Labshake search
Citations for Thermo Fisher :
7501 - 7550 of 10000+ citations for 5 Alpha dihydroprogesterone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... resuspended in pre-sort buffer containing 3-5 nM SYTOX Green Nucleic Acid Stain (Thermo Fisher Scientific), and incubated for 20 min at 4°C ...
-
bioRxiv - Bioengineering 2022Quote: ... and blocked for 1 hr with a solution containing 0.5% TritonX-100 and 5% donkey serum (ThermoFisher). Primary antibodies used were rat CD68 (FA-11 ...
-
bioRxiv - Developmental Biology 2022Quote: ... washed five times for 30 minutes each with PBS and incubated with 5% normal goat serum (ThermoFisher) and 0.5% TritonX (Sigma Aldrich ...
-
bioRxiv - Developmental Biology 2022Quote: PCR products were used as templates for T7 transcription reactions with the 5× MEGAscript T7 kit (Ambion). dsRNA was injected dorsally in 0-1 hour old embryos from the stocks w ...
-
bioRxiv - Biochemistry 2022Quote: ... and both were cultured at 37°C under 5% CO2 in Dulbecco’s modified Eagles medium (DMEM, Gibco) supplemented with 10% (vol/vol ...
-
bioRxiv - Immunology 2022Quote: ... 5 mL of MEM medium with 10% FBS and 1X Antibiotic-Antimycotic (Thermo Fisher Scientific, cat: 15240062) was added directly ...
-
bioRxiv - Biochemistry 2022Quote: ... HEK293-PSMB2-HB cells were transfected with 5 μg of the cloned plasmid using Lipofectamine 3000 (Invitrogen). After 48 h incubation ...
-
bioRxiv - Cancer Biology 2022Quote: ... The 5-µm FFPE tissue section was mounted on positively charged Superfrost glass slide (Thermo Fisher Scientific) and baked for 30 mins at 60 °C ...
-
bioRxiv - Genomics 2022Quote: ... These cells were cultured at 37°C and 5% CO2 in reprogramming medium containing KnockOut DMEM (GIBCO), 10% knockout serum replacement (KSR ...
-
bioRxiv - Developmental Biology 2022Quote: ... single cell suspensions were centrifuged and resuspended in 5 mL of red blood cell lysis buffer (Invitrogen) for 5 min ...
-
bioRxiv - Cell Biology 2022Quote: ... Antibodies for immunofluorescent staining were as follows: vinculin (clone VIN-11-5, Sigma or clone 42H89L44, Invitrogen); phosphotyrosine (clone 4G10 ...
-
bioRxiv - Developmental Biology 2022Quote: ... at 95°C for 5 min and separated using NuPAGE 4%–12% Bis-Tris Protein Gel (Invitrogen) in NuPAGE MES SDS Running Buffer (Invitrogen) ...
-
bioRxiv - Cancer Biology 2022Quote: ... real-time quantitative PCR was done using the ABI Quant Studio 5 and 6 (Life technologies, USA). The cDNA was used as template and DyNAmo Flash SYBR Green qPCR kit (#F-416L ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated at 37°C in humidified 5% CO2 incubators (Eppendorf or Thermo Fisher Scientific, USA).
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were maintained at 37°C 5% CO2 in Dulbecco’s MEM (Life Technologies, ThermoFisher Scientific, Waltham MA) with 10% FBS ...
-
bioRxiv - Cell Biology 2022Quote: ... about ∼5 μg of bacmid were transfected using 6 μl of Cellfectin II reagent (Thermo Fisher Scientific). 5 days after initial transfection ...
-
bioRxiv - Cancer Biology 2022Quote: ... MS-5 cells were seeded into 0.2% gelatin coated 96 well-flat bottom plates (Thermo Fisher, 167008) at a density of 4-5×105 cells per well in Myelocult medium (H5100 ...
-
bioRxiv - Neuroscience 2021Quote: ... The template cDNA was reverse transcribed with 5’-RACE RT primer and SuperScript III reverse transcriptase (Invitrogen), followed by poly(A ...
-
bioRxiv - Developmental Biology 2020Quote: ... Tissue was dissociated for 5 minutes at 37°C in TrypLE Express Enzyme reagent (Thermo Fisher 12604013), with shaking ...
-
bioRxiv - Microbiology 2020Quote: 5×105 293T cells were seeded and the next day transfected using Lipofectamine 3000 (Thermo Fisher Scientific) with 3 μg of E.V. ...
-
bioRxiv - Immunology 2020Quote: ... 0.1 μl of External RNA Controls Consortium (ERCC) spike-in mix (10-5 diluted; Life Technologies, 4456740) was added with 0.1 μl of 100 μM oligo-dT primer and 1 μl of dNTP mix and 0.8 μl of H2O ...
-
bioRxiv - Biophysics 2020Quote: ... A 5% solution of 0.2 μm diameter red fluorescent carboxylate-modified microspheres (FluoSpheres, Invitrogen, Carlsbad, CA, USA) in PBS was vortexed for 10 seconds ...
-
bioRxiv - Developmental Biology 2021Quote: ... and qRT-PCR performed with SYBR green (KAPA) on a QuantStudio 5 qPCR machine (Thermo Fisher Scientific). qRT-PCR data were normalised to two housekeeping genes (Rpl7/H2A) ...
-
bioRxiv - Microbiology 2021Quote: ... the bottom part which closely fit the microscopy glass slide (24 x 40 mm, #5; Thermo scientific) and contained grooves to guide positioning of the top part precisely above the microfluidic device ...
-
bioRxiv - Microbiology 2020Quote: HapMap LCLs (Coriell Institute) were cultured at 37°C in 5% CO2 in RPMI 1650 media (Invitrogen) supplemented with 10% FBS (Thermo-Fisher) ...
-
bioRxiv - Microbiology 2020Quote: ... 6-carboxyfluorescein (FAM)-5= CCG TCA ATC AAG GAG CGC CTC 3=-6 carboxytetramethylrhodamine (TAMRA) (Life Technologies).Viral RNA was detected using the CFX96 detection system (Bio-Rad Laboratories ...
-
bioRxiv - Molecular Biology 2020Quote: ... The sheath gas pressure and the auxiliary gas pressure were 30 and 5 (Thermo Fisher arbitrary units) respectively ...
-
bioRxiv - Plant Biology 2021Quote: ... a standard PCR (RT-PCR) was then performed using DreamTaq DNA polymerase (5 U/μL) (Thermo Scientific). VIGS-CHUP1 silencing was confirmed by separately amplifying CHUP1a and CHUP1b from cDNA with the following primer pairs:
-
bioRxiv - Biochemistry 2021Quote: ... HepG2 cells were cultured at 37°C in 5% CO2 in DMEM (GIBCOTM, Invitrogen Corp., Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Microbiology 2021Quote: ... 6-carboxyfluorescein (FAM)-5’ CCG TCA ATC AAG GAG CGC CTC 3’-6 carboxytetramethylrhodamine (TAMRA) (Life Technologies).Viral RNA was detected using the CFX96 detection system (Bio-Rad Laboratories ...
-
bioRxiv - Biophysics 2021Quote: ... trypsinized for 3 mins with 5 ml of an EDTA/Trypsin solution (0.05 %, 59417C, Gibco, Thermo Fisher), and diluted with low glucose DMEM (Dulbecco’s modified Eagle’s medium ...
-
bioRxiv - Microbiology 2021Quote: ... purified His-RBD was diluted to 200 μg/mL in PBS buffer containing Sypro Orange 5× (ThermoFisher) in a 96-well white PCR plate ...
-
bioRxiv - Microbiology 2021Quote: ... The 20 µl reaction volume consisted of 5 µl Taqman Fast Advanced Mastermix 4x (Thermo Fisher Scientific), 0.4 μl of tat 2.0 forward primer and rev reverse primer (each at 20 µM ...
-
bioRxiv - Developmental Biology 2021Quote: Cells were maintained pluripotent and cultured at 37°C in 5% CO2 in 25 cm2 flasks (Nunc) coated with 0.1% gelatin (Sigma ...
-
bioRxiv - Molecular Biology 2020Quote: NIH/3T3 cells were cultured at 37 °C and 5% CO2 in DMEM high glucose (Gibco, 41965039) supplemented with 10% FBS (Gibco ...
-
bioRxiv - Immunology 2020Quote: ... centrifuged at 14,000 RPM for 5 min at 4°C and resuspended in UltraPure H2O (Ambion, UK). Purified transcripts were capped using the ScriptCap™ Cap 1 Capping System Kit (CellScript ...
-
bioRxiv - Cell Biology 2021Quote: Edited U937 cells were washed in PBS with 5% heat-inactivated fetal bovine serum (Gibco 10082-147), resuspended at 106 cells/mL and then treated with Human TruStain FcX blocker (BioLegend 422302) ...
-
bioRxiv - Neuroscience 2021Quote: ... diluted 5% volume to volume in paraffin oil (Fluka Analytical, 76235) and acetic acid (Fisher Scientific, A385) diluted in 5% volume to volume in water — using a stimulus controller (Ockenfels Syntech ...
-
bioRxiv - Plant Biology 2021Quote: ... 5-DAG seedlings were incubated in the dark in 10 μg/mL PI (Invitrogen, cat. n. P4170) for 10 min and then rinsed twice with water and observed under a Zeiss LSM 700 confocal microscope (Zeiss ...
-
bioRxiv - Microbiology 2020Quote: ... flanked by 5’ Nhel/3’Apal sites for subcloning into the pcDNA3.1(+) vector (Thermo Fisher Scientific, USA). HEK293T cells and Vero E6 cells were purchased from ATCC ...
-
bioRxiv - Biophysics 2021Quote: ... cell nuclei were stained by exposure for 5 min to 1 mg/100 ml Hoechst 33342 (ThermoFisher) in water.
-
bioRxiv - Bioengineering 2021Quote: ... The paraffin block was cut into 5-µm sections and placed on Superfrost Plus slides (Thermo Fisher). Sections were dried overnight and then subjected to immunohistochemistry (IHC ...
-
bioRxiv - Microbiology 2021Quote: ... Quantitative real-time PCR analysis was performed in a QuantStudio 5 Real-Time PCR System (Applied Biosystems). Primers and TaqMan probes were as follows ...
-
bioRxiv - Biochemistry 2021Quote: ... 2′-(or-3′)-O-(N-Methylanthraniloyl) adenosine 5′-triphosphate (mant-ATP, Thermo-Fisher Scientific, Waltham, MA, USA) was serially diluted to concentrations of 5 -15 μM in assay buffer and added to the myosin at a final concentration of 1X -1.2X the final concentration of myosin ...
-
bioRxiv - Neuroscience 2020Quote: ... and 5 μg/mL human recombinant laminin 521 (BioLamina #LN521-02) in E8 basal medium (Gibco #A1517001) supplemented with 1% Penicillin/Streptomycin (Pen/Strep ...
-
bioRxiv - Microbiology 2021Quote: ... 5∼15 ng DNA per reaction in 384-well Q-PCR plates (Applied Biosystems™ Cat. # 4483285) with total 12 μL reaction ...
-
bioRxiv - Biochemistry 2020Quote: ... The samples were loaded onto a trapping column (Thermo Scientific, PepMap100, C18, 300 μm X 5 mm), using partial loop injection ...
-
bioRxiv - Biophysics 2020Quote: ... MEFs co-expressing FHL2-eGFP and Lifeact-mApple were incubated with 5 μg/mL Hoechst (Invitrogen, H1399) for 1 hour ...
-
bioRxiv - Immunology 2021Quote: ... Lung and brain sections were washed twice for 5 min and stained with DAPI (Life technologies, #D1360) for 5 min at RT ...
-
bioRxiv - Genetics 2021Quote: ... individual myofibres were isolated by enzymatic digestions and then loaded with 5 µM fluo-4AM (Molecular Probes) for 45 min at room temperature in a normal rodent Ringer’s solution consisting of (in mM) ...