ...
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Mechanism of the ANT-mediated transport of fatty acid anions across the inner mitochondrial membranebioRxiv - Biophysics 2022Quote: The protein concentration of the proteoliposomes was measured with the Micro BCATM Protein Assay Kit (Thermo Fisher Scientific, Prod. #23235). SDS-PAGE and silver staining verified protein purity ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell debris were pelleted by centrifugation (14,000g, 10min, 4°C) and whole cell protein extract concentration was measured using Qubit Protein Assay Kit (Thermo Fisher). Protein extracts were immediately used for EnzyChrom Catalase Assay Kit and EnzyChrom Glutathione Reductase Assay Kit (BioAssay Systems) ...
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bioRxiv - Developmental Biology 2022Quote: Protein synthesis was detected by the Click-iT® Plus OPP Alexa Fluor 594 Protein Synthesis Assay Kit (Molecular Probes) as previously described (Sanchez et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration was measured with BCA Protein Assay Kit according to the manufacturer’s instructions (Thermo Fisher Scientific, Carlsbad, CA, USA). Then ...
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bioRxiv - Cell Biology 2022Quote: Surface proteins on Caco-2 cells were biotinylated using Pierce Cell Surface protein biotinylation and Isolation Kit (Thermo Scientific, A44390) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Total protein concentration in each sample was quantified using Pierce 660nM protein assay reagent (Thermo Fisher Scientific, Woolwich, UK, #22662) supplemented with ionic detergent compatibility reagent (IDCR ...
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bioRxiv - Cell Biology 2022Quote: Protein complexes from the eluate were resolved by SDS-PAGE and stained with Coomassie blue (Imperial Protein Stain, ThermoFisher Scientific). Protein bands of interest between 250 kDa and 20 kDa were excised ...