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Citations for Thermo Fisher :
7401 - 7450 of 10000+ citations for Recombinant Human IL18 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... IL-1β was evaluated using an IL-1 beta Human ELISA Kit (Invitrogen). Neutrophils (3 × 105/well ...
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bioRxiv - Immunology 2024Quote: Human and murine B cells were lysed in RIPA buffer (Thermo Fisher, #89900) supplemented with protease inhibitors (Roche ...
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bioRxiv - Immunology 2024Quote: ... Isotype-specific antibodies were detected with goat anti-human IgG (1/1000, Invitrogen) or IgM (1/2500 ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were activated with DynaBeads Human T-Activator CD3/CD28 (Thermo Fisher, 11131D) at a 1:1 cell-to-bead ratio and cultured in 24-well plates with 1 million cells per mL at 37◦C and 5% CO2 overnight.
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bioRxiv - Immunology 2024Quote: ... in combination (each at 100ng/mL) or with Dynabeads human T-Activator (ThermoFisher) anti-CD3/CD28 beads (at a 1:1 bead/cell ratio) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... followed by secondary staining with a Goat anti-human IgG Fc (ThermoFisher A18818). Individual bEFNB2 expressing cells were then sorted into individual wells of a 96 well plate on a Sony MA900 and gated to include only live ...
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bioRxiv - Molecular Biology 2024Quote: ... the CECs were resuspended in stabilisation media (containing Human Endothelial-SFM (Life technologies) supplemented with 5% foetal bovine serum (FBS) ...
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bioRxiv - Immunology 2024Quote: ... ELISA plates were coated with anti-human IgG (ThermoFisher 88-50550-88, 1X) and blocked overnight with 2X assay buffer (Invitrogen 88-50550-88 ...
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bioRxiv - Immunology 2022Quote: ... The residual amount of endotoxin in the recombinant S100A4 preparation was examined using a Pierce™ Chromogenic Endotoxin Quant Kit (Thermo Fisher Scientific). Mice were anesthetized using isoflurane prior to immunization ...
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bioRxiv - Immunology 2021Quote: ... Purified digested DNA fragments were cloned into human Igγ1- and Ig κ-/ Igλ-expressing vectors 38 and recombinant IgG1 antibodies were produced by transient co-transfection of Freestyle™ 293-F suspension cells (Thermo Fisher Scientific) using PEI-precipitation method as previously described 39 ...
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bioRxiv - Plant Biology 2020Quote: The interaction of the NAB1 promoter fragments with recombinant LCRF-SBP (rLCRF-SBP) was analyzed by using the LightShift®Chemiluminescent EMSA Kit (Thermo Scientific™), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: Recombinant bacmids containing the gene of interest (PDI) were generated according to the Baculovirus Expression system manual (Thermo Fisher Scientific, Cat. No. 10359016). In brief ...
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bioRxiv - Microbiology 2022Quote: Cu-sensitive strains harboring the recombinant pTR plasmids were harvested for metal content determination by using inductively coupled plasma mass spectrometry (ICP-MS) (Thermo Fisher Scientific, USA). E ...
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bioRxiv - Molecular Biology 2020Quote: ... The linearized recombinant plasmid was used as templates to synthesize capped RNAs (cRNAs) with the mMessage mMachine T7 Kit (Life Technologies, Carlsbad, CA). The cRNAs were dissolved in RNase-free water at a concentration of 1000 ng/μL ...
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bioRxiv - Developmental Biology 2021Quote: ... Preprotech, cat#100- 18B) and 50 ng/mL recombinant sonic hedgehog (SHH, Preprotech, cat#100-45) in Knockout DMEM (Gibco cat #10829-018), 10% Knockout serum replacement (KOSR ...
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bioRxiv - Microbiology 2019Quote: ... and then stained with anti-LCMV-WE recombinant NP immunized rabbit serum and HRP-goat anti-rabbit IgG (H+L) DS Grd (Life Technologies, Carlsbad, CA) (41) ...
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bioRxiv - Microbiology 2021Quote: ... ExpiSf9 cells for recombinant baculovirus production were cultured at 27°C and 125 rpm in ExpiSf CD Medium (Thermo Fisher Scientific, Waltham, MA). B19V-infected plasma sample was obtained from a donation center (CSL Behring AG ...
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bioRxiv - Microbiology 2019Quote: ... and stained with anti-LCMV-WE recombinant NP immunized rabbit serum and HRP-goat anti-rabbit IgG (H+L) DS Grd (lot: 917439A, Life Technologies, Carlsbad, CA) (41) ...
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bioRxiv - Cell Biology 2021Quote: ... were delivered into H9 cells along with recombinant True Cut Cas9 v2 using the Neon Transfection system (Thermo Fisher Scientific, Cat. No MPK5000). H9 cells (1×105 cells ...
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bioRxiv - Microbiology 2022Quote: ... Samples were washed in blocking buffer thrice and stained with 1:1000 dilution of Alpaca anti-Rabbit IgG Nano (VHH) Recombinant Secondary Antibody conjugated with Alexa Fluor™ 647 (Invitrogen SA5-10327), Goat anti-Mouse IgG conjugated with Alexa Fluor™ Plus 488 (5 μg/mL final concentration ...
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bioRxiv - Neuroscience 2022Quote: ... The injections were either a fluorescent dextran amine or a recombinant cholera toxin subunit B tracer: (Dextran-405; CTB-488, CTB-594, CTB-647, Invitrogen #C22841, #C34777, #C34778). We chose our injection volume based on the size ...
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bioRxiv - Genomics 2022Quote: ... The mini prep DNA was then used with the Bac-to-Bac™ expression system to generate a recombinant bacmid as per manufacturer conditions with MAX efficiency™ DH10Bac™ Competent Cells (ThermoFisher). After 4 hours of transformation recovery ...
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bioRxiv - Microbiology 2023Quote: ADCP was measured using a flow cytometry-based phagocytosis assay.98 Biotinylated recombinant HIV-1 gp120 from strain CN54 (Acro Biosystems; catalog #GP0-V182E6) was combined with fluorescent NeutrAvidin beads (Life Technologies; catalog #F8776) at a concentration of 1 µg protein per µl bead and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... 500 nM recombinant MEC-12/MEC-7 tubulins and a kinase candidate [500 nM recombinant MBK-2m or 475 nM CDK1/Cyclin B (Thermo Fisher; Cat# PV3292)] were incubated in MEM buffer [100 mM MES ...
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bioRxiv - Microbiology 2023Quote: ... the binding of HIV+ plasma was detected using a biotin-tagged dimeric recombinant soluble FcγRIIIa (0.2 μg/mL) followed by the addition of Alexa Fluor 647-conjugated streptavidin (Thermo Fisher Scientific; 2 μg/mL). Infected cells were then stained intracellularly for HIV-1 p24 ...
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Development of monoclonal antibody-based blocking ELISA for detecting SARS-CoV-2 exposure in animalsbioRxiv - Microbiology 2023Quote: MARC-145 cells transfected with the recombinant pCAGGS-N plasmid were lysed in Pierce™ IP Lysis Buffer (Thermo Fisher Scientific, Waltham, MA), and then mixed with each of the purified anti-N mAbs ...
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bioRxiv - Cancer Biology 2022Quote: One million of BMDCs cultures in the absence or presence of 10 ng/ml of recombinant MDK (indicate provider and origin) were incubated with 1 μg/ml of Ovalbumin-AF594 conjugate (Thermo Fisher Scientific; O34781) for 30 min at 37°C ...
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bioRxiv - Immunology 2024Quote: ... pH 7.34 for a short stimulation of 30 min) or in a CBMC medium supplemented with recombinant h-SCF (100 ng/mL, Gibco, PeproTech, #300-07) (for a longer stimulation) ...
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bioRxiv - Plant Biology 2020Quote: ... The protein content of the supernatant was quantified using Ready-to-use Coomassie Blue G-250 Protein Assay Reagent (ThermoFisher) and Albumin Standard 23209 (ThermoFisher).
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bioRxiv - Plant Biology 2019Quote: ... GTPase activity of native and mutant Gα proteins were detected with or without an RGS protein using the ENZchek phosphate assay kit (Invitrogen) as previously reported (Roy Choudhury et al. ...
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bioRxiv - Cancer Biology 2021Quote: The cell membrane protein samples were collected using Mem-PER eukaryotic membrane protein extraction reagent kit (Thermo Scientific, 89826, MA) according to the protocol instruction ...
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bioRxiv - Biophysics 2021Quote: ... The crude lysates were cleared by centrifugation at 13,000 rpm for 15 min and protein content was analyzed by Pierce BCA protein assay (Thermo Scientific).
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bioRxiv - Cancer Biology 2021Quote: ... After clearing by centrifugation at 13,300□rpm for 10□min at 4°C the protein concentration was determined using Coomassie Plus Protein Assay (ThermoFisher Scientific). Protein concentrations were adjusted to a final concentration of 2mg/ml followed by denaturation upon addition of 4X sample buffer containing 10% Beta-mercaptoethanol and heating to 95oC for 5 minutes ...
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bioRxiv - Cancer Biology 2021Quote: Subcellular protein fractionation was performed either by using Subcellular Protein Fractionation Kit for Cultured Cells (Thermo Scientific; Cat. no. 78840) according to the manufacturer protocol or following the protocol below.
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bioRxiv - Cell Biology 2020Quote: ... The eluted proteins were separated on a 4-12% NuPAGE gel (Novex) and stained with Imperial protein stain (Thermo Scientific). The proteins of interest were excised for identification by MS.
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bioRxiv - Cell Biology 2019Quote: Cells were lyzed using RIPA buffer and proteins were quantified using the BCA method (Protein BCA Assay Kit, Thermo Fisher). The protein extracts were separeted on 12% SDS-PAGE gels (50 μg of total proteins were loaded per well ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was then incubated with appropriate antibodies for 12 h and then conjugated with dynabeads Protein A or Protein G beads for an additional 1 h SFB- or GFP-tagged proteins were immunoprecipitated with streptavidin dynabeads (Invitrogen) or GFP-Trap (Chromotek) ...
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bioRxiv - Cell Biology 2020Quote: ... Total concentration of protein in lysate was quantified using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, 23225) measured at 562 nm and compared to a standard curve ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was transferred to a new tube and total protein was quantified using EZQ® Protein Quantitation kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Protein in testes and cultured cells were extracted using T-PER Tissue protein extraction reagent (Thermo Fisher Scientific, Waltham, Massachusetts), subjected to SDS-PAGE ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were pelleted and 50μg of protein was quantified by ionic detergent compatible Pierce 660nm Protein Assay Reagent (Thermo Fisher) in 8M Urea ...
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bioRxiv - Biochemistry 2022Quote: ... Total protein content of each pre-cleared sample was determined using Pierce bicinchoninic acid assay protein assay (Thermo Fisher Scientific). To measure luciferase activity ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations were then measured by bicinchoninic acid (BCA) assay (Micro BCA™ Protein Assay Kit, Cat # 23235; Thermo Scientific). The resulting supernatant yielded low volumes (30-40 µl of supernatant ...
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bioRxiv - Molecular Biology 2020Quote: ... The suspension was incubated at 25°C for an additional 2 hr with Protein G SepharoseTM 4 Fast Flow or DynabeadsTM Protein G (Invitrogen), pre-blocked with 1 mg/ml BSA and 0.25 mg/ml Salmon Sperm DNA ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein concentrations of the final purified samples were measured with Pierce™BCA Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... Protein samples were separated on a NuPAGE™ 4–12% Bis-Tris Protein Gel (Thermo Fisher Scientific, Bleiswijk, the Netherlands) and transferred to an Immobilon-FL PVDF membrane (Merck ...
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bioRxiv - Genomics 2019Quote: ... Soluble material was incubated with 3-5 μg of antibody bound to 50 μl protein A or protein G Dynabeads (Invitrogen) and incubated overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... Protein concentration was determined relative to a BSA standard using the PierceTM BCATM Protein-Assay (Thermo Scientific, Waltham, MA, USA). Lysates were finally adjusted to a final concentration of 2 µg/µl with 4X Laemmli buffer (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... and CCL21 levels were normalized to total protein concentration of the homogenates determined using Pierce BCA protein Assay kit (ThermoFisher).
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bioRxiv - Molecular Biology 2021Quote: ... Purity was determined by SDS-PAGE and protein concentrations were determined using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). For generation of POM1 mutants ...